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The map label for this gene is rlmN

Identifier: 238921042

GI number: 238921042

Start: 3054340

End: 3055512

Strand: Reverse

Name: rlmN

Synonym: NT01EI_3172

Alternate gene names: 238921042

Gene position: 3055512-3054340 (Counterclockwise)

Preceding gene: 238921043

Following gene: 238921041

Centisome position: 80.15

GC content: 56.35

Gene sequence:

>1173_bases
TTGTCTGATATTCATGTAACGCCAGTTTTTGCTGACGCCGCGCCGTGTGCTTCTCCGGCACCGGAAAAAATTAACCTGCT
CGATCTGGACCGTAGGCAAATGCGCGAGTTTTTCGTCCAGATGGGCGAAAAGCCGTTTCGCGCCGATCAGATCATGAAGT
GGATCTACCACTATTGCTGTGATGACTTTGACGCGATGACCGACATCAATAAGGTGTTGCGAGCTAGGCTCAAGCAGGTG
GCAGAAATCCGTGCGCCGGAGGTGGCGGTAGAGCAGCGCTCCTCTGACGGTACCATCAAATGGGCGCTGCAGGTGGGCGA
TCAGCGGGTGGAGACGGTCTATATTCCGGAAGACGATCGGGCCACTCTGTGCGTCTCGTCCCAGGTGGGCTGCGCCTTGG
AATGCAAATTCTGTTCGACGGCCCAGCAGGGCTTTAACCGTAACTTGCGGGTATCGGAGATTATCGGTCAGGTATGGCGT
GCGGCAAAAATCATCGGTGCGCAGAAAGTGACCGGTAATCGTCCGATCACCAACGTGGTGATGATGGGCATGGGGGAGCC
GCTGCTCAATCTGACCAACGTGATCCCGGCGATGGAGATTATGCTGGATGATTTTGGCTTTGGCCTCTCCAAGCGTCGCG
TCACTCTGTCGACCTCCGGGGTGGTACCGGCGCTGGATAAGCTGGGCGATACGATCGACGTGGCGCTGGCGATTTCGCTG
CATGCGCCGAACGATACTATCCGCGATGAGATCGTGCCGATTAACCGCAAGTACAATATCGACATGTTTCTGGGATCGGT
TCGCCGCTACCTGGAAAAGTCCAATGCCAATCAGGGGCGAGTGACGGTAGAGTATGTGATGCTGGATCATATTAACGACG
GTACGGAGCACGCGCACCAGCTGGCGGAGTGCCTGAAGGATACGCCTTGCAAGATCAACCTGATCCCGTGGAATCCCTTC
CCGGGAGCGCCCTTTGGCCGCAGCTCCAACAGCCGTATCGATCGTTTCTCCAAGGTGCTGATGGAGTATGGCTTTACCGT
TATCGTGCGTAAGACGCGCGGCGACGATATTGATGCCGCCTGCGGTCAGCTGGCGGGAGAGGTCATCGACAGAACTAAAC
GGACCCTGAAGAAGCAAGCGGCAGGGGAACCTATCAACGTGCGTGAAGTCTGA

Upstream 100 bases:

>100_bases
CGAGCCGCAAAGTGTAACAACGAGGCCTGTTTATTTTATGTCCAAGAATCCTTCATTGCATTCGGGCACTGCGTCCGATG
CCACAGCCATCGAGTCTCCC

Downstream 100 bases:

>100_bases
TGTAGGGACGAACGGCCGCCTGTGACGGCGGCGCAGCGCCAGACACCGGCTGAGCCGCACACGGATGTAGCGGAGCAAGG
GATGAAAACGACGACGGCGC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MSDIHVTPVFADAAPCASPAPEKINLLDLDRRQMREFFVQMGEKPFRADQIMKWIYHYCCDDFDAMTDINKVLRARLKQV
AEIRAPEVAVEQRSSDGTIKWALQVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWR
AAKIIGAQKVTGNRPITNVVMMGMGEPLLNLTNVIPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDTIDVALAISL
HAPNDTIRDEIVPINRKYNIDMFLGSVRRYLEKSNANQGRVTVEYVMLDHINDGTEHAHQLAECLKDTPCKINLIPWNPF
PGAPFGRSSNSRIDRFSKVLMEYGFTVIVRKTRGDDIDAACGQLAGEVIDRTKRTLKKQAAGEPINVREV

Sequences:

>Translated_390_residues
MSDIHVTPVFADAAPCASPAPEKINLLDLDRRQMREFFVQMGEKPFRADQIMKWIYHYCCDDFDAMTDINKVLRARLKQV
AEIRAPEVAVEQRSSDGTIKWALQVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWR
AAKIIGAQKVTGNRPITNVVMMGMGEPLLNLTNVIPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDTIDVALAISL
HAPNDTIRDEIVPINRKYNIDMFLGSVRRYLEKSNANQGRVTVEYVMLDHINDGTEHAHQLAECLKDTPCKINLIPWNPF
PGAPFGRSSNSRIDRFSKVLMEYGFTVIVRKTRGDDIDAACGQLAGEVIDRTKRTLKKQAAGEPINVREV
>Mature_389_residues
SDIHVTPVFADAAPCASPAPEKINLLDLDRRQMREFFVQMGEKPFRADQIMKWIYHYCCDDFDAMTDINKVLRARLKQVA
EIRAPEVAVEQRSSDGTIKWALQVGDQRVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRA
AKIIGAQKVTGNRPITNVVMMGMGEPLLNLTNVIPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDTIDVALAISLH
APNDTIRDEIVPINRKYNIDMFLGSVRRYLEKSNANQGRVTVEYVMLDHINDGTEHAHQLAECLKDTPCKINLIPWNPFP
GAPFGRSSNSRIDRFSKVLMEYGFTVIVRKTRGDDIDAACGQLAGEVIDRTKRTLKKQAAGEPINVREV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=368, Percent_Identity=88.0434782608696, Blast_Score=692, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_EDWI9 (C5BET4)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934557.1
- GeneID:   7958690
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3172
- OMA:   TFCSTAQ
- ProtClustDB:   PRK11194
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 43572; Mature: 43440

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIHVTPVFADAAPCASPAPEKINLLDLDRRQMREFFVQMGEKPFRADQIMKWIYHYCC
CCCCEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
DDFDAMTDINKVLRARLKQVAEIRAPEVAVEQRSSDGTIKWALQVGDQRVETVYIPEDDR
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEEECCCEEEEEEECCCCC
ATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIIGAQKVTGNRPITNVV
EEEEEECCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
MMGMGEPLLNLTNVIPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDTIDVALAISL
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHCCCEEEEEEEEEE
HAPNDTIRDEIVPINRKYNIDMFLGSVRRYLEKSNANQGRVTVEYVMLDHINDGTEHAHQ
ECCCCCHHHCEEECCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHH
LAECLKDTPCKINLIPWNPFPGAPFGRSSNSRIDRFSKVLMEYGFTVIVRKTRGDDIDAA
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHH
CGQLAGEVIDRTKRTLKKQAAGEPINVREV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDIHVTPVFADAAPCASPAPEKINLLDLDRRQMREFFVQMGEKPFRADQIMKWIYHYCC
CCCEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHC
DDFDAMTDINKVLRARLKQVAEIRAPEVAVEQRSSDGTIKWALQVGDQRVETVYIPEDDR
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEEEEEECCCEEEEEEECCCCC
ATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIIGAQKVTGNRPITNVV
EEEEEECCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
MMGMGEPLLNLTNVIPAMEIMLDDFGFGLSKRRVTLSTSGVVPALDKLGDTIDVALAISL
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHCCCEEEEEEEEEE
HAPNDTIRDEIVPINRKYNIDMFLGSVRRYLEKSNANQGRVTVEYVMLDHINDGTEHAHQ
ECCCCCHHHCEEECCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHHH
LAECLKDTPCKINLIPWNPFPGAPFGRSSNSRIDRFSKVLMEYGFTVIVRKTRGDDIDAA
HHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHH
CGQLAGEVIDRTKRTLKKQAAGEPINVREV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA