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The map label for this gene is clpB [H]

Identifier: 238917187

GI number: 238917187

Start: 1290796

End: 1293393

Strand: Reverse

Name: clpB [H]

Synonym: EUBELI_01260

Alternate gene names: 238917187

Gene position: 1293393-1290796 (Counterclockwise)

Preceding gene: 238917188

Following gene: 238917185

Centisome position: 60.32

GC content: 39.8

Gene sequence:

>2598_bases
ATGAACATTAATAAGTTTACACAGAAGTCTATTGAGGCTGTTAACCAGTGCGAGAAGATTGCCTACGATTATGGTAATCA
GGAGATTGATCAGGAACATTTTCTGTACAGTCTCTTAACTATAGAAGATAGTCTTATCGCGAGCCTGATAGAGAAGATGG
GAATTGATAAGGACACATTTATTAAGAATATTGAGACTTTACTTAGCCAGAAGAATAAGGTTTCAGGAAATGTACAGCTT
TATGTAAGCAATGATCTTAATAAGGTTTTAGTAAATGCTGAAGATGAAGCAAAGAGAATGGGGGATGCATATGTTTCTGT
TGAGCATCTTATGCTTGCGATGATTGCAGCACCTAACAAGGGTATAAAGCAGCTTTTTAAGACATATGGAATTAACAGGG
AATCATTCCTGTCAGCCCTTGCGACAGTAAGAGGTAACCAGAGTGTTACTTCGGATAATCCGGAGGCAACTTATGATACT
CTTAGCAAATATGCTACAGACCTTGTAGAAAGAGCCAAGGATGGAAAACTTGATCCGGTTATCGGAAGAGATAATGAAAT
AAGAAATGTAATAAGAATTCTTTCCCGTAAGACTAAGAACAATCCGGTTCTTATTGGTGAGCCAGGTGTTGGTAAAACAG
CAGTTGTTGAAGGACTTGCACAGAGAATTGTAAGAGGTGATGTTCCAGAAGGCTTAAAGGATAAGAAGCTTTTTGCACTG
GATATGGGCGCACTTGTTGCAGGTGCAAAGTATCGTGGTGAATTCGAGGAAAGACTTAAGGCTGTACTTGACGAGGTTAA
GAAATCTGAAGGACAGATAATTCTGTTTATTGATGAAATTCACACTATTGTCGGTGCAGGTAAGACAGAAGGTGCTATGG
ACGCAGGTAATATGTTAAAGCCAATGCTTGCCCGTGGTGAACTTCATTGTATAGGTGCTACAACTCTTGATGAGCACAGA
GAGTATATTGAGAAAGACCCGGCACTTGAAAGACGATTCCAGCCGGTTATGGTTGATGAGCCTACAGTTGAGGATACTAT
CTCAATCCTTAGAGGTCTTAAGGACAGATATGAAGTGTTCCATGGAGTTAAGATTACAGATGGCGCACTTGTAAGTGCAG
CTACTTTGTCAGACAGATATATTTCAGACAGATTCCTGCCAGATAAGGCTATTGACCTTGTAGATGAGGCATGTGCAATG
ATTAAGACTGAACTTGATTCAATGCCTGCCGAACTTGATGAGAAGAGAAGAAAGATTATGCAGCTTGAGATTGAGGAGGC
TGCTCTTAAGAAAGAGACAGATAACCTCAGTAAGGAAAGACTTGATGCATTACAGAAGGAATTATCTGAATTAAGGTCTG
ATTTCAATATCCAGAAAGCTAAATGGGACAGCGAGAAATCTTCTGTTGACCAGGTAAGCAAGTTAAAGGAAGAGATTGAC
CAGATTAATACCCAGATAGCACAGGCTAAGAGAGAATATGATCTTAATAAGGCAGCAGAACTTCAGTATGGTAAACTTCC
ACAGGCACAGAAGGCTTTGGAAGAAGCTGAGAAGAAGTCAAGTAATAGAGATATGAGTCTTGTTCATGAAAGTGTTACTG
AGGAAGAGATTGCCAAGATTATATCAAGATGGACTGGTATTCCGGTTAGCAAGTTAAGTGAGTCTGAAAGACAGAAAACT
CTTAATCTTGATGAACAGCTCCACAAGAGAGTTATCGGTCAGGATGAAGGTGTTACTAAGGTTACAGAGGCAATCATAAG
GTCTAAGGCTGGTATTAAAGACCCGACTAAGCCAATCGGTTCATTCCTGTTCTTAGGACCTACAGGTGTAGGTAAGACAG
AGCTTGCAAAGGCACTTGCTGAGAGTCTGTTTGATGATGAATCTAATATAGTAAGACTTGATATGTCCGAGTATATGGAG
AAGTATTCAGTATCCAGACTTATCGGAGCGCCTCCAGGATATGTAGGTTATGATGAAGGTGGACAGCTTACAGAAGCAGT
AAGAAGAAAACCATATTCAGTTGTTCTTTTTGATGAAGTTGAGAAAGCACATCCAGATGTATTTAATGTCCTCTTACAGG
TACTTGATGATGGCCGTATCACTGATTCACAGGGAAGAACTGTGGATTTTAAGAACACTATAATCATAATGACAAGTAAC
ATTGGTTCTTCATATCTGCTTGAAGGCATCAATCCTGATGGCTCAATTAAGCCAGAGGCAGAGGAAGCTGTTATGGGTGA
TTTAAAGAATCATTTCAGACCTGAATTCCTTAATCGTCTTGATGAGATAATTATGTTCAAGCCTCTTACTAAGGATAATA
TCAGCAATATTATCAATCTTCTTGTTGCAGATGTTAACAAGAGACTTGCAGATAAAGAGCTTGAGATTGTTCTTACAGAT
GCAGCCAAGGATTTCATAGTTGAAAACGGATTTGACCCAATGTATGGTGCAAGACCGCTTAAGAGATATGTCCAGAAGAC
AGTTGAGACACTGGCAGCCAAGTTAATACTTGCAGGCAATATCAATACAGGGGATGATATTGTTATTGACCTTGTAGATG
GAAAGCTGACAGCCAGAGCCGCTGCAAGACAGAAATAA

Upstream 100 bases:

>100_bases
TGACAGTGCTAACAATGAGATGTATAACATCAATTGTAAGAGGAAAACAATAGATAAACAGTGAAGTTAAGCATTCATTG
TAATAGAAGGAGGTAATCGT

Downstream 100 bases:

>100_bases
TAAGTAATTGATATAGCAGGTGTAATGTGCAGAGTGAGTCTGTATATTATACCTGCTTTTTAAACAAACTGGTTCTTTTC
ACTATTATATTCGTAATTAA

Product: ATP-dependent Clp protease ATP-binding subunit ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 865; Mature: 865

Protein sequence:

>865_residues
MNINKFTQKSIEAVNQCEKIAYDYGNQEIDQEHFLYSLLTIEDSLIASLIEKMGIDKDTFIKNIETLLSQKNKVSGNVQL
YVSNDLNKVLVNAEDEAKRMGDAYVSVEHLMLAMIAAPNKGIKQLFKTYGINRESFLSALATVRGNQSVTSDNPEATYDT
LSKYATDLVERAKDGKLDPVIGRDNEIRNVIRILSRKTKNNPVLIGEPGVGKTAVVEGLAQRIVRGDVPEGLKDKKLFAL
DMGALVAGAKYRGEFEERLKAVLDEVKKSEGQIILFIDEIHTIVGAGKTEGAMDAGNMLKPMLARGELHCIGATTLDEHR
EYIEKDPALERRFQPVMVDEPTVEDTISILRGLKDRYEVFHGVKITDGALVSAATLSDRYISDRFLPDKAIDLVDEACAM
IKTELDSMPAELDEKRRKIMQLEIEEAALKKETDNLSKERLDALQKELSELRSDFNIQKAKWDSEKSSVDQVSKLKEEID
QINTQIAQAKREYDLNKAAELQYGKLPQAQKALEEAEKKSSNRDMSLVHESVTEEEIAKIISRWTGIPVSKLSESERQKT
LNLDEQLHKRVIGQDEGVTKVTEAIIRSKAGIKDPTKPIGSFLFLGPTGVGKTELAKALAESLFDDESNIVRLDMSEYME
KYSVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLFDEVEKAHPDVFNVLLQVLDDGRITDSQGRTVDFKNTIIIMTSN
IGSSYLLEGINPDGSIKPEAEEAVMGDLKNHFRPEFLNRLDEIIMFKPLTKDNISNIINLLVADVNKRLADKELEIVLTD
AAKDFIVENGFDPMYGARPLKRYVQKTVETLAAKLILAGNINTGDDIVIDLVDGKLTARAAARQK

Sequences:

>Translated_865_residues
MNINKFTQKSIEAVNQCEKIAYDYGNQEIDQEHFLYSLLTIEDSLIASLIEKMGIDKDTFIKNIETLLSQKNKVSGNVQL
YVSNDLNKVLVNAEDEAKRMGDAYVSVEHLMLAMIAAPNKGIKQLFKTYGINRESFLSALATVRGNQSVTSDNPEATYDT
LSKYATDLVERAKDGKLDPVIGRDNEIRNVIRILSRKTKNNPVLIGEPGVGKTAVVEGLAQRIVRGDVPEGLKDKKLFAL
DMGALVAGAKYRGEFEERLKAVLDEVKKSEGQIILFIDEIHTIVGAGKTEGAMDAGNMLKPMLARGELHCIGATTLDEHR
EYIEKDPALERRFQPVMVDEPTVEDTISILRGLKDRYEVFHGVKITDGALVSAATLSDRYISDRFLPDKAIDLVDEACAM
IKTELDSMPAELDEKRRKIMQLEIEEAALKKETDNLSKERLDALQKELSELRSDFNIQKAKWDSEKSSVDQVSKLKEEID
QINTQIAQAKREYDLNKAAELQYGKLPQAQKALEEAEKKSSNRDMSLVHESVTEEEIAKIISRWTGIPVSKLSESERQKT
LNLDEQLHKRVIGQDEGVTKVTEAIIRSKAGIKDPTKPIGSFLFLGPTGVGKTELAKALAESLFDDESNIVRLDMSEYME
KYSVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLFDEVEKAHPDVFNVLLQVLDDGRITDSQGRTVDFKNTIIIMTSN
IGSSYLLEGINPDGSIKPEAEEAVMGDLKNHFRPEFLNRLDEIIMFKPLTKDNISNIINLLVADVNKRLADKELEIVLTD
AAKDFIVENGFDPMYGARPLKRYVQKTVETLAAKLILAGNINTGDDIVIDLVDGKLTARAAARQK
>Mature_865_residues
MNINKFTQKSIEAVNQCEKIAYDYGNQEIDQEHFLYSLLTIEDSLIASLIEKMGIDKDTFIKNIETLLSQKNKVSGNVQL
YVSNDLNKVLVNAEDEAKRMGDAYVSVEHLMLAMIAAPNKGIKQLFKTYGINRESFLSALATVRGNQSVTSDNPEATYDT
LSKYATDLVERAKDGKLDPVIGRDNEIRNVIRILSRKTKNNPVLIGEPGVGKTAVVEGLAQRIVRGDVPEGLKDKKLFAL
DMGALVAGAKYRGEFEERLKAVLDEVKKSEGQIILFIDEIHTIVGAGKTEGAMDAGNMLKPMLARGELHCIGATTLDEHR
EYIEKDPALERRFQPVMVDEPTVEDTISILRGLKDRYEVFHGVKITDGALVSAATLSDRYISDRFLPDKAIDLVDEACAM
IKTELDSMPAELDEKRRKIMQLEIEEAALKKETDNLSKERLDALQKELSELRSDFNIQKAKWDSEKSSVDQVSKLKEEID
QINTQIAQAKREYDLNKAAELQYGKLPQAQKALEEAEKKSSNRDMSLVHESVTEEEIAKIISRWTGIPVSKLSESERQKT
LNLDEQLHKRVIGQDEGVTKVTEAIIRSKAGIKDPTKPIGSFLFLGPTGVGKTELAKALAESLFDDESNIVRLDMSEYME
KYSVSRLIGAPPGYVGYDEGGQLTEAVRRKPYSVVLFDEVEKAHPDVFNVLLQVLDDGRITDSQGRTVDFKNTIIIMTSN
IGSSYLLEGINPDGSIKPEAEEAVMGDLKNHFRPEFLNRLDEIIMFKPLTKDNISNIINLLVADVNKRLADKELEIVLTD
AAKDFIVENGFDPMYGARPLKRYVQKTVETLAAKLILAGNINTGDDIVIDLVDGKLTARAAARQK

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=299, Percent_Identity=38.1270903010033, Blast_Score=200, Evalue=5e-51,
Organism=Escherichia coli, GI1788943, Length=863, Percent_Identity=53.5341830822711, Blast_Score=919, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=347, Percent_Identity=40.0576368876081, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6320464, Length=706, Percent_Identity=52.9745042492918, Blast_Score=775, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=867, Percent_Identity=41.522491349481, Blast_Score=672, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96473; Mature: 96473

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNINKFTQKSIEAVNQCEKIAYDYGNQEIDQEHFLYSLLTIEDSLIASLIEKMGIDKDTF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IKNIETLLSQKNKVSGNVQLYVSNDLNKVLVNAEDEAKRMGDAYVSVEHLMLAMIAAPNK
HHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCH
GIKQLFKTYGINRESFLSALATVRGNQSVTSDNPEATYDTLSKYATDLVERAKDGKLDPV
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
IGRDNEIRNVIRILSRKTKNNPVLIGEPGVGKTAVVEGLAQRIVRGDVPEGLKDKKLFAL
CCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
DMGALVAGAKYRGEFEERLKAVLDEVKKSEGQIILFIDEIHTIVGAGKTEGAMDAGNMLK
EHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHH
PMLARGELHCIGATTLDEHREYIEKDPALERRFQPVMVDEPTVEDTISILRGLKDRYEVF
HHHHCCCEEEEECCCHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HGVKITDGALVSAATLSDRYISDRFLPDKAIDLVDEACAMIKTELDSMPAELDEKRRKIM
CCCEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QLEIEEAALKKETDNLSKERLDALQKELSELRSDFNIQKAKWDSEKSSVDQVSKLKEEID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHH
QINTQIAQAKREYDLNKAAELQYGKLPQAQKALEEAEKKSSNRDMSLVHESVTEEEIAKI
HHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHH
ISRWTGIPVSKLSESERQKTLNLDEQLHKRVIGQDEGVTKVTEAIIRSKAGIKDPTKPIG
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHC
SFLFLGPTGVGKTELAKALAESLFDDESNIVRLDMSEYMEKYSVSRLIGAPPGYVGYDEG
CEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCC
GQLTEAVRRKPYSVVLFDEVEKAHPDVFNVLLQVLDDGRITDSQGRTVDFKNTIIIMTSN
CHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECC
IGSSYLLEGINPDGSIKPEAEEAVMGDLKNHFRPEFLNRLDEIIMFKPLTKDNISNIINL
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
LVADVNKRLADKELEIVLTDAAKDFIVENGFDPMYGARPLKRYVQKTVETLAAKLILAGN
HHHHHHHHHCCCCCEEEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECC
INTGDDIVIDLVDGKLTARAAARQK
CCCCCCEEEEEECCCHHHHHHHCCC
>Mature Secondary Structure
MNINKFTQKSIEAVNQCEKIAYDYGNQEIDQEHFLYSLLTIEDSLIASLIEKMGIDKDTF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IKNIETLLSQKNKVSGNVQLYVSNDLNKVLVNAEDEAKRMGDAYVSVEHLMLAMIAAPNK
HHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCH
GIKQLFKTYGINRESFLSALATVRGNQSVTSDNPEATYDTLSKYATDLVERAKDGKLDPV
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
IGRDNEIRNVIRILSRKTKNNPVLIGEPGVGKTAVVEGLAQRIVRGDVPEGLKDKKLFAL
CCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
DMGALVAGAKYRGEFEERLKAVLDEVKKSEGQIILFIDEIHTIVGAGKTEGAMDAGNMLK
EHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHH
PMLARGELHCIGATTLDEHREYIEKDPALERRFQPVMVDEPTVEDTISILRGLKDRYEVF
HHHHCCCEEEEECCCHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HGVKITDGALVSAATLSDRYISDRFLPDKAIDLVDEACAMIKTELDSMPAELDEKRRKIM
CCCEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QLEIEEAALKKETDNLSKERLDALQKELSELRSDFNIQKAKWDSEKSSVDQVSKLKEEID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHH
QINTQIAQAKREYDLNKAAELQYGKLPQAQKALEEAEKKSSNRDMSLVHESVTEEEIAKI
HHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHH
ISRWTGIPVSKLSESERQKTLNLDEQLHKRVIGQDEGVTKVTEAIIRSKAGIKDPTKPIG
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHC
SFLFLGPTGVGKTELAKALAESLFDDESNIVRLDMSEYMEKYSVSRLIGAPPGYVGYDEG
CEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCC
GQLTEAVRRKPYSVVLFDEVEKAHPDVFNVLLQVLDDGRITDSQGRTVDFKNTIIIMTSN
CHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECC
IGSSYLLEGINPDGSIKPEAEEAVMGDLKNHFRPEFLNRLDEIIMFKPLTKDNISNIINL
CCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
LVADVNKRLADKELEIVLTDAAKDFIVENGFDPMYGARPLKRYVQKTVETLAAKLILAGN
HHHHHHHHHCCCCCEEEEECHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECC
INTGDDIVIDLVDGKLTARAAARQK
CCCCCCEEEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]