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The map label for this gene is grpE [H]

Identifier: 238920934

GI number: 238920934

Start: 2957017

End: 2957595

Strand: Reverse

Name: grpE [H]

Synonym: NT01EI_3062

Alternate gene names: 238920934

Gene position: 2957595-2957017 (Counterclockwise)

Preceding gene: 238920938

Following gene: 238920931

Centisome position: 77.58

GC content: 60.1

Gene sequence:

>579_bases
ATGAGCAGTGAACAACAAAACATGACGCAAGAGCCGGCTAACGTAGAACAGGTTGAAAACGAAGCGCTTCAGGCTGAGAC
TCAGGGCGCCGGTGTGGAGCCGGACGCGCGTGATGCCCGCATTGCAGAGCTGGAGGCCCAGCTGAAGGCCATGGGGGAGC
ATGAGCGTGAAATTATGCTGCGCGCCCGTGCCGACGTGGACAACATCCGTCGCCGCGCAGAGCAGGATGTCGAGAAGGCG
CACAAGTTCGCGCTGGAGAAGTTTTCCGGGGAGCTGCTGCCGGTGATCGATAACCTGGAGCGCGCGCTGGAGCTTGCCGA
TAAGTCCAACACTGAGCTGGTATCGATGATCGAAGGGGTGGAGCTGACCCTGAAATCCCTGCTGGACGTCGTGCGTAAGT
TTGGCGTGGAGCAGGTGGCGGAGGTGAACGTACCGTTCAACCCTGAGGTCCACCAGGCGATGACCATGATGGCCTCCCCG
GATCATGCGCCGAACCAGGTGATGATGGTGATGCAGAAAGGCTATACCCTGAACGGTCGCCTGATCCGCCCGGCGATGGT
CGCAGTCTCGAAAGGCTGA

Upstream 100 bases:

>100_bases
TAGAATGGCGCCCATTATGCCCGGCTCTCTTGAATCGCCGACTTTCATCCCCATAATAAGCCAAGTAGCGAGATAAATGC
CAAAAACCGCGGAGAATTCC

Downstream 100 bases:

>100_bases
TTCGGTTCAGAATAGGCGCAAAAACCAGCCCGCGGGCTGGTTTTTTTATGCCTGCGCCTCATCCTGCGGCAGGTGCGGCT
GCCAGTCGATGGGTGCCATC

Product: co-chaperone GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MSSEQQNMTQEPANVEQVENEALQAETQGAGVEPDARDARIAELEAQLKAMGEHEREIMLRARADVDNIRRRAEQDVEKA
HKFALEKFSGELLPVIDNLERALELADKSNTELVSMIEGVELTLKSLLDVVRKFGVEQVAEVNVPFNPEVHQAMTMMASP
DHAPNQVMMVMQKGYTLNGRLIRPAMVAVSKG

Sequences:

>Translated_192_residues
MSSEQQNMTQEPANVEQVENEALQAETQGAGVEPDARDARIAELEAQLKAMGEHEREIMLRARADVDNIRRRAEQDVEKA
HKFALEKFSGELLPVIDNLERALELADKSNTELVSMIEGVELTLKSLLDVVRKFGVEQVAEVNVPFNPEVHQAMTMMASP
DHAPNQVMMVMQKGYTLNGRLIRPAMVAVSKG
>Mature_191_residues
SSEQQNMTQEPANVEQVENEALQAETQGAGVEPDARDARIAELEAQLKAMGEHEREIMLRARADVDNIRRRAEQDVEKAH
KFALEKFSGELLPVIDNLERALELADKSNTELVSMIEGVELTLKSLLDVVRKFGVEQVAEVNVPFNPEVHQAMTMMASPD
HAPNQVMMVMQKGYTLNGRLIRPAMVAVSKG

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=154, Percent_Identity=33.7662337662338, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI40255109, Length=137, Percent_Identity=34.3065693430657, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1788967, Length=195, Percent_Identity=55.3846153846154, Blast_Score=209, Evalue=7e-56,
Organism=Saccharomyces cerevisiae, GI6324806, Length=161, Percent_Identity=35.4037267080745, Blast_Score=105, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24653432, Length=158, Percent_Identity=34.1772151898734, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21378; Mature: 21246

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEQQNMTQEPANVEQVENEALQAETQGAGVEPDARDARIAELEAQLKAMGEHEREIML
CCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHH
RARADVDNIRRRAEQDVEKAHKFALEKFSGELLPVIDNLERALELADKSNTELVSMIEGV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ELTLKSLLDVVRKFGVEQVAEVNVPFNPEVHQAMTMMASPDHAPNQVMMVMQKGYTLNGR
HHHHHHHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCE
LIRPAMVAVSKG
EEHHHHHHCCCC
>Mature Secondary Structure 
SSEQQNMTQEPANVEQVENEALQAETQGAGVEPDARDARIAELEAQLKAMGEHEREIML
CCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHH
RARADVDNIRRRAEQDVEKAHKFALEKFSGELLPVIDNLERALELADKSNTELVSMIEGV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ELTLKSLLDVVRKFGVEQVAEVNVPFNPEVHQAMTMMASPDHAPNQVMMVMQKGYTLNGR
HHHHHHHHHHHHHHCHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCE
LIRPAMVAVSKG
EEHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA