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The map label for this gene is ywkE [H]

Identifier: 238917366

GI number: 238917366

Start: 1497046

End: 1497885

Strand: Reverse

Name: ywkE [H]

Synonym: EUBELI_01443

Alternate gene names: 238917366

Gene position: 1497885-1497046 (Counterclockwise)

Preceding gene: 238917367

Following gene: 238917365

Centisome position: 69.86

GC content: 36.19

Gene sequence:

>840_bases
ATGATGACAATTAGACAGGCCGTAAACTGGGGAACACAGATGCTGAAAGATAAAGGAATTGACAATGCAGAGCATGACAG
TTTTGAATTGTTATCCGCTATTAATGGCATGACAAGAACCTTTTATCTTATCAATGGTGACAGTTTATTGTCGGAAGAAA
ATTTTCTCATGTTTGAGGAATATATAGAACAGAGAGCTTCTCATATACCACTTCAGCATATCCTTGGAAAAACATGGTTT
TATGGTTATGAATATATGGTTAACAGTGATGTCCTTATTCCAAGACAGGATACAGAGATTTTAGTTGGTGAAGTGATTAA
GGTGACAAGAAGTGGAGATTATATTCTTGATATGTGTACCGGCTCAGGATGCATAGGAATTACACTTGCCAAGAAGTTTC
CTGAATGCAGAGTGCTTGGAGTTGATGTCTCAGAAAAGGCACTAAATGTTGCACAGAGTAATAAACATAATCTTGAGGCT
GAAAATATTGATTTTATGAGAAGTGATTTATTCGAAGAATTAAATCATGATATAACATTTAATACAATAGTTTCTAATCC
TCCATATATACCAACTAAGGTTATTGAAGGACTTCAGGAGGAAGTCAGATTACATGATCCTATACTTGCACTTGATGGAA
TGGAAGATGGACTTATGTTTTATAGAAAAATAACTGCACAGGCGGGAGACTTCCTTGAAACTGATGGATATCTCTGTTAT
GAAATAGGAGCAGAGCAGGCGGCAGATGTTTCTGATATAATGAAAAAGGCTGGCTTTAAGGATATAACTGTCGTGAAAGA
TCTTGCTGGTTTTGACCGTGTTGTTATGGGAAGAAAGTAA

Upstream 100 bases:

>100_bases
TTGTTGCAGGATTTGAGTTTGAAGCTGGACAGGAACCGGAAGAGCAATATCCGGAAGATGTTCCTATGTTTAAACAGAAA
AGGCTGGAAGATAAGTAGAT

Downstream 100 bases:

>100_bases
TTGTATATAATTGATGTAGAAAGGAAAAGTGACAGAGTATGTTTGATAGACTTGAAGATACAGTCAACAGATATGAAGAT
ATAACTGCTGAACTTGGTAA

Product: HemK protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MMTIRQAVNWGTQMLKDKGIDNAEHDSFELLSAINGMTRTFYLINGDSLLSEENFLMFEEYIEQRASHIPLQHILGKTWF
YGYEYMVNSDVLIPRQDTEILVGEVIKVTRSGDYILDMCTGSGCIGITLAKKFPECRVLGVDVSEKALNVAQSNKHNLEA
ENIDFMRSDLFEELNHDITFNTIVSNPPYIPTKVIEGLQEEVRLHDPILALDGMEDGLMFYRKITAQAGDFLETDGYLCY
EIGAEQAADVSDIMKKAGFKDITVVKDLAGFDRVVMGRK

Sequences:

>Translated_279_residues
MMTIRQAVNWGTQMLKDKGIDNAEHDSFELLSAINGMTRTFYLINGDSLLSEENFLMFEEYIEQRASHIPLQHILGKTWF
YGYEYMVNSDVLIPRQDTEILVGEVIKVTRSGDYILDMCTGSGCIGITLAKKFPECRVLGVDVSEKALNVAQSNKHNLEA
ENIDFMRSDLFEELNHDITFNTIVSNPPYIPTKVIEGLQEEVRLHDPILALDGMEDGLMFYRKITAQAGDFLETDGYLCY
EIGAEQAADVSDIMKKAGFKDITVVKDLAGFDRVVMGRK
>Mature_279_residues
MMTIRQAVNWGTQMLKDKGIDNAEHDSFELLSAINGMTRTFYLINGDSLLSEENFLMFEEYIEQRASHIPLQHILGKTWF
YGYEYMVNSDVLIPRQDTEILVGEVIKVTRSGDYILDMCTGSGCIGITLAKKFPECRVLGVDVSEKALNVAQSNKHNLEA
ENIDFMRSDLFEELNHDITFNTIVSNPPYIPTKVIEGLQEEVRLHDPILALDGMEDGLMFYRKITAQAGDFLETDGYLCY
EIGAEQAADVSDIMKKAGFKDITVVKDLAGFDRVVMGRK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=288, Percent_Identity=26.3888888888889, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1787463, Length=262, Percent_Identity=31.6793893129771, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87082085, Length=203, Percent_Identity=38.9162561576355, Blast_Score=135, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324266, Length=162, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582226, Length=205, Percent_Identity=29.2682926829268, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 31517; Mature: 31517

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTIRQAVNWGTQMLKDKGIDNAEHDSFELLSAINGMTRTFYLINGDSLLSEENFLMFEE
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEECCCHHCCCCCCHHHHH
YIEQRASHIPLQHILGKTWFYGYEYMVNSDVLIPRQDTEILVGEVIKVTRSGDYILDMCT
HHHHHHHCCCHHHHHHHHHHEEEEEEECCCEEECCCCCHHHHHHHHHHCCCCCEEEEEEC
GSGCIGITLAKKFPECRVLGVDVSEKALNVAQSNKHNLEAENIDFMRSDLFEELNHDITF
CCCEEEEEECCCCCCCEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEE
NTIVSNPPYIPTKVIEGLQEEVRLHDPILALDGMEDGLMFYRKITAQAGDFLETDGYLCY
EEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEE
EIGAEQAADVSDIMKKAGFKDITVVKDLAGFDRVVMGRK
ECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMTIRQAVNWGTQMLKDKGIDNAEHDSFELLSAINGMTRTFYLINGDSLLSEENFLMFEE
CCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEECCCHHCCCCCCHHHHH
YIEQRASHIPLQHILGKTWFYGYEYMVNSDVLIPRQDTEILVGEVIKVTRSGDYILDMCT
HHHHHHHCCCHHHHHHHHHHEEEEEEECCCEEECCCCCHHHHHHHHHHCCCCCEEEEEEC
GSGCIGITLAKKFPECRVLGVDVSEKALNVAQSNKHNLEAENIDFMRSDLFEELNHDITF
CCCEEEEEECCCCCCCEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEE
NTIVSNPPYIPTKVIEGLQEEVRLHDPILALDGMEDGLMFYRKITAQAGDFLETDGYLCY
EEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEEE
EIGAEQAADVSDIMKKAGFKDITVVKDLAGFDRVVMGRK
ECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]