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The map label for this gene is menC

Identifier: 238920535

GI number: 238920535

Start: 2541362

End: 2542333

Strand: Reverse

Name: menC

Synonym: NT01EI_2647

Alternate gene names: 238920535

Gene position: 2542333-2541362 (Counterclockwise)

Preceding gene: 238920536

Following gene: 238920534

Centisome position: 66.69

GC content: 65.84

Gene sequence:

>972_bases
ATGCGTCAGGCCACGTTGTATCGCTATCGTCTGCCGGTGGACGCCGGTGTCGCGCTGCGTAACCAGCGGCTGAAAAGCCG
CGATGGGCTACTGGTGCGGCTGCACGAGGACGGACGCGAAGGGTGGGGCGAGATAGCCCCGTTACCGGAGTTTAGCCATG
AAACGCTGCCTGAAGCTCAGGAGGCCGCGTGCCGCCTGTTGGCGCAGTGGCAGGTGGGCGCGGCGCCGGCGGAGAGCCCG
CTGCCATCGGTTGCCTTTGGCCTGAGTTGTGCGCAGGCGGAGCTTTCCGGTACGCTGCCGCAGGCAGCCAACTACCGATC
GGCGACGCTGTGCAACGGCGATCCCGATGCGCTGTTTCAGGTGCTGCAGTCGATCCCCGGCGAGAAAGTGGCCAAGGTGA
AGGTCGGGCTGTATGAGGCGGTGCGCGACGGAATGATCGTCAATCTACTGCTGGAGGCGCTGCCGGATCTGCATCTGCGT
CTGGATGCCAACCGTAGCTGGACCCGGGCCAAGGCGGATGGTTTTGCCAAGTATGTCAATCCGGACTGGCGCGACCGTAT
TCTGTTTCTGGAAGAGCCGTGCAAGACCCGCGATGAGTCGCGCGCCTTTGCCCGCGACACCGGGATCGCCATCGCCTGGG
ATGAGAGCGTGCGCGATGCGGACTTCCGCGTCGAGGCGGAGCCGGGCGTGGCGGCCATCATCATCAAGCCGACCCTGACC
GGCAGCCTGCAGCGCTGTCGGCAGCTGGTGACACAGGCGCACCAGGCGGGTTTGAGTGCAGTGATCAGCTCCAGTATTGA
ATCCAGCCTGGGGCTGAGTCAGCTGGCGCGCATGGCGGCCTGGCTGACGCCGGGCACGCCGCCGGGTCTGGATACCCTGA
TGCTGATGCAGGCGCAGCTGCTGCGGGCATGGCCGGCGTGTACGCTGCCGCTGTGGGGTGAGGATACGCTGGACGTGGTA
TGGCAGGGGTGA

Upstream 100 bases:

>100_bases
CCACCATGCTGTTCTACATGACGGAAGAGGGGCAGGAAGGGCGCAACGCATTTAACGAAAAGCGTCAGCCGGACTTTTCC
AAGTTTAAGCGGAATCCGTA

Downstream 100 bases:

>100_bases
GGTGCTTTGACGACTGGCCCTGGCGTCACTGGGCGCATCAGCGTCCGGCCGATATCGCGCTGCGCAGCGACGGCGAGGAG
ATTGACTGGCAGACGCTGGC

Product: O-succinylbenzoate synthase

Products: NA

Alternate protein names: OSB synthase; OSBS; 4-(2'-carboxyphenyl)-4-oxybutyric acid synthase; o-succinylbenzoic acid synthase

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MRQATLYRYRLPVDAGVALRNQRLKSRDGLLVRLHEDGREGWGEIAPLPEFSHETLPEAQEAACRLLAQWQVGAAPAESP
LPSVAFGLSCAQAELSGTLPQAANYRSATLCNGDPDALFQVLQSIPGEKVAKVKVGLYEAVRDGMIVNLLLEALPDLHLR
LDANRSWTRAKADGFAKYVNPDWRDRILFLEEPCKTRDESRAFARDTGIAIAWDESVRDADFRVEAEPGVAAIIIKPTLT
GSLQRCRQLVTQAHQAGLSAVISSSIESSLGLSQLARMAAWLTPGTPPGLDTLMLMQAQLLRAWPACTLPLWGEDTLDVV
WQG

Sequences:

>Translated_323_residues
MRQATLYRYRLPVDAGVALRNQRLKSRDGLLVRLHEDGREGWGEIAPLPEFSHETLPEAQEAACRLLAQWQVGAAPAESP
LPSVAFGLSCAQAELSGTLPQAANYRSATLCNGDPDALFQVLQSIPGEKVAKVKVGLYEAVRDGMIVNLLLEALPDLHLR
LDANRSWTRAKADGFAKYVNPDWRDRILFLEEPCKTRDESRAFARDTGIAIAWDESVRDADFRVEAEPGVAAIIIKPTLT
GSLQRCRQLVTQAHQAGLSAVISSSIESSLGLSQLARMAAWLTPGTPPGLDTLMLMQAQLLRAWPACTLPLWGEDTLDVV
WQG
>Mature_323_residues
MRQATLYRYRLPVDAGVALRNQRLKSRDGLLVRLHEDGREGWGEIAPLPEFSHETLPEAQEAACRLLAQWQVGAAPAESP
LPSVAFGLSCAQAELSGTLPQAANYRSATLCNGDPDALFQVLQSIPGEKVAKVKVGLYEAVRDGMIVNLLLEALPDLHLR
LDANRSWTRAKADGFAKYVNPDWRDRILFLEEPCKTRDESRAFARDTGIAIAWDESVRDADFRVEAEPGVAAIIIKPTLT
GSLQRCRQLVTQAHQAGLSAVISSSIESSLGLSQLARMAAWLTPGTPPGLDTLMLMQAQLLRAWPACTLPLWGEDTLDVV
WQG

Specific function: Converts SHCHC to OSB

COG id: COG1441

COG function: function code H; O-succinylbenzoate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. MenC type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1788596, Length=319, Percent_Identity=68.6520376175549, Blast_Score=426, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENC_EDWI9 (C5B7H9)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934050.1
- ProteinModelPortal:   C5B7H9
- GeneID:   7961673
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_2647
- OMA:   YEANRDG
- ProtClustDB:   PRK05105
- HAMAP:   MF_00470
- InterPro:   IPR013342
- InterPro:   IPR010196
- SMART:   SM00922
- TIGRFAMs:   TIGR01927

Pfam domain/function: PF01188 MR_MLE

EC number: =4.2.1.113

Molecular weight: Translated: 35387; Mature: 35387

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: ACT_SITE 134-134 ACT_SITE 236-236

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQATLYRYRLPVDAGVALRNQRLKSRDGLLVRLHEDGREGWGEIAPLPEFSHETLPEAQ
CCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHH
EAACRLLAQWQVGAAPAESPLPSVAFGLSCAQAELSGTLPQAANYRSATLCNGDPDALFQ
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCHHHHHH
VLQSIPGEKVAKVKVGLYEAVRDGMIVNLLLEALPDLHLRLDANRSWTRAKADGFAKYVN
HHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCHHHHCCHHHHCC
PDWRDRILFLEEPCKTRDESRAFARDTGIAIAWDESVRDADFRVEAEPGVAAIIIKPTLT
CCCCCEEEEECCCCCCCHHHHHHHHHCCEEEEECCCCCCCCEEEECCCCEEEEEECCCCC
GSLQRCRQLVTQAHQAGLSAVISSSIESSLGLSQLARMAAWLTPGTPPGLDTLMLMQAQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LRAWPACTLPLWGEDTLDVVWQG
HHHCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MRQATLYRYRLPVDAGVALRNQRLKSRDGLLVRLHEDGREGWGEIAPLPEFSHETLPEAQ
CCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHH
EAACRLLAQWQVGAAPAESPLPSVAFGLSCAQAELSGTLPQAANYRSATLCNGDPDALFQ
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCHHHHHH
VLQSIPGEKVAKVKVGLYEAVRDGMIVNLLLEALPDLHLRLDANRSWTRAKADGFAKYVN
HHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCCCCCHHHHCCHHHHCC
PDWRDRILFLEEPCKTRDESRAFARDTGIAIAWDESVRDADFRVEAEPGVAAIIIKPTLT
CCCCCEEEEECCCCCCCHHHHHHHHHCCEEEEECCCCCCCCEEEECCCCEEEEEECCCCC
GSLQRCRQLVTQAHQAGLSAVISSSIESSLGLSQLARMAAWLTPGTPPGLDTLMLMQAQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LRAWPACTLPLWGEDTLDVVWQG
HHHCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA