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The map label for this gene is pntA [H]

Identifier: 238919856

GI number: 238919856

Start: 1887982

End: 1889508

Strand: Direct

Name: pntA [H]

Synonym: NT01EI_1960

Alternate gene names: 238919856

Gene position: 1887982-1889508 (Clockwise)

Preceding gene: 238919853

Following gene: 238919857

Centisome position: 49.52

GC content: 57.96

Gene sequence:

>1527_bases
ATGCATATTGGTGTACCAAAAGAGCGGTTGGCCAATGAAGCCCGCGTAGCGGCGACGCCGAAAACGGTAGAGCAGTTGCT
AAAGCTGGGTTTCACGGTGACTATCGAAAGCGGCGCAGGAAAACTGGCCAGCTTTGAGGATGCAGCCTATCAGCAGGCCG
GTGCCAAGGTGGTTGAACTTGATGAGGTTTGGCAGTCTGACGTGATTTTAAAGGTCAACGCGCCGGATGATGATGAAATT
GCCCGCATTAAGGCAGGTGCTACCCTGATAAGCTTTATCTGGCCGGCACAGAATCAGCCGCTGCTGGAGAAACTGGCCAA
GCGTCAGGTCACGGTGATGGCGATGGATTCGGTGCCGCGTATTTCGCGCGCCCAGTCGATGGACGCCCTCAGCTCCATGG
CAAACATTGCTGGCTACCGTGCCATCGTAGAGGCCGCCCATGAATTTGGCCGCTTCTTTACCGGTCAGATCACGGCGGCA
GGTAAGGTGCCACCGGCGAAGGTGATGGTTATCGGCGCCGGCGTTGCCGGCCTGGCCGCCATCGGTGCTGCCGGTAGCCT
CGGTGCTATCGTGCGCGCTTTCGATACCCGCCCGGAAGTAAAAGAGCAGGTGCAAAGTATGGGCGCCGAATTTCTGGAGC
TGGATTTTGAAGAAGAGGCAGGCAGCGGTGACGGCTATGCCAAAGTGATGTCTGAGGCCTTCATCAAGGCTGAGATGGCG
CTGTTTGCCGCCCAGGCACAAGAGGTGGATATTATCGTCACCACCGCGCTGATCCCAGGGAAACCGGCACCGCGCCTAAT
CACCAAAGAGATGCTCGCCACCATGAAGCCGGGCAGTGTGATCGTCGACCTGGCGGCGCAGACCGGCGGTAACTGTGAGC
TGACGCAGGCCGATCAGGTGGTGACCACGGAAAACGGCGTCAAGATCATCGGCTATACCGATCTGCCTAGCCGCCTGCCG
ACCCAGTCATCCCAGCTATATGCCACCAACCAGGTCAATCTACTCAAACTGCTGTGCAAAGAGAAAGATGGCACCCTTAA
TGTGGACTTTGAGGATGTAATCATCCGCGGGGTGACCGTGGTGCGGACAGGAGAGATCACCTGGCCGGCACCACCGATCA
AGGTTTCCGCCCAGCCACAGGCCGCCAAGACAGAACCGGTGGCAGCCCCCGTCGCCAAGCCGACGTCACCGCTGAAGAAG
TACCTGCTGATGGCTCTGGCCATTGTGCTGTTCGGCTGGATGGCCAGCGTGGCGCCAAAAGAGTTCCTGTCGCACTTTAC
CGTGTTTGCGCTCTCCTGCGTGGTGGGCTACTACGTGGTCTGGAACGTTAGCCATGCGCTGCATACGCCGCTGATGTCGG
TCACCAACGCCATCTCCGGCATCATTGTGGTCGGTGCGCTGCTGCAGATTGGGCACGGTGGTTGGATCACGTTCTTTGCC
TTTATTGCGGTGCTGATCGCCAGTATCAACATCTTCGGTGGTTTCACCGTCACCCAGCGCATGCTGAAAATGTTTCGTAA
GAACTGA

Upstream 100 bases:

>100_bases
TAGTTTATAACAAGCTATCAGCAATTTTCGCTAACAGCATACAAGGTCATCGCAGGGTATGGATGACGCTCATCTTATTG
ACAGGTGAAAAGGGACGATT

Downstream 100 bases:

>100_bases
GGGAGGTAACAAATGTCTGGAGGATTAGTCACCGCAGCATACATTGTTGCCGCCATCCTGTTTATTTTTAGTCTGGCCGG
GCTGTCCAAGCACGAGACGT

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MHIGVPKERLANEARVAATPKTVEQLLKLGFTVTIESGAGKLASFEDAAYQQAGAKVVELDEVWQSDVILKVNAPDDDEI
ARIKAGATLISFIWPAQNQPLLEKLAKRQVTVMAMDSVPRISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEMA
LFAAQAQEVDIIVTTALIPGKPAPRLITKEMLATMKPGSVIVDLAAQTGGNCELTQADQVVTTENGVKIIGYTDLPSRLP
TQSSQLYATNQVNLLKLLCKEKDGTLNVDFEDVIIRGVTVVRTGEITWPAPPIKVSAQPQAAKTEPVAAPVAKPTSPLKK
YLLMALAIVLFGWMASVAPKEFLSHFTVFALSCVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWITFFA
FIAVLIASINIFGGFTVTQRMLKMFRKN

Sequences:

>Translated_508_residues
MHIGVPKERLANEARVAATPKTVEQLLKLGFTVTIESGAGKLASFEDAAYQQAGAKVVELDEVWQSDVILKVNAPDDDEI
ARIKAGATLISFIWPAQNQPLLEKLAKRQVTVMAMDSVPRISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEMA
LFAAQAQEVDIIVTTALIPGKPAPRLITKEMLATMKPGSVIVDLAAQTGGNCELTQADQVVTTENGVKIIGYTDLPSRLP
TQSSQLYATNQVNLLKLLCKEKDGTLNVDFEDVIIRGVTVVRTGEITWPAPPIKVSAQPQAAKTEPVAAPVAKPTSPLKK
YLLMALAIVLFGWMASVAPKEFLSHFTVFALSCVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWITFFA
FIAVLIASINIFGGFTVTQRMLKMFRKN
>Mature_508_residues
MHIGVPKERLANEARVAATPKTVEQLLKLGFTVTIESGAGKLASFEDAAYQQAGAKVVELDEVWQSDVILKVNAPDDDEI
ARIKAGATLISFIWPAQNQPLLEKLAKRQVTVMAMDSVPRISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
GKVPPAKVMVIGAGVAGLAAIGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEMA
LFAAQAQEVDIIVTTALIPGKPAPRLITKEMLATMKPGSVIVDLAAQTGGNCELTQADQVVTTENGVKIIGYTDLPSRLP
TQSSQLYATNQVNLLKLLCKEKDGTLNVDFEDVIIRGVTVVRTGEITWPAPPIKVSAQPQAAKTEPVAAPVAKPTSPLKK
YLLMALAIVLFGWMASVAPKEFLSHFTVFALSCVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWITFFA
FIAVLIASINIFGGFTVTQRMLKMFRKN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=532, Percent_Identity=49.4360902255639, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI122939153, Length=532, Percent_Identity=49.4360902255639, Blast_Score=471, Evalue=1e-133,
Organism=Escherichia coli, GI1787887, Length=515, Percent_Identity=82.1359223300971, Blast_Score=850, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17550456, Length=517, Percent_Identity=52.2243713733075, Blast_Score=496, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 54269; Mature: 54269

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIGVPKERLANEARVAATPKTVEQLLKLGFTVTIESGAGKLASFEDAAYQQAGAKVVEL
CCCCCCHHHHCCCHHCCCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCEEEEH
DEVWQSDVILKVNAPDDDEIARIKAGATLISFIWPAQNQPLLEKLAKRQVTVMAMDSVPR
HHHHCCCEEEEECCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCEEEEEECCCCC
ISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEMA
HCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHEECCHHCCCCCCHHHHHHHHHHHHHHHH
LFAAQAQEVDIIVTTALIPGKPAPRLITKEMLATMKPGSVIVDLAAQTGGNCELTQADQV
HHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCE
VTTENGVKIIGYTDLPSRLPTQSSQLYATNQVNLLKLLCKEKDGTLNVDFEDVIIRGVTV
EEECCCEEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCEEECCHHHHHHHCEEE
VRTGEITWPAPPIKVSAQPQAAKTEPVAAPVAKPTSPLKKYLLMALAIVLFGWMASVAPK
EEECCEECCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
EFLSHFTVFALSCVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWITFFA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MHIGVPKERLANEARVAATPKTVEQLLKLGFTVTIESGAGKLASFEDAAYQQAGAKVVEL
CCCCCCHHHHCCCHHCCCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCEEEEH
DEVWQSDVILKVNAPDDDEIARIKAGATLISFIWPAQNQPLLEKLAKRQVTVMAMDSVPR
HHHHCCCEEEEECCCCCHHHHHHHHCCCEEEEECCCCCCHHHHHHHHCCEEEEEECCCCC
ISRAQSMDALSSMANIAGYRAIVEAAHEFGRFFTGQITAAGKVPPAKVMVIGAGVAGLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECEEEECCCCCCCEEEEEECCHHHHHH
IGAAGSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFEEEAGSGDGYAKVMSEAFIKAEMA
HCCCCCHHHHHHHHCCCHHHHHHHHHCCCHHHEECCHHCCCCCCHHHHHHHHHHHHHHHH
LFAAQAQEVDIIVTTALIPGKPAPRLITKEMLATMKPGSVIVDLAAQTGGNCELTQADQV
HHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCCCE
VTTENGVKIIGYTDLPSRLPTQSSQLYATNQVNLLKLLCKEKDGTLNVDFEDVIIRGVTV
EEECCCEEEEEECCCCCCCCCCCCCEEECCCHHHHHHHHCCCCCEEECCHHHHHHHCEEE
VRTGEITWPAPPIKVSAQPQAAKTEPVAAPVAKPTSPLKKYLLMALAIVLFGWMASVAPK
EEECCEECCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHH
EFLSHFTVFALSCVVGYYVVWNVSHALHTPLMSVTNAISGIIVVGALLQIGHGGWITFFA
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FIAVLIASINIFGGFTVTQRMLKMFRKN
HHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]