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The map label for this gene is tilS [H]

Identifier: 238917789

GI number: 238917789

Start: 1933863

End: 1935323

Strand: Reverse

Name: tilS [H]

Synonym: EUBELI_01870

Alternate gene names: 238917789

Gene position: 1935323-1933863 (Counterclockwise)

Preceding gene: 238917790

Following gene: 238917788

Centisome position: 90.26

GC content: 40.86

Gene sequence:

>1461_bases
ATGAATGAAATTATAGATAAGATTTACAGATATATTGAACAGAATAATATGCTTACGGACTGCGAAAGAGTGGTTGTCGG
TCTTTCAGGCGGCGCGGATTCTGTATGTCTGCTTATGGTGCTTAAGGGGTATATTGAGAGGAGGCGTCTACAGACAGAGT
TATGTGCGGTGCATGTCAATCATGGCATAAGGCAGGAGGCAGGAGATGATGAGGAGTTTGCCAGAGCCTTGTGTGAACGC
ATGGGTGTTGAATTCATGGCATATCATATAGATGCCGCCGGGCTTGCAAAGCAGCTTGGAATGAGTGTTGAGGAAGCAGG
AAGGAAAGAACGGTACCGTATATTCAATGAGGCATGTAAAGGGCGTAATGCAAGGATTGCGGTTGCACACCATATGAATG
ACCAGGCAGAAACGGTTCTTATGAATCTTTCCCGAGGAACTTCACTTAAAGGAATAGGTGGAATCAGACCGGTAAGGGAT
AATATTATAAGACCTCTGCTTTCAGTGACAAGAGCAGAGGTTGAAGAAGTACTTAAAGACTTTAACCAGCCATATGTTAC
TGATGCAACGAATCTGTGCAATGATTATACGCGTAATTCTTTAAGAAATGTTGTTATTCCGTACATGACAGAAAAGGTTA
ATGCACACACTGTTGAAAATATTGCTGATGCAGCAGAGGAGCTGCAGAAGAATTTTGATTTTATTGAGGCAGAGGCCAAA
AAGGCTTATGATAAGCATGTATATGTGGGAGATACGGTGGTTTTAAGGCTTTATGGGGAGGAGTTTGCAGGACTTCATGA
GGTCATAAGAAAGAGAGTTATCTACAAGGCAGTACATGCACTTACACAGACAGCAAAGGATATATATAAGGTTCATGTGA
ATGCTGTAGATGAGCTTATACGAAAACAGGTTGGCAGCAGTGCAGATATATGTTATGGACTGTGTGCGGTAAAAGGGTAT
GAGGATATCACAATAAGAAGGAAAAATGTGGAATCTCGGACACAGGTTTCTTCGGACCTCATACATGTACTTACGCCGCA
GGAGCTTAAAAGACTTAATTCAGGTGAAAATATTACCATAGAAGAAAACATTTATTATAATAATGATGGCAAGACGGAAT
TAAGAAAGGTGCATATTGTAATTTCACTTCATTCTAATTATGAAAAAAAAAGAAATTACGCTAATAGTTGCTATGCGAAA
TCCTTTGATTATGATAAAATACAAGGAAAGCTCTGCATAAGACACCGTACTGACGGCGACAGGATTGTTGTGAACAGGGC
GGGAACATGGCGCAAGCTGAAAAAAGAATTCATTGACCGTAAAGTACCTGCCGATATGAGAGATAATGTGCTTGTTGTTT
CTGATGATTCGGGAGTTTTATGGGCTGTCGGGGTAAGAAGAAGTGAGTGCGCCCTGATCGATGATGAAACAAGGAATGTG
CTGGATATAACTATACAATAG

Upstream 100 bases:

>100_bases
GGAGTCGCTCAAGAATCATAATATGGAGGCTTTTGATGACTGTACAGTTATGGTACTGGGAGTGTTTGACACTTATGTGA
ACGTATAATATAATTCTTGC

Downstream 100 bases:

>100_bases
AGTGAATATTAAGATTGACAAAGGAGAGAAACGATGAAAGAACCATTTATCAGTGTCCTTATATCTGAGGAAAAGCTGAA
TGAAAGGATTGCACAGCTTG

Product: cell cycle protein MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MNEIIDKIYRYIEQNNMLTDCERVVVGLSGGADSVCLLMVLKGYIERRRLQTELCAVHVNHGIRQEAGDDEEFARALCER
MGVEFMAYHIDAAGLAKQLGMSVEEAGRKERYRIFNEACKGRNARIAVAHHMNDQAETVLMNLSRGTSLKGIGGIRPVRD
NIIRPLLSVTRAEVEEVLKDFNQPYVTDATNLCNDYTRNSLRNVVIPYMTEKVNAHTVENIADAAEELQKNFDFIEAEAK
KAYDKHVYVGDTVVLRLYGEEFAGLHEVIRKRVIYKAVHALTQTAKDIYKVHVNAVDELIRKQVGSSADICYGLCAVKGY
EDITIRRKNVESRTQVSSDLIHVLTPQELKRLNSGENITIEENIYYNNDGKTELRKVHIVISLHSNYEKKRNYANSCYAK
SFDYDKIQGKLCIRHRTDGDRIVVNRAGTWRKLKKEFIDRKVPADMRDNVLVVSDDSGVLWAVGVRRSECALIDDETRNV
LDITIQ

Sequences:

>Translated_486_residues
MNEIIDKIYRYIEQNNMLTDCERVVVGLSGGADSVCLLMVLKGYIERRRLQTELCAVHVNHGIRQEAGDDEEFARALCER
MGVEFMAYHIDAAGLAKQLGMSVEEAGRKERYRIFNEACKGRNARIAVAHHMNDQAETVLMNLSRGTSLKGIGGIRPVRD
NIIRPLLSVTRAEVEEVLKDFNQPYVTDATNLCNDYTRNSLRNVVIPYMTEKVNAHTVENIADAAEELQKNFDFIEAEAK
KAYDKHVYVGDTVVLRLYGEEFAGLHEVIRKRVIYKAVHALTQTAKDIYKVHVNAVDELIRKQVGSSADICYGLCAVKGY
EDITIRRKNVESRTQVSSDLIHVLTPQELKRLNSGENITIEENIYYNNDGKTELRKVHIVISLHSNYEKKRNYANSCYAK
SFDYDKIQGKLCIRHRTDGDRIVVNRAGTWRKLKKEFIDRKVPADMRDNVLVVSDDSGVLWAVGVRRSECALIDDETRNV
LDITIQ
>Mature_486_residues
MNEIIDKIYRYIEQNNMLTDCERVVVGLSGGADSVCLLMVLKGYIERRRLQTELCAVHVNHGIRQEAGDDEEFARALCER
MGVEFMAYHIDAAGLAKQLGMSVEEAGRKERYRIFNEACKGRNARIAVAHHMNDQAETVLMNLSRGTSLKGIGGIRPVRD
NIIRPLLSVTRAEVEEVLKDFNQPYVTDATNLCNDYTRNSLRNVVIPYMTEKVNAHTVENIADAAEELQKNFDFIEAEAK
KAYDKHVYVGDTVVLRLYGEEFAGLHEVIRKRVIYKAVHALTQTAKDIYKVHVNAVDELIRKQVGSSADICYGLCAVKGY
EDITIRRKNVESRTQVSSDLIHVLTPQELKRLNSGENITIEENIYYNNDGKTELRKVHIVISLHSNYEKKRNYANSCYAK
SFDYDKIQGKLCIRHRTDGDRIVVNRAGTWRKLKKEFIDRKVPADMRDNVLVVSDDSGVLWAVGVRRSECALIDDETRNV
LDITIQ

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=215, Percent_Identity=28.3720930232558, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 55348; Mature: 55348

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIIDKIYRYIEQNNMLTDCERVVVGLSGGADSVCLLMVLKGYIERRRLQTELCAVHVN
CHHHHHHHHHHHHHCCCHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HGIRQEAGDDEEFARALCERMGVEFMAYHIDAAGLAKQLGMSVEEAGRKERYRIFNEACK
CCCCCCCCCHHHHHHHHHHHHCCHHEEEECCHHHHHHHHCCCHHHHCHHHHHHHHHHHHC
GRNARIAVAHHMNDQAETVLMNLSRGTSLKGIGGIRPVRDNIIRPLLSVTRAEVEEVLKD
CCCCEEEEEECCCCHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FNQPYVTDATNLCNDYTRNSLRNVVIPYMTEKVNAHTVENIADAAEELQKNFDFIEAEAK
CCCCCCCCHHHHHHHHHHHHHHCEECHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
KAYDKHVYVGDTVVLRLYGEEFAGLHEVIRKRVIYKAVHALTQTAKDIYKVHVNAVDELI
HHHCCEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKQVGSSADICYGLCAVKGYEDITIRRKNVESRTQVSSDLIHVLTPQELKRLNSGENITI
HHHCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHCCCCCCEEE
EENIYYNNDGKTELRKVHIVISLHSNYEKKRNYANSCYAKSFDYDKIQGKLCIRHRTDGD
ECCEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCC
RIVVNRAGTWRKLKKEFIDRKVPADMRDNVLVVSDDSGVLWAVGVRRSECALIDDETRNV
EEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCCCCEEECCCCCCE
LDITIQ
EEEEEC
>Mature Secondary Structure
MNEIIDKIYRYIEQNNMLTDCERVVVGLSGGADSVCLLMVLKGYIERRRLQTELCAVHVN
CHHHHHHHHHHHHHCCCHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HGIRQEAGDDEEFARALCERMGVEFMAYHIDAAGLAKQLGMSVEEAGRKERYRIFNEACK
CCCCCCCCCHHHHHHHHHHHHCCHHEEEECCHHHHHHHHCCCHHHHCHHHHHHHHHHHHC
GRNARIAVAHHMNDQAETVLMNLSRGTSLKGIGGIRPVRDNIIRPLLSVTRAEVEEVLKD
CCCCEEEEEECCCCHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FNQPYVTDATNLCNDYTRNSLRNVVIPYMTEKVNAHTVENIADAAEELQKNFDFIEAEAK
CCCCCCCCHHHHHHHHHHHHHHCEECHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
KAYDKHVYVGDTVVLRLYGEEFAGLHEVIRKRVIYKAVHALTQTAKDIYKVHVNAVDELI
HHHCCEEEECCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKQVGSSADICYGLCAVKGYEDITIRRKNVESRTQVSSDLIHVLTPQELKRLNSGENITI
HHHCCCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHCCCCCCEEE
EENIYYNNDGKTELRKVHIVISLHSNYEKKRNYANSCYAKSFDYDKIQGKLCIRHRTDGD
ECCEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCC
RIVVNRAGTWRKLKKEFIDRKVPADMRDNVLVVSDDSGVLWAVGVRRSECALIDDETRNV
EEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEECCCCCCCEEECCCCCCE
LDITIQ
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]