Click here to switch to the map view.

The map label for this gene is ispF

Identifier: 238921116

GI number: 238921116

Start: 3133163

End: 3133636

Strand: Reverse

Name: ispF

Synonym: NT01EI_3255

Alternate gene names: 238921116

Gene position: 3133636-3133163 (Counterclockwise)

Preceding gene: 238921117

Following gene: 238921115

Centisome position: 82.2

GC content: 62.03

Gene sequence:

>474_bases
ATGAGAATTGGTCACGGTTTTGACGTGCACCGCTTCGGCGGCGAGGGGCCGCTGATTATCGGCGGTGTGCGCATCGCGCA
TCCACAGGGGCTGCTGGCCCATTCCGATGGTGATGTCGCTCTGCATGCGGCGACTGATGCCCTGTTGGGGGCGGCGGCCC
TGGGGGATATTGGCAAGCTGTTCCCGGATACGGATCCCGCCTATAAGGGGGCGGACAGCCGCGCGCTGCTGCGTGAGGCA
TACCGCAGCATTCGTGCCAGGGGCTACCGCCTGGGTAATCTGGATATTACCCTGATTGCCCAGGCACCCAAAATGGCGCC
GCACATTCCGCAGATGCGGGTGTTTCTGGCCGAAGATTTACAGTGCCCGATTGATGATGTCAACGTGAAGGCGACCACCA
CTGAAAAGCTGGGCTTTACCGGGCGTGGGGAGGGGATCGCCTGCGCAGCGGTGGCCCTGCTGATCAAGGAGTAA

Upstream 100 bases:

>100_bases
TTCAGATAATATTAAAGTTACCCGTCCGGAGGATTTGGCGCTGGCAGAGTTCTATCTGGCGCGTCATGCCTTTTTGACGC
ACTCACAGGAGCAATAACCG

Downstream 100 bases:

>100_bases
ACGATGGAGTTTTCTACGCTGCAGTATCTGCATGGGCGCCCGGTGGCGACCGGGGTGGTCAAAGCCTTTGCCGAGGATTT
TTTTGTTGCAGAGGATCTGG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRIGHGFDVHRFGGEGPLIIGGVRIAHPQGLLAHSDGDVALHAATDALLGAAALGDIGKLFPDTDPAYKGADSRALLREA
YRSIRARGYRLGNLDITLIAQAPKMAPHIPQMRVFLAEDLQCPIDDVNVKATTTEKLGFTGRGEGIACAAVALLIKE

Sequences:

>Translated_157_residues
MRIGHGFDVHRFGGEGPLIIGGVRIAHPQGLLAHSDGDVALHAATDALLGAAALGDIGKLFPDTDPAYKGADSRALLREA
YRSIRARGYRLGNLDITLIAQAPKMAPHIPQMRVFLAEDLQCPIDDVNVKATTTEKLGFTGRGEGIACAAVALLIKE
>Mature_157_residues
MRIGHGFDVHRFGGEGPLIIGGVRIAHPQGLLAHSDGDVALHAATDALLGAAALGDIGKLFPDTDPAYKGADSRALLREA
YRSIRARGYRLGNLDITLIAQAPKMAPHIPQMRVFLAEDLQCPIDDVNVKATTTEKLGFTGRGEGIACAAVALLIKE

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=85.2564102564102, Blast_Score=249, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_EDWI9 (C5BGJ0)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934631.1
- GeneID:   7961088
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3255
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 16603; Mature: 16603

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGHGFDVHRFGGEGPLIIGGVRIAHPQGLLAHSDGDVALHAATDALLGAAALGDIGKL
CCCCCCCEEEEECCCCCEEEEEEEEECCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHH
FPDTDPAYKGADSRALLREAYRSIRARGYRLGNLDITLIAQAPKMAPHIPQMRVFLAEDL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCEEEEEECCCCCCCCCCHHHHEEHHCC
QCPIDDVNVKATTTEKLGFTGRGEGIACAAVALLIKE
CCCCCCCEEEEECHHHCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRIGHGFDVHRFGGEGPLIIGGVRIAHPQGLLAHSDGDVALHAATDALLGAAALGDIGKL
CCCCCCCEEEEECCCCCEEEEEEEEECCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHH
FPDTDPAYKGADSRALLREAYRSIRARGYRLGNLDITLIAQAPKMAPHIPQMRVFLAEDL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCEEECCCEEEEEECCCCCCCCCCHHHHEEHHCC
QCPIDDVNVKATTTEKLGFTGRGEGIACAAVALLIKE
CCCCCCCEEEEECHHHCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA