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The map label for this gene is cysG [H]

Identifier: 238916399

GI number: 238916399

Start: 464274

End: 465650

Strand: Direct

Name: cysG [H]

Synonym: EUBELI_00453

Alternate gene names: 238916399

Gene position: 464274-465650 (Clockwise)

Preceding gene: 238916398

Following gene: 238916400

Centisome position: 21.65

GC content: 42.56

Gene sequence:

>1377_bases
ATGAGTTATTTTCCGTTTTTTAGAGAAATAGAAGGGGAGAAATGCCTTGTTATCGGTGGCGGTAAGGTGGCAGCAAGAAA
GATAAGCAGGCTGAAGGGATTTGGCGTGAAGATTAAAGTTGTTGCACCGTCTATAGTTTCTGACATAGAGGCAGTGGCGC
AGGCTTGCGATAATATAAGTGTTGAAAGAAGAATATTCACACCACAAGATATTGAAAATGTGGATTTTGTTATCGCGGCG
GCTGGTGAAGAAGTGGATAAAGAAGCTACAAGATTGTGCAAAGAAAAGGCAATTCCTGTAAATGTTGTGGATAACCAGGA
GTTATGTGATTTTATATTCCCTTCAATTGTGCAGAAGGGTGATATGGTTGCAGGCTTTGTTACAGGTGGGAAGAGTCCGC
ATGTTGCAGCTAAGTTAAGAAGAGATTTTGAAGCAAATGTATCTGATAATATAGAGAGCATATTAGATTATCTTTCAGAG
GTCAGGACACTTGCCAAAGAAAAGATAGATGATGACAGAAAAAGAGCAGCATTTATAAGAGAAGTTTCTGAATTCTGCAT
GAAAGCAGACAGAGTGTGCAGCAACAAGGAAGAAAATGCATTTTTACAGAAATATCTTGATAACAGGAGTGAGACAGTGC
TTCCGGGGGCAGCACTTGTTGGAGCAGGATGCGGCTCATATGAACTCATCACAATAAAAGGTCTTAATGAGATAAGACGG
GCAGAGGTTATAGTGTATGATGATTTGATTGATGAGCATCTGCTGGAATTTGCACCGGAGAGCTGTGAGCTTATATACGC
CGGAAAGAGGAGTGGCAGACATTCCAAGGCACAGGAGGAGATTAATGAGCTTCTTGTAGAAAAAGCTCTTGAAGGCAGAT
ATGTTGTGAGACTAAAAGGTGGTGACCCTTATGTGTTCGGCAGGGGCGGAGAGGAAGCACTTGCACTTAAAGAACATGGA
ATACCAGTGCATGAAGTTCCGGGGGTTACATCAGGTATCGGTCTGTGTGGAATGGCAGGAATTCCGGTGACACACAGGGG
TGCAAGCCGTGGATTTCATGTGATTACCGGACATACGGCAGATAATCTGTCACCAGAGGTTGAGGAAATCAGGTGGAAAT
CATATGCGAAGCTTGATGAAACATTTGTATTTCTTATGGGTTTAAAGAGTATAGACATGATTACGGAAAAGCTGATGAGA
TATGGAAAGTTGTCAGATACGCCGGTTGCGGTAATTCACGGTGAGAATACAGATGGTACATATGTAAAGCTTGTCGGAAC
ACTTTCGGATATTGCAGCAAAGGTGAAAGAAGTAGACTTCCCATCACCAGCTATTATAGTTGTTGGCGAGGTTGCGTCAC
TGGAATTGACGGATTAG

Upstream 100 bases:

>100_bases
GCTATTATAATAGTCACGAGCAAAATTTATCTACTAAATTGCTGAATATGCGACTATTTATAGGTATCATGACAATAATT
ACAAGAAAAAGGGGAACAGG

Downstream 100 bases:

>100_bases
CAGAAACTTAAGATGCGCTGAAAAGCAGCGCAGGAATAGAGGTTAACATGGATAAAGACAGACTTGACAGACAGATAGAG
TTTATAAGAGAGATTGATAA

Product: uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSYFPFFREIEGEKCLVIGGGKVAARKISRLKGFGVKIKVVAPSIVSDIEAVAQACDNISVERRIFTPQDIENVDFVIAA
AGEEVDKEATRLCKEKAIPVNVVDNQELCDFIFPSIVQKGDMVAGFVTGGKSPHVAAKLRRDFEANVSDNIESILDYLSE
VRTLAKEKIDDDRKRAAFIREVSEFCMKADRVCSNKEENAFLQKYLDNRSETVLPGAALVGAGCGSYELITIKGLNEIRR
AEVIVYDDLIDEHLLEFAPESCELIYAGKRSGRHSKAQEEINELLVEKALEGRYVVRLKGGDPYVFGRGGEEALALKEHG
IPVHEVPGVTSGIGLCGMAGIPVTHRGASRGFHVITGHTADNLSPEVEEIRWKSYAKLDETFVFLMGLKSIDMITEKLMR
YGKLSDTPVAVIHGENTDGTYVKLVGTLSDIAAKVKEVDFPSPAIIVVGEVASLELTD

Sequences:

>Translated_458_residues
MSYFPFFREIEGEKCLVIGGGKVAARKISRLKGFGVKIKVVAPSIVSDIEAVAQACDNISVERRIFTPQDIENVDFVIAA
AGEEVDKEATRLCKEKAIPVNVVDNQELCDFIFPSIVQKGDMVAGFVTGGKSPHVAAKLRRDFEANVSDNIESILDYLSE
VRTLAKEKIDDDRKRAAFIREVSEFCMKADRVCSNKEENAFLQKYLDNRSETVLPGAALVGAGCGSYELITIKGLNEIRR
AEVIVYDDLIDEHLLEFAPESCELIYAGKRSGRHSKAQEEINELLVEKALEGRYVVRLKGGDPYVFGRGGEEALALKEHG
IPVHEVPGVTSGIGLCGMAGIPVTHRGASRGFHVITGHTADNLSPEVEEIRWKSYAKLDETFVFLMGLKSIDMITEKLMR
YGKLSDTPVAVIHGENTDGTYVKLVGTLSDIAAKVKEVDFPSPAIIVVGEVASLELTD
>Mature_457_residues
SYFPFFREIEGEKCLVIGGGKVAARKISRLKGFGVKIKVVAPSIVSDIEAVAQACDNISVERRIFTPQDIENVDFVIAAA
GEEVDKEATRLCKEKAIPVNVVDNQELCDFIFPSIVQKGDMVAGFVTGGKSPHVAAKLRRDFEANVSDNIESILDYLSEV
RTLAKEKIDDDRKRAAFIREVSEFCMKADRVCSNKEENAFLQKYLDNRSETVLPGAALVGAGCGSYELITIKGLNEIRRA
EVIVYDDLIDEHLLEFAPESCELIYAGKRSGRHSKAQEEINELLVEKALEGRYVVRLKGGDPYVFGRGGEEALALKEHGI
PVHEVPGVTSGIGLCGMAGIPVTHRGASRGFHVITGHTADNLSPEVEEIRWKSYAKLDETFVFLMGLKSIDMITEKLMRY
GKLSDTPVAVIHGENTDGTYVKLVGTLSDIAAKVKEVDFPSPAIIVVGEVASLELTD

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=462, Percent_Identity=31.8181818181818, Blast_Score=243, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6322922, Length=241, Percent_Identity=34.4398340248963, Blast_Score=121, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 50183; Mature: 50052

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYFPFFREIEGEKCLVIGGGKVAARKISRLKGFGVKIKVVAPSIVSDIEAVAQACDNIS
CCCCCCHHHCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCC
VERRIFTPQDIENVDFVIAAAGEEVDKEATRLCKEKAIPVNVVDNQELCDFIFPSIVQKG
EEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCC
DMVAGFVTGGKSPHVAAKLRRDFEANVSDNIESILDYLSEVRTLAKEKIDDDRKRAAFIR
CEEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EVSEFCMKADRVCSNKEENAFLQKYLDNRSETVLPGAALVGAGCGSYELITIKGLNEIRR
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEECCCHHHHCCCCCCEEEEEECCCHHHHH
AEVIVYDDLIDEHLLEFAPESCELIYAGKRSGRHSKAQEEINELLVEKALEGRYVVRLKG
HEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEC
GDPYVFGRGGEEALALKEHGIPVHEVPGVTSGIGLCGMAGIPVTHRGASRGFHVITGHTA
CCCEEECCCCCCEEEHHHCCCCHHHCCCCCCCCCHHCCCCCCEECCCCCCCEEEEECCCC
DNLSPEVEEIRWKSYAKLDETFVFLMGLKSIDMITEKLMRYGKLSDTPVAVIHGENTDGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
YVKLVGTLSDIAAKVKEVDFPSPAIIVVGEVASLELTD
EEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECC
>Mature Secondary Structure 
SYFPFFREIEGEKCLVIGGGKVAARKISRLKGFGVKIKVVAPSIVSDIEAVAQACDNIS
CCCCCHHHCCCCEEEEEECCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCC
VERRIFTPQDIENVDFVIAAAGEEVDKEATRLCKEKAIPVNVVDNQELCDFIFPSIVQKG
EEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHCCC
DMVAGFVTGGKSPHVAAKLRRDFEANVSDNIESILDYLSEVRTLAKEKIDDDRKRAAFIR
CEEEEEEECCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
EVSEFCMKADRVCSNKEENAFLQKYLDNRSETVLPGAALVGAGCGSYELITIKGLNEIRR
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEECCCHHHHCCCCCCEEEEEECCCHHHHH
AEVIVYDDLIDEHLLEFAPESCELIYAGKRSGRHSKAQEEINELLVEKALEGRYVVRLKG
HEEEEEHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEC
GDPYVFGRGGEEALALKEHGIPVHEVPGVTSGIGLCGMAGIPVTHRGASRGFHVITGHTA
CCCEEECCCCCCEEEHHHCCCCHHHCCCCCCCCCHHCCCCCCEECCCCCCCEEEEECCCC
DNLSPEVEEIRWKSYAKLDETFVFLMGLKSIDMITEKLMRYGKLSDTPVAVIHGENTDGT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCC
YVKLVGTLSDIAAKVKEVDFPSPAIIVVGEVASLELTD
EEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA