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The map label for this gene is speE

Identifier: 238917348

GI number: 238917348

Start: 1478572

End: 1479429

Strand: Reverse

Name: speE

Synonym: EUBELI_01425

Alternate gene names: 238917348

Gene position: 1479429-1478572 (Counterclockwise)

Preceding gene: 238917349

Following gene: 238917347

Centisome position: 69.0

GC content: 40.21

Gene sequence:

>858_bases
ATGGAATTTTGGTTTTCAGAACTTCATTCACCACATGTGAAGTTTGATATCAGGGTTGAAAAACAACTGTTTTCTGGAGA
GAGCGACTATCAGAGAATAGATGTATTTCAGTCGCCAGAGTTCGGAAAATTCCTGACACTTAATGGAAGTGTTATATTTT
CAGAACAGGATTGTTTCATCTATAACGAGATGGTGGTGCATGTGCCAATGTCAGTCCATCCAGATGTTAAAAATGTATTG
ATAATCGGTGGCGGTGACGGAGGTGTATCAAAGGAACTGTTGCAGTATGACAATATCGAAAATATTGACATAGTTGAACA
GGACAACATGTTTGTTGATGTGTGCAGGGAGTATTTTCCAGAGACGGCATCAGGATTAGAAGATGAGAGAGTGCACATTT
ATAATTCAGATGCATTAAGGTTCTTACGCTCCAAGCAGGATATGTATGACCTTATAATTAATGACGCAACAGACCCGTTT
GGTGCGAGTGAGGGACTGTTTACAAGGGAATTCTATGGTAACTGTTATAAGGCACTTAAGGAGGATGGAATACTTGTGTA
CCAGCATGGAAGTCCTTTCTATGATGAAGATGAGGATGCATGCAGAAGCATGCACAGGAAGGTGTTCCACACATTTCCAA
TAAGCCGCGTGTATCAGGCACATATACCAACAAGTCCGTCAGGATACTGGCTGTTTGGATTTGCGTCAAAGAAATATCAT
CCGCTTAAGGATTTCAAGCCGGCAGAGTGGAAGAGAAGAAATATACATACAGAATATTACACAACTAATCTTCATACAGG
TGCATTCATGCTGCCAAAGTATGTTGAAGAATTATTAGAACAGGAGGAAGAAAGATGA

Upstream 100 bases:

>100_bases
TGCAGGGAATGGAAGATTCAACATTGCAGACACTGAATGTATTAAGAATGTAAATGTCCGATTTGTTCAATCGGTCAAAT
ATAGAAGGGAGAAAGTTTCT

Downstream 100 bases:

>100_bases
GCAGAGTATTAGTAATTGGTTGCGGAGGAGTTGCTTCAGTAGCAATCCACAAGATATGTCAGGTGAGCGAGGTGTTTTCA
GACCTGTGCATAGCGAGCAG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MEFWFSELHSPHVKFDIRVEKQLFSGESDYQRIDVFQSPEFGKFLTLNGSVIFSEQDCFIYNEMVVHVPMSVHPDVKNVL
IIGGGDGGVSKELLQYDNIENIDIVEQDNMFVDVCREYFPETASGLEDERVHIYNSDALRFLRSKQDMYDLIINDATDPF
GASEGLFTREFYGNCYKALKEDGILVYQHGSPFYDEDEDACRSMHRKVFHTFPISRVYQAHIPTSPSGYWLFGFASKKYH
PLKDFKPAEWKRRNIHTEYYTTNLHTGAFMLPKYVEELLEQEEER

Sequences:

>Translated_285_residues
MEFWFSELHSPHVKFDIRVEKQLFSGESDYQRIDVFQSPEFGKFLTLNGSVIFSEQDCFIYNEMVVHVPMSVHPDVKNVL
IIGGGDGGVSKELLQYDNIENIDIVEQDNMFVDVCREYFPETASGLEDERVHIYNSDALRFLRSKQDMYDLIINDATDPF
GASEGLFTREFYGNCYKALKEDGILVYQHGSPFYDEDEDACRSMHRKVFHTFPISRVYQAHIPTSPSGYWLFGFASKKYH
PLKDFKPAEWKRRNIHTEYYTTNLHTGAFMLPKYVEELLEQEEER
>Mature_285_residues
MEFWFSELHSPHVKFDIRVEKQLFSGESDYQRIDVFQSPEFGKFLTLNGSVIFSEQDCFIYNEMVVHVPMSVHPDVKNVL
IIGGGDGGVSKELLQYDNIENIDIVEQDNMFVDVCREYFPETASGLEDERVHIYNSDALRFLRSKQDMYDLIINDATDPF
GASEGLFTREFYGNCYKALKEDGILVYQHGSPFYDEDEDACRSMHRKVFHTFPISRVYQAHIPTSPSGYWLFGFASKKYH
PLKDFKPAEWKRRNIHTEYYTTNLHTGAFMLPKYVEELLEQEEER

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=285, Percent_Identity=32.9824561403509, Blast_Score=168, Evalue=6e-42,
Organism=Escherichia coli, GI1786312, Length=283, Percent_Identity=35.3356890459364, Blast_Score=183, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25154106, Length=276, Percent_Identity=35.1449275362319, Blast_Score=153, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI212645616, Length=231, Percent_Identity=36.3636363636364, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6325326, Length=292, Percent_Identity=32.5342465753425, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323175, Length=288, Percent_Identity=28.8194444444444, Blast_Score=138, Evalue=8e-34,
Organism=Drosophila melanogaster, GI24645443, Length=285, Percent_Identity=30.1754385964912, Blast_Score=162, Evalue=3e-40,
Organism=Drosophila melanogaster, GI21355941, Length=222, Percent_Identity=30.1801801801802, Blast_Score=127, Evalue=6e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_EUBE2 (C4Z1Q7)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002930865.1
- ProteinModelPortal:   C4Z1Q7
- GeneID:   7957086
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_01425
- OMA:   VKHPLVE
- ProtClustDB:   PRK00811
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 33361; Mature: 33361

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 31-31 BINDING 86-86 BINDING 106-106 BINDING 155-155 BINDING 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFWFSELHSPHVKFDIRVEKQLFSGESDYQRIDVFQSPEFGKFLTLNGSVIFSEQDCFI
CCCCHHHHCCCCEEEEEEEEHHHHCCCCCCEEEECCCCCCCCCEEEECCEEEEECCCCEE
YNEMVVHVPMSVHPDVKNVLIIGGGDGGVSKELLQYDNIENIDIVEQDNMFVDVCREYFP
EEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCC
ETASGLEDERVHIYNSDALRFLRSKQDMYDLIINDATDPFGASEGLFTREFYGNCYKALK
HHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
EDGILVYQHGSPFYDEDEDACRSMHRKVFHTFPISRVYQAHIPTSPSGYWLFGFASKKYH
HCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHEECCCCCCCCEEEEEECCCCCC
PLKDFKPAEWKRRNIHTEYYTTNLHTGAFMLPKYVEELLEQEEER
CCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEFWFSELHSPHVKFDIRVEKQLFSGESDYQRIDVFQSPEFGKFLTLNGSVIFSEQDCFI
CCCCHHHHCCCCEEEEEEEEHHHHCCCCCCEEEECCCCCCCCCEEEECCEEEEECCCCEE
YNEMVVHVPMSVHPDVKNVLIIGGGDGGVSKELLQYDNIENIDIVEQDNMFVDVCREYFP
EEEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCC
ETASGLEDERVHIYNSDALRFLRSKQDMYDLIINDATDPFGASEGLFTREFYGNCYKALK
HHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
EDGILVYQHGSPFYDEDEDACRSMHRKVFHTFPISRVYQAHIPTSPSGYWLFGFASKKYH
HCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHEECCCCCCCCEEEEEECCCCCC
PLKDFKPAEWKRRNIHTEYYTTNLHTGAFMLPKYVEELLEQEEER
CCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA