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The map label for this gene is ppdK [H]

Identifier: 238917185

GI number: 238917185

Start: 1287701

End: 1290331

Strand: Reverse

Name: ppdK [H]

Synonym: EUBELI_01258

Alternate gene names: 238917185

Gene position: 1290331-1287701 (Counterclockwise)

Preceding gene: 238917187

Following gene: 238917184

Centisome position: 60.18

GC content: 41.81

Gene sequence:

>2631_bases
ATGGGAAAGAAATGGGTGTATTTGTTTACAGAGGGTAACGCAGATATGCGTGAGCTTCTCGGAGGTAAAGGTGCTAACTT
GGCAGAGATGACTAATATCGGTCTACCTGTACCACAGGGATTCACAATTACAACAGAAGCTTGTACCCAGTATTACGAGG
ATGGTAGAGAGATCAATAATGAGATCATGGGACAGATTAACGAACATATTGAGAAGATGGAACAGATTACAGGCAAGAAG
TTTGGAGATATGGAGAATCCACTTTTAGTTTCTGTTCGTTCTGGAGCGAGAGCTTCTATGCCGGGTATGATGGATACTAT
TCTTAATCTTGGACTTAATGAGGATGTTGTTAATGTAATTGCACAGAAATCCGGAAATCCAAGATGGGCATGGGATTGTT
ATAGAAGATTTATCCAGATGTATTCAGATGTAGTTATGGAAGTTGGTAAGAAATATTTCGAAGAGCTTATTGACAAGATG
AAGGATGAGAGAGGGGTTAAGCTTGATGTAGAGCTTACAGCGGATGATCTTAAGGAGCTTGCAACACAGTTTAAGGCTGA
ATATAAGTCTAAGATTGGCAAGGATTTCCCAGATGATCCTAAGGAACAGTTATACGGAGCTATAAAAGCTGTATTCCGTT
CATGGGACAACCCAAGAGCTAATGTATACAGAAGAGATAATGATATTCCTTATTCTTGGGGAACAGCAGTTAATGTACAG
TCTATGGCATTTGGTAATATGGGGGATGACTGTGGAACTGGTGTTGCATTTACAAGAGATCCTGCAACTGGTGCTAAAGG
ACTTTTTGGAGAGTTCCTTACTAACGCACAGGGTGAAGATGTTGTTGCCGGAGTAAGAACTCCAATGCATATTCAGGAAA
TGAGTGAGAAGTTCCCGGAAGCATTTGAGCAGTTTAAGGATGTGTGTGCAACTCTGGAAGAACATTACAGAGATATGCAG
GATATGGAATTTACAGTAGAGCATGGCAAGCTGTTCATGTTACAGACACGTAATGGTAAGAGAACAGCACAGGCAGCATT
AAAGATTGCATGCGATTTTGTAGATGAGGGGATGAGAACAGAGAAGGAAGCAGTTGCAATGATTGATCCTCGTAATCTTG
ATACACTTCTTCATCCTCAGTTTGATGCAGCTGCACTTAAGGCTGCAACTCCAGCAGGAAGAGGTCTTGGAGCATCTCCT
GGCGCTGCATGTGGTAAGATTGTTTTCTCAGCAGAAGACGCAGAAGAGTGGAATGCAAGAGGAGAGAAGGTTGTTCTTGT
AAGACTTGAGACATCGCCAGAAGATATTACAGGTATGAAGGCTTCACAGGGTATTCTTACTGTAAGAGGAGGTATGACAA
GCCATGCAGCGGTTGTTGCAAGAGGTATGGGAACCTGCTGTGTATCTGGCTGTGGTGATATTGCAATGGATGAAGCTAAT
AAGAAGTTCACACTTGCAGGCAAGGAATACCATGAAGGAGATTATATATCTATTGATGGTACAACAGGCAACATATATGA
TGGACAGATTCCTACGGTTGATGCCAAGATTGCAGGAGAGTTTGGAAGAGTTATGGAGTGGGCTGATAAGTACAGAAAGT
TAAAGGTTCGTACTAATGCAGATACTCCTAAGGATGCCAAGAAGGCAAGAGAACTTGGAGCAGAAGGTATCGGACTTTGC
CGTACTGAGCATATGTTCTTTGAAGAGGACAGAATTGCAGCATTCAGAGAGATGATATGTTCTGATACTGTAGAAGAAAG
AGAAGCAGCACTGGATAAGATTCTGCCATATCAGCAGGGAGATTTCGAAGCACTTTATGAGGCACTTGAAGGTAATCCTG
TAACTATCAGATTCCTAGATCCACCTCTTCATGAATTTGTTCCTACAGAGGAAGAAGATATTAAGAAGTTAGCTGAATCA
CAGGGCAAGTCAGTAGAGACTATTAAGAATATTATTGCAAGTCTTCATGAGTTTAATCCAATGATGGGACACAGAGGATG
CCGTCTTGCAGTAACATATCCAGAGATTGCCAAGATGCAGACAAGAGCTGTTATAAGAGCTGCAATCAATGTTAAGAAGG
TACATCCTGACTGGAATGTTAAGCCGGAGATTATGATTCCGCTTATCTGTGAGGTTAAGGAATTAAAGTATGTAAAGAAG
ACTGTTGTTGAGACAGCAGATGAAGAGATTAAGGCAGCAGGAATCGACCTTGAATACGAGGTTGGTACTATGATTGAGAT
TCCAAGAGCTGCACTTACTGCTGATGAGATTGCTAAGGAAGCTGATTTCTTCTGCTTTGGTACTAACGATCTTACACAGA
TGACATTCGGATTCTCAAGAGATGACGCTGGTAAGTTCCTTAATGCATACTATGAATCTAAGATATTTGAGAATGACCCA
TTTGCAAAGCTTGACCAGAATGGTGTAGGCAAGCTTATGGAGATGACAATAAAGCTTGGAAGACCGGTTAATCCGAATCT
TCACATAGGTATCTGTGGAGAGCATGGAGGAGATCCTTCATCAGTTGCATTCTGCCACAAGATTGGGCTTGATTATGTGA
GCTGCTCACCATTCAGAGTACCTATTGCAAGACTTGCAGCAGCACAGGCTGCTATTGCAGAAGAGAAATAA

Upstream 100 bases:

>100_bases
ACATTTATGGTTGCACGAAAACGATTACTATGTTATAATTTTAACAAGCTAAGAAATATACTACTTAAATAACTGAATAT
TAAATTATGGAGGTTTTGTT

Downstream 100 bases:

>100_bases
TCCAATTTCTGACCATAACACGAGTTTCGGGGCATGGAAAGCAAAAGCTTTTCATGCCCCGAAATGTTTAAAATGGAATA
TTTTTTCATTGCCCTAAATC

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 876; Mature: 875

Protein sequence:

>876_residues
MGKKWVYLFTEGNADMRELLGGKGANLAEMTNIGLPVPQGFTITTEACTQYYEDGREINNEIMGQINEHIEKMEQITGKK
FGDMENPLLVSVRSGARASMPGMMDTILNLGLNEDVVNVIAQKSGNPRWAWDCYRRFIQMYSDVVMEVGKKYFEELIDKM
KDERGVKLDVELTADDLKELATQFKAEYKSKIGKDFPDDPKEQLYGAIKAVFRSWDNPRANVYRRDNDIPYSWGTAVNVQ
SMAFGNMGDDCGTGVAFTRDPATGAKGLFGEFLTNAQGEDVVAGVRTPMHIQEMSEKFPEAFEQFKDVCATLEEHYRDMQ
DMEFTVEHGKLFMLQTRNGKRTAQAALKIACDFVDEGMRTEKEAVAMIDPRNLDTLLHPQFDAAALKAATPAGRGLGASP
GAACGKIVFSAEDAEEWNARGEKVVLVRLETSPEDITGMKASQGILTVRGGMTSHAAVVARGMGTCCVSGCGDIAMDEAN
KKFTLAGKEYHEGDYISIDGTTGNIYDGQIPTVDAKIAGEFGRVMEWADKYRKLKVRTNADTPKDAKKARELGAEGIGLC
RTEHMFFEEDRIAAFREMICSDTVEEREAALDKILPYQQGDFEALYEALEGNPVTIRFLDPPLHEFVPTEEEDIKKLAES
QGKSVETIKNIIASLHEFNPMMGHRGCRLAVTYPEIAKMQTRAVIRAAINVKKVHPDWNVKPEIMIPLICEVKELKYVKK
TVVETADEEIKAAGIDLEYEVGTMIEIPRAALTADEIAKEADFFCFGTNDLTQMTFGFSRDDAGKFLNAYYESKIFENDP
FAKLDQNGVGKLMEMTIKLGRPVNPNLHIGICGEHGGDPSSVAFCHKIGLDYVSCSPFRVPIARLAAAQAAIAEEK

Sequences:

>Translated_876_residues
MGKKWVYLFTEGNADMRELLGGKGANLAEMTNIGLPVPQGFTITTEACTQYYEDGREINNEIMGQINEHIEKMEQITGKK
FGDMENPLLVSVRSGARASMPGMMDTILNLGLNEDVVNVIAQKSGNPRWAWDCYRRFIQMYSDVVMEVGKKYFEELIDKM
KDERGVKLDVELTADDLKELATQFKAEYKSKIGKDFPDDPKEQLYGAIKAVFRSWDNPRANVYRRDNDIPYSWGTAVNVQ
SMAFGNMGDDCGTGVAFTRDPATGAKGLFGEFLTNAQGEDVVAGVRTPMHIQEMSEKFPEAFEQFKDVCATLEEHYRDMQ
DMEFTVEHGKLFMLQTRNGKRTAQAALKIACDFVDEGMRTEKEAVAMIDPRNLDTLLHPQFDAAALKAATPAGRGLGASP
GAACGKIVFSAEDAEEWNARGEKVVLVRLETSPEDITGMKASQGILTVRGGMTSHAAVVARGMGTCCVSGCGDIAMDEAN
KKFTLAGKEYHEGDYISIDGTTGNIYDGQIPTVDAKIAGEFGRVMEWADKYRKLKVRTNADTPKDAKKARELGAEGIGLC
RTEHMFFEEDRIAAFREMICSDTVEEREAALDKILPYQQGDFEALYEALEGNPVTIRFLDPPLHEFVPTEEEDIKKLAES
QGKSVETIKNIIASLHEFNPMMGHRGCRLAVTYPEIAKMQTRAVIRAAINVKKVHPDWNVKPEIMIPLICEVKELKYVKK
TVVETADEEIKAAGIDLEYEVGTMIEIPRAALTADEIAKEADFFCFGTNDLTQMTFGFSRDDAGKFLNAYYESKIFENDP
FAKLDQNGVGKLMEMTIKLGRPVNPNLHIGICGEHGGDPSSVAFCHKIGLDYVSCSPFRVPIARLAAAQAAIAEEK
>Mature_875_residues
GKKWVYLFTEGNADMRELLGGKGANLAEMTNIGLPVPQGFTITTEACTQYYEDGREINNEIMGQINEHIEKMEQITGKKF
GDMENPLLVSVRSGARASMPGMMDTILNLGLNEDVVNVIAQKSGNPRWAWDCYRRFIQMYSDVVMEVGKKYFEELIDKMK
DERGVKLDVELTADDLKELATQFKAEYKSKIGKDFPDDPKEQLYGAIKAVFRSWDNPRANVYRRDNDIPYSWGTAVNVQS
MAFGNMGDDCGTGVAFTRDPATGAKGLFGEFLTNAQGEDVVAGVRTPMHIQEMSEKFPEAFEQFKDVCATLEEHYRDMQD
MEFTVEHGKLFMLQTRNGKRTAQAALKIACDFVDEGMRTEKEAVAMIDPRNLDTLLHPQFDAAALKAATPAGRGLGASPG
AACGKIVFSAEDAEEWNARGEKVVLVRLETSPEDITGMKASQGILTVRGGMTSHAAVVARGMGTCCVSGCGDIAMDEANK
KFTLAGKEYHEGDYISIDGTTGNIYDGQIPTVDAKIAGEFGRVMEWADKYRKLKVRTNADTPKDAKKARELGAEGIGLCR
TEHMFFEEDRIAAFREMICSDTVEEREAALDKILPYQQGDFEALYEALEGNPVTIRFLDPPLHEFVPTEEEDIKKLAESQ
GKSVETIKNIIASLHEFNPMMGHRGCRLAVTYPEIAKMQTRAVIRAAINVKKVHPDWNVKPEIMIPLICEVKELKYVKKT
VVETADEEIKAAGIDLEYEVGTMIEIPRAALTADEIAKEADFFCFGTNDLTQMTFGFSRDDAGKFLNAYYESKIFENDPF
AKLDQNGVGKLMEMTIKLGRPVNPNLHIGICGEHGGDPSSVAFCHKIGLDYVSCSPFRVPIARLAAAQAAIAEEK

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate. In E.histolytica and C.symbiosus, PPDK functions in the direction of ATP synthesis [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=469, Percent_Identity=26.0127931769723, Blast_Score=130, Evalue=4e-31,
Organism=Escherichia coli, GI1787994, Length=729, Percent_Identity=24.9657064471879, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI48994992, Length=447, Percent_Identity=25.503355704698, Blast_Score=91, Evalue=4e-19,
Organism=Escherichia coli, GI1789193, Length=471, Percent_Identity=23.3545647558386, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 97155; Mature: 97023

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKKWVYLFTEGNADMRELLGGKGANLAEMTNIGLPVPQGFTITTEACTQYYEDGREINN
CCCEEEEEEECCCCHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHH
EIMGQINEHIEKMEQITGKKFGDMENPLLVSVRSGARASMPGMMDTILNLGLNEDVVNVI
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHH
AQKSGNPRWAWDCYRRFIQMYSDVVMEVGKKYFEELIDKMKDERGVKLDVELTADDLKEL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHH
ATQFKAEYKSKIGKDFPDDPKEQLYGAIKAVFRSWDNPRANVYRRDNDIPYSWGTAVNVQ
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEH
SMAFGNMGDDCGTGVAFTRDPATGAKGLFGEFLTNAQGEDVVAGVRTPMHIQEMSEKFPE
HHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHH
AFEQFKDVCATLEEHYRDMQDMEFTVEHGKLFMLQTRNGKRTAQAALKIACDFVDEGMRT
HHHHHHHHHHHHHHHHCCHHHCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCC
EKEAVAMIDPRNLDTLLHPQFDAAALKAATPAGRGLGASPGAACGKIVFSAEDAEEWNAR
CHHHEEEECCCCCCHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHCCC
GEKVVLVRLETSPEDITGMKASQGILTVRGGMTSHAAVVARGMGTCCVSGCGDIAMDEAN
CCEEEEEEEECCCCHHCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHCCCCCHHCCCCC
KKFTLAGKEYHEGDYISIDGTTGNIYDGQIPTVDAKIAGEFGRVMEWADKYRKLKVRTNA
CEEEECCCEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECC
DTPKDAKKARELGAEGIGLCRTEHMFFEEDRIAAFREMICSDTVEEREAALDKILPYQQG
CCCHHHHHHHHCCCCCCCEEEHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCC
DFEALYEALEGNPVTIRFLDPPLHEFVPTEEEDIKKLAESQGKSVETIKNIIASLHEFNP
CHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCH
MMGHRGCRLAVTYPEIAKMQTRAVIRAAINVKKVHPDWNVKPEIMIPLICEVKELKYVKK
HHCCCCCEEEEECHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEHHHHHHHHHHHHHHH
TVVETADEEIKAAGIDLEYEVGTMIEIPRAALTADEIAKEADFFCFGTNDLTQMTFGFSR
HHHHHHHHHHHHCCCCEEECCCCCEECCHHHHHHHHHHHHCCEEEECCCCHHHHHCCCCC
DDAGKFLNAYYESKIFENDPFAKLDQNGVGKLMEMTIKLGRPVNPNLHIGICGEHGGDPS
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCH
SVAFCHKIGLDYVSCSPFRVPIARLAAAQAAIAEEK
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKKWVYLFTEGNADMRELLGGKGANLAEMTNIGLPVPQGFTITTEACTQYYEDGREINN
CCEEEEEEECCCCHHHHHHCCCCCCHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHH
EIMGQINEHIEKMEQITGKKFGDMENPLLVSVRSGARASMPGMMDTILNLGLNEDVVNVI
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCHHHHHHH
AQKSGNPRWAWDCYRRFIQMYSDVVMEVGKKYFEELIDKMKDERGVKLDVELTADDLKEL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHH
ATQFKAEYKSKIGKDFPDDPKEQLYGAIKAVFRSWDNPRANVYRRDNDIPYSWGTAVNVQ
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCEEEEH
SMAFGNMGDDCGTGVAFTRDPATGAKGLFGEFLTNAQGEDVVAGVRTPMHIQEMSEKFPE
HHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHH
AFEQFKDVCATLEEHYRDMQDMEFTVEHGKLFMLQTRNGKRTAQAALKIACDFVDEGMRT
HHHHHHHHHHHHHHHHCCHHHCEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCC
EKEAVAMIDPRNLDTLLHPQFDAAALKAATPAGRGLGASPGAACGKIVFSAEDAEEWNAR
CHHHEEEECCCCCCHHHCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCHHHHCCC
GEKVVLVRLETSPEDITGMKASQGILTVRGGMTSHAAVVARGMGTCCVSGCGDIAMDEAN
CCEEEEEEEECCCCHHCCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHCCCCCHHCCCCC
KKFTLAGKEYHEGDYISIDGTTGNIYDGQIPTVDAKIAGEFGRVMEWADKYRKLKVRTNA
CEEEECCCEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECC
DTPKDAKKARELGAEGIGLCRTEHMFFEEDRIAAFREMICSDTVEEREAALDKILPYQQG
CCCHHHHHHHHCCCCCCCEEEHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCC
DFEALYEALEGNPVTIRFLDPPLHEFVPTEEEDIKKLAESQGKSVETIKNIIASLHEFNP
CHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCH
MMGHRGCRLAVTYPEIAKMQTRAVIRAAINVKKVHPDWNVKPEIMIPLICEVKELKYVKK
HHCCCCCEEEEECHHHHHHHHHHHHHHHHHHEEECCCCCCCCCEEHHHHHHHHHHHHHHH
TVVETADEEIKAAGIDLEYEVGTMIEIPRAALTADEIAKEADFFCFGTNDLTQMTFGFSR
HHHHHHHHHHHHCCCCEEECCCCCEECCHHHHHHHHHHHHCCEEEECCCCHHHHHCCCCC
DDAGKFLNAYYESKIFENDPFAKLDQNGVGKLMEMTIKLGRPVNPNLHIGICGEHGGDPS
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCH
SVAFCHKIGLDYVSCSPFRVPIARLAAAQAAIAEEK
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2176881; 6257292; 11695893; 11468288; 8610096; 9753432 [H]