Click here to switch to the map view.

The map label for this gene is grcA

Identifier: 238920931

GI number: 238920931

Start: 2955491

End: 2955874

Strand: Reverse

Name: grcA

Synonym: NT01EI_3059

Alternate gene names: 238920931

Gene position: 2955874-2955491 (Counterclockwise)

Preceding gene: 238920934

Following gene: 238920930

Centisome position: 77.53

GC content: 60.68

Gene sequence:

>384_bases
ATGATTAAAGGTATTCAGATCACCAAGGCTGACAACGCTGACCTGCTGAACTCTTTCTGGCTGCTGGACACAGAGAAAGG
CGCCGCGCGCTGCCTGTGCGCCAAGGGTGGCTACGCTGAGGATCAGATGGTCGCGCTGGACGAGCTGGGCGCATTCGAAT
CCCGTGACGTGGAGCTGGACGTCGCGCCGAGCGTGCGCGTGGAAGGCGGTCAGCACCTGAACGTTAACGTGCTGCGCCGT
GAAACGCTGGAAGACGCCGTGAAGCATCCGGAAAAGTATCCGCAGCTGACCATCCGTGTGTCCGGCTACGCGGTACGCTT
CAACTCCCTGACTCCGGAGCAGCAGCGCGACGTGATCACCCGTACCTTCACCCAGAGCCTGTAA

Upstream 100 bases:

>100_bases
CACATCAAGACAACATTGGTTTTACCAAATGGCCCGCCGGTGGGGCTGGCACCATGACCGAATTGGCCGATTTTTGCTCT
ATCTCAATTGGAGGCAACAA

Downstream 100 bases:

>100_bases
GGGGCGGCGGCACGGGCCGCATGGTGGCGTCAATCAGATAAAAAGGAGCCGACAGGCCTGACGGCTCCCCGGAAACATTA
TCAGTGCACTACGGTGATGT

Product: autonomous glycyl radical cofactor GrcA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 127

Protein sequence:

>127_residues
MIKGIQITKADNADLLNSFWLLDTEKGAARCLCAKGGYAEDQMVALDELGAFESRDVELDVAPSVRVEGGQHLNVNVLRR
ETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTQSL

Sequences:

>Translated_127_residues
MIKGIQITKADNADLLNSFWLLDTEKGAARCLCAKGGYAEDQMVALDELGAFESRDVELDVAPSVRVEGGQHLNVNVLRR
ETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTQSL
>Mature_127_residues
MIKGIQITKADNADLLNSFWLLDTEKGAARCLCAKGGYAEDQMVALDELGAFESRDVELDVAPSVRVEGGQHLNVNVLRR
ETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVITRTFTQSL

Specific function: Acts as a radical domain for damaged PFL and possibly other radical proteins

COG id: COG3445

COG function: function code R; Acid-induced glycyl radical enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glycine radical domain

Homologues:

Organism=Escherichia coli, GI1788933, Length=127, Percent_Identity=81.1023622047244, Blast_Score=218, Evalue=1e-58,
Organism=Escherichia coli, GI1787131, Length=61, Percent_Identity=81.9672131147541, Blast_Score=109, Evalue=6e-26,
Organism=Escherichia coli, GI48994926, Length=61, Percent_Identity=80.327868852459, Blast_Score=107, Evalue=2e-25,
Organism=Escherichia coli, GI1787044, Length=56, Percent_Identity=48.2142857142857, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GRCA_EDWI9 (C5BAK4)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934446.1
- GeneID:   7961830
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3059
- OMA:   GQFEYRE
- ProtClustDB:   PRK11127
- HAMAP:   MF_00806
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR011140
- PIRSF:   PIRSF000378

Pfam domain/function: PF01228 Gly_radical

EC number: NA

Molecular weight: Translated: 14193; Mature: 14193

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00850 GLY_RADICAL_1; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKGIQITKADNADLLNSFWLLDTEKGAARCLCAKGGYAEDQMVALDELGAFESRDVELD
CCCCEEEEECCCCCHHHEEEEEECCCCCEEEEECCCCCCCCCEEEHHHCCCCCCCCEEEE
VAPSVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVIT
CCCCEEECCCCEEEEEHHHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHH
RTFTQSL
HHHHHCC
>Mature Secondary Structure
MIKGIQITKADNADLLNSFWLLDTEKGAARCLCAKGGYAEDQMVALDELGAFESRDVELD
CCCCEEEEECCCCCHHHEEEEEECCCCCEEEEECCCCCCCCCEEEHHHCCCCCCCCEEEE
VAPSVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSGYAVRFNSLTPEQQRDVIT
CCCCEEECCCCEEEEEHHHHHHHHHHHHCCCCCCEEEEEECCEEEEECCCCCHHHHHHHH
RTFTQSL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA