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The map label for this gene is hisF

Identifier: 238920459

GI number: 238920459

Start: 2461900

End: 2462676

Strand: Direct

Name: hisF

Synonym: NT01EI_2570

Alternate gene names: 238920459

Gene position: 2461900-2462676 (Clockwise)

Preceding gene: 238920458

Following gene: 238920460

Centisome position: 64.58

GC content: 64.22

Gene sequence:

>777_bases
ATGCTGGCAAAACGCATAATTCCCTGTCTGGACGTTCGCGACGGCCAGGTCGTCAAAGGCGTTCAGTTTCGCCATCACGA
GATCATCGGCGATATCGTTCCGCTGGCCCGGCGCTACGCCGATGAGGGTGCCGATGAGCTGGTGTTCTATGACATTACCG
CCTCTGCCGACGGCCGTGTGGTCGACAAAAGCTGGGTGGCCCGGGTCGCCGCGGCGATCGATATTCCCTTCTGCGTCGCC
GGTGGGATCCGCAGCGTCAAGGAGGCTGGGCTGCTCCTGAGCTACGGCGCGGACAAGATCTCCATTAACTCGCCGGCGCT
GGCCGAGCCGACGCTGATCACCCGTCTGGCGGATCGCTTCGGCGTGCAGTGTGTGGTGGTCGGTATCGATACCTGGCACG
ACGCCGCCAGCGACAGCGACCGGGTCTACCAGTTCACCGGCGATGAGGCGCGCACCCGCGCCACCGCCTGGCAGACGGCG
GACTGGGTGCAGGAGGTGCAGCGCCGCGGCGCCGGGGAGATCGTGCTGAACATGATGAATCAGGACGGCGTACGCAGCGG
CTACGATCTGCGCCAGCTAGAGCAGATCCGTGCCGTCTGCCGCGTCCCGCTGATCGCCTCCGGCGGTGCCGGTGCCCCCG
AACACTTTCTGGCGGCCTTCAGCCAGGCCGACGTGGACGGCGCGCTGGCGGCCTCGGTATTTCATAAACAGATTATTCAA
ATCGGTGAACTCAAGCGCTATCTGGCGCTCAACGGAGTGGAGATTCGATTGTGCTGA

Upstream 100 bases:

>100_bases
CGCTGCAGGACATTACTGCCCTGCGCGGCAGCGGCGTGCAGGGCATCATCGTCGGCCGTGCCCTGCTGGAAGGCAAATTC
AGCGTGAAGGAGGCCATCGC

Downstream 100 bases:

>100_bases
CTCAAGGTTTTCTTTCGCAACAGCAAAAAGAGCGTCTGGACTGGAGCAAAGTGGACGATATGATGCCGGCGATCGTGCAG
CACGCCGGCTCTGGCGATGT

Product: imidazoleglycerol phosphate synthase, cyclase subunit

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVRDGQVVKGVQFRHHEIIGDIVPLARRYADEGADELVFYDITASADGRVVDKSWVARVAAAIDIPFCVA
GGIRSVKEAGLLLSYGADKISINSPALAEPTLITRLADRFGVQCVVVGIDTWHDAASDSDRVYQFTGDEARTRATAWQTA
DWVQEVQRRGAGEIVLNMMNQDGVRSGYDLRQLEQIRAVCRVPLIASGGAGAPEHFLAAFSQADVDGALAASVFHKQIIQ
IGELKRYLALNGVEIRLC

Sequences:

>Translated_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRHHEIIGDIVPLARRYADEGADELVFYDITASADGRVVDKSWVARVAAAIDIPFCVA
GGIRSVKEAGLLLSYGADKISINSPALAEPTLITRLADRFGVQCVVVGIDTWHDAASDSDRVYQFTGDEARTRATAWQTA
DWVQEVQRRGAGEIVLNMMNQDGVRSGYDLRQLEQIRAVCRVPLIASGGAGAPEHFLAAFSQADVDGALAASVFHKQIIQ
IGELKRYLALNGVEIRLC
>Mature_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRHHEIIGDIVPLARRYADEGADELVFYDITASADGRVVDKSWVARVAAAIDIPFCVA
GGIRSVKEAGLLLSYGADKISINSPALAEPTLITRLADRFGVQCVVVGIDTWHDAASDSDRVYQFTGDEARTRATAWQTA
DWVQEVQRRGAGEIVLNMMNQDGVRSGYDLRQLEQIRAVCRVPLIASGGAGAPEHFLAAFSQADVDGALAASVFHKQIIQ
IGELKRYLALNGVEIRLC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=81.0077519379845, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI87082028, Length=212, Percent_Identity=25.4716981132075, Blast_Score=64, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=30.5732484076433, Blast_Score=132, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_EDWI9 (C5BG16)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002933974.1
- ProteinModelPortal:   C5BG16
- GeneID:   7961758
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_2570
- OMA:   ASVFHKG
- ProtClustDB:   CLSK2813362
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 28089; Mature: 28089

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRHHEIIGDIVPLARRYADEGADELVFYDITASADGRV
CCHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAAAIDIPFCVAGGIRSVKEAGLLLSYGADKISINSPALAEPTLITRLADRF
ECHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEECCCCEEECCCCCCCCHHHHHHHHHHH
GVQCVVVGIDTWHDAASDSDRVYQFTGDEARTRATAWQTADWVQEVQRRGAGEIVLNMMN
CCEEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHC
QDGVRSGYDLRQLEQIRAVCRVPLIASGGAGAPEHFLAAFSQADVDGALAASVFHKQIIQ
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IGELKRYLALNGVEIRLC
HHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRHHEIIGDIVPLARRYADEGADELVFYDITASADGRV
CCHHHCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVARVAAAIDIPFCVAGGIRSVKEAGLLLSYGADKISINSPALAEPTLITRLADRF
ECHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEECCCCEEECCCCCCCCHHHHHHHHHHH
GVQCVVVGIDTWHDAASDSDRVYQFTGDEARTRATAWQTADWVQEVQRRGAGEIVLNMMN
CCEEEEEEEECCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHC
QDGVRSGYDLRQLEQIRAVCRVPLIASGGAGAPEHFLAAFSQADVDGALAASVFHKQIIQ
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
IGELKRYLALNGVEIRLC
HHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA