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The map label for this gene is nadB [H]

Identifier: 238917353

GI number: 238917353

Start: 1484064

End: 1485401

Strand: Reverse

Name: nadB [H]

Synonym: EUBELI_01430

Alternate gene names: 238917353

Gene position: 1485401-1484064 (Counterclockwise)

Preceding gene: 238917354

Following gene: 238917352

Centisome position: 69.28

GC content: 37.52

Gene sequence:

>1338_bases
ATGAATAATACAATTAAAACAGATGTGCTGATAGCCGGATGTGGCTGTTCTGGTCTGTATATGGCAATGAATCTGCCTGC
TGATAAGAAGATTCTTATGATAACAAAGTCTGATTGTGAGAGCAGTGATTCATTTCTGGCACAGGGCGGTATGTGTATGC
TAAAATGTGAAGAAGATTACGACAGTTATTTTGAAGATACAATGAAAGCCGGCCATTATGAAAATGATAAGAAATCTGTT
GAAATAATGATCCGTTCATCAGCAGATGTAGTAAAAGATCTTGTCGATTGTGGAGCAAGATTTGCAAGAGAGGCTGACGG
AAGTCTTGCATACACAAGAGAAGGAGCTCATTCATCTAACAGGATAATATTCCATGAAGATATTACAGGTAAAGAGATAA
CAAGTCATCTGTTAGAGAAGGTGAGAACTCTTCCCAATGTGACAATTATGGAATACACAAGACTGCTTGATATAATAAGC
AGAGATAACAGATGCCTTGGTGCGGTTATAAGGACAGAAAATGGAGAGCTTATTAAGGTAGAAGCTTCAGATGTTGTACT
TGCTACAGGTGGAATTGGTGGTTTATACAGACATTCAACGAATTTCAGACATCTTACAGGAGATGCAATTGCAATTGCTA
TAAAGCATGGAGTAGAATTAAAGGATATTAATTATGTCCAGATTCATCCAACAACATTTTATTCTGACAAAGAGGAAGAC
AGAAGCTTTCTTATATCTGAATCGGTAAGGGGTGAAGGCGCAAAGCTTTATGATAAGAATGGAAAAAGATTTGTTAATGA
GTTGCTTCCAAGAGACCTGCTTACAGAAGAGATTAGAAAACAGATGGCAAAGGACAATACAGACTTTGTATGGGAAGATT
TAAGGACAATACCTGAAGAAGAATTAAAAACGCATTTTCCTAATATAGTGCAGTATTGTATTGATAAGGGATATGACCCG
TCAAAAGAGTGTATTCCGGTTGTTCCTGCACAGCACTATTTCATGGGAGGAATTAAGGTAGACCATGAGAGCAGAACAAC
AATGGATAACCTGTATGCAATAGGTGAGACAGCCTGCAACGGAGTTCATGGAAGAAACAGGCTTGCAAGCAACTCACTTC
TTGAAAGTCTTGTGTTTGCAAAGAGGGCAGCACTTGATATTACTGAAAATGCAGTTGTAAAACCGACACAGGATGAGATT
ACATATTTTGATACATTTGATGCAGAAAAATATGCAGATGAGTCGAAAATTGACGGAGAATATGCAAAATTAGTCAATAA
AAAGATTGAAGAGGCAGATAAAGCCTATGAAGAAAGCCAGAAGAAGAATTACGCATAA

Upstream 100 bases:

>100_bases
TAGATGAAGAAGTTGCAAAGAAAGCAATGAAGCCACTTACAAGAATGCTTGAGCTTGCAAAATAAGATTGTAGTATGCGC
AGAAAGAATGGAGGTATCCT

Downstream 100 bases:

>100_bases
TATATAGAAATGGAGAAATGATATGTTTGATAAAGTTACTTTAAAGTTAAATGTGGATCCGCTCATTCTTGGAGCACTTA
AAGAAGATATTACAAGTGAG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MNNTIKTDVLIAGCGCSGLYMAMNLPADKKILMITKSDCESSDSFLAQGGMCMLKCEEDYDSYFEDTMKAGHYENDKKSV
EIMIRSSADVVKDLVDCGARFAREADGSLAYTREGAHSSNRIIFHEDITGKEITSHLLEKVRTLPNVTIMEYTRLLDIIS
RDNRCLGAVIRTENGELIKVEASDVVLATGGIGGLYRHSTNFRHLTGDAIAIAIKHGVELKDINYVQIHPTTFYSDKEED
RSFLISESVRGEGAKLYDKNGKRFVNELLPRDLLTEEIRKQMAKDNTDFVWEDLRTIPEEELKTHFPNIVQYCIDKGYDP
SKECIPVVPAQHYFMGGIKVDHESRTTMDNLYAIGETACNGVHGRNRLASNSLLESLVFAKRAALDITENAVVKPTQDEI
TYFDTFDAEKYADESKIDGEYAKLVNKKIEEADKAYEESQKKNYA

Sequences:

>Translated_445_residues
MNNTIKTDVLIAGCGCSGLYMAMNLPADKKILMITKSDCESSDSFLAQGGMCMLKCEEDYDSYFEDTMKAGHYENDKKSV
EIMIRSSADVVKDLVDCGARFAREADGSLAYTREGAHSSNRIIFHEDITGKEITSHLLEKVRTLPNVTIMEYTRLLDIIS
RDNRCLGAVIRTENGELIKVEASDVVLATGGIGGLYRHSTNFRHLTGDAIAIAIKHGVELKDINYVQIHPTTFYSDKEED
RSFLISESVRGEGAKLYDKNGKRFVNELLPRDLLTEEIRKQMAKDNTDFVWEDLRTIPEEELKTHFPNIVQYCIDKGYDP
SKECIPVVPAQHYFMGGIKVDHESRTTMDNLYAIGETACNGVHGRNRLASNSLLESLVFAKRAALDITENAVVKPTQDEI
TYFDTFDAEKYADESKIDGEYAKLVNKKIEEADKAYEESQKKNYA
>Mature_445_residues
MNNTIKTDVLIAGCGCSGLYMAMNLPADKKILMITKSDCESSDSFLAQGGMCMLKCEEDYDSYFEDTMKAGHYENDKKSV
EIMIRSSADVVKDLVDCGARFAREADGSLAYTREGAHSSNRIIFHEDITGKEITSHLLEKVRTLPNVTIMEYTRLLDIIS
RDNRCLGAVIRTENGELIKVEASDVVLATGGIGGLYRHSTNFRHLTGDAIAIAIKHGVELKDINYVQIHPTTFYSDKEED
RSFLISESVRGEGAKLYDKNGKRFVNELLPRDLLTEEIRKQMAKDNTDFVWEDLRTIPEEELKTHFPNIVQYCIDKGYDP
SKECIPVVPAQHYFMGGIKVDHESRTTMDNLYAIGETACNGVHGRNRLASNSLLESLVFAKRAALDITENAVVKPTQDEI
TYFDTFDAEKYADESKIDGEYAKLVNKKIEEADKAYEESQKKNYA

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=423, Percent_Identity=30.9692671394799, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1788928, Length=422, Percent_Identity=36.0189573459716, Blast_Score=261, Evalue=9e-71,
Organism=Escherichia coli, GI1790597, Length=397, Percent_Identity=32.7455919395466, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1786942, Length=394, Percent_Identity=30.7106598984772, Blast_Score=159, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17505833, Length=393, Percent_Identity=30.0254452926209, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17550100, Length=397, Percent_Identity=29.4710327455919, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI71986328, Length=429, Percent_Identity=28.4382284382284, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322416, Length=399, Percent_Identity=29.8245614035088, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322701, Length=399, Percent_Identity=29.0726817042606, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6322511, Length=480, Percent_Identity=27.5, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6320788, Length=479, Percent_Identity=26.0960334029228, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI17137288, Length=395, Percent_Identity=30.379746835443, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24655642, Length=395, Percent_Identity=30.379746835443, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24655647, Length=395, Percent_Identity=30.379746835443, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24663005, Length=407, Percent_Identity=30.2211302211302, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027 [H]

Pfam domain/function: PF00890 FAD_binding_2 [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 50067; Mature: 50067

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTIKTDVLIAGCGCSGLYMAMNLPADKKILMITKSDCESSDSFLAQGGMCMLKCEEDY
CCCCEEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHCCCEEEEEECCHH
DSYFEDTMKAGHYENDKKSVEIMIRSSADVVKDLVDCGARFAREADGSLAYTREGAHSSN
HHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
RIIFHEDITGKEITSHLLEKVRTLPNVTIMEYTRLLDIISRDNRCLGAVIRTENGELIKV
EEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEE
EASDVVLATGGIGGLYRHSTNFRHLTGDAIAIAIKHGVELKDINYVQIHPTTFYSDKEED
ECCCEEEEECCCCCHHHCCCCCEEECCCEEEEEEECCCEEECCCEEEEECCEECCCCHHH
RSFLISESVRGEGAKLYDKNGKRFVNELLPRDLLTEEIRKQMAKDNTDFVWEDLRTIPEE
HHEEEHHCCCCCCCEEECCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCHH
ELKTHFPNIVQYCIDKGYDPSKECIPVVPAQHYFMGGIKVDHESRTTMDNLYAIGETACN
HHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHEECCEEECCCCCCCHHHHHHHHHHHHC
GVHGRNRLASNSLLESLVFAKRAALDITENAVVKPTQDEITYFDTFDAEKYADESKIDGE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEECCCHHHHCCCCCCCHH
YAKLVNKKIEEADKAYEESQKKNYA
HHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNNTIKTDVLIAGCGCSGLYMAMNLPADKKILMITKSDCESSDSFLAQGGMCMLKCEEDY
CCCCEEEEEEEEECCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHCCCEEEEEECCHH
DSYFEDTMKAGHYENDKKSVEIMIRSSADVVKDLVDCGARFAREADGSLAYTREGAHSSN
HHHHHHHHHCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
RIIFHEDITGKEITSHLLEKVRTLPNVTIMEYTRLLDIISRDNRCLGAVIRTENGELIKV
EEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEE
EASDVVLATGGIGGLYRHSTNFRHLTGDAIAIAIKHGVELKDINYVQIHPTTFYSDKEED
ECCCEEEEECCCCCHHHCCCCCEEECCCEEEEEEECCCEEECCCEEEEECCEECCCCHHH
RSFLISESVRGEGAKLYDKNGKRFVNELLPRDLLTEEIRKQMAKDNTDFVWEDLRTIPEE
HHEEEHHCCCCCCCEEECCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCHH
ELKTHFPNIVQYCIDKGYDPSKECIPVVPAQHYFMGGIKVDHESRTTMDNLYAIGETACN
HHHHHHHHHHHHHHHCCCCCCCCCEECCCCCHHEECCEEECCCCCCCHHHHHHHHHHHHC
GVHGRNRLASNSLLESLVFAKRAALDITENAVVKPTQDEITYFDTFDAEKYADESKIDGE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCEEEEECCCHHHHCCCCCCCHH
YAKLVNKKIEEADKAYEESQKKNYA
HHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]