Click here to switch to the map view.

The map label for this gene is truD

Identifier: 238921115

GI number: 238921115

Start: 3132113

End: 3133159

Strand: Reverse

Name: truD

Synonym: NT01EI_3254

Alternate gene names: 238921115

Gene position: 3133159-3132113 (Counterclockwise)

Preceding gene: 238921116

Following gene: 238921114

Centisome position: 82.19

GC content: 63.13

Gene sequence:

>1047_bases
ATGGAGTTTTCTACGCTGCAGTATCTGCATGGGCGCCCGGTGGCGACCGGGGTGGTCAAAGCCTTTGCCGAGGATTTTTT
TGTTGCAGAGGATCTGGGATTTACCCCGGACGGCGAGGGTGAGCATGTGCTGGTTCGCCTGCGTAAAGAGGGGTGTAATA
CCCAGTTTGTGGCGGAGAATCTGGCCCGCTTTGCCCGTATCGCCGGGCGTTCGGTCAGCTACGCCGGGCTGAAGGATCGT
CATGCGGTGACCGAGCAGTGGTTCTGCCTGCATCTACCGGGGCGTGATACGCCAGACTTCAGCCAGTTTGTGCTGGAGGG
ATGTCAGGTTCTGGCCGTGGCCCGCCATCGAAAGAAGCTGCGTATCGGTACGCTGCGTGGCAACCAGTTCCGCCTGGTGC
TGCGTCACATCAGTGACAGCGCCGAGGTCGAAGAGCGCCTGCGCCGGGTGGCGACGCAGGGTGTGCCCAACTATTTTGGC
GAGCAGCGCTTTGGCCGTGGCGGTAATAATCTGCAGCAGGCGCGTCGCTGGGCCACCGATCAGATTCGGGTGAAAGAGCG
CGCCAAGCGCGGTTTCTATCTCTCCGCGGCGCGCAGCGCGCTGTTTAATTTGTTGGTAAGTGAGCGGCTGGCTGCGGGTT
GTTTTTCTCGGGTTCTGGCGGGCGACGCGCTGCAGCTGACCGGTCGCGGCAGCTGGTTTGTCGCCCGGGAGGCGGAGCTG
TCGGTGCTGCAGCAGCGGCTGGACGTCGGCGAGCTGCGCATTACGGCGGCGCTGCCGGGCGACGGTGACGGGGGCGTCGG
CGGCGATGCGGCAGAGTTTGAACGTCGGGTGCTATCTGAGACGGCTGATCTGCAGGCCCTGCTGACGCGTGAGCGGGTAG
ATCCAGCACGCCGTGCGGTGTTGCTGCAGCCGCAGGCGATGACCTGGAGCTGGTGGGATGACGCCACCGTCGAGCTGACC
TTCGATCTGCCCGCCGGTAGTTTCGCCACCAGCGTAGTGCGTGAACTGTTCTGTCAGGAAAACTCCGATGCGGATCTTGC
TGAGTAA

Upstream 100 bases:

>100_bases
TGTCAACGTGAAGGCGACCACCACTGAAAAGCTGGGCTTTACCGGGCGTGGGGAGGGGATCGCCTGCGCAGCGGTGGCCC
TGCTGATCAAGGAGTAAACG

Downstream 100 bases:

>100_bases
TGATGATGGTGTAACGGCTCCCGGTATTCAGACGCTGGCAGCGGCGCTACGAGAGTTTGCCCAGGTGCAGGTCGTCGCTC
CGAACCGCAATCGCAGCGGG

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MEFSTLQYLHGRPVATGVVKAFAEDFFVAEDLGFTPDGEGEHVLVRLRKEGCNTQFVAENLARFARIAGRSVSYAGLKDR
HAVTEQWFCLHLPGRDTPDFSQFVLEGCQVLAVARHRKKLRIGTLRGNQFRLVLRHISDSAEVEERLRRVATQGVPNYFG
EQRFGRGGNNLQQARRWATDQIRVKERAKRGFYLSAARSALFNLLVSERLAAGCFSRVLAGDALQLTGRGSWFVAREAEL
SVLQQRLDVGELRITAALPGDGDGGVGGDAAEFERRVLSETADLQALLTRERVDPARRAVLLQPQAMTWSWWDDATVELT
FDLPAGSFATSVVRELFCQENSDADLAE

Sequences:

>Translated_348_residues
MEFSTLQYLHGRPVATGVVKAFAEDFFVAEDLGFTPDGEGEHVLVRLRKEGCNTQFVAENLARFARIAGRSVSYAGLKDR
HAVTEQWFCLHLPGRDTPDFSQFVLEGCQVLAVARHRKKLRIGTLRGNQFRLVLRHISDSAEVEERLRRVATQGVPNYFG
EQRFGRGGNNLQQARRWATDQIRVKERAKRGFYLSAARSALFNLLVSERLAAGCFSRVLAGDALQLTGRGSWFVAREAEL
SVLQQRLDVGELRITAALPGDGDGGVGGDAAEFERRVLSETADLQALLTRERVDPARRAVLLQPQAMTWSWWDDATVELT
FDLPAGSFATSVVRELFCQENSDADLAE
>Mature_348_residues
MEFSTLQYLHGRPVATGVVKAFAEDFFVAEDLGFTPDGEGEHVLVRLRKEGCNTQFVAENLARFARIAGRSVSYAGLKDR
HAVTEQWFCLHLPGRDTPDFSQFVLEGCQVLAVARHRKKLRIGTLRGNQFRLVLRHISDSAEVEERLRRVATQGVPNYFG
EQRFGRGGNNLQQARRWATDQIRVKERAKRGFYLSAARSALFNLLVSERLAAGCFSRVLAGDALQLTGRGSWFVAREAEL
SVLQQRLDVGELRITAALPGDGDGGVGGDAAEFERRVLSETADLQALLTRERVDPARRAVLLQPQAMTWSWWDDATVELT
FDLPAGSFATSVVRELFCQENSDADLAE

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain

Homologues:

Organism=Escherichia coli, GI1789102, Length=348, Percent_Identity=59.1954022988506, Blast_Score=423, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUD_EDWI9 (C5BGI9)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934630.1
- ProteinModelPortal:   C5BGI9
- GeneID:   7961087
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3254
- OMA:   ALTRQWF
- ProtClustDB:   PRK00984
- HAMAP:   MF_01082
- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760
- PANTHER:   PTHR13326
- TIGRFAMs:   TIGR00094

Pfam domain/function: PF01142 TruD; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 38785; Mature: 38785

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS50984 TRUD; PS01268 UPF0024

Important sites: ACT_SITE 79-79 BINDING 26-26 BINDING 128-128 BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFSTLQYLHGRPVATGVVKAFAEDFFVAEDLGFTPDGEGEHVLVRLRKEGCNTQFVAEN
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHH
LARFARIAGRSVSYAGLKDRHAVTEQWFCLHLPGRDTPDFSQFVLEGCQVLAVARHRKKL
HHHHHHHHCCCCCCCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHE
RIGTLRGNQFRLVLRHISDSAEVEERLRRVATQGVPNYFGEQRFGRGGNNLQQARRWATD
EEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHH
QIRVKERAKRGFYLSAARSALFNLLVSERLAAGCFSRVLAGDALQLTGRGSWFVAREAEL
HHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECHHHH
SVLQQRLDVGELRITAALPGDGDGGVGGDAAEFERRVLSETADLQALLTRERVDPARRAV
HHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEE
LLQPQAMTWSWWDDATVELTFDLPAGSFATSVVRELFCQENSDADLAE
EECCCCCEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEFSTLQYLHGRPVATGVVKAFAEDFFVAEDLGFTPDGEGEHVLVRLRKEGCNTQFVAEN
CCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHH
LARFARIAGRSVSYAGLKDRHAVTEQWFCLHLPGRDTPDFSQFVLEGCQVLAVARHRKKL
HHHHHHHHCCCCCCCCCCHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHE
RIGTLRGNQFRLVLRHISDSAEVEERLRRVATQGVPNYFGEQRFGRGGNNLQQARRWATD
EEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHH
QIRVKERAKRGFYLSAARSALFNLLVSERLAAGCFSRVLAGDALQLTGRGSWFVAREAEL
HHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEECHHHH
SVLQQRLDVGELRITAALPGDGDGGVGGDAAEFERRVLSETADLQALLTRERVDPARRAV
HHHHHHCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEE
LLQPQAMTWSWWDDATVELTFDLPAGSFATSVVRELFCQENSDADLAE
EECCCCCEEECCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA