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The map label for this gene is hybA [H]

Identifier: 238920547

GI number: 238920547

Start: 2553593

End: 2554576

Strand: Reverse

Name: hybA [H]

Synonym: NT01EI_2659

Alternate gene names: 238920547

Gene position: 2554576-2553593 (Counterclockwise)

Preceding gene: 238920548

Following gene: 238920546

Centisome position: 67.01

GC content: 57.32

Gene sequence:

>984_bases
GTGAACAGACGAAATTTTCTGAAGTTCGCCTCTGTCGGCGCGCTGGCGGCGGGCACAGCCGCTTCTGGCAGCGCCCAGGC
TGCGGCTGAGAACAAGCCGCCGATCCCGACGGCGGTGGGGATGCTATATGACTCAACGTTGTGCGTGGGTTGCCAGGCCT
GCGTTGCGGAGTGCCAGCGCCTGAACAAAAATCAGCTGAACCCGAACGGCGATCCGACCTGGTCCAACAATGACAAGCTG
ACTCCCTATACCAATAACATTATTCAGGTATGGCGTGATGGTAGCGGGGTCAACAAGGATCAGTTGGAAAACGGCTATGC
CTATATCAAGAAGCAGTGCATGCACTGTGTCGATCCTAACTGCGTTTCCGTCTGCCCGGTATCGGCGCTGAAAAAGCACC
CCGTCACCGGGATCGTCATGTACGATCCTGACGTCTGCACCGGCTGTCGCTATTGCATGGTGGCCTGCCCGTTCGACGTA
CCGAAGTATGATTACGATAACCCGTTCGGGGCGATTCACAAGTGTGAGCTGTGTAATCAGAAAGGGTTGGAGCGTATCGA
TCAGGGTAAGCTGCCGGGTTGTGTCGAGGTGTGTCCTGCCGGTGCAGTGATCTATGGTACCCGTGACGCGCTGCTGGCCG
AGGCCAAGAAGCGTCTGGCAGCGCGTCCCGGTGCAGAGTATGCCTATCCGCGTCAGACCCTGCACAGTAACGACAGCTAT
CTGCATCCGGTCGCCCACTATCAGCCTTATGTGTATGGCGAGAAAGAGGGGGGAGGGACCCAGGTTCTGGTTCTGGCGGG
CGTACCCTACGAGAAGCTGGGGATGCCGCAGTTGGATGCGCTGTCGACGGGCGCCCGTTCCGAGCATATTCAGCATACGC
TGTACAAAGGCATGATCCTGCCGTTGGTGGTCCTGGCCGGTCTTTCGGTAATGACCTATCGAAACACCAAGAATCACCAT
GACGGAGACGATGATAATGAGTGA

Upstream 100 bases:

>100_bases
GGGGATCCTGGCCGGGGTGAGTCTGATGGCTGTGCGTGAGCTGGGTCGCCAGCAGCAGCGTAACCAGGCGGATCAACACG
ATTCACGGGAGGAGTAAACC

Downstream 100 bases:

>100_bases
GCATAAAGCCAGTCCGCTCGGCGGGCGTCTGGTCAGTTGGCCCGTCGTGTTGCTGGCCCCGTTCATCCTGCTGTGCGGGA
TGTTTATCCTGAAACGTTTG

Product: hydrogenase 2 protein HybA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNRRNFLKFASVGALAAGTAASGSAQAAAENKPPIPTAVGMLYDSTLCVGCQACVAECQRLNKNQLNPNGDPTWSNNDKL
TPYTNNIIQVWRDGSGVNKDQLENGYAYIKKQCMHCVDPNCVSVCPVSALKKHPVTGIVMYDPDVCTGCRYCMVACPFDV
PKYDYDNPFGAIHKCELCNQKGLERIDQGKLPGCVEVCPAGAVIYGTRDALLAEAKKRLAARPGAEYAYPRQTLHSNDSY
LHPVAHYQPYVYGEKEGGGTQVLVLAGVPYEKLGMPQLDALSTGARSEHIQHTLYKGMILPLVVLAGLSVMTYRNTKNHH
DGDDDNE

Sequences:

>Translated_327_residues
MNRRNFLKFASVGALAAGTAASGSAQAAAENKPPIPTAVGMLYDSTLCVGCQACVAECQRLNKNQLNPNGDPTWSNNDKL
TPYTNNIIQVWRDGSGVNKDQLENGYAYIKKQCMHCVDPNCVSVCPVSALKKHPVTGIVMYDPDVCTGCRYCMVACPFDV
PKYDYDNPFGAIHKCELCNQKGLERIDQGKLPGCVEVCPAGAVIYGTRDALLAEAKKRLAARPGAEYAYPRQTLHSNDSY
LHPVAHYQPYVYGEKEGGGTQVLVLAGVPYEKLGMPQLDALSTGARSEHIQHTLYKGMILPLVVLAGLSVMTYRNTKNHH
DGDDDNE
>Mature_327_residues
MNRRNFLKFASVGALAAGTAASGSAQAAAENKPPIPTAVGMLYDSTLCVGCQACVAECQRLNKNQLNPNGDPTWSNNDKL
TPYTNNIIQVWRDGSGVNKDQLENGYAYIKKQCMHCVDPNCVSVCPVSALKKHPVTGIVMYDPDVCTGCRYCMVACPFDV
PKYDYDNPFGAIHKCELCNQKGLERIDQGKLPGCVEVCPAGAVIYGTRDALLAEAKKRLAARPGAEYAYPRQTLHSNDSY
LHPVAHYQPYVYGEKEGGGTQVLVLAGVPYEKLGMPQLDALSTGARSEHIQHTLYKGMILPLVVLAGLSVMTYRNTKNHH
DGDDDNE

Specific function: Participates in the periplasmic electron-transferring activity of hydrogenase 2 during its catalytic turnover [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=328, Percent_Identity=76.8292682926829, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI2367345, Length=254, Percent_Identity=31.496062992126, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790326, Length=183, Percent_Identity=32.2404371584699, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787749, Length=298, Percent_Identity=27.1812080536913, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787122, Length=216, Percent_Identity=31.9444444444444, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787872, Length=216, Percent_Identity=31.4814814814815, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI226510944, Length=236, Percent_Identity=27.5423728813559, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787478, Length=144, Percent_Identity=32.6388888888889, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787740, Length=99, Percent_Identity=35.3535353535354, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 35589; Mature: 35589

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
4.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRNFLKFASVGALAAGTAASGSAQAAAENKPPIPTAVGMLYDSTLCVGCQACVAECQR
CCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LNKNQLNPNGDPTWSNNDKLTPYTNNIIQVWRDGSGVNKDQLENGYAYIKKQCMHCVDPN
HHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCC
CVSVCPVSALKKHPVTGIVMYDPDVCTGCRYCMVACPFDVPKYDYDNPFGAIHKCELCNQ
CCEECCHHHHHCCCCEEEEEECCCHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
KGLERIDQGKLPGCVEVCPAGAVIYGTRDALLAEAKKRLAARPGAEYAYPRQTLHSNDSY
HHHHHHHCCCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCE
LHPVAHYQPYVYGEKEGGGTQVLVLAGVPYEKLGMPQLDALSTGARSEHIQHTLYKGMIL
ECCHHHCCCEEEECCCCCCEEEEEEECCCHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHH
PLVVLAGLSVMTYRNTKNHHDGDDDNE
HHHHHHCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MNRRNFLKFASVGALAAGTAASGSAQAAAENKPPIPTAVGMLYDSTLCVGCQACVAECQR
CCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LNKNQLNPNGDPTWSNNDKLTPYTNNIIQVWRDGSGVNKDQLENGYAYIKKQCMHCVDPN
HHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCC
CVSVCPVSALKKHPVTGIVMYDPDVCTGCRYCMVACPFDVPKYDYDNPFGAIHKCELCNQ
CCEECCHHHHHCCCCEEEEEECCCHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHH
KGLERIDQGKLPGCVEVCPAGAVIYGTRDALLAEAKKRLAARPGAEYAYPRQTLHSNDSY
HHHHHHHCCCCCCHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCE
LHPVAHYQPYVYGEKEGGGTQVLVLAGVPYEKLGMPQLDALSTGARSEHIQHTLYKGMIL
ECCHHHCCCEEEECCCCCCEEEEEEECCCHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHH
PLVVLAGLSVMTYRNTKNHHDGDDDNE
HHHHHHCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]