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The map label for this gene is prfA

Identifier: 238917365

GI number: 238917365

Start: 1495934

End: 1497007

Strand: Reverse

Name: prfA

Synonym: EUBELI_01442

Alternate gene names: 238917365

Gene position: 1497007-1495934 (Counterclockwise)

Preceding gene: 238917366

Following gene: 238917364

Centisome position: 69.82

GC content: 40.22

Gene sequence:

>1074_bases
ATGTTTGATAGACTTGAAGATACAGTCAACAGATATGAAGATATAACTGCTGAACTTGGTAATCCTGATGTGGTTAATGA
TCAGGAAAAGTTCAAAAAGCTTATGAAAGAACAGAGTAGTCTTGCACCACTTATAGAAACTTATACTGAGTATAAGAACT
GTAAGAAGAATATTGAGGACAGTCTTGCTATTCTTGATGAAGAGACAGATGAAGAACTTAAAGAGATGGCGAAGGAAGAG
CTTAATGAATCTAAGGCAAGAGTAGAAGAATTAGAGCATGAATTAAAGATTCTTCTTTTACCTAAAGACCCTAATGATGA
TAAGGATATTGTTGTTGAAATCAGAGCGGGTGCAGGTGGAGAAGAGGCGGCACTTTTTGCAGCAGAGCTTTTCAGAATGT
ATGTACACTATGCACAGGCAAGAGGCTGGAAGGTTGAAGTATTAAGTGGCGATGAAACCGGTATTGGTGGAATGAAGGAA
GTAGAATTCATGGTTAAGGGACAGGGAGCTTACTCTATCATGAAATATGAGTCTGGTGTACACAGAGTACAGAGAATCCC
GGTAACTGAATCTAACGGACGAATACAGACTTCTACTGCTTCTGTAGCTGTTATGCCAGAGGCAGAAGAGGTTGATATCC
ACATTGATGAGAAGGACATCCGTATTGATGTTATGCGAGCGTCAGGTAATGGTGGACAGTGTGTCAATACAACTGATTCT
GCTGTACGACTTACTCATTATCCAACAGGAATAGTTATTTATTCACAGACAGAGAAGTCACAGATACAGAATAAGGAGAA
AGCATTTGCACTTTTAAGAGCCAAGTTATATGACATTGAGACTCAGAAAGCACATGACGCAGAGGCAGATGCAAGAAGAA
GCCAGATTGGTACAGGTGACAGAGCTGAGAAGATAAGAACTTATAATTTCCCACAGGGAAGAGTAACAGACCACAGAATT
GGTCTTACATTATATAAGCTTGACAAGATTATGGATGGAGACATCCAGGATATTATTGATGCATGTATCGCTGCCGACCA
GGCTGCCAAGCTTGCTAAGATGGGCGAGGATTAA

Upstream 100 bases:

>100_bases
AGGATATAACTGTCGTGAAAGATCTTGCTGGTTTTGACCGTGTTGTTATGGGAAGAAAGTAATTGTATATAATTGATGTA
GAAAGGAAAAGTGACAGAGT

Downstream 100 bases:

>100_bases
TTGGTATTATGAAAAAGGATAGGAATTCTAATCCGGTTGCGCTGGTACCTATACTTGTTTTTCTTGTGTTGTATCTTGGA
ACTGGAATTATATTTGAATA

Product: peptide chain release factor RF-1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MFDRLEDTVNRYEDITAELGNPDVVNDQEKFKKLMKEQSSLAPLIETYTEYKNCKKNIEDSLAILDEETDEELKEMAKEE
LNESKARVEELEHELKILLLPKDPNDDKDIVVEIRAGAGGEEAALFAAELFRMYVHYAQARGWKVEVLSGDETGIGGMKE
VEFMVKGQGAYSIMKYESGVHRVQRIPVTESNGRIQTSTASVAVMPEAEEVDIHIDEKDIRIDVMRASGNGGQCVNTTDS
AVRLTHYPTGIVIYSQTEKSQIQNKEKAFALLRAKLYDIETQKAHDAEADARRSQIGTGDRAEKIRTYNFPQGRVTDHRI
GLTLYKLDKIMDGDIQDIIDACIAADQAAKLAKMGED

Sequences:

>Translated_357_residues
MFDRLEDTVNRYEDITAELGNPDVVNDQEKFKKLMKEQSSLAPLIETYTEYKNCKKNIEDSLAILDEETDEELKEMAKEE
LNESKARVEELEHELKILLLPKDPNDDKDIVVEIRAGAGGEEAALFAAELFRMYVHYAQARGWKVEVLSGDETGIGGMKE
VEFMVKGQGAYSIMKYESGVHRVQRIPVTESNGRIQTSTASVAVMPEAEEVDIHIDEKDIRIDVMRASGNGGQCVNTTDS
AVRLTHYPTGIVIYSQTEKSQIQNKEKAFALLRAKLYDIETQKAHDAEADARRSQIGTGDRAEKIRTYNFPQGRVTDHRI
GLTLYKLDKIMDGDIQDIIDACIAADQAAKLAKMGED
>Mature_357_residues
MFDRLEDTVNRYEDITAELGNPDVVNDQEKFKKLMKEQSSLAPLIETYTEYKNCKKNIEDSLAILDEETDEELKEMAKEE
LNESKARVEELEHELKILLLPKDPNDDKDIVVEIRAGAGGEEAALFAAELFRMYVHYAQARGWKVEVLSGDETGIGGMKE
VEFMVKGQGAYSIMKYESGVHRVQRIPVTESNGRIQTSTASVAVMPEAEEVDIHIDEKDIRIDVMRASGNGGQCVNTTDS
AVRLTHYPTGIVIYSQTEKSQIQNKEKAFALLRAKLYDIETQKAHDAEADARRSQIGTGDRAEKIRTYNFPQGRVTDHRI
GLTLYKLDKIMDGDIQDIIDACIAADQAAKLAKMGED

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=265, Percent_Identity=47.5471698113208, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI34577120, Length=337, Percent_Identity=38.8724035608309, Blast_Score=238, Evalue=5e-63,
Organism=Homo sapiens, GI166795305, Length=180, Percent_Identity=45, Blast_Score=182, Evalue=4e-46,
Organism=Escherichia coli, GI1787462, Length=354, Percent_Identity=49.1525423728814, Blast_Score=343, Evalue=1e-95,
Organism=Escherichia coli, GI2367172, Length=342, Percent_Identity=38.3040935672515, Blast_Score=215, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI17542784, Length=265, Percent_Identity=40, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321295, Length=345, Percent_Identity=40.5797101449275, Blast_Score=239, Evalue=4e-64,
Organism=Drosophila melanogaster, GI19921226, Length=277, Percent_Identity=42.9602888086643, Blast_Score=237, Evalue=8e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_EUBE2 (C4Z1S4)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930882.1
- GeneID:   7957103
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01442
- OMA:   SEQGGYK
- ProtClustDB:   CLSK2506069
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40098; Mature: 40098

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDRLEDTVNRYEDITAELGNPDVVNDQEKFKKLMKEQSSLAPLIETYTEYKNCKKNIED
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAILDEETDEELKEMAKEELNESKARVEELEHELKILLLPKDPNDDKDIVVEIRAGAGG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEECCCCC
EEAALFAAELFRMYVHYAQARGWKVEVLSGDETGIGGMKEVEFMVKGQGAYSIMKYESGV
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHEEEEEECCCCEEEHHHHHHH
HRVQRIPVTESNGRIQTSTASVAVMPEAEEVDIHIDEKDIRIDVMRASGNGGQCVNTTDS
HHHHCCCCCCCCCEEEECCCEEEECCCCCCEEEEECCCCEEEEEEEECCCCCCEECCCCC
AVRLTHYPTGIVIYSQTEKSQIQNKEKAFALLRAKLYDIETQKAHDAEADARRSQIGTGD
EEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCC
RAEKIRTYNFPQGRVTDHRIGLTLYKLDKIMDGDIQDIIDACIAADQAAKLAKMGED
HHHHHEECCCCCCCCCCHHHCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFDRLEDTVNRYEDITAELGNPDVVNDQEKFKKLMKEQSSLAPLIETYTEYKNCKKNIED
CCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAILDEETDEELKEMAKEELNESKARVEELEHELKILLLPKDPNDDKDIVVEIRAGAGG
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEEEEEEECCCCC
EEAALFAAELFRMYVHYAQARGWKVEVLSGDETGIGGMKEVEFMVKGQGAYSIMKYESGV
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHEEEEEECCCCEEEHHHHHHH
HRVQRIPVTESNGRIQTSTASVAVMPEAEEVDIHIDEKDIRIDVMRASGNGGQCVNTTDS
HHHHCCCCCCCCCEEEECCCEEEECCCCCCEEEEECCCCEEEEEEEECCCCCCEECCCCC
AVRLTHYPTGIVIYSQTEKSQIQNKEKAFALLRAKLYDIETQKAHDAEADARRSQIGTGD
EEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCC
RAEKIRTYNFPQGRVTDHRIGLTLYKLDKIMDGDIQDIIDACIAADQAAKLAKMGED
HHHHHEECCCCCCCCCCHHHCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA