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The map label for this gene is hybO [H]

Identifier: 238920548

GI number: 238920548

Start: 2554580

End: 2555704

Strand: Reverse

Name: hybO [H]

Synonym: NT01EI_2660

Alternate gene names: 238920548

Gene position: 2555704-2554580 (Counterclockwise)

Preceding gene: 238920555

Following gene: 238920547

Centisome position: 67.04

GC content: 61.07

Gene sequence:

>1125_bases
ATGGTTGAGGATTCTTTTTTATCCGCTCACGGCATCAATCGTCGTGACTTTATGAAACTGTGCGCGGGCATGGCCGCCAC
ACTAGGGTTAAGCTCCAATGCGGCGGCGGAAATGGCGGCAGCGATCACCAGTCCGCAGCGTCCTCCGGTTATCTGGATTG
GTGCCCAGGAGTGTACCGGCTGTACCGAGGCCCTGCTGCGTTCCACGCACCCGACGCTGGAAACGCTGCTGCTGAATACC
ATCTCATTGGAATATCATGAGGTACTGTCATCAGCCTTCGGTCATCAGGTTGAAGAAAACCTGCACGACGCCATGGCGCG
CTACAAGGGCAAATATGTCCTGGTGGTGGATGGCTCCATTCCGCTGAAAGACGGCGGCGTGTATTGTATGGTGGCCGGTG
AGCCTATCGTCGATCAGATCCGTAAGGCAGCCGCCGATGCGGCTGCGGTGATCGCTATCGGCTCCTGCTCCGCCTGGGGC
GGCGTTCCGGCCACCGGCGGTAACCCGACCGGCGCCGTCAGCCTGGAAGAGGCGCTGGGTCGTCCGGTGATCAATATCCC
GGGCTGTCCGCCTAACCCGCATAACTTCCTGACCACCGTGGTCTACTACATCACCTATGGCAAGCTGCCGGAGCTGGACA
GCAAGCACCGTCCGCTGTTCGCCTACGAGCGCCTGATCCACGAGAACTGCGAGCGTCGCCCGCACTTCGACGCTGGCCGC
TTCGCCAAAGAGTTCGGTGACTATGGCCACCGTCAGGGCTGGTGTCTGTATCACCTTGGCTGTAAGGGGCCGGAAACCTA
CGGCAACTGCTCGACGCTGGAGTTCTGCGACATCGGCGGCGGCATCTGGCCGGTGGGTATCGGCCACCCATGCTACGGCT
GTAACGAGCAGGGTGTCGGCTTTACCAAGGGGATCTTCCAGCTATCCAGCGTAGAGAATCCGACGCCGCGCGCAGAAAAA
CCGCTGGTGGATAACAAAGAGGGCGGGCATATTTCGCCGACGGCCACCGGCCTTATCGGCGGCGCGCTGGGGATCCTGGC
CGGGGTGAGTCTGATGGCTGTGCGTGAGCTGGGTCGCCAGCAGCAGCGTAACCAGGCGGATCAACACGATTCACGGGAGG
AGTAA

Upstream 100 bases:

>100_bases
AACTTGATGAAGATCAACCACAGCGGGGGGGGCAAATCCTTAGCATTGCGCCCCTGAAAGCATTCCACCAGACGAATGCG
CATAAACAGGAAACTGCAGA

Downstream 100 bases:

>100_bases
ACCGTGAACAGACGAAATTTTCTGAAGTTCGCCTCTGTCGGCGCGCTGGCGGCGGGCACAGCCGCTTCTGGCAGCGCCCA
GGCTGCGGCTGAGAACAAGC

Product: hydrogenase 2 small subunit

Products: NA

Alternate protein names: HYD2; Membrane-bound hydrogenase 2 small subunit; NiFe hydrogenase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MVEDSFLSAHGINRRDFMKLCAGMAATLGLSSNAAAEMAAAITSPQRPPVIWIGAQECTGCTEALLRSTHPTLETLLLNT
ISLEYHEVLSSAFGHQVEENLHDAMARYKGKYVLVVDGSIPLKDGGVYCMVAGEPIVDQIRKAAADAAAVIAIGSCSAWG
GVPATGGNPTGAVSLEEALGRPVINIPGCPPNPHNFLTTVVYYITYGKLPELDSKHRPLFAYERLIHENCERRPHFDAGR
FAKEFGDYGHRQGWCLYHLGCKGPETYGNCSTLEFCDIGGGIWPVGIGHPCYGCNEQGVGFTKGIFQLSSVENPTPRAEK
PLVDNKEGGHISPTATGLIGGALGILAGVSLMAVRELGRQQQRNQADQHDSREE

Sequences:

>Translated_374_residues
MVEDSFLSAHGINRRDFMKLCAGMAATLGLSSNAAAEMAAAITSPQRPPVIWIGAQECTGCTEALLRSTHPTLETLLLNT
ISLEYHEVLSSAFGHQVEENLHDAMARYKGKYVLVVDGSIPLKDGGVYCMVAGEPIVDQIRKAAADAAAVIAIGSCSAWG
GVPATGGNPTGAVSLEEALGRPVINIPGCPPNPHNFLTTVVYYITYGKLPELDSKHRPLFAYERLIHENCERRPHFDAGR
FAKEFGDYGHRQGWCLYHLGCKGPETYGNCSTLEFCDIGGGIWPVGIGHPCYGCNEQGVGFTKGIFQLSSVENPTPRAEK
PLVDNKEGGHISPTATGLIGGALGILAGVSLMAVRELGRQQQRNQADQHDSREE
>Mature_374_residues
MVEDSFLSAHGINRRDFMKLCAGMAATLGLSSNAAAEMAAAITSPQRPPVIWIGAQECTGCTEALLRSTHPTLETLLLNT
ISLEYHEVLSSAFGHQVEENLHDAMARYKGKYVLVVDGSIPLKDGGVYCMVAGEPIVDQIRKAAADAAAVIAIGSCSAWG
GVPATGGNPTGAVSLEEALGRPVINIPGCPPNPHNFLTTVVYYITYGKLPELDSKHRPLFAYERLIHENCERRPHFDAGR
FAKEFGDYGHRQGWCLYHLGCKGPETYGNCSTLEFCDIGGGIWPVGIGHPCYGCNEQGVGFTKGIFQLSSVENPTPRAEK
PLVDNKEGGHISPTATGLIGGALGILAGVSLMAVRELGRQQQRNQADQHDSREE

Specific function: This is one of three E.coli hydrogenases synthesized in response to different physiological conditions. HYD2 is involved in hydrogen uptake [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Periplasmic side. Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=366, Percent_Identity=76.2295081967213, Blast_Score=556, Evalue=1e-160,
Organism=Escherichia coli, GI1787206, Length=316, Percent_Identity=41.1392405063291, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 40116; Mature: 40116

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEDSFLSAHGINRRDFMKLCAGMAATLGLSSNAAAEMAAAITSPQRPPVIWIGAQECTG
CCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECHHHCH
CTEALLRSTHPTLETLLLNTISLEYHEVLSSAFGHQVEENLHDAMARYKGKYVLVVDGSI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
PLKDGGVYCMVAGEPIVDQIRKAAADAAAVIAIGSCSAWGGVPATGGNPTGAVSLEEALG
EECCCCEEEEECCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHC
RPVINIPGCPPNPHNFLTTVVYYITYGKLPELDSKHRPLFAYERLIHENCERRPHFDAGR
CCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
FAKEFGDYGHRQGWCLYHLGCKGPETYGNCSTLEFCDIGGGIWPVGIGHPCYGCNEQGVG
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCEEECCCCCCCCCCCCCCCC
FTKGIFQLSSVENPTPRAEKPLVDNKEGGHISPTATGLIGGALGILAGVSLMAVRELGRQ
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQRNQADQHDSREE
HHHHHHHCCCCCCC
>Mature Secondary Structure
MVEDSFLSAHGINRRDFMKLCAGMAATLGLSSNAAAEMAAAITSPQRPPVIWIGAQECTG
CCCCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEEEEECHHHCH
CTEALLRSTHPTLETLLLNTISLEYHEVLSSAFGHQVEENLHDAMARYKGKYVLVVDGSI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
PLKDGGVYCMVAGEPIVDQIRKAAADAAAVIAIGSCSAWGGVPATGGNPTGAVSLEEALG
EECCCCEEEEECCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCEEHHHHHC
RPVINIPGCPPNPHNFLTTVVYYITYGKLPELDSKHRPLFAYERLIHENCERRPHFDAGR
CCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
FAKEFGDYGHRQGWCLYHLGCKGPETYGNCSTLEFCDIGGGIWPVGIGHPCYGCNEQGVG
HHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCEEEECCCCCEEECCCCCCCCCCCCCCCC
FTKGIFQLSSVENPTPRAEKPLVDNKEGGHISPTATGLIGGALGILAGVSLMAVRELGRQ
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQRNQADQHDSREE
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]