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The map label for this gene is pyrD

Identifier: 238919286

GI number: 238919286

Start: 1364615

End: 1365625

Strand: Direct

Name: pyrD

Synonym: NT01EI_1376

Alternate gene names: 238919286

Gene position: 1364615-1365625 (Clockwise)

Preceding gene: 238919284

Following gene: 238919287

Centisome position: 35.79

GC content: 51.34

Gene sequence:

>1011_bases
ATGTTCTACCCTTTTATCAGGAAGGCCCTCTTTCAACTCGATCCGGAACGCGCGCACGAGTTTACCTTCAGGCAACTGCA
TCGCATCAGCGGCACGCCACTCATCGGACTGCTCCGCCAGTCGGTACCGAGTAAACCGGTCAGCTGCATGGGGTTGTCAT
TTAAAAATCCGCTCGGTCTGGCTGCAGGGCTGGATAAGGATGGGGAGTGCATTGATGCGCTGGGTGCCATGGGATTCGGC
TCGATCGAAGTGGGCACGGTCACGCCGCGCCCCCAGCCGGGTAATGAAAAGCCGCGTTTATTCCGCATTGTTGAAGCGGA
GGGGCTAATTAACCGCATGGGCTTTAACAATCTGGGCGTGGATAACCTGGTTGAGAATGTCAAAAAATCTCACTTCGGTG
GCATTCTTGGGATTAATATTGGCAAAAATAAAGATACGCCGGTAGAGCAGGGTAAAGACGACTATCTGATGTGCATGGAT
AAGGTGTATTCCTATGCTGGCTATATTGCCGTAAATATCTCTTCGCCTAACACGCCGGGGTTACGTACGCTGCAATATGG
CGAGGCATTGGATGATTTACTGGCTGCGATTAAAACCAAGCAGGCCGAGCTGCAGCAAAAACATGCCAGATATGTCCCGG
TCGCTGTAAAAATTGCGCCGGATTTGAGCCATGAAGAGCTGGTGCAGATTGCCGATAGCCTGGTGCGTCATCAAATTGAT
GGGGTGATTGCCACTAATACAACGCTGGATCGTTCGTTGGTCAATGGGCTTAATCATTGTGCCCAGAGCGGTGGCCTGAG
TGGGCGCCCGCTGCAGTTGAAAAGCACCGAGATTATTCGCCAGCTTTCAGCTGAGCTGCAGGGGCGACTGCCTATTATTG
GCGTCGGTGGTATTGATTCAGTGATTGCCGCCCGTGAAAAAATGCAGGCGGGAGCATCCTTAGTGCAGATTTATTCCGGC
TTTATCTTTAAAGGGCCGGGTTTGGTTAAAGAAATCGTTAATCACATCTAA

Upstream 100 bases:

>100_bases
GTTTGCTTTTATAGGGCTCTTTTTGTCTTTAATCAATTCCAATTGAGGTCAACTTACCTGATAATAACGCCCCGTTTGGC
AACGGGAATCAGGAGATCTC

Downstream 100 bases:

>100_bases
TTTTTAATCCTGCCTAATTGAGCCCGGGACTTTATTTTAGTCACGGGCTAGTCTATATTTTCACCAGTATGTCGCTTATT
TGCTCAGATGCTGAGTTAAT

Product: dihydroorotate oxidase

Products: NA

Alternate protein names: DHOdehase; DHOD; DHODase; Dihydroorotate oxidase

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MFYPFIRKALFQLDPERAHEFTFRQLHRISGTPLIGLLRQSVPSKPVSCMGLSFKNPLGLAAGLDKDGECIDALGAMGFG
SIEVGTVTPRPQPGNEKPRLFRIVEAEGLINRMGFNNLGVDNLVENVKKSHFGGILGINIGKNKDTPVEQGKDDYLMCMD
KVYSYAGYIAVNISSPNTPGLRTLQYGEALDDLLAAIKTKQAELQQKHARYVPVAVKIAPDLSHEELVQIADSLVRHQID
GVIATNTTLDRSLVNGLNHCAQSGGLSGRPLQLKSTEIIRQLSAELQGRLPIIGVGGIDSVIAAREKMQAGASLVQIYSG
FIFKGPGLVKEIVNHI

Sequences:

>Translated_336_residues
MFYPFIRKALFQLDPERAHEFTFRQLHRISGTPLIGLLRQSVPSKPVSCMGLSFKNPLGLAAGLDKDGECIDALGAMGFG
SIEVGTVTPRPQPGNEKPRLFRIVEAEGLINRMGFNNLGVDNLVENVKKSHFGGILGINIGKNKDTPVEQGKDDYLMCMD
KVYSYAGYIAVNISSPNTPGLRTLQYGEALDDLLAAIKTKQAELQQKHARYVPVAVKIAPDLSHEELVQIADSLVRHQID
GVIATNTTLDRSLVNGLNHCAQSGGLSGRPLQLKSTEIIRQLSAELQGRLPIIGVGGIDSVIAAREKMQAGASLVQIYSG
FIFKGPGLVKEIVNHI
>Mature_336_residues
MFYPFIRKALFQLDPERAHEFTFRQLHRISGTPLIGLLRQSVPSKPVSCMGLSFKNPLGLAAGLDKDGECIDALGAMGFG
SIEVGTVTPRPQPGNEKPRLFRIVEAEGLINRMGFNNLGVDNLVENVKKSHFGGILGINIGKNKDTPVEQGKDDYLMCMD
KVYSYAGYIAVNISSPNTPGLRTLQYGEALDDLLAAIKTKQAELQQKHARYVPVAVKIAPDLSHEELVQIADSLVRHQID
GVIATNTTLDRSLVNGLNHCAQSGGLSGRPLQLKSTEIIRQLSAELQGRLPIIGVGGIDSVIAAREKMQAGASLVQIYSG
FIFKGPGLVKEIVNHI

Specific function: Pyrimidine biosynthesis; fourth step. [C]

COG id: COG0167

COG function: function code F; Dihydroorotate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily

Homologues:

Organism=Homo sapiens, GI45006951, Length=332, Percent_Identity=43.0722891566265, Blast_Score=226, Evalue=2e-59,
Organism=Escherichia coli, GI1787177, Length=336, Percent_Identity=84.2261904761905, Blast_Score=599, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI17509475, Length=335, Percent_Identity=41.1940298507463, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI281361352, Length=294, Percent_Identity=41.8367346938776, Blast_Score=231, Evalue=7e-61,
Organism=Drosophila melanogaster, GI17137316, Length=294, Percent_Identity=41.8367346938776, Blast_Score=231, Evalue=7e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRD_EDWI9 (C5BD71)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002932801.1
- GeneID:   7960459
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_1376
- OMA:   SYVTVNI
- ProtClustDB:   CLSK2813045
- HAMAP:   MF_00225
- InterPro:   IPR013785
- InterPro:   IPR012135
- InterPro:   IPR005719
- InterPro:   IPR001295
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF000164
- TIGRFAMs:   TIGR01036

Pfam domain/function: PF01180 DHO_dh

EC number: =1.3.5.2

Molecular weight: Translated: 36463; Mature: 36463

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00911 DHODEHASE_1; PS00912 DHODEHASE_2

Important sites: ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYPFIRKALFQLDPERAHEFTFRQLHRISGTPLIGLLRQSVPSKPVSCMGLSFKNPLGL
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEECCCCCCCCCH
AAGLDKDGECIDALGAMGFGSIEVGTVTPRPQPGNEKPRLFRIVEAEGLINRMGFNNLGV
HCCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCH
DNLVENVKKSHFGGILGINIGKNKDTPVEQGKDDYLMCMDKVYSYAGYIAVNISSPNTPG
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCC
LRTLQYGEALDDLLAAIKTKQAELQQKHARYVPVAVKIAPDLSHEELVQIADSLVRHQID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHC
GVIATNTTLDRSLVNGLNHCAQSGGLSGRPLQLKSTEIIRQLSAELQGRLPIIGVGGIDS
CEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEECCCHHH
VIAAREKMQAGASLVQIYSGFIFKGPGLVKEIVNHI
HHHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHCC
>Mature Secondary Structure
MFYPFIRKALFQLDPERAHEFTFRQLHRISGTPLIGLLRQSVPSKPVSCMGLSFKNPLGL
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEECCCCCCCCCH
AAGLDKDGECIDALGAMGFGSIEVGTVTPRPQPGNEKPRLFRIVEAEGLINRMGFNNLGV
HCCCCCCCHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCEEEEEEHHHHHHHCCCCCCCH
DNLVENVKKSHFGGILGINIGKNKDTPVEQGKDDYLMCMDKVYSYAGYIAVNISSPNTPG
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCC
LRTLQYGEALDDLLAAIKTKQAELQQKHARYVPVAVKIAPDLSHEELVQIADSLVRHQID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHC
GVIATNTTLDRSLVNGLNHCAQSGGLSGRPLQLKSTEIIRQLSAELQGRLPIIGVGGIDS
CEEEECCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEECCCHHH
VIAAREKMQAGASLVQIYSGFIFKGPGLVKEIVNHI
HHHHHHHHHHHHHHHHHHHCHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA