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The map label for this gene is emrB [H]

Identifier: 238896139

GI number: 238896139

Start: 4109300

End: 4110835

Strand: Direct

Name: emrB [H]

Synonym: KP1_4280

Alternate gene names: 238896139

Gene position: 4109300-4110835 (Clockwise)

Preceding gene: 238896138

Following gene: 238896140

Centisome position: 78.29

GC content: 61.2

Gene sequence:

>1536_bases
ATGCAACAGCAAAAACCGCTGGAAGGCGCGCAGCTGGTCATTATGACCATTGCGCTGTCGCTCGCGACGTTCATGCAGGT
GCTGGATTCCACTATCGCCAACGTGGCGATCCCGACCATTGCCGGCAACCTTGGCTCGTCGCTGAGCCAGGGGACCTGGG
TCATCACCTCGTTCGGGGTCGCTAACGCCATCTCCATCCCGATCACCGGCTGGCTGGCCAAGCGCGTCGGCGAGGTGAAG
CTGTTCCTGTGGTCAACCACCGCCTTCGCCATTGCCTCCTGGGCCTGCGGCGTCTCCAACAGCCTGACGATGCTGATTTT
CTTCCGCGTCATTCAGGGGATCGTCGCCGGACCGCTGATCCCGCTGTCGCAAAGTCTGCTGCTCAGCAACTATCCCCCGG
CGAAGCGCTCTATCGCTCTGGCGCTGTGGTCAATGACGGTGATTGTCGCGCCCATCTGCGGTCCGATCCTCGGCGGCTAT
ATCAGCGATAACTATCACTGGGGCTGGATCTTCTTTATCAACGTGCCCATTGGCGTGGCGGTGGTGCTGATGACCCTGCA
GACCCTGCGCAACCGGGAAACCAAAACCGAACAGCGGCGGATCGACGGTGTCGGTCTGGCGCTGCTGATCATCGGTATCG
GTAGCCTGCAGGTGATGCTGGACCGGGGTAAAGAGCTGGACTGGTTCTCCTCCAGTGAGATTATCATTCTGACCATCGTG
GCGGTGGTGGCGATAAGCTTCCTGATCGTCTGGGAGCTAACCGACGATAACCCGATTGTCGATCTGTCGCTATTTAAGTC
GCGCAACTTCACCATCGGCTGCCTCTGTATCAGTCTGGCCTATATGCTCTACTTCGGCGCCATCGTACTGCTGCCGCAGC
TGTTGCAGGAGGTCTACGGCTACACCGCGACCTGGGCCGGCCTGGCGTCGGCGCCGGTGGGGGTGATCCCGGTCATTCTG
TCGCCGATTATCGGCCGCTTCGCGCATAAGCTCGACATGCGGCGGCTGGTCACCTTCAGCTTTATTATGTACGCGGTCTG
CTTCTACTGGCGCGCCTGGACCTTCGAACCGGGGATGGACTTTGGCGCCTCGGCGTGGCCACAGTTTATCCAGGGCTTCG
CGGTGGCCTGCTTCTTCATGCCGTTGACCACCATCACCCTCTCCGGCCTGCCGCCGGAGCGGCTGGCGGCGGCGTCGAGC
CTGTCGAACTTCACCCGTACCCTTGCCGGCTCCATCGGGACCTCGATCACCACCACCCTGTGGACCAACCGCGAGGCGCT
GCATCATGCGCAGCTCACCGAATCGGTGACCCCCTTTAACCCCAACGCGCAGCAGATATACGATCAGCTGCAGGGCATGG
GAATGACGCAGCAGCAGGCGTCCGGCTGGATAGCGCAGCAGATAACCAACCAGGGGCTGATTATCTCCGCCAACGAAATC
TTCTGGATTTCTGCGGCTATTTTCATCCTGCTGTTGGGGCTGGTGTGGTTCGCCAGACCACCGTTCAGCGCCGGCGGCGG
CGGCGGCGCGCACTAA

Upstream 100 bases:

>100_bases
GGTTTACGAGAGCAATGCCCGTGAAATCGCTCTCGATCCGGTTAATAAGCTGATAGACGAGATCATTCAGGCCAACGCCG
GATAATTCGGGGTGAGTGTA

Downstream 100 bases:

>100_bases
GCCTCACATAAAGGGACCTCCGGGTCCCTTTTTTACGGCGTCAGGTTGTCCAGTTCTCGCTAGTTTCTGTCTCGTTTGCG
AAAGCCCGTCACCCTGCGCT

Product: multidrug resistance membrane translocase

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPITGWLAKRVGEVK
LFLWSTTAFAIASWACGVSNSLTMLIFFRVIQGIVAGPLIPLSQSLLLSNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRNRETKTEQRRIDGVGLALLIIGIGSLQVMLDRGKELDWFSSSEIIILTIV
AVVAISFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGVIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTLWTNREALHHAQLTESVTPFNPNAQQIYDQLQGMGMTQQQASGWIAQQITNQGLIISANEI
FWISAAIFILLLGLVWFARPPFSAGGGGGAH

Sequences:

>Translated_511_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPITGWLAKRVGEVK
LFLWSTTAFAIASWACGVSNSLTMLIFFRVIQGIVAGPLIPLSQSLLLSNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRNRETKTEQRRIDGVGLALLIIGIGSLQVMLDRGKELDWFSSSEIIILTIV
AVVAISFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGVIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTLWTNREALHHAQLTESVTPFNPNAQQIYDQLQGMGMTQQQASGWIAQQITNQGLIISANEI
FWISAAIFILLLGLVWFARPPFSAGGGGGAH
>Mature_511_residues
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGVANAISIPITGWLAKRVGEVK
LFLWSTTAFAIASWACGVSNSLTMLIFFRVIQGIVAGPLIPLSQSLLLSNYPPAKRSIALALWSMTVIVAPICGPILGGY
ISDNYHWGWIFFINVPIGVAVVLMTLQTLRNRETKTEQRRIDGVGLALLIIGIGSLQVMLDRGKELDWFSSSEIIILTIV
AVVAISFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGVIPVIL
SPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASS
LSNFTRTLAGSIGTSITTTLWTNREALHHAQLTESVTPFNPNAQQIYDQLQGMGMTQQQASGWIAQQITNQGLIISANEI
FWISAAIFILLLGLVWFARPPFSAGGGGGAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=502, Percent_Identity=94.0239043824701, Blast_Score=937, Evalue=0.0,
Organism=Escherichia coli, GI1788710, Length=506, Percent_Identity=62.2529644268775, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1790195, Length=420, Percent_Identity=32.3809523809524, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=29.2079207920792, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=26.8235294117647, Blast_Score=122, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6322958, Length=535, Percent_Identity=21.6822429906542, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319770, Length=313, Percent_Identity=26.517571884984, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=31.3333333333333, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=137, Percent_Identity=29.1970802919708, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55583; Mature: 55583

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEECCC
ANAISIPITGWLAKRVGEVKLFLWSTTAFAIASWACGVSNSLTMLIFFRVIQGIVAGPLI
CCEEECCHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
PLSQSLLLSNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGVA
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHHHHH
VVLMTLQTLRNRETKTEQRRIDGVGLALLIIGIGSLQVMLDRGKELDWFSSSEIIILTIV
HHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCCHHHHHHH
AVVAISFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYG
HHHHHHHHEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YTATWAGLASAPVGVIPVILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMD
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSITTTL
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
WTNREALHHAQLTESVTPFNPNAQQIYDQLQGMGMTQQQASGWIAQQITNQGLIISANEI
ECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEECCHH
FWISAAIFILLLGLVWFARPPFSAGGGGGAH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNLGSSLSQGTWVITSFGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCEEEEEECCC
ANAISIPITGWLAKRVGEVKLFLWSTTAFAIASWACGVSNSLTMLIFFRVIQGIVAGPLI
CCEEECCHHHHHHHCCCCEEEEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCC
PLSQSLLLSNYPPAKRSIALALWSMTVIVAPICGPILGGYISDNYHWGWIFFINVPIGVA
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECHHHHH
VVLMTLQTLRNRETKTEQRRIDGVGLALLIIGIGSLQVMLDRGKELDWFSSSEIIILTIV
HHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCHHHHHHHCCCCCCCCCCCCHHHHHHH
AVVAISFLIVWELTDDNPIVDLSLFKSRNFTIGCLCISLAYMLYFGAIVLLPQLLQEVYG
HHHHHHHHEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
YTATWAGLASAPVGVIPVILSPIIGRFAHKLDMRRLVTFSFIMYAVCFYWRAWTFEPGMD
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FGASAWPQFIQGFAVACFFMPLTTITLSGLPPERLAAASSLSNFTRTLAGSIGTSITTTL
CCCHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
WTNREALHHAQLTESVTPFNPNAQQIYDQLQGMGMTQQQASGWIAQQITNQGLIISANEI
ECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHCCCCEEEECCHH
FWISAAIFILLLGLVWFARPPFSAGGGGGAH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]