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The map label for this gene is potD [H]

Identifier: 238919712

GI number: 238919712

Start: 1750733

End: 1751572

Strand: Reverse

Name: potD [H]

Synonym: NT01EI_1815

Alternate gene names: 238919712

Gene position: 1751572-1750733 (Counterclockwise)

Preceding gene: 238919713

Following gene: 238919711

Centisome position: 45.95

GC content: 55.71

Gene sequence:

>840_bases
ATGAAAAAGTGGTCACCTCTGGTAGCCGCAGGTTTGCTGGCAATGGGAGCTCATGCCGCACATGCTGCCGATGCGCCGAA
AACCGACAATAATACGGTCTACTTCTACAACTGGACCGAATATGTCCCGCCGGGACTGCTGGAGCAGTTCACCAGGGAAA
CCGGAATCAAAGTCATCTATTCGACTTATGAATCCAACGAAACCATGTACGCCAAGCTGAAGACGTACCGGGAAGGCGCC
TACGATCTGGTGGTACCCTCCACCTACTATGTCGCCAAGATGCGTAACGAAGGGATGCTGCAAAAGATCGATCGCGATAA
GCTGAGCAACTTCCACAACCTGGATCCCAATCTGCTACACAAGTCGTTTGACCCGAAAAATGACTACTCCATCCCCTACA
TCTGGGGTGCGACGGCGATCGGGGTCAACAGCGATGTCATCGATCCAGCCAGCGTGACCAGCTGGGCCGACCTGTGGAAG
CCGGAGTATAAGGGCAGCCTGCTGCTGACGGACGATGCGCGCGAAGTGTTCCAGATAGCCCTGCGCAAGCTGGGTTACTC
AGGCAATACCACCGATCCCAAGCAGATCCAGGCGGCCTATCAGGAGCTGGTAAAGCTGATGCCCAACGTACTGGCCTTCA
ACTCCGATAACCCGGCCAACCCGTTTATGGAGGGCGAGGTCAATCTGGGCATGATCTGGAATGGCTCCGCCTATGTGGCG
CGTCAGGCCGACACCCCGGTGCAGATCGTCTGGCCAAAAAGAGGGCGCCATTTTCTGGATGGACAGCCTGGCTATTCCGG
CCAATGCCAAAAACCCCGAAGGCGCGATGAAGCTGATTAA

Upstream 100 bases:

>100_bases
GCTCCTATAATAGCCCGCCTGTTTGTTGTGGTACGCCAAGGTGTTACGACAGCGGACAGTTCCATCTTCTGTTTCGACCT
TCACTATAGGGATACGCTGA

Downstream 100 bases:

>100_bases
CTTCCTGCTGCGCCCGGACGTCGCCGCGCAGGTCGCGGAAACCATCGGCTATCCAACCCCGAACCTGGCGGCCCAAAAGC
TGCTGCCTAAAGAGGTGGTC

Product: spermidine/putrescine-binding periplasmic protein (spbp)

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKKWSPLVAAGLLAMGAHAAHAADAPKTDNNTVYFYNWTEYVPPGLLEQFTRETGIKVIYSTYESNETMYAKLKTYREGA
YDLVVPSTYYVAKMRNEGMLQKIDRDKLSNFHNLDPNLLHKSFDPKNDYSIPYIWGATAIGVNSDVIDPASVTSWADLWK
PEYKGSLLLTDDAREVFQIALRKLGYSGNTTDPKQIQAAYQELVKLMPNVLAFNSDNPANPFMEGEVNLGMIWNGSAYVA
RQADTPVQIVWPKRGRHFLDGQPGYSGQCQKPRRRDEAD

Sequences:

>Translated_279_residues
MKKWSPLVAAGLLAMGAHAAHAADAPKTDNNTVYFYNWTEYVPPGLLEQFTRETGIKVIYSTYESNETMYAKLKTYREGA
YDLVVPSTYYVAKMRNEGMLQKIDRDKLSNFHNLDPNLLHKSFDPKNDYSIPYIWGATAIGVNSDVIDPASVTSWADLWK
PEYKGSLLLTDDAREVFQIALRKLGYSGNTTDPKQIQAAYQELVKLMPNVLAFNSDNPANPFMEGEVNLGMIWNGSAYVA
RQADTPVQIVWPKRGRHFLDGQPGYSGQCQKPRRRDEAD
>Mature_279_residues
MKKWSPLVAAGLLAMGAHAAHAADAPKTDNNTVYFYNWTEYVPPGLLEQFTRETGIKVIYSTYESNETMYAKLKTYREGA
YDLVVPSTYYVAKMRNEGMLQKIDRDKLSNFHNLDPNLLHKSFDPKNDYSIPYIWGATAIGVNSDVIDPASVTSWADLWK
PEYKGSLLLTDDAREVFQIALRKLGYSGNTTDPKQIQAAYQELVKLMPNVLAFNSDNPANPFMEGEVNLGMIWNGSAYVA
RQADTPVQIVWPKRGRHFLDGQPGYSGQCQKPRRRDEAD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=258, Percent_Identity=79.0697674418605, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1787078, Length=274, Percent_Identity=33.5766423357664, Blast_Score=137, Evalue=6e-34,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 31486; Mature: 31486

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWSPLVAAGLLAMGAHAAHAADAPKTDNNTVYFYNWTEYVPPGLLEQFTRETGIKVIY
CCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEECHHHCCHHHHHHHHHHCCCEEEE
STYESNETMYAKLKTYREGAYDLVVPSTYYVAKMRNEGMLQKIDRDKLSNFHNLDPNLLH
EEECCCCEEEEEHHHHHCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHH
KSFDPKNDYSIPYIWGATAIGVNSDVIDPASVTSWADLWKPEYKGSLLLTDDAREVFQIA
CCCCCCCCCCCCEEECCEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHH
LRKLGYSGNTTDPKQIQAAYQELVKLMPNVLAFNSDNPANPFMEGEVNLGMIWNGSAYVA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEECCEEEEEEECCCEEEE
RQADTPVQIVWPKRGRHFLDGQPGYSGQCQKPRRRDEAD
ECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKWSPLVAAGLLAMGAHAAHAADAPKTDNNTVYFYNWTEYVPPGLLEQFTRETGIKVIY
CCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEEEECHHHCCHHHHHHHHHHCCCEEEE
STYESNETMYAKLKTYREGAYDLVVPSTYYVAKMRNEGMLQKIDRDKLSNFHNLDPNLLH
EEECCCCEEEEEHHHHHCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHH
KSFDPKNDYSIPYIWGATAIGVNSDVIDPASVTSWADLWKPEYKGSLLLTDDAREVFQIA
CCCCCCCCCCCCEEECCEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHH
LRKLGYSGNTTDPKQIQAAYQELVKLMPNVLAFNSDNPANPFMEGEVNLGMIWNGSAYVA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEECCEEEEEEECCCEEEE
RQADTPVQIVWPKRGRHFLDGQPGYSGQCQKPRRRDEAD
ECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]