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The map label for this gene is purF [H]

Identifier: 238916398

GI number: 238916398

Start: 462628

End: 464070

Strand: Direct

Name: purF [H]

Synonym: EUBELI_00452

Alternate gene names: 238916398

Gene position: 462628-464070 (Clockwise)

Preceding gene: 238916397

Following gene: 238916399

Centisome position: 21.58

GC content: 40.4

Gene sequence:

>1443_bases
ATGAATCAGATTGATATTAATGACAGCCACTATGCTGAAGATGAACTTCATGAGGAATGCGGAGTCTTTGGTGTATATGA
TTTCGATGGCAATGATGTTTCATCTACAATATATTATGGCCTTTTTGCCCTTCAGCACAGAGGACAGGAAAGCTGCGGTA
TAGCAGTCAGTGATACAGAAGGACCTAAGGGAAAGGTTCTTTCATATAAGGATATGGGACTTGTTAATGAAGTTTTTAAT
CCTGAAAAGCTTGAAAAGCTTAATGGTAATATAGGTGTCGGACATGTAAGATATTCAACAGCCGGAAGCAGCAGCCGTGA
GAATGCACAGCCACTTGTTCTTAACTATGTTAAGGGAACTCTTGGTATGGCACATAACGGTAATCTTCTTAATGCGGTTG
AACTGCGTGAGGAGCTTTCTTATACAGGAGCTATCTTCCAGACAACTATTGATTCGGAGGTTATTGCTTACTTAATCGCA
AGAGAAAGACTTAATGTTCCTACTGTTGAGGGAGCTGTTTTAAATGCGATGAAGAAGATTAAGGGTGCTTATTCACTTAT
CGTAATGAGTCCTAGAAAGCTTATCGGAGCAAGAGACCCATTTGGTTTCAAGCCATTATGTATCGGTAAGAGAGATAATG
CATATTTCTTATCATCAGAGACATGTGCACTCGATACTGTAGGTGCAGAGTTTGTAAGAGATGTTGAGCCGGGTGAAGTT
GTTACTATCACTAAAGACGGAATCAAGTCTGATAAGTCACTCTGCCAGAAAAATACAGCAAGATGCATATTTGAATACAT
TTATTTTGCAAGACCAGACAGCAAGATTGACGGAATGGGCGTATATGAGTCAAGAATTAATGCAGGTAGAATCTTAGCAA
AGACTCATCCTGTAGAAGCTGATATTGTTGTAGGTGTTCCAGAATCAGGTAATCCTGCAGCACTTGGTTTCTCAATGGAA
TCAGGAATTCCTTATGGTAATGCATTTATCAAGAATAACTACGTTGGACGAACATTCATCAAGCCTAAGCAGGAACAGAG
AGAGTCAAGTGTTAAGGTTAAGCTGAATGTACTTAAGGAAGCAGTTGCAGGCAAGAGAGTTGTCATGATTGATGACTCAA
TTGTACGAGGAACAACAAGTGCGAGAATTGTAAGTCTTCTTAAGGCAGCAGGTGCTAAGGAAGTTCATGTAAGAATAAGT
TCACCAGCGTTCCTTCACCCATGTTATTTTGGAACAGATATTCCTTCAGAAGACCAGCTTATCGCATCAGGCCATTCTGT
AGATGAAATCTGTGAAATTATCGGTGCAGATTCATTAGGCTATCTTGAGGTTGACAAGTTAAGTGAGATGATATGTGGAC
AGACAGGTTACTGTGATGCTTGCTTTACAGGCAATTATCCAATTGAGCCGCCTAAGATTGATATCCGCGGCGAGATGGGC
TGA

Upstream 100 bases:

>100_bases
TAATCGAGAAGACAGTTGAAAAGTACAAGGAAGCATATAAGCTTCTTACAGGCAAGGATTTTTCCCGCAAATAATCACAG
TCGATAAGGAATGGAGAATT

Downstream 100 bases:

>100_bases
TTTGGTAGGCATTTTTAAGCAGAATAATAGAATATATGACACAAGATGCCTACTAAAAGCATGGAATATGTTATTTAGTA
GGCATTTTTAGTAAAAATAT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MNQIDINDSHYAEDELHEECGVFGVYDFDGNDVSSTIYYGLFALQHRGQESCGIAVSDTEGPKGKVLSYKDMGLVNEVFN
PEKLEKLNGNIGVGHVRYSTAGSSSRENAQPLVLNYVKGTLGMAHNGNLLNAVELREELSYTGAIFQTTIDSEVIAYLIA
RERLNVPTVEGAVLNAMKKIKGAYSLIVMSPRKLIGARDPFGFKPLCIGKRDNAYFLSSETCALDTVGAEFVRDVEPGEV
VTITKDGIKSDKSLCQKNTARCIFEYIYFARPDSKIDGMGVYESRINAGRILAKTHPVEADIVVGVPESGNPAALGFSME
SGIPYGNAFIKNNYVGRTFIKPKQEQRESSVKVKLNVLKEAVAGKRVVMIDDSIVRGTTSARIVSLLKAAGAKEVHVRIS
SPAFLHPCYFGTDIPSEDQLIASGHSVDEICEIIGADSLGYLEVDKLSEMICGQTGYCDACFTGNYPIEPPKIDIRGEMG

Sequences:

>Translated_480_residues
MNQIDINDSHYAEDELHEECGVFGVYDFDGNDVSSTIYYGLFALQHRGQESCGIAVSDTEGPKGKVLSYKDMGLVNEVFN
PEKLEKLNGNIGVGHVRYSTAGSSSRENAQPLVLNYVKGTLGMAHNGNLLNAVELREELSYTGAIFQTTIDSEVIAYLIA
RERLNVPTVEGAVLNAMKKIKGAYSLIVMSPRKLIGARDPFGFKPLCIGKRDNAYFLSSETCALDTVGAEFVRDVEPGEV
VTITKDGIKSDKSLCQKNTARCIFEYIYFARPDSKIDGMGVYESRINAGRILAKTHPVEADIVVGVPESGNPAALGFSME
SGIPYGNAFIKNNYVGRTFIKPKQEQRESSVKVKLNVLKEAVAGKRVVMIDDSIVRGTTSARIVSLLKAAGAKEVHVRIS
SPAFLHPCYFGTDIPSEDQLIASGHSVDEICEIIGADSLGYLEVDKLSEMICGQTGYCDACFTGNYPIEPPKIDIRGEMG
>Mature_480_residues
MNQIDINDSHYAEDELHEECGVFGVYDFDGNDVSSTIYYGLFALQHRGQESCGIAVSDTEGPKGKVLSYKDMGLVNEVFN
PEKLEKLNGNIGVGHVRYSTAGSSSRENAQPLVLNYVKGTLGMAHNGNLLNAVELREELSYTGAIFQTTIDSEVIAYLIA
RERLNVPTVEGAVLNAMKKIKGAYSLIVMSPRKLIGARDPFGFKPLCIGKRDNAYFLSSETCALDTVGAEFVRDVEPGEV
VTITKDGIKSDKSLCQKNTARCIFEYIYFARPDSKIDGMGVYESRINAGRILAKTHPVEADIVVGVPESGNPAALGFSME
SGIPYGNAFIKNNYVGRTFIKPKQEQRESSVKVKLNVLKEAVAGKRVVMIDDSIVRGTTSARIVSLLKAAGAKEVHVRIS
SPAFLHPCYFGTDIPSEDQLIASGHSVDEICEIIGADSLGYLEVDKLSEMICGQTGYCDACFTGNYPIEPPKIDIRGEMG

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=509, Percent_Identity=40.0785854616896, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1788651, Length=453, Percent_Identity=40.3973509933775, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1790167, Length=232, Percent_Identity=32.7586206896552, Blast_Score=110, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=41.25, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17539970, Length=221, Percent_Identity=28.0542986425339, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17532897, Length=215, Percent_Identity=27.4418604651163, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17532899, Length=215, Percent_Identity=27.4418604651163, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323958, Length=475, Percent_Identity=36.2105263157895, Blast_Score=273, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24659598, Length=483, Percent_Identity=41.8219461697723, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI28573187, Length=489, Percent_Identity=42.5357873210634, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24659604, Length=461, Percent_Identity=42.2993492407809, Blast_Score=369, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52247; Mature: 52247

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00165 DEHYDRATASE_SER_THR ; PS00283 SOYBEAN_KUNITZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQIDINDSHYAEDELHEECGVFGVYDFDGNDVSSTIYYGLFALQHRGQESCGIAVSDTE
CCEECCCCCCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCC
GPKGKVLSYKDMGLVNEVFNPEKLEKLNGNIGVGHVRYSTAGSSSRENAQPLVLNYVKGT
CCCCCEEEECCCCHHHHHCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCEEEEEHHHH
LGMAHNGNLLNAVELREELSYTGAIFQTTIDSEVIAYLIARERLNVPTVEGAVLNAMKKI
HCCCCCCCEEHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
KGAYSLIVMSPRKLIGARDPFGFKPLCIGKRDNAYFLSSETCALDTVGAEFVRDVEPGEV
CCCEEEEEECCHHHHCCCCCCCCCCEEECCCCCEEEEECCCCEEHHHHHHHHHCCCCCCE
VTITKDGIKSDKSLCQKNTARCIFEYIYFARPDSKIDGMGVYESRINAGRILAKTHPVEA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEECCCCCE
DIVVGVPESGNPAALGFSMESGIPYGNAFIKNNYVGRTFIKPKQEQRESSVKVKLNVLKE
EEEEECCCCCCCEEEEEEHHCCCCCCCEEEECCCCCCEECCCCHHHHHCCEEEEHHHHHH
AVAGKRVVMIDDSIVRGTTSARIVSLLKAAGAKEVHVRISSPAFLHPCYFGTDIPSEDQL
HHCCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCEECCEECCCCCCCCCCE
IASGHSVDEICEIIGADSLGYLEVDKLSEMICGQTGYCDACFTGNYPIEPPKIDIRGEMG
EECCCCHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MNQIDINDSHYAEDELHEECGVFGVYDFDGNDVSSTIYYGLFALQHRGQESCGIAVSDTE
CCEECCCCCCCCHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEECCC
GPKGKVLSYKDMGLVNEVFNPEKLEKLNGNIGVGHVRYSTAGSSSRENAQPLVLNYVKGT
CCCCCEEEECCCCHHHHHCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCEEEEEHHHH
LGMAHNGNLLNAVELREELSYTGAIFQTTIDSEVIAYLIARERLNVPTVEGAVLNAMKKI
HCCCCCCCEEHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
KGAYSLIVMSPRKLIGARDPFGFKPLCIGKRDNAYFLSSETCALDTVGAEFVRDVEPGEV
CCCEEEEEECCHHHHCCCCCCCCCCEEECCCCCEEEEECCCCEEHHHHHHHHHCCCCCCE
VTITKDGIKSDKSLCQKNTARCIFEYIYFARPDSKIDGMGVYESRINAGRILAKTHPVEA
EEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEECCCCCE
DIVVGVPESGNPAALGFSMESGIPYGNAFIKNNYVGRTFIKPKQEQRESSVKVKLNVLKE
EEEEECCCCCCCEEEEEEHHCCCCCCCEEEECCCCCCEECCCCHHHHHCCEEEEHHHHHH
AVAGKRVVMIDDSIVRGTTSARIVSLLKAAGAKEVHVRISSPAFLHPCYFGTDIPSEDQL
HHCCCEEEEECCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCEECCEECCCCCCCCCCE
IASGHSVDEICEIIGADSLGYLEVDKLSEMICGQTGYCDACFTGNYPIEPPKIDIRGEMG
EECCCCHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]