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The map label for this gene is ispD [H]

Identifier: 238921117

GI number: 238921117

Start: 3133640

End: 3134371

Strand: Reverse

Name: ispD [H]

Synonym: NT01EI_3256

Alternate gene names: 238921117

Gene position: 3134371-3133640 (Counterclockwise)

Preceding gene: 238921118

Following gene: 238921116

Centisome position: 82.22

GC content: 63.66

Gene sequence:

>732_bases
ATGAACGATAACGACTCCGTTAATGAACCGTCCATTGTCGCCATTGTACCGGCAGCCGGGATCGGCAGCCGGATGCAAAG
CGCTACCCCCAAGCAATACCTCATGCTGAATGGCAAGACCATTCTGGAGCATGCCGTGACGGCGCTGTTTGCCCATCCGG
CTGTGCGCCGTGCGATCGTTGCGCTGCACCCGCAGGATGCCTGCTTTGACGCGCTGCCGCTGGCGGTGGATCGGCGGGTC
AGCCGCGTGACGGGCGGGGCGACACGCGCCGAGTCGGTGCTTGCTGCGCTGCTGGCCGCCGACGATGCCGACTGGGTCCT
GGTGCACGATGCGGCGCGTCCCTGCCTGCAGTCTGGCGATCTGGCGCATCTGGTGGCCTGTATGGACGCCGCGCAGCAGG
GGGCGATATTGGCGACGCCGGTCTGCGACACGATGAAACGGGCTGAAGCCGGTCTCCCGCAGATCGCCCATACGGTGGAG
CGCCAGGATCTGTGGCATGCGCTGACGCCGCAGCTGTTTCCCCGGGCGCTGCTGATCCAGTGTCTGCAGCGTGCTCTGAG
CGAGGGGGCAACGGTGACCGATGAGGCCTCGGCGCTGGAGTATTGCGGCTATCATCCGCGCTTGGTCAGCGGGCGTTCAG
ATAATATTAAAGTTACCCGTCCGGAGGATTTGGCGCTGGCAGAGTTCTATCTGGCGCGTCATGCCTTTTTGACGCACTCA
CAGGAGCAATAA

Upstream 100 bases:

>100_bases
CTTCCCAGTCAGTCGCATCCTACCCGTCAGTGACGGCGAGTCACTGAATTCTTTGCATGGCCTGCGCCATGTCTTCATAT
CCGATAACGATAATTCACCG

Downstream 100 bases:

>100_bases
CCGATGAGAATTGGTCACGGTTTTGACGTGCACCGCTTCGGCGGCGAGGGGCCGCTGATTATCGGCGGTGTGCGCATCGC
GCATCCACAGGGGCTGCTGG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MNDNDSVNEPSIVAIVPAAGIGSRMQSATPKQYLMLNGKTILEHAVTALFAHPAVRRAIVALHPQDACFDALPLAVDRRV
SRVTGGATRAESVLAALLAADDADWVLVHDAARPCLQSGDLAHLVACMDAAQQGAILATPVCDTMKRAEAGLPQIAHTVE
RQDLWHALTPQLFPRALLIQCLQRALSEGATVTDEASALEYCGYHPRLVSGRSDNIKVTRPEDLALAEFYLARHAFLTHS
QEQ

Sequences:

>Translated_243_residues
MNDNDSVNEPSIVAIVPAAGIGSRMQSATPKQYLMLNGKTILEHAVTALFAHPAVRRAIVALHPQDACFDALPLAVDRRV
SRVTGGATRAESVLAALLAADDADWVLVHDAARPCLQSGDLAHLVACMDAAQQGAILATPVCDTMKRAEAGLPQIAHTVE
RQDLWHALTPQLFPRALLIQCLQRALSEGATVTDEASALEYCGYHPRLVSGRSDNIKVTRPEDLALAEFYLARHAFLTHS
QEQ
>Mature_243_residues
MNDNDSVNEPSIVAIVPAAGIGSRMQSATPKQYLMLNGKTILEHAVTALFAHPAVRRAIVALHPQDACFDALPLAVDRRV
SRVTGGATRAESVLAALLAADDADWVLVHDAARPCLQSGDLAHLVACMDAAQQGAILATPVCDTMKRAEAGLPQIAHTVE
RQDLWHALTPQLFPRALLIQCLQRALSEGATVTDEASALEYCGYHPRLVSGRSDNIKVTRPEDLALAEFYLARHAFLTHS
QEQ

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=222, Percent_Identity=65.3153153153153, Blast_Score=294, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 26183; Mature: 26183

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDNDSVNEPSIVAIVPAAGIGSRMQSATPKQYLMLNGKTILEHAVTALFAHPAVRRAIV
CCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
ALHPQDACFDALPLAVDRRVSRVTGGATRAESVLAALLAADDADWVLVHDAARPCLQSGD
HCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCC
LAHLVACMDAAQQGAILATPVCDTMKRAEAGLPQIAHTVERQDLWHALTPQLFPRALLIQ
HHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHH
CLQRALSEGATVTDEASALEYCGYHPRLVSGRSDNIKVTRPEDLALAEFYLARHAFLTHS
HHHHHHHCCCCCCCHHHHHHHCCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHCC
QEQ
CCC
>Mature Secondary Structure
MNDNDSVNEPSIVAIVPAAGIGSRMQSATPKQYLMLNGKTILEHAVTALFAHPAVRRAIV
CCCCCCCCCCCEEEEECCCCCCHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHH
ALHPQDACFDALPLAVDRRVSRVTGGATRAESVLAALLAADDADWVLVHDAARPCLQSGD
HCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCC
LAHLVACMDAAQQGAILATPVCDTMKRAEAGLPQIAHTVERQDLWHALTPQLFPRALLIQ
HHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHH
CLQRALSEGATVTDEASALEYCGYHPRLVSGRSDNIKVTRPEDLALAEFYLARHAFLTHS
HHHHHHHCCCCCCCHHHHHHHCCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHCC
QEQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA