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The map label for this gene is rumA [H]

Identifier: 238896246

GI number: 238896246

Start: 4221049

End: 4222353

Strand: Reverse

Name: rumA [H]

Synonym: KP1_4399

Alternate gene names: 238896246

Gene position: 4222353-4221049 (Counterclockwise)

Preceding gene: 238896248

Following gene: 238896245

Centisome position: 80.45

GC content: 60.61

Gene sequence:

>1305_bases
ATGGCGCAATTCTACTCTGCAAAACGGCGTGTGACGACGCGCCAGATCATAACCGTTACAGTCAACGACCTCGATCCCTT
TGGTCAGGGTGTTGCCCGCCACCAGGGGAAGGCGCTGTTCGTGAGCGGCGTGCTGCCCCACGAGCAGGCCGAGGTGGTGC
TGGTGGAAGATAAAAAACAGTACGCCCGCGCCGAGGTGAAACGCCGACTGACCGACAGCCCGCAGCGCCAGGCGCCGCGC
TGCCCCCATTTTGGCGTCTGCGGCGGATGCCAACAACAACATGCCAGCGTTCCGCTTCAGCAGCAAAGTAAACGCGCCGC
GCTGGGGCGACTGATGAAGCGCGAGGTAGATGACGTCATCGCCGGCGCGCCGTGGGGCTACCGCCGTCGCGCACGCTTGA
GCTTGAATTATCAGCCGAAGACCCAACAATTGCAGATGGGTTTTCGCCAGGCCAACGCCAAAGCGATCGTCGATGTGGTG
CAGTGCCCTGTTTTGGTGCCCCAACTTGAGGCATTGCTGCCCGCTGTGCGCGAGTGCTTAAGCGCGCTGAGCGCATTACG
CCATCTCGGCCACGTCGAGCTGGTGCAGGCGGACAATGGCCCGCTGATGGTGCTGCGCCATACCGCAGCCCTGCCGGCGA
CAGACAGGGAAAAACTGGAACGCTTTTCGCAAACTCACGGTCTTTCCCTGTACCTCGCGCCACAAAGCGAGATACTGGAG
CATATTCATGGCGAAGCGCCATGGTATACCTCAGACGGACTACGCTTAGTGTTCAGCCCGCGGGATTTTATCCAGGTCAA
CGACGGCGTAAATCAGCAGATGGTGCGTACCGCACTGGAGTGGCTGGATCTGCGGCCAGAGGACCGGGTGCTGGATCTGT
TCTGCGGGATGGGCAACTTTACCCTGCCGCTGGCGACACGCGCCGCCCATGTGGTGGGCGTGGAAGGGGTGCCGGCGCTG
GTGGAGAAGGGGCGGGAAAACGCCGCCCGCAATGGTTTATCAAATGTCACTTTCTTCCATGAAAATCTGGAAGAAGACGT
CACCCGTCAGGCCTGGGCGAAGCACGGCTTTGATAAAGTGCTGCTGGACCCGGCCCGCGCCGGCGCGCCGGGCGTGATGC
CGCATATAATAAAACTGGCACCGCGGCGGGTGGTGTATGTTTCCTGCAACCCGGCGACGCTGGCGCGAGACAGCGAAACC
CTGTTGCAGGCTGGATATCAGATTCAACGCCTGGCGATGCTGGATATGTTCCCGCATACCGGCCATCTGGAATCAATGGT
GTTGTTTGAGCGCAGGCTTACATAA

Upstream 100 bases:

>100_bases
GGGAGAATATAGACATCATCGTTCTGTGCAGCGTCGCTGCGCCTCGCGGCGCTGCCGCTCTGAACGAGCTCGTTTTATTT
GATTGAGACAACTCTCTACG

Downstream 100 bases:

>100_bases
GGTCATTCACAACGTCGCAGAAGAGCCACCCTGGCCATGAATGCTGACGCGACCTGCAGGATGACATGTAGAAAGTTGAG
GCTTGCCGACTTCGGCAGGC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MAQFYSAKRRVTTRQIITVTVNDLDPFGQGVARHQGKALFVSGVLPHEQAEVVLVEDKKQYARAEVKRRLTDSPQRQAPR
CPHFGVCGGCQQQHASVPLQQQSKRAALGRLMKREVDDVIAGAPWGYRRRARLSLNYQPKTQQLQMGFRQANAKAIVDVV
QCPVLVPQLEALLPAVRECLSALSALRHLGHVELVQADNGPLMVLRHTAALPATDREKLERFSQTHGLSLYLAPQSEILE
HIHGEAPWYTSDGLRLVFSPRDFIQVNDGVNQQMVRTALEWLDLRPEDRVLDLFCGMGNFTLPLATRAAHVVGVEGVPAL
VEKGRENAARNGLSNVTFFHENLEEDVTRQAWAKHGFDKVLLDPARAGAPGVMPHIIKLAPRRVVYVSCNPATLARDSET
LLQAGYQIQRLAMLDMFPHTGHLESMVLFERRLT

Sequences:

>Translated_434_residues
MAQFYSAKRRVTTRQIITVTVNDLDPFGQGVARHQGKALFVSGVLPHEQAEVVLVEDKKQYARAEVKRRLTDSPQRQAPR
CPHFGVCGGCQQQHASVPLQQQSKRAALGRLMKREVDDVIAGAPWGYRRRARLSLNYQPKTQQLQMGFRQANAKAIVDVV
QCPVLVPQLEALLPAVRECLSALSALRHLGHVELVQADNGPLMVLRHTAALPATDREKLERFSQTHGLSLYLAPQSEILE
HIHGEAPWYTSDGLRLVFSPRDFIQVNDGVNQQMVRTALEWLDLRPEDRVLDLFCGMGNFTLPLATRAAHVVGVEGVPAL
VEKGRENAARNGLSNVTFFHENLEEDVTRQAWAKHGFDKVLLDPARAGAPGVMPHIIKLAPRRVVYVSCNPATLARDSET
LLQAGYQIQRLAMLDMFPHTGHLESMVLFERRLT
>Mature_433_residues
AQFYSAKRRVTTRQIITVTVNDLDPFGQGVARHQGKALFVSGVLPHEQAEVVLVEDKKQYARAEVKRRLTDSPQRQAPRC
PHFGVCGGCQQQHASVPLQQQSKRAALGRLMKREVDDVIAGAPWGYRRRARLSLNYQPKTQQLQMGFRQANAKAIVDVVQ
CPVLVPQLEALLPAVRECLSALSALRHLGHVELVQADNGPLMVLRHTAALPATDREKLERFSQTHGLSLYLAPQSEILEH
IHGEAPWYTSDGLRLVFSPRDFIQVNDGVNQQMVRTALEWLDLRPEDRVLDLFCGMGNFTLPLATRAAHVVGVEGVPALV
EKGRENAARNGLSNVTFFHENLEEDVTRQAWAKHGFDKVLLDPARAGAPGVMPHIIKLAPRRVVYVSCNPATLARDSETL
LQAGYQIQRLAMLDMFPHTGHLESMVLFERRLT

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=206, Percent_Identity=33.9805825242718, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI51173878, Length=206, Percent_Identity=33.9805825242718, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI269784680, Length=193, Percent_Identity=29.5336787564767, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI269784676, Length=193, Percent_Identity=29.5336787564767, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI20149688, Length=193, Percent_Identity=29.5336787564767, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI269784678, Length=193, Percent_Identity=29.5336787564767, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789148, Length=431, Percent_Identity=78.6542923433875, Blast_Score=712, Evalue=0.0,
Organism=Escherichia coli, GI1787083, Length=378, Percent_Identity=27.2486772486773, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1790403, Length=182, Percent_Identity=34.0659340659341, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24666579, Length=213, Percent_Identity=31.924882629108, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 48498; Mature: 48366

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQFYSAKRRVTTRQIITVTVNDLDPFGQGVARHQGKALFVSGVLPHEQAEVVLVEDKKQ
CCCHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEEEECHHH
YARAEVKRRLTDSPQRQAPRCPHFGVCGGCQQQHASVPLQQQSKRAALGRLMKREVDDVI
HHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AGAPWGYRRRARLSLNYQPKTQQLQMGFRQANAKAIVDVVQCPVLVPQLEALLPAVRECL
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SALSALRHLGHVELVQADNGPLMVLRHTAALPATDREKLERFSQTHGLSLYLAPQSEILE
HHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHH
HIHGEAPWYTSDGLRLVFSPRDFIQVNDGVNQQMVRTALEWLDLRPEDRVLDLFCGMGNF
HHCCCCCEEECCCCEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
TLPLATRAAHVVGVEGVPALVEKGRENAARNGLSNVTFFHENLEEDVTRQAWAKHGFDKV
EEHHHHHHHHEEECCCCHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
LLDPARAGAPGVMPHIIKLAPRRVVYVSCNPATLARDSETLLQAGYQIQRLAMLDMFPHT
EECCCCCCCCCHHHHHHHHCCCEEEEEECCCHHCCCCHHHHHHCCHHHHHHHHHHCCCCC
GHLESMVLFERRLT
CCHHHHHHHHHHCC
>Mature Secondary Structure 
AQFYSAKRRVTTRQIITVTVNDLDPFGQGVARHQGKALFVSGVLPHEQAEVVLVEDKKQ
CCHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEEEECHHH
YARAEVKRRLTDSPQRQAPRCPHFGVCGGCQQQHASVPLQQQSKRAALGRLMKREVDDVI
HHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AGAPWGYRRRARLSLNYQPKTQQLQMGFRQANAKAIVDVVQCPVLVPQLEALLPAVRECL
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
SALSALRHLGHVELVQADNGPLMVLRHTAALPATDREKLERFSQTHGLSLYLAPQSEILE
HHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHH
HIHGEAPWYTSDGLRLVFSPRDFIQVNDGVNQQMVRTALEWLDLRPEDRVLDLFCGMGNF
HHCCCCCEEECCCCEEEECCCCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCC
TLPLATRAAHVVGVEGVPALVEKGRENAARNGLSNVTFFHENLEEDVTRQAWAKHGFDKV
EEHHHHHHHHEEECCCCHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
LLDPARAGAPGVMPHIIKLAPRRVVYVSCNPATLARDSETLLQAGYQIQRLAMLDMFPHT
EECCCCCCCCCHHHHHHHHCCCEEEEEECCCHHCCCCHHHHHHCCHHHHHHHHHHCCCCC
GHLESMVLFERRLT
CCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA