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The map label for this gene is nrfE [H]

Identifier: 238921028

GI number: 238921028

Start: 3039196

End: 3040890

Strand: Reverse

Name: nrfE [H]

Synonym: NT01EI_3158

Alternate gene names: 238921028

Gene position: 3040890-3039196 (Counterclockwise)

Preceding gene: 238921029

Following gene: 238921027

Centisome position: 79.76

GC content: 68.38

Gene sequence:

>1695_bases
GTGCTGTGGTGGCCTGAACTGGGATTTATTGCCCTGCTGCTGGCGCTGTGCGTCAGCCTGCTGACGGCGCTGCTGAGCCT
GGCGGGCGGCGGGCCGTGGGCGCAACGCCTGCCCGCGCTGTGTCCACGGCGCTGGTCGTTGGCTCAGCTGGCGCTGACGG
CGGTGGGATTTGTGCTGCTGCTGCTGGCGCTGCTGGCGGATGATTTCTCCCTGCGCTATGTCGCCCAGCACAGCCATTCC
GCGCTGGCGCGCGGGCTAAAGCTGGCCGCCGCCTGGGGCGGCCACGAGGGATCGCTGCTGTTGTGGCTGCTGTGTCTGGC
GGGGTGGAGCGCCCTGTATGCCCTGTGCGCCCGCCGTCAGGCGGCGCAGGCGAACGCCTATACCTTGGGGCTAATGGCGC
TGATCGTCGGCGCCTTTCTGCTGTTTATCGTCACCCTCGGCGATCCCTTCACCCGTCAGTTCCCCGCACCGCCGGAGGGG
CGGGATCTTAACCCGATGCTGCAGCACCCCGGGCTGATCTTCCATCCGCCACTGCTGTACCTGGGCTATGGCGGGCTGGC
CCTGTGCGCCGCCCGTCTGTTGGCTGGGCTGTTGGCGCGCCAGACGGCGCAGGCGATGGCCGATGACTGCTGGCGCTGGC
TGCTGCCGGCTTGGGGGTGCCTGAGCACCGGGATCATCTTGGGGTCATGGTGGGCCTACAGCGAACTGGGCTGGGGCGGG
TTCTGGTTTTGGGATCCGGTGGAAAACGCCTCGCTGTTGCCCTGGCTGAGCGCGACGGCGCTGCTGCACAGCCTGAGCGT
CACCCGGCGCAGCGGCGCGCTGGCGCACTGGTCGCTGCTGCTGGCGCTGACATCGCTGATCTTTACCCTGATGGGCACGC
TGATCGTGCGCTCCGGCGTTTTGCTGTCGGTGCATGCCTTCGCGCTGGACGAGGCGCGCGCGTTGGCGCTATTGCTGCTG
TTCGGCGGTCTTTCCGTCGGTGCCCTGACGCTGTACGCCTTGTCTGCGGGACGGCTGACGCCGGTGACGACGCCGGCCTC
CCTGCCGCTGCAGGGGATCCTGTGGCTGTTCAGCGCCGCGGCACTGATCGTGCTGATCGGCACGCTGTATCCCATGCTGT
ACGGCCTGCTGGGCTGGGGGCGGATTTCGGTGGGCGCGCCTTACTTTAACGGCGTACTGCTGCCATTCGCGCTGCTGGCG
CTGGCGCTGATGCTGTATGGCGGGCTGCGGCGCAGGGTGGGGGCGGGTGGATGCCTGGCCCACTTGGGGGCGCTGCTGTG
TGCGCTGGGGATCGGGCTGCACGCCGCCGGGCAGCAGGAGCGCAGCGTGGCACTGGCGCCCGGGCAGTCGACGCAGCTGG
GGGAGTACCGTTTTACCCTGCGGGAGGTAACGCAGTTTGCGCGCGGTAACTATACCGCCGAGCGCGGGGAGATTTGGGTG
CAGCGGGGGGGGGATACAGTGGCGCGCCTGTATCCGGAGCGACGCCACTATCTGGCCCGCAACCAGCAGATGTTTGAGCC
GGGCATCAGCTGGAGCTGGCGCGATGAATGGTACGCCCTGATGGCGGAAAAGCAGGGCGATCGTTATGCCCTGCGCCTGT
ACCTGCGCCAGGGGGTACGCTGGATTTGGTGCGGCGGCGGTCTGATGATCGCCGGGGCGCTGCTGTGTCTGCGGAGGCGA
CGGAGGGAGCCATGA

Upstream 100 bases:

>100_bases
TATGCGGTCTGCTGCTGCTGCATCTGTTCATCCTGTACGCCGGGCAGCTGACGGTGGTCTGAGCTGGCGATGAGGCGAAT
CTACCCCCTAAGGAGCCGGT

Downstream 100 bases:

>100_bases
GAATCATTAGCGGGGGACTCTGGGCGCTGCTGCTGCTGTGCGGGGCCGCGCGGGCGCAGGTGGTGGATGCGTGGGCGTTT
CCTTCGCCCCAGGCGCGAGA

Product: heme lyase subunit NrfE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 564

Protein sequence:

>564_residues
MLWWPELGFIALLLALCVSLLTALLSLAGGGPWAQRLPALCPRRWSLAQLALTAVGFVLLLLALLADDFSLRYVAQHSHS
ALARGLKLAAAWGGHEGSLLLWLLCLAGWSALYALCARRQAAQANAYTLGLMALIVGAFLLFIVTLGDPFTRQFPAPPEG
RDLNPMLQHPGLIFHPPLLYLGYGGLALCAARLLAGLLARQTAQAMADDCWRWLLPAWGCLSTGIILGSWWAYSELGWGG
FWFWDPVENASLLPWLSATALLHSLSVTRRSGALAHWSLLLALTSLIFTLMGTLIVRSGVLLSVHAFALDEARALALLLL
FGGLSVGALTLYALSAGRLTPVTTPASLPLQGILWLFSAAALIVLIGTLYPMLYGLLGWGRISVGAPYFNGVLLPFALLA
LALMLYGGLRRRVGAGGCLAHLGALLCALGIGLHAAGQQERSVALAPGQSTQLGEYRFTLREVTQFARGNYTAERGEIWV
QRGGDTVARLYPERRHYLARNQQMFEPGISWSWRDEWYALMAEKQGDRYALRLYLRQGVRWIWCGGGLMIAGALLCLRRR
RREP

Sequences:

>Translated_564_residues
MLWWPELGFIALLLALCVSLLTALLSLAGGGPWAQRLPALCPRRWSLAQLALTAVGFVLLLLALLADDFSLRYVAQHSHS
ALARGLKLAAAWGGHEGSLLLWLLCLAGWSALYALCARRQAAQANAYTLGLMALIVGAFLLFIVTLGDPFTRQFPAPPEG
RDLNPMLQHPGLIFHPPLLYLGYGGLALCAARLLAGLLARQTAQAMADDCWRWLLPAWGCLSTGIILGSWWAYSELGWGG
FWFWDPVENASLLPWLSATALLHSLSVTRRSGALAHWSLLLALTSLIFTLMGTLIVRSGVLLSVHAFALDEARALALLLL
FGGLSVGALTLYALSAGRLTPVTTPASLPLQGILWLFSAAALIVLIGTLYPMLYGLLGWGRISVGAPYFNGVLLPFALLA
LALMLYGGLRRRVGAGGCLAHLGALLCALGIGLHAAGQQERSVALAPGQSTQLGEYRFTLREVTQFARGNYTAERGEIWV
QRGGDTVARLYPERRHYLARNQQMFEPGISWSWRDEWYALMAEKQGDRYALRLYLRQGVRWIWCGGGLMIAGALLCLRRR
RREP
>Mature_564_residues
MLWWPELGFIALLLALCVSLLTALLSLAGGGPWAQRLPALCPRRWSLAQLALTAVGFVLLLLALLADDFSLRYVAQHSHS
ALARGLKLAAAWGGHEGSLLLWLLCLAGWSALYALCARRQAAQANAYTLGLMALIVGAFLLFIVTLGDPFTRQFPAPPEG
RDLNPMLQHPGLIFHPPLLYLGYGGLALCAARLLAGLLARQTAQAMADDCWRWLLPAWGCLSTGIILGSWWAYSELGWGG
FWFWDPVENASLLPWLSATALLHSLSVTRRSGALAHWSLLLALTSLIFTLMGTLIVRSGVLLSVHAFALDEARALALLLL
FGGLSVGALTLYALSAGRLTPVTTPASLPLQGILWLFSAAALIVLIGTLYPMLYGLLGWGRISVGAPYFNGVLLPFALLA
LALMLYGGLRRRVGAGGCLAHLGALLCALGIGLHAAGQQERSVALAPGQSTQLGEYRFTLREVTQFARGNYTAERGEIWV
QRGGDTVARLYPERRHYLARNQQMFEPGISWSWRDEWYALMAEKQGDRYALRLYLRQGVRWIWCGGGLMIAGALLCLRRR
RREP

Specific function: May be required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG1138

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family [H]

Homologues:

Organism=Escherichia coli, GI1790511, Length=513, Percent_Identity=55.5555555555556, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI1788524, Length=404, Percent_Identity=43.0693069306931, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002541
- InterPro:   IPR003567
- InterPro:   IPR003568
- InterPro:   IPR003570 [H]

Pfam domain/function: PF01578 Cytochrom_C_asm [H]

EC number: NA

Molecular weight: Translated: 61529; Mature: 61529

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWWPELGFIALLLALCVSLLTALLSLAGGGPWAQRLPALCPRRWSLAQLALTAVGFVLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LLALLADDFSLRYVAQHSHSALARGLKLAAAWGGHEGSLLLWLLCLAGWSALYALCARRQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAQANAYTLGLMALIVGAFLLFIVTLGDPFTRQFPAPPEGRDLNPMLQHPGLIFHPPLLY
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHCCCCEECCHHHH
LGYGGLALCAARLLAGLLARQTAQAMADDCWRWLLPAWGCLSTGIILGSWWAYSELGWGG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
FWFWDPVENASLLPWLSATALLHSLSVTRRSGALAHWSLLLALTSLIFTLMGTLIVRSGV
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
LLSVHAFALDEARALALLLLFGGLSVGALTLYALSAGRLTPVTTPASLPLQGILWLFSAA
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
ALIVLIGTLYPMLYGLLGWGRISVGAPYFNGVLLPFALLALALMLYGGLRRRVGAGGCLA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
HLGALLCALGIGLHAAGQQERSVALAPGQSTQLGEYRFTLREVTQFARGNYTAERGEIWV
HHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
QRGGDTVARLYPERRHYLARNQQMFEPGISWSWRDEWYALMAEKQGDRYALRLYLRQGVR
ECCCCHHHHHCHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCE
WIWCGGGLMIAGALLCLRRRRREP
EEEECCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLWWPELGFIALLLALCVSLLTALLSLAGGGPWAQRLPALCPRRWSLAQLALTAVGFVLL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
LLALLADDFSLRYVAQHSHSALARGLKLAAAWGGHEGSLLLWLLCLAGWSALYALCARRQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAQANAYTLGLMALIVGAFLLFIVTLGDPFTRQFPAPPEGRDLNPMLQHPGLIFHPPLLY
HHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCHHHHCCCCEECCHHHH
LGYGGLALCAARLLAGLLARQTAQAMADDCWRWLLPAWGCLSTGIILGSWWAYSELGWGG
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
FWFWDPVENASLLPWLSATALLHSLSVTRRSGALAHWSLLLALTSLIFTLMGTLIVRSGV
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
LLSVHAFALDEARALALLLLFGGLSVGALTLYALSAGRLTPVTTPASLPLQGILWLFSAA
HHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
ALIVLIGTLYPMLYGLLGWGRISVGAPYFNGVLLPFALLALALMLYGGLRRRVGAGGCLA
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
HLGALLCALGIGLHAAGQQERSVALAPGQSTQLGEYRFTLREVTQFARGNYTAERGEIWV
HHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEE
QRGGDTVARLYPERRHYLARNQQMFEPGISWSWRDEWYALMAEKQGDRYALRLYLRQGVR
ECCCCHHHHHCHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCE
WIWCGGGLMIAGALLCLRRRRREP
EEEECCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]