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The map label for this gene is potD [H]

Identifier: 238919711

GI number: 238919711

Start: 1750513

End: 1750746

Strand: Reverse

Name: potD [H]

Synonym: NT01EI_1814

Alternate gene names: 238919711

Gene position: 1750746-1750513 (Counterclockwise)

Preceding gene: 238919712

Following gene: 238919702

Centisome position: 45.92

GC content: 59.4

Gene sequence:

>234_bases
ATGAAGCTGATTAACTTCCTGCTGCGCCCGGACGTCGCCGCGCAGGTCGCGGAAACCATCGGCTATCCAACCCCGAACCT
GGCGGCCCAAAAGCTGCTGCCTAAAGAGGTGGTCAGCGACAAGTCGCTCTATCCGGATGCGGAGACCATCGCCAAAGGCG
AGTGGCAGAATGACGTCGGCAGCGCCAGCGAAATCTACGAAACCGATTTCCAGAAGCTGAAGGCCGGACGCTAA

Upstream 100 bases:

>100_bases
GCCGACACCCCGGTGCAGATCGTCTGGCCAAAAAGAGGGCGCCATTTTCTGGATGGACAGCCTGGCTATTCCGGCCAATG
CCAAAAACCCCGAAGGCGCG

Downstream 100 bases:

>100_bases
TCCCCCAGCGCAGGGGGAGCCTCTCCCCCTGCCATCGCCCCTCAGGCCACGCTCTTAAACGCGCGGATCCGCTGCAGATG
CGGTGACAGGCTGGAGAACT

Product: spermidine/putrescine-binding periplasmic protein (spbp)

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 77; Mature: 77

Protein sequence:

>77_residues
MKLINFLLRPDVAAQVAETIGYPTPNLAAQKLLPKEVVSDKSLYPDAETIAKGEWQNDVGSASEIYETDFQKLKAGR

Sequences:

>Translated_77_residues
MKLINFLLRPDVAAQVAETIGYPTPNLAAQKLLPKEVVSDKSLYPDAETIAKGEWQNDVGSASEIYETDFQKLKAGR
>Mature_77_residues
MKLINFLLRPDVAAQVAETIGYPTPNLAAQKLLPKEVVSDKSLYPDAETIAKGEWQNDVGSASEIYETDFQKLKAGR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=77, Percent_Identity=80.5194805194805, Blast_Score=129, Evalue=4e-32,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 8494; Mature: 8494

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLINFLLRPDVAAQVAETIGYPTPNLAAQKLLPKEVVSDKSLYPDAETIAKGEWQNDVG
CHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
SASEIYETDFQKLKAGR
CHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLINFLLRPDVAAQVAETIGYPTPNLAAQKLLPKEVVSDKSLYPDAETIAKGEWQNDVG
CHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCC
SASEIYETDFQKLKAGR
CHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]