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The map label for this gene is amiC [H]

Identifier: 238896331

GI number: 238896331

Start: 4305669

End: 4306958

Strand: Reverse

Name: amiC [H]

Synonym: KP1_4494

Alternate gene names: 238896331

Gene position: 4306958-4305669 (Counterclockwise)

Preceding gene: 238896334

Following gene: 238896330

Centisome position: 82.06

GC content: 59.92

Gene sequence:

>1290_bases
TTGGGTATAAATGCGTTTTTATTGCTTGGGAGTGGCATGTCGGGAAACAACTCTGGATTAAGCCGTCGCCGTTTATTGCA
GGGGGCGGGCGCCATGTGGTTAATGAGCGTGAGCCCTGTTGGGCTGGCCGCCGCCGCGCGGGTGGTCGCTGTTCGCGTCT
GGCCAGCATCGACCTACACCCGCGTCACGGTGGAATCGAATCATGTGCTGAAATACCGGCAGTTTGCCCTCAGTAATCCG
GAACGGGTAGTGGTCGATCTGGAGGGGGTTAACCTGAACTCGGTACTGAAGGGCATGGGGGGGCAGATCCGCGCTGACGA
TCCCTTTATTAAGTCCGCCCGGGTGGGCCAGTTTGATCCGCAAACCGTGCGCATGGTCTTCGAACTCAAGCAGAACGTTA
AGCCGCAGCTGTTTGCCCTCGCGCCGGTGGCCGGCTTTAAAGAGCGTCTGGTGATGGATCTCTACCCCGCCAACGCTACC
GATGTTCAGGATCCGCTGCTGGCGCTGCTCGAGGATTACAACAAAGGCGATCTGGAGCGGCAGGTGCCGCCGGCGCAAAG
CGGGCCGCAGCCGGGCAAAGCCGGGCGCGACCGGCCGATTGTCATCATGCTCGATCCCGGGCACGGCGGCGAAGACTCCG
GCGCCGTGGGGAAGTATCGCACCCGCGAGAAAGATGTGGTGCTACAGATCGCCCGTCGCCTGCGGGCGCTGATCGACAAA
GAAGGCAATATGAAGGCCTATATGACCCGCAACGAAGACGTGTTTATCCCGTTGAAAGTGCGTGTCGCCAAGGCGCAGAA
GCAGCGCGCCGACCTGTTCGTGTCGATTCACGCCGACGCCTTTACCAGCCGCCAGCCCAGCGGCTCTTCGGTGTTTGCCC
TCTCCACCAAGGGGGCCACCAGTACCGCGGCAAAATATCTCGCCCAGACGCAGAACGCCTCGGATCTGATCGGTGGGGTC
AGCAAGAGCGGCGACCGCTATGTCGACCACACCATGTTCGATATGGTGCAGTCCCTCACCATCGCCGACAGCCTCAAATT
CGGAAAGGCGGTGCTGGAGAAAATGGGCAATATCAATAATTTGCACAAGAATCGGGTAGAGCAGGCGGGCTTTGCGGTAC
TGAAGGCGCCTGATATCCCCTCCATTCTGGTGGAGACGGCGTTTATCAGTAACGTGGAAGAAGAGCGGAAACTGAAAACG
GCCAAATTCCAGCAGGAGGTGGCCGAGTCGATCCTCGCCGGGATTAAAGCCTACTTCGCCGACGGCGCGACGCTGGCCCG
CCGCGGATAA

Upstream 100 bases:

>100_bases
AAAAACATGTGACCCGGCGCAGGGTGTCAATGAATGTTTACCCTTTTATAGATGACAGGTTATATGCCTTTCGTTAAAGT
TTCTGGTCAATCTGTTTGTT

Downstream 100 bases:

>100_bases
CCACACAGGAAAGGGACATGGCCTTACTGAAAAGCGCCCATGGCGGCAATATCCGCGAGGCCGCCGCCCTGCTGGGCATA
GCCCCCGGCGAGCTGCTGGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MGINAFLLLGSGMSGNNSGLSRRRLLQGAGAMWLMSVSPVGLAAAARVVAVRVWPASTYTRVTVESNHVLKYRQFALSNP
ERVVVDLEGVNLNSVLKGMGGQIRADDPFIKSARVGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAT
DVQDPLLALLEDYNKGDLERQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYRTREKDVVLQIARRLRALIDK
EGNMKAYMTRNEDVFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGV
SKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLEKMGNINNLHKNRVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKT
AKFQQEVAESILAGIKAYFADGATLARRG

Sequences:

>Translated_429_residues
MGINAFLLLGSGMSGNNSGLSRRRLLQGAGAMWLMSVSPVGLAAAARVVAVRVWPASTYTRVTVESNHVLKYRQFALSNP
ERVVVDLEGVNLNSVLKGMGGQIRADDPFIKSARVGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAT
DVQDPLLALLEDYNKGDLERQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYRTREKDVVLQIARRLRALIDK
EGNMKAYMTRNEDVFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGV
SKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLEKMGNINNLHKNRVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKT
AKFQQEVAESILAGIKAYFADGATLARRG
>Mature_428_residues
GINAFLLLGSGMSGNNSGLSRRRLLQGAGAMWLMSVSPVGLAAAARVVAVRVWPASTYTRVTVESNHVLKYRQFALSNPE
RVVVDLEGVNLNSVLKGMGGQIRADDPFIKSARVGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANATD
VQDPLLALLEDYNKGDLERQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYRTREKDVVLQIARRLRALIDKE
GNMKAYMTRNEDVFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGATSTAAKYLAQTQNASDLIGGVS
KSGDRYVDHTMFDMVQSLTIADSLKFGKAVLEKMGNINNLHKNRVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTA
KFQQEVAESILAGIKAYFADGATLARRG

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=417, Percent_Identity=92.0863309352518, Blast_Score=793, Evalue=0.0,
Organism=Escherichia coli, GI1788776, Length=235, Percent_Identity=47.2340425531915, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1790611, Length=225, Percent_Identity=40.4444444444444, Blast_Score=175, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46642; Mature: 46511

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGINAFLLLGSGMSGNNSGLSRRRLLQGAGAMWLMSVSPVGLAAAARVVAVRVWPASTYT
CCCEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHEEEEEEEEECCCCEE
RVTVESNHVLKYRQFALSNPERVVVDLEGVNLNSVLKGMGGQIRADDPFIKSARVGQFDP
EEEECCCCEEHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCEECCCCCCCCCCCCCCCH
QTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANATDVQDPLLALLEDYNKGDLER
HHHHHHHHHHHCCCCEEEEECCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCCCCC
QVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYRTREKDVVLQIARRLRALIDK
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
EGNMKAYMTRNEDVFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGAT
CCCEEEEEECCCCEEEEEEEEEHHHHHCCCCEEEEEECCHHCCCCCCCCEEEEEECCCCH
STAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLEKMGNINN
HHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LHKNRVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTAKFQQEVAESILAGIKAYFA
HHHHHHHHHCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGATLARRG
CCCHHCCCC
>Mature Secondary Structure 
GINAFLLLGSGMSGNNSGLSRRRLLQGAGAMWLMSVSPVGLAAAARVVAVRVWPASTYT
CCEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEECCCCHHHHHEEEEEEEEECCCCEE
RVTVESNHVLKYRQFALSNPERVVVDLEGVNLNSVLKGMGGQIRADDPFIKSARVGQFDP
EEEECCCCEEHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCEECCCCCCCCCCCCCCCH
QTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANATDVQDPLLALLEDYNKGDLER
HHHHHHHHHHHCCCCEEEEECCCCCHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCCCCC
QVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYRTREKDVVLQIARRLRALIDK
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
EGNMKAYMTRNEDVFIPLKVRVAKAQKQRADLFVSIHADAFTSRQPSGSSVFALSTKGAT
CCCEEEEEECCCCEEEEEEEEEHHHHHCCCCEEEEEECCHHCCCCCCCCEEEEEECCCCH
STAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLKFGKAVLEKMGNINN
HHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
LHKNRVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTAKFQQEVAESILAGIKAYFA
HHHHHHHHHCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGATLARRG
CCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]