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The map label for this gene is nrfF [H]

Identifier: 238921027

GI number: 238921027

Start: 3038816

End: 3039199

Strand: Reverse

Name: nrfF [H]

Synonym: NT01EI_3157

Alternate gene names: 238921027

Gene position: 3039199-3038816 (Counterclockwise)

Preceding gene: 238921028

Following gene: 238921026

Centisome position: 79.72

GC content: 65.62

Gene sequence:

>384_bases
ATGAGAATCATTAGCGGGGGACTCTGGGCGCTGCTGCTGCTGTGCGGGGCCGCGCGGGCGCAGGTGGTGGATGCGTGGGC
GTTTCCTTCGCCCCAGGCGCGAGATCAAGGGCTGGCTATCGCCGCCCAGCTACGCTGTCCCCAGTGCCAGAATCAGAACC
TGCTTGAGTCCAATGCGCCGGTGGCCGTGGCGATGCGTCATGAGGTGTTTCGCCGGGTGGCGCAGGGGCAGGATGAGGCG
CAGATCGTCGATTTCATGGCGGCGCGCTACGGAGAGTTTGTGCGCTATCGACCCGCCGTCAATCGCCATACGGTGCTGCT
GTGGGGACTGCCGCCGCTGCTGCTGTTGATGATCGTCGTTGGGCTGTGGCGGAGGCGACGGTGA

Upstream 100 bases:

>100_bases
TGCGCCTGTACCTGCGCCAGGGGGTACGCTGGATTTGGTGCGGCGGCGGTCTGATGATCGCCGGGGCGCTGCTGTGTCTG
CGGAGGCGACGGAGGGAGCC

Downstream 100 bases:

>100_bases
GCCTCTGGCGCGCGCTGCTGCTGCCGGTGCTGGCGCTGAGCCTGGCGCTGGCGGGCTATGGCCTGAGCGGACGGGCGGCG
TCGGTGCTGCAGGCGCAGCG

Product: formate-dependent nitrite reductase complex subunit NrfF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MRIISGGLWALLLLCGAARAQVVDAWAFPSPQARDQGLAIAAQLRCPQCQNQNLLESNAPVAVAMRHEVFRRVAQGQDEA
QIVDFMAARYGEFVRYRPAVNRHTVLLWGLPPLLLLMIVVGLWRRRR

Sequences:

>Translated_127_residues
MRIISGGLWALLLLCGAARAQVVDAWAFPSPQARDQGLAIAAQLRCPQCQNQNLLESNAPVAVAMRHEVFRRVAQGQDEA
QIVDFMAARYGEFVRYRPAVNRHTVLLWGLPPLLLLMIVVGLWRRRR
>Mature_127_residues
MRIISGGLWALLLLCGAARAQVVDAWAFPSPQARDQGLAIAAQLRCPQCQNQNLLESNAPVAVAMRHEVFRRVAQGQDEA
QIVDFMAARYGEFVRYRPAVNRHTVLLWGLPPLLLLMIVVGLWRRRR

Specific function: Not required for the biosynthesis of any of the c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG3088

COG function: function code O; Uncharacterized protein involved in biosynthesis of c-type cytochromes

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CcmH/CycL/Ccl2/NrfF family [H]

Homologues:

Organism=Escherichia coli, GI1790512, Length=87, Percent_Identity=62.0689655172414, Blast_Score=114, Evalue=1e-27,
Organism=Escherichia coli, GI1788522, Length=123, Percent_Identity=37.3983739837398, Blast_Score=89, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005616
- InterPro:   IPR017565 [H]

Pfam domain/function: PF03918 CcmH [H]

EC number: NA

Molecular weight: Translated: 14301; Mature: 14301

Theoretical pI: Translated: 11.40; Mature: 11.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIISGGLWALLLLCGAARAQVVDAWAFPSPQARDQGLAIAAQLRCPQCQNQNLLESNAP
CCEECHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
VAVAMRHEVFRRVAQGQDEAQIVDFMAARYGEFVRYRPAVNRHTVLLWGLPPLLLLMIVV
EEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
GLWRRRR
HHHHCCC
>Mature Secondary Structure
MRIISGGLWALLLLCGAARAQVVDAWAFPSPQARDQGLAIAAQLRCPQCQNQNLLESNAP
CCEECHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
VAVAMRHEVFRRVAQGQDEAQIVDFMAARYGEFVRYRPAVNRHTVLLWGLPPLLLLMIVV
EEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHH
GLWRRRR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]