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The map label for this gene is nrfD [H]

Identifier: 238921029

GI number: 238921029

Start: 3040929

End: 3041870

Strand: Reverse

Name: nrfD [H]

Synonym: NT01EI_3159

Alternate gene names: 238921029

Gene position: 3041870-3040929 (Counterclockwise)

Preceding gene: 238921030

Following gene: 238921028

Centisome position: 79.79

GC content: 65.92

Gene sequence:

>942_bases
ATGACATCGCCGTTTCATTTTCCGTCGCTGGTCTGGGATTGGCCGATCGCCATCGATCTGTTTCTGGTCAGCGTCTCAGC
CGGTCTGGTTGCACTGGCGGTCATGGCGCGCGGCTGGCGCGGAGAGCGCGCGGAGGCCAGCGCGGTGATGCGCGCCACGC
TGTGGCTGGCGCCGCTGACGGTCATGCTGGGGCTGCTGATGCTGGCGCTGCATCTGGCGCGGCCGTGGATGTTCTGGAAG
GTTAGCCTGCACTATAACCTGAGTTCGGTGCTCTCCGTGCGGGTACTGCTGTTCCAGATCTACCTGCCGCTGCTGCTGCT
GTGGCTGGCGCTGTGGTTTACGCCATCGCTGACCGGGCTGCTGGAGCGGAGGTGGCCGGGCGCCCTGCGTCTGGCTGCGC
TGCTGACGCGCCTGCAGGGGCTGCGGCATCCGCTGTCCATCATCCTGCTGATCCTGGCGCTGTTGCTTGGCATCTATAAC
GGCCTGCTGCTCTCGGCGCTGAAGTCTTACCCGATGCTGAACTCGCCCCTGCTGCCCGCGCTGTTCCTGTGCGCCGCGCT
GAGTTGTGGCGTGGCGGTAGCCCTATTGGCCTCGCTGTGGCTGCACGGTGACGACGTGGCGATGGAGCAGGCGCGTCTGC
GCCGTATCGAGGCTCCCCTGGGGGGCGTGTTGTTAGCGCTGCTGCTGCTATTTGTGCTGGGGCTGGCGCAGGGCGATGCG
GCCAAGCAGCGGGCACTGGCCACGCTGCTGGACGGCGGTTTCTGGAGCTGGTGGCTGTGGCTGGGCGTGGTCGGCATTGG
CATTATCCTACCCCTGATGCTGCGCCTGGTGACGCGGGGTAGCCTGCGCCTGCGCATCCTTCTGGGGAGCGGAATAACGC
TATGCGGTCTGCTGCTGCTGCATCTGTTCATCCTGTACGCCGGGCAGCTGACGGTGGTCTGA

Upstream 100 bases:

>100_bases
GCCGTCTGCTGCGCGAACAGCCGAGCTACCGCTACAAGCTGGCGCTGGGCACCCGCCCGAAACTGTATCGTATTCCGTTC
CGCTACGGGGAGGTGAATTC

Downstream 100 bases:

>100_bases
GCTGGCGATGAGGCGAATCTACCCCCTAAGGAGCCGGTGTGCTGTGGTGGCCTGAACTGGGATTTATTGCCCTGCTGCTG
GCGCTGTGCGTCAGCCTGCT

Product: Polysulphide reductase, NrfD

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MTSPFHFPSLVWDWPIAIDLFLVSVSAGLVALAVMARGWRGERAEASAVMRATLWLAPLTVMLGLLMLALHLARPWMFWK
VSLHYNLSSVLSVRVLLFQIYLPLLLLWLALWFTPSLTGLLERRWPGALRLAALLTRLQGLRHPLSIILLILALLLGIYN
GLLLSALKSYPMLNSPLLPALFLCAALSCGVAVALLASLWLHGDDVAMEQARLRRIEAPLGGVLLALLLLFVLGLAQGDA
AKQRALATLLDGGFWSWWLWLGVVGIGIILPLMLRLVTRGSLRLRILLGSGITLCGLLLLHLFILYAGQLTVV

Sequences:

>Translated_313_residues
MTSPFHFPSLVWDWPIAIDLFLVSVSAGLVALAVMARGWRGERAEASAVMRATLWLAPLTVMLGLLMLALHLARPWMFWK
VSLHYNLSSVLSVRVLLFQIYLPLLLLWLALWFTPSLTGLLERRWPGALRLAALLTRLQGLRHPLSIILLILALLLGIYN
GLLLSALKSYPMLNSPLLPALFLCAALSCGVAVALLASLWLHGDDVAMEQARLRRIEAPLGGVLLALLLLFVLGLAQGDA
AKQRALATLLDGGFWSWWLWLGVVGIGIILPLMLRLVTRGSLRLRILLGSGITLCGLLLLHLFILYAGQLTVV
>Mature_312_residues
TSPFHFPSLVWDWPIAIDLFLVSVSAGLVALAVMARGWRGERAEASAVMRATLWLAPLTVMLGLLMLALHLARPWMFWKV
SLHYNLSSVLSVRVLLFQIYLPLLLLWLALWFTPSLTGLLERRWPGALRLAALLTRLQGLRHPLSIILLILALLLGIYNG
LLLSALKSYPMLNSPLLPALFLCAALSCGVAVALLASLWLHGDDVAMEQARLRRIEAPLGGVLLALLLLFVLGLAQGDAA
KQRALATLLDGGFWSWWLWLGVVGIGIILPLMLRLVTRGSLRLRILLGSGITLCGLLLLHLFILYAGQLTVV

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG3301

COG function: function code P; Formate-dependent nitrite reductase, membrane component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI1790509, Length=314, Percent_Identity=48.7261146496815, Blast_Score=260, Evalue=1e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017566
- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 34405; Mature: 34273

Theoretical pI: Translated: 11.04; Mature: 11.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPFHFPSLVWDWPIAIDLFLVSVSAGLVALAVMARGWRGERAEASAVMRATLWLAPLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VMLGLLMLALHLARPWMFWKVSLHYNLSSVLSVRVLLFQIYLPLLLLWLALWFTPSLTGL
HHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERRWPGALRLAALLTRLQGLRHPLSIILLILALLLGIYNGLLLSALKSYPMLNSPLLPA
HHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LFLCAALSCGVAVALLASLWLHGDDVAMEQARLRRIEAPLGGVLLALLLLFVLGLAQGDA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCH
AKQRALATLLDGGFWSWWLWLGVVGIGIILPLMLRLVTRGSLRLRILLGSGITLCGLLLL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
HLFILYAGQLTVV
HHHHHHHCCCCCC
>Mature Secondary Structure 
TSPFHFPSLVWDWPIAIDLFLVSVSAGLVALAVMARGWRGERAEASAVMRATLWLAPLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VMLGLLMLALHLARPWMFWKVSLHYNLSSVLSVRVLLFQIYLPLLLLWLALWFTPSLTGL
HHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LERRWPGALRLAALLTRLQGLRHPLSIILLILALLLGIYNGLLLSALKSYPMLNSPLLPA
HHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LFLCAALSCGVAVALLASLWLHGDDVAMEQARLRRIEAPLGGVLLALLLLFVLGLAQGDA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCH
AKQRALATLLDGGFWSWWLWLGVVGIGIILPLMLRLVTRGSLRLRILLGSGITLCGLLLL
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHH
HLFILYAGQLTVV
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]