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The map label for this gene is leuA [H]

Identifier: 238896140

GI number: 238896140

Start: 4110972

End: 4112648

Strand: Direct

Name: leuA [H]

Synonym: KP1_4281

Alternate gene names: 238896140

Gene position: 4110972-4112648 (Clockwise)

Preceding gene: 238896139

Following gene: 238896156

Centisome position: 78.33

GC content: 63.45

Gene sequence:

>1677_bases
ATGCTGAGCCATCCTGCTGAAAAGTACCGTCCCTATCCGCCGATCGCCCTGCCTGACCGCCGCTGGCCAGACCGGCAAAT
CAGCCACGCCCCGCGCTGGCTGTCGACTGACCTGCGCGACGGCAACCAGGCGCTGGCGGAGCCCATGGACAGCGCCCGCA
AACTGCAGTTCTGGGATCTGCTGCTGGAGTGCGGCTTTAAAGAGATTGAAGTCGCCTTCCCTTCCGCCTCACAAACCGAT
TTCAACTTTGTCCGCCAGCTGATTGACGAACAGCGCATCCCGGAGGATGTCACCATCCAGGTGCTGACCCAGGCGCGAGA
CCCGCTGATCCTGCGCACCTTTGAGGCGCTGCGCGGCGCCCGCCGGGCTACCGTCCACCTATACAATGCCACCGCGCCGC
TGTTTCGCGAGCTGGTTTTCGGCATGGACAAGGCCGAGGTGATCGCCCTCGCGACCCGCGCCACCCGGCTTATCCGTCAG
CAGTGCGAACAGCAGCCGGAGACCCGCTGGCAATATGAATATTCGCCTGAAACCTTCTGCTTCACCGAGCCCGAGTTTGC
GCTTGAGATTTGCGAAGCGGTGGCGGATGTCTGGCAGCCCTGCGCCGAGCGACCGATGATCGTCAATCTGCCGGCGACGG
TGGAAGTCAACACCCCCAACGTCTATGCCGATCAGATCGAGTATTTTTGCCGCCACTTCTCCCGCCGCGGCGAGGTCTGC
ATCAGCGTGCACCCGCATAACGATCGCGGCACCGGCGTCGCCAGCGCCGAGCTGGCGGTGATGGCCGGCGCCGATCGGGT
AGAAGGCTGCCTGTTTGGCAACGGCGAACGCACCGGCAACGTCTGTCTGGTGACTTTGGCGATGAACCTCTACAGCCAGG
GCATCGACCCTGAGCTTCGCTTCGAGCAGATGAACCGGGTGGTGGAGGTGGTGGAAAACTGCAACCAGATCCCCGTTCAC
CCGCGCCACCCGTGGGCGGGAAGTCTGGCTTACACCGCCTTCTCCGGCTCCCATCAGGATGCGATCAAAAAAGGGTTTGA
TGCCCGTCAGCCTGGCGATCCGTGGCAGATGCCCTACCTGCCCATTGACCCGCAGGATATAGGCTGCAGCTATGAGGCGG
TGATCCGCGTCAACAGCCAGTCGGGGAAGAGCGGCAGCGCCTGGCTTATCGAACAAAATCATGGTCTGAAACTGCCCCGC
GGGCTGCAGCAGGACTTCAGCCAGCACGTTCAGCAGGCCACCGATAGCGACGGCAAAGAGATGACCCACCATGCGCTGTG
GCAGCTGTTCCGCACGCGCTACGGGCTGCAGGCGCAGCCGGCGCTGACGCTGCTCGATTATCAGAGCGCCAGCCAGCAGG
ATGGCCAGCTTTCCCTGCAGGCAACGCTGCGTCACCACGGCGAAACCCGACGCCTGCAGGGCCAGGGCAACGGCCTGCTC
TCCGCCGCAGCCAGCGGGCTCAGCGCGCTGTTTCGGCAGCCGTTTATGATCAAGGACTATCATGAGCATACCCTCGGCGC
GCGCAGCGACAGCCGGTCGGTGGCCTATATCCGCTGCGTTTTTCCGCAAGGAGAAAGCTACTGGGGCGTGGGTATCGATA
ATGACGTGGCGCGCGCCTCGCTGCAGGCGCTGTGCAATGCGCTCAGCGCCGCCGATCAGGCCGGGGGACGAAAATAG

Upstream 100 bases:

>100_bases
GGCGTCAGGTTGTCCAGTTCTCGCTAGTTTCTGTCTCGTTTGCGAAAGCCCGTCACCCTGCGCTCGGGCAGGATAAAGGC
TGACCATTAAGGAGAACACT

Downstream 100 bases:

>100_bases
TCGCTGAGGGCGACGCCCATCACCCGACGAAACATGGCGCTGAAGGCGCTGTGGCTACCGTAGCCCAGCTCCAGCGCCAC
CCGCAGCACGGAGTGGCCCT

Product: putative isopropylmalate/homocitrate/ citramalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MLSHPAEKYRPYPPIALPDRRWPDRQISHAPRWLSTDLRDGNQALAEPMDSARKLQFWDLLLECGFKEIEVAFPSASQTD
FNFVRQLIDEQRIPEDVTIQVLTQARDPLILRTFEALRGARRATVHLYNATAPLFRELVFGMDKAEVIALATRATRLIRQ
QCEQQPETRWQYEYSPETFCFTEPEFALEICEAVADVWQPCAERPMIVNLPATVEVNTPNVYADQIEYFCRHFSRRGEVC
ISVHPHNDRGTGVASAELAVMAGADRVEGCLFGNGERTGNVCLVTLAMNLYSQGIDPELRFEQMNRVVEVVENCNQIPVH
PRHPWAGSLAYTAFSGSHQDAIKKGFDARQPGDPWQMPYLPIDPQDIGCSYEAVIRVNSQSGKSGSAWLIEQNHGLKLPR
GLQQDFSQHVQQATDSDGKEMTHHALWQLFRTRYGLQAQPALTLLDYQSASQQDGQLSLQATLRHHGETRRLQGQGNGLL
SAAASGLSALFRQPFMIKDYHEHTLGARSDSRSVAYIRCVFPQGESYWGVGIDNDVARASLQALCNALSAADQAGGRK

Sequences:

>Translated_558_residues
MLSHPAEKYRPYPPIALPDRRWPDRQISHAPRWLSTDLRDGNQALAEPMDSARKLQFWDLLLECGFKEIEVAFPSASQTD
FNFVRQLIDEQRIPEDVTIQVLTQARDPLILRTFEALRGARRATVHLYNATAPLFRELVFGMDKAEVIALATRATRLIRQ
QCEQQPETRWQYEYSPETFCFTEPEFALEICEAVADVWQPCAERPMIVNLPATVEVNTPNVYADQIEYFCRHFSRRGEVC
ISVHPHNDRGTGVASAELAVMAGADRVEGCLFGNGERTGNVCLVTLAMNLYSQGIDPELRFEQMNRVVEVVENCNQIPVH
PRHPWAGSLAYTAFSGSHQDAIKKGFDARQPGDPWQMPYLPIDPQDIGCSYEAVIRVNSQSGKSGSAWLIEQNHGLKLPR
GLQQDFSQHVQQATDSDGKEMTHHALWQLFRTRYGLQAQPALTLLDYQSASQQDGQLSLQATLRHHGETRRLQGQGNGLL
SAAASGLSALFRQPFMIKDYHEHTLGARSDSRSVAYIRCVFPQGESYWGVGIDNDVARASLQALCNALSAADQAGGRK
>Mature_558_residues
MLSHPAEKYRPYPPIALPDRRWPDRQISHAPRWLSTDLRDGNQALAEPMDSARKLQFWDLLLECGFKEIEVAFPSASQTD
FNFVRQLIDEQRIPEDVTIQVLTQARDPLILRTFEALRGARRATVHLYNATAPLFRELVFGMDKAEVIALATRATRLIRQ
QCEQQPETRWQYEYSPETFCFTEPEFALEICEAVADVWQPCAERPMIVNLPATVEVNTPNVYADQIEYFCRHFSRRGEVC
ISVHPHNDRGTGVASAELAVMAGADRVEGCLFGNGERTGNVCLVTLAMNLYSQGIDPELRFEQMNRVVEVVENCNQIPVH
PRHPWAGSLAYTAFSGSHQDAIKKGFDARQPGDPWQMPYLPIDPQDIGCSYEAVIRVNSQSGKSGSAWLIEQNHGLKLPR
GLQQDFSQHVQQATDSDGKEMTHHALWQLFRTRYGLQAQPALTLLDYQSASQQDGQLSLQATLRHHGETRRLQGQGNGLL
SAAASGLSALFRQPFMIKDYHEHTLGARSDSRSVAYIRCVFPQGESYWGVGIDNDVARASLQALCNALSAADQAGGRK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=521, Percent_Identity=23.4165067178503, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6324225, Length=570, Percent_Identity=49.2982456140351, Blast_Score=574, Evalue=1e-164,
Organism=Saccharomyces cerevisiae, GI6324682, Length=571, Percent_Identity=48.5113835376532, Blast_Score=569, Evalue=1e-163,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 62676; Mature: 62676

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSHPAEKYRPYPPIALPDRRWPDRQISHAPRWLSTDLRDGNQALAEPMDSARKLQFWDL
CCCCCCHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLECGFKEIEVAFPSASQTDFNFVRQLIDEQRIPEDVTIQVLTQARDPLILRTFEALRGA
HHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCC
RRATVHLYNATAPLFRELVFGMDKAEVIALATRATRLIRQQCEQQPETRWQYEYSPETFC
CEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEE
FTEPEFALEICEAVADVWQPCAERPMIVNLPATVEVNTPNVYADQIEYFCRHFSRRGEVC
ECCHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCCEE
ISVHPHNDRGTGVASAELAVMAGADRVEGCLFGNGERTGNVCLVTLAMNLYSQGIDPELR
EEEECCCCCCCCCCHHHEEEEECCCHHCEEEECCCCCCCCEEEEHHHHHHHHCCCCCCHH
FEQMNRVVEVVENCNQIPVHPRHPWAGSLAYTAFSGSHQDAIKKGFDARQPGDPWQMPYL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCC
PIDPQDIGCSYEAVIRVNSQSGKSGSAWLIEQNHGLKLPRGLQQDFSQHVQQATDSDGKE
CCCHHHCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHH
MTHHALWQLFRTRYGLQAQPALTLLDYQSASQQDGQLSLQATLRHHGETRRLQGQGNGLL
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEHHHHHHHCCCCEEECCCCCCHH
SAAASGLSALFRQPFMIKDYHEHTLGARSDSRSVAYIRCVFPQGESYWGVGIDNDVARAS
HHHHHHHHHHHHCCCEEECHHHHHCCCCCCCCCEEEEEEEECCCCCEECCCCCCHHHHHH
LQALCNALSAADQAGGRK
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSHPAEKYRPYPPIALPDRRWPDRQISHAPRWLSTDLRDGNQALAEPMDSARKLQFWDL
CCCCCCHHCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLECGFKEIEVAFPSASQTDFNFVRQLIDEQRIPEDVTIQVLTQARDPLILRTFEALRGA
HHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHCCC
RRATVHLYNATAPLFRELVFGMDKAEVIALATRATRLIRQQCEQQPETRWQYEYSPETFC
CEEEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEE
FTEPEFALEICEAVADVWQPCAERPMIVNLPATVEVNTPNVYADQIEYFCRHFSRRGEVC
ECCHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCCEE
ISVHPHNDRGTGVASAELAVMAGADRVEGCLFGNGERTGNVCLVTLAMNLYSQGIDPELR
EEEECCCCCCCCCCHHHEEEEECCCHHCEEEECCCCCCCCEEEEHHHHHHHHCCCCCCHH
FEQMNRVVEVVENCNQIPVHPRHPWAGSLAYTAFSGSHQDAIKKGFDARQPGDPWQMPYL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCCCCC
PIDPQDIGCSYEAVIRVNSQSGKSGSAWLIEQNHGLKLPRGLQQDFSQHVQQATDSDGKE
CCCHHHCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHH
MTHHALWQLFRTRYGLQAQPALTLLDYQSASQQDGQLSLQATLRHHGETRRLQGQGNGLL
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCEEHHHHHHHCCCCEEECCCCCCHH
SAAASGLSALFRQPFMIKDYHEHTLGARSDSRSVAYIRCVFPQGESYWGVGIDNDVARAS
HHHHHHHHHHHHCCCEEECHHHHHCCCCCCCCCEEEEEEEECCCCCEECCCCCCHHHHHH
LQALCNALSAADQAGGRK
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA