Click here to switch to the map view.

The map label for this gene is mrkB [H]

Identifier: 238896389

GI number: 238896389

Start: 4372371

End: 4373072

Strand: Reverse

Name: mrkB [H]

Synonym: KP1_4560

Alternate gene names: 238896389

Gene position: 4373072-4372371 (Counterclockwise)

Preceding gene: 238896390

Following gene: 238896388

Centisome position: 83.32

GC content: 54.7

Gene sequence:

>702_bases
ATGAAGCGTATTGCCCTTTTTTTCTGTTTCATTTTTAGTTTTGCGGCCCATGCCAACAATATTATTGTTAACGGTACCCG
CTTTATTTATCCAGGAAATGAAAAAGAAATAACGGTACAGCTTTCAAATAACGCCGACCGTCCGGCGCTGGCGCAGGCCT
GGCTGGATAACGGCGATGCCGATGCCACACCGGATACCATTACCACCCCGTTTATTATCACCCCGCCGATTTCGCGCGTT
GACGCCAAGAGCGGCCAGACGCTGCGTATTAAGCTCGGTAGCAGCGCCGGTCTGGCGAAAGATAAAGAGACGCTGTGGTG
GCTGAATCTGCTGGAAATTCCGCCGGTGGTGGCCAATCAGAAAAATGAAGGCCAGAACGTGCTGCAGCTGGCGATCCGTT
CGCGCTTTAAATTTATTTATCGTCCTGCCGGACTGGGCAACCGCGATGCGGCGGCCGAGAAGCTGACCCTGACCGCCAGC
GGCAGTAGTCTCGCCATCAACAACCCGACGCCGTTCTACATTACCGTCAGCCGTATTTCCCGCGACGGCGGTAAAGCCCT
GAACAGCAAAACCGTGATGCTGGCGCCGCAGTCCAGCCAGACGGTGGCGCTCTCCTCCGCCGTCAACCGCGGCGAAACCC
TGACCGTTAACAACATTAATGACTATGGCGCCGATGTCGCAGTGAAAGTGGCCGTTAAATAA

Upstream 100 bases:

>100_bases
AGTAATTCCGTCAGAAAATAAAACTGCCCCGCGATGGGGCAGTTAATAACAATAATAAAGCGGCAATGTCCGCCGCTTGT
ATTTTTATTCTGAGGTCAGC

Downstream 100 bases:

>100_bases
GGGAAAAGCTAATGAAGCAGAGGTCATTCTGCCCGGGAAGATTAAGCACCGCGATCGCCATCGCGCTGTGCTGCTTTCCG
CCATTTTCCAGCGGACAGGA

Product: fimbrial chaperone protein mrkB precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKRIALFFCFIFSFAAHANNIIVNGTRFIYPGNEKEITVQLSNNADRPALAQAWLDNGDADATPDTITTPFIITPPISRV
DAKSGQTLRIKLGSSAGLAKDKETLWWLNLLEIPPVVANQKNEGQNVLQLAIRSRFKFIYRPAGLGNRDAAAEKLTLTAS
GSSLAINNPTPFYITVSRISRDGGKALNSKTVMLAPQSSQTVALSSAVNRGETLTVNNINDYGADVAVKVAVK

Sequences:

>Translated_233_residues
MKRIALFFCFIFSFAAHANNIIVNGTRFIYPGNEKEITVQLSNNADRPALAQAWLDNGDADATPDTITTPFIITPPISRV
DAKSGQTLRIKLGSSAGLAKDKETLWWLNLLEIPPVVANQKNEGQNVLQLAIRSRFKFIYRPAGLGNRDAAAEKLTLTAS
GSSLAINNPTPFYITVSRISRDGGKALNSKTVMLAPQSSQTVALSSAVNRGETLTVNNINDYGADVAVKVAVK
>Mature_233_residues
MKRIALFFCFIFSFAAHANNIIVNGTRFIYPGNEKEITVQLSNNADRPALAQAWLDNGDADATPDTITTPFIITPPISRV
DAKSGQTLRIKLGSSAGLAKDKETLWWLNLLEIPPVVANQKNEGQNVLQLAIRSRFKFIYRPAGLGNRDAAAEKLTLTAS
GSSLAINNPTPFYITVSRISRDGGKALNSKTVMLAPQSSQTVALSSAVNRGETLTVNNINDYGADVAVKVAVK

Specific function: Mediates assembly of pili by forming soluble multimeric complexes with pili subunits as an intermediate step in the assembly process. This protein is involved in type 3 pili assembly [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1786333, Length=232, Percent_Identity=40.0862068965517, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1788428, Length=231, Percent_Identity=38.0952380952381, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1790771, Length=223, Percent_Identity=37.219730941704, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1786743, Length=229, Percent_Identity=35.8078602620087, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786936, Length=206, Percent_Identity=34.4660194174757, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1788677, Length=210, Percent_Identity=31.4285714285714, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789532, Length=230, Percent_Identity=33.0434782608696, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787171, Length=215, Percent_Identity=32.5581395348837, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082203, Length=211, Percent_Identity=29.3838862559242, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI87081806, Length=238, Percent_Identity=30.2521008403361, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 25161; Mature: 25161

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS00635 PILI_CHAPERONE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIALFFCFIFSFAAHANNIIVNGTRFIYPGNEKEITVQLSNNADRPALAQAWLDNGDA
CCHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCEEEEEECCCCCCCHHHHHHHCCCCC
DATPDTITTPFIITPPISRVDAKSGQTLRIKLGSSAGLAKDKETLWWLNLLEIPPVVANQ
CCCCCCCCCCEEECCCCHHCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCC
KNEGQNVLQLAIRSRFKFIYRPAGLGNRDAAAEKLTLTASGSSLAINNPTPFYITVSRIS
CCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEEEEEEC
RDGGKALNSKTVMLAPQSSQTVALSSAVNRGETLTVNNINDYGADVAVKVAVK
CCCCCCCCCCEEEEECCCCCEEEEEHHCCCCCEEEEECCCCCCCCEEEEEEEC
>Mature Secondary Structure
MKRIALFFCFIFSFAAHANNIIVNGTRFIYPGNEKEITVQLSNNADRPALAQAWLDNGDA
CCHHHHHHHHHHHHHHCCCCEEECCEEEEECCCCCEEEEEECCCCCCCHHHHHHHCCCCC
DATPDTITTPFIITPPISRVDAKSGQTLRIKLGSSAGLAKDKETLWWLNLLEIPPVVANQ
CCCCCCCCCCEEECCCCHHCCCCCCCEEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCC
KNEGQNVLQLAIRSRFKFIYRPAGLGNRDAAAEKLTLTASGSSLAINNPTPFYITVSRIS
CCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCEEEEEEECCCEEEECCCCCEEEEEEEEC
RDGGKALNSKTVMLAPQSSQTVALSSAVNRGETLTVNNINDYGADVAVKVAVK
CCCCCCCCCCEEEEECCCCCEEEEEHHCCCCCEEEEECCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1670938 [H]