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The map label for this gene is menE [H]

Identifier: 238920534

GI number: 238920534

Start: 2539974

End: 2541365

Strand: Reverse

Name: menE [H]

Synonym: NT01EI_2646

Alternate gene names: 238920534

Gene position: 2541365-2539974 (Counterclockwise)

Preceding gene: 238920535

Following gene: 238920533

Centisome position: 66.66

GC content: 68.1

Gene sequence:

>1392_bases
GTGAGGTGCTTTGACGACTGGCCCTGGCGTCACTGGGCGCATCAGCGTCCGGCCGATATCGCGCTGCGCAGCGACGGCGA
GGAGATTGACTGGCAGACGCTGGCGCAGCGTATTGATGGGCTGGCCGACGGGCTATGCGTACAGGGCGTCGTCTCCGGTG
CCGTAGTTGCTTTGCGGGCGAAAAATGGCCCCGAGGCGCTGTATGCCTATCTGGCGTTGCTGCAGCTGGGCGCCCGGGTG
CTGCCGCTGAACCCACAGCTGCCCGCCGCGCTGCTCGACGTCTTGCTGCCGCGGCTCAATGTGGCCTTTATGCTGGATTT
GGCGCCCGGGCCTGCGCTGAGCTGGCCCGAGGGGATTCAGACGCTGTATTTACGTTCGGGGGCGGCCGCGGCGTCTGCCC
CATGGGCGTCCCGGCGCCAGGCGACGCTGACGTTGACCTCGGGGTCCAGTGGGTTGCCTAAGGCGGCGGTGCATACGGCT
GCAGCGCATCTGGCCTCGGCCCGGGGCGTGATCTCCCTGATGCCACTGGCGGCGCAGGATACCTGGCTGCTGTCGCTGCC
GCTGTACCATGTCTCCGGACAGGGGATCGTCTGGCGCTGGCTGCTCGCCGGTGCCCGCCTGGCGCTGCGTGCGATGCACC
CGCTGGAACGGGCGCTGGACGGCTGTAGCCATGCGTCGCTGGTGCCGACCCAGCTGTGGCGTCTGCTGCAGTCGCCGCAG
CCGGTGGCCCTGCGCGACGTTTTGCTGGGAGGGGCGATGATCCCGCTGGCGCTAACCCAGGCGGCGGAGGCGCGCGGTAT
TCGCTGTTGGTGCGGCTATGGTCTGACGGAGATGGCCTCCACGGTGTGCGCCAAGCGTGCGGATGCCCTGCCGGGCGTGG
GAACGGCCCTGTCGCAGCGTGAGGTTTGCCTGGCGGCGGACGGCGAAGTCCTGCTGCGCGGCGACAGCATGGCCTGCGGT
TACTGGCAGGATGGACGTCTGCACCCGCTGCTGGGTGACGACGGCTGGTTCCATACCCGCGATCGCGGGGCGTGGGCGTC
CGGCGAGCTGCGCATACTGGGGCGGTTGGATAACCAGTTTTTCAGCGGGGGTGAGGGGATCCAGCCGGAGGATATCGAGC
GTATTCTGCAGCAGCATCCGGCGGTTTCTCAGGCCTTTATCGTGCCGGTGGATGACGCCGAGTTTGGCCAGCGTCCGCTG
GCGGTGGTGGAGTGCAGCGTGGCGCCGGCGTCGCTGGCCGCCTGGTTGGAAAGCCGGGTAGCGCGCTTTCAGCGTCCGGT
GGCCTGGCTTTTGCTGCCCGAAACGCTGAAAAGTGGCGGGATTAAAATCGCTCGCCGTCAGGTGCAGCAGTGGGCCGTGC
AGGCCTACCGCGCGCAGGTGGATCTGACCTGA

Upstream 100 bases:

>100_bases
TGGATACCCTGATGCTGATGCAGGCGCAGCTGCTGCGGGCATGGCCGGCGTGTACGCTGCCGCTGTGGGGTGAGGATACG
CTGGACGTGGTATGGCAGGG

Downstream 100 bases:

>100_bases
CGGTACCCGGTGTCGCCCGCTTGCCGTACGGCGACGTCGGGATAATCTTAAATAGTGCGCCCGGCCTGGCAACGGGGCGC
TTTTTTTATTCTGTTTCTCC

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MRCFDDWPWRHWAHQRPADIALRSDGEEIDWQTLAQRIDGLADGLCVQGVVSGAVVALRAKNGPEALYAYLALLQLGARV
LPLNPQLPAALLDVLLPRLNVAFMLDLAPGPALSWPEGIQTLYLRSGAAAASAPWASRRQATLTLTSGSSGLPKAAVHTA
AAHLASARGVISLMPLAAQDTWLLSLPLYHVSGQGIVWRWLLAGARLALRAMHPLERALDGCSHASLVPTQLWRLLQSPQ
PVALRDVLLGGAMIPLALTQAAEARGIRCWCGYGLTEMASTVCAKRADALPGVGTALSQREVCLAADGEVLLRGDSMACG
YWQDGRLHPLLGDDGWFHTRDRGAWASGELRILGRLDNQFFSGGEGIQPEDIERILQQHPAVSQAFIVPVDDAEFGQRPL
AVVECSVAPASLAAWLESRVARFQRPVAWLLLPETLKSGGIKIARRQVQQWAVQAYRAQVDLT

Sequences:

>Translated_463_residues
MRCFDDWPWRHWAHQRPADIALRSDGEEIDWQTLAQRIDGLADGLCVQGVVSGAVVALRAKNGPEALYAYLALLQLGARV
LPLNPQLPAALLDVLLPRLNVAFMLDLAPGPALSWPEGIQTLYLRSGAAAASAPWASRRQATLTLTSGSSGLPKAAVHTA
AAHLASARGVISLMPLAAQDTWLLSLPLYHVSGQGIVWRWLLAGARLALRAMHPLERALDGCSHASLVPTQLWRLLQSPQ
PVALRDVLLGGAMIPLALTQAAEARGIRCWCGYGLTEMASTVCAKRADALPGVGTALSQREVCLAADGEVLLRGDSMACG
YWQDGRLHPLLGDDGWFHTRDRGAWASGELRILGRLDNQFFSGGEGIQPEDIERILQQHPAVSQAFIVPVDDAEFGQRPL
AVVECSVAPASLAAWLESRVARFQRPVAWLLLPETLKSGGIKIARRQVQQWAVQAYRAQVDLT
>Mature_463_residues
MRCFDDWPWRHWAHQRPADIALRSDGEEIDWQTLAQRIDGLADGLCVQGVVSGAVVALRAKNGPEALYAYLALLQLGARV
LPLNPQLPAALLDVLLPRLNVAFMLDLAPGPALSWPEGIQTLYLRSGAAAASAPWASRRQATLTLTSGSSGLPKAAVHTA
AAHLASARGVISLMPLAAQDTWLLSLPLYHVSGQGIVWRWLLAGARLALRAMHPLERALDGCSHASLVPTQLWRLLQSPQ
PVALRDVLLGGAMIPLALTQAAEARGIRCWCGYGLTEMASTVCAKRADALPGVGTALSQREVCLAADGEVLLRGDSMACG
YWQDGRLHPLLGDDGWFHTRDRGAWASGELRILGRLDNQFFSGGEGIQPEDIERILQQHPAVSQAFIVPVDDAEFGQRPL
AVVECSVAPASLAAWLESRVARFQRPVAWLLLPETLKSGGIKIARRQVQQWAVQAYRAQVDLT

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=448, Percent_Identity=57.3660714285714, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI145693145, Length=487, Percent_Identity=26.8993839835729, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI1788107, Length=355, Percent_Identity=26.4788732394366, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=452, Percent_Identity=25, Blast_Score=95, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=438, Percent_Identity=22.6027397260274, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=336, Percent_Identity=25, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=336, Percent_Identity=25, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=336, Percent_Identity=25, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=368, Percent_Identity=23.9130434782609, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=355, Percent_Identity=23.943661971831, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24581924, Length=360, Percent_Identity=25.5555555555556, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50095; Mature: 50095

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCFDDWPWRHWAHQRPADIALRSDGEEIDWQTLAQRIDGLADGLCVQGVVSGAVVALRA
CCCCCCCCHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCEEEEEEC
KNGPEALYAYLALLQLGARVLPLNPQLPAALLDVLLPRLNVAFMLDLAPGPALSWPEGIQ
CCCHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHH
TLYLRSGAAAASAPWASRRQATLTLTSGSSGLPKAAVHTAAAHLASARGVISLMPLAAQD
EEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEEECCCCCC
TWLLSLPLYHVSGQGIVWRWLLAGARLALRAMHPLERALDGCSHASLVPTQLWRLLQSPQ
CEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
PVALRDVLLGGAMIPLALTQAAEARGIRCWCGYGLTEMASTVCAKRADALPGVGTALSQR
CHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHCCC
EVCLAADGEVLLRGDSMACGYWQDGRLHPLLGDDGWFHTRDRGAWASGELRILGRLDNQF
CEEEECCCCEEEECCCEEECCCCCCCCCEEECCCCCEEECCCCCCCCCCEEEEEECCHHH
FSGGEGIQPEDIERILQQHPAVSQAFIVPVDDAEFGQRPLAVVECSVAPASLAAWLESRV
HCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHCCCCEEEEEECCCHHHHHHHHHHHH
ARFQRPVAWLLLPETLKSGGIKIARRQVQQWAVQAYRAQVDLT
HHHHCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRCFDDWPWRHWAHQRPADIALRSDGEEIDWQTLAQRIDGLADGLCVQGVVSGAVVALRA
CCCCCCCCHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHCCEEEEEEC
KNGPEALYAYLALLQLGARVLPLNPQLPAALLDVLLPRLNVAFMLDLAPGPALSWPEGIQ
CCCHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHH
TLYLRSGAAAASAPWASRRQATLTLTSGSSGLPKAAVHTAAAHLASARGVISLMPLAAQD
EEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEEECCCCCC
TWLLSLPLYHVSGQGIVWRWLLAGARLALRAMHPLERALDGCSHASLVPTQLWRLLQSPQ
CEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
PVALRDVLLGGAMIPLALTQAAEARGIRCWCGYGLTEMASTVCAKRADALPGVGTALSQR
CHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCHHCCC
EVCLAADGEVLLRGDSMACGYWQDGRLHPLLGDDGWFHTRDRGAWASGELRILGRLDNQF
CEEEECCCCEEEECCCEEECCCCCCCCCEEECCCCCEEECCCCCCCCCCEEEEEECCHHH
FSGGEGIQPEDIERILQQHPAVSQAFIVPVDDAEFGQRPLAVVECSVAPASLAAWLESRV
HCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHCCCCEEEEEECCCHHHHHHHHHHHH
ARFQRPVAWLLLPETLKSGGIKIARRQVQQWAVQAYRAQVDLT
HHHHCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]