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The map label for this gene is spoIIE [H]

Identifier: 238917790

GI number: 238917790

Start: 1935336

End: 1936448

Strand: Reverse

Name: spoIIE [H]

Synonym: EUBELI_01871

Alternate gene names: 238917790

Gene position: 1936448-1935336 (Counterclockwise)

Preceding gene: 238917791

Following gene: 238917789

Centisome position: 90.31

GC content: 39.26

Gene sequence:

>1113_bases
ATGAAAAATACATATAAAGATGCCGGATATACTTATACTATTAACCGTCTGCAGGAAACAGCCAGAACATTCAGAAATCT
TGGAGATGCATATGGAGAAACTAATCAGAAGCAGACGGGGTTTAAAAGACAATTGATTCTGGCTGCAGATATATTAGAGG
AATGTGTTGCAATGAATCTTGATGCTAAAGCGCCGGATAAACAGGAGCGCAGGGAATTTGAGCGTAAATGTATGGCAATG
GGGATATCTGTTAAGGATGTAAAACTGGTTGACGGAAAGAGGCGCGAGATACTTGTAACTGCGAAAACATTTATGAAGGG
GTGTGTGTCTGAGCGTGTATTAAGAGAAACGGTGAGCAGTGTATTTAAGGCAAAATTCTTTTCTAATCAGGACAACAGGG
TGATTATAAATGAAGAACCGGACCAGTATGTGTTCTATCAGGAGAATCGTTTCAGGATTCTTTCAGGTATGGCGAGAAAA
TGTAAAGAGGAAGAGAATACAAGTGGGGATAACTTCCTGCTAAAGAAGCTGAACTGTGGGAAAATGGTTGCAGCAATTGC
AGATGGCTGTGGAAGTGGTAAAAGGGCATTTGCAGAGAGCCGCATGGTGATTGAGCTTATGGAAAACTGCATTGAAGCGG
GATTCGAGGAAAAGACAGCGATTGATCTTATTAATTCTGCGTATATTGCGGGAACTACATTTAACAATCCGGTAACTATG
GATATGAGTATTATTGACTGTCAGGCGGGAGTTATTAACTGTATTAAACTTGGAGCGGTATCCACATTTATAAAAAGAGA
AAACTGGGTTGAGATTATTAAGTCAACAACGCTTCCGATGGGAGTGTTAGAGCAGGTGGACTACGATTGTACGACTAAAA
AGCTTTATGATGGTAATTATATTATAATGATAAGTGATGGTGTGCTTGATAATCTGTCTGGAATTAATAAGGAGGAACAG
ATGGTGGAGATTATTAATAATATAAATGTAAAGAAGCCGGCAGGTATTGCAAAAAAGATTCTTGAGGAGTCGCTCAAGAA
TCATAATATGGAGGCTTTTGATGACTGTACAGTTATGGTACTGGGAGTGTTTGACACTTATGTGAACGTATAA

Upstream 100 bases:

>100_bases
GCTGGAAGATTTCTTTGAATCTTTCATGCTTCGACATTTTATTTATAAGCAGTCATATATACTTCACATTGGTGTAGTGC
CGGAAAGGAAGTGGATATGA

Downstream 100 bases:

>100_bases
TATAATTCTTGCATGAATGAAATTATAGATAAGATTTACAGATATATTGAACAGAATAATATGCTTACGGACTGCGAAAG
AGTGGTTGTCGGTCTTTCAG

Product: protein phosphatase

Products: NA

Alternate protein names: Stage II sporulation protein H [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKNTYKDAGYTYTINRLQETARTFRNLGDAYGETNQKQTGFKRQLILAADILEECVAMNLDAKAPDKQERREFERKCMAM
GISVKDVKLVDGKRREILVTAKTFMKGCVSERVLRETVSSVFKAKFFSNQDNRVIINEEPDQYVFYQENRFRILSGMARK
CKEEENTSGDNFLLKKLNCGKMVAAIADGCGSGKRAFAESRMVIELMENCIEAGFEEKTAIDLINSAYIAGTTFNNPVTM
DMSIIDCQAGVINCIKLGAVSTFIKRENWVEIIKSTTLPMGVLEQVDYDCTTKKLYDGNYIIMISDGVLDNLSGINKEEQ
MVEIINNINVKKPAGIAKKILEESLKNHNMEAFDDCTVMVLGVFDTYVNV

Sequences:

>Translated_370_residues
MKNTYKDAGYTYTINRLQETARTFRNLGDAYGETNQKQTGFKRQLILAADILEECVAMNLDAKAPDKQERREFERKCMAM
GISVKDVKLVDGKRREILVTAKTFMKGCVSERVLRETVSSVFKAKFFSNQDNRVIINEEPDQYVFYQENRFRILSGMARK
CKEEENTSGDNFLLKKLNCGKMVAAIADGCGSGKRAFAESRMVIELMENCIEAGFEEKTAIDLINSAYIAGTTFNNPVTM
DMSIIDCQAGVINCIKLGAVSTFIKRENWVEIIKSTTLPMGVLEQVDYDCTTKKLYDGNYIIMISDGVLDNLSGINKEEQ
MVEIINNINVKKPAGIAKKILEESLKNHNMEAFDDCTVMVLGVFDTYVNV
>Mature_370_residues
MKNTYKDAGYTYTINRLQETARTFRNLGDAYGETNQKQTGFKRQLILAADILEECVAMNLDAKAPDKQERREFERKCMAM
GISVKDVKLVDGKRREILVTAKTFMKGCVSERVLRETVSSVFKAKFFSNQDNRVIINEEPDQYVFYQENRFRILSGMARK
CKEEENTSGDNFLLKKLNCGKMVAAIADGCGSGKRAFAESRMVIELMENCIEAGFEEKTAIDLINSAYIAGTTFNNPVTM
DMSIIDCQAGVINCIKLGAVSTFIKRENWVEIIKSTTLPMGVLEQVDYDCTTKKLYDGNYIIMISDGVLDNLSGINKEEQ
MVEIINNINVKKPAGIAKKILEESLKNHNMEAFDDCTVMVLGVFDTYVNV

Specific function: Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates spoIIAA by dephosphorylation [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein. Note=Polar septum [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001932
- InterPro:   IPR014221
- InterPro:   IPR010822 [H]

Pfam domain/function: PF07228 SpoIIE [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 41714; Mature: 41714

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTYKDAGYTYTINRLQETARTFRNLGDAYGETNQKQTGFKRQLILAADILEECVAMNL
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHCCC
DAKAPDKQERREFERKCMAMGISVKDVKLVDGKRREILVTAKTFMKGCVSERVLRETVSS
CCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
VFKAKFFSNQDNRVIINEEPDQYVFYQENRFRILSGMARKCKEEENTSGDNFLLKKLNCG
HHHHHHHCCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCH
KMVAAIADGCGSGKRAFAESRMVIELMENCIEAGFEEKTAIDLINSAYIAGTTFNNPVTM
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCCCCCEEE
DMSIIDCQAGVINCIKLGAVSTFIKRENWVEIIKSTTLPMGVLEQVDYDCTTKKLYDGNY
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEECCCE
IIMISDGVLDNLSGINKEEQMVEIINNINVKKPAGIAKKILEESLKNHNMEAFDDCTVMV
EEEECCCCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
LGVFDTYVNV
HHHHHHHHCC
>Mature Secondary Structure
MKNTYKDAGYTYTINRLQETARTFRNLGDAYGETNQKQTGFKRQLILAADILEECVAMNL
CCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHHHCCC
DAKAPDKQERREFERKCMAMGISVKDVKLVDGKRREILVTAKTFMKGCVSERVLRETVSS
CCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHH
VFKAKFFSNQDNRVIINEEPDQYVFYQENRFRILSGMARKCKEEENTSGDNFLLKKLNCG
HHHHHHHCCCCCEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCH
KMVAAIADGCGSGKRAFAESRMVIELMENCIEAGFEEKTAIDLINSAYIAGTTFNNPVTM
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCCCCCEEE
DMSIIDCQAGVINCIKLGAVSTFIKRENWVEIIKSTTLPMGVLEQVDYDCTTKKLYDGNY
EEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEECCCE
IIMISDGVLDNLSGINKEEQMVEIINNINVKKPAGIAKKILEESLKNHNMEAFDDCTVMV
EEEECCCCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHH
LGVFDTYVNV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7584024; 8830262; 9384377; 2832371; 8113187; 7570023 [H]