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The map label for this gene is mnmC [H]

Identifier: 238895845

GI number: 238895845

Start: 3788342

End: 3790390

Strand: Direct

Name: mnmC [H]

Synonym: KP1_3964

Alternate gene names: 238895845

Gene position: 3788342-3790390 (Clockwise)

Preceding gene: 238895842

Following gene: 238895852

Centisome position: 72.18

GC content: 62.76

Gene sequence:

>2049_bases
TTGCGCCCTCAGGCACACATCGTTAAGATCGTGCTACGCCTTGTGATTCGAGTAACTTACGTGAAACAAAACGCCATACA
ACCCGCCAACCTGGAATTCAACGCTGAGGGTACACCTGTTTCCCGAGATTTCGACGATGTTTACTTTTCCAATGACAACG
GTCTGGAAGAGACTCGCTACGTTTTTCTCGGCGGAAATCGCCTGCCGGAGCGCTTCCCCAGCCACCCTCGCCCGCTGATG
ATCATCGCGGAGAGCGGCTTCGGCACCGGGCTCAATTTCCTCACCCTCTGGCAGGCGTTTGACGTTTTTGTTCGTGATAA
CCCGGACGTTACCTTGCAAAGGTTACATTTCATCAGTTTTGAAAAATATCCGCTGAAGGCTGAGGATCTCCGCCTGGCGC
ACCAGCGCTGGCCAGAGCTGGCTCCGTGGGCGCAGCAGCTGCAGGCGCAATGGCCGTCGGCCTTCGGCGGGTGCCATCGT
CTGCTGCTCGACGGCGGGCGGGTGACGCTCGATCTGTGGTTTGGCGATATCAATGAGCTGACCAGGGAGCTGGATGATTC
GCTGAACCAGCAGGTGGACGCCTGGTTTCTTGACGGCTTTGCCCCGGCGAAAAATCCCGACATGTGGACTCAGGATCTGT
TCAGCGCTATGGCGCGCCTGGCGCGACCCGGCGGGACGCTGGCCACCTTTACCTCCGCCGGTTTTGTCCGCCGTGGCCTG
CAGGAGGCCGGCTTTACCATGCGTAAAAGCAAAGGCTTCGGCCGCAAGCGGGAGATGCTGACCGGCGAGATGGCGCAAAC
GCTGAGCTTTCCTGCGCGCGCCCCCTGGTTCGCTCGCAGTAGCAGCGATGCCCGCGAGGCGGCCATCATCGGCGGCGGGA
TCGCCAGCGCCCTGCTCTCCCTGGCGCTGCTGCGCCGCGGCTGGCAGGTGACCCTTTACTGCGCCGATGAGGCTCCAGCG
CAGGGCGCCTCCGGCAATCGCCAGGGCGCGCTGTATCCGCTGTTAAGTCAGCACGATCCGGCGCTGGCGCGCTTTTTTCC
GGCCGCCTTTACCTTCGCCCGCCGAATGTATGACGCGCTGCCGGTGATGTTCGATCACCAATGGTGCGGTGTTACCCAGC
TCGGCTGGGATGAGAAAAGCACGCATAAAATCGCCCAGATGCTGGCGCTGAATTTACCCCCGGACATCGCCTGCGCGGTG
ACGGCGGAACAGGTTGCCGGGCTGACCGGCGTCGATACCGGCTGCGGCGGCATCACCTATCCGGCCGGCGGCTGGCTGTG
CCCGCAGCAGCTCACCGCCGAGCTGCTGGCGCTGGCGGCCACGCGCGGACTGCACGTGCACTATGGTTATCCCGTCGAAA
CGCTCAGCGCGGAGGGCGATGGCTGGCTGCTCAATCAGCAGCGCTATCATCAGGCGGTGGTTCTGGCGAACGGCCACCGC
ATCACTGGTTTTGCGCAAACCGCCCAACTGCCGGTCTACCCGGTTGGCGGCCAGGTCAGCCATATTCCCACCACCCCGCG
GCTTGCCGCCCTGCGCCAGGTGCTGTGCTACGACGGCTACCTGACGCCACAGAATCCGCAAAATCAGCAGCACTGCATTG
GCGCCAGCTACCACCGCGGGAAAACCGACACCACCTTCAGCGAAGAAGATCAGCAGCACAACCGGCAACGGCTGATCGAC
TGCTTCCCGGGCGCGGAGTGGCCACAGGACGTGGATATCAGCGCCAACGACGCTCGCTGCGGCGTGCGCTGCGCCACCCG
CGATCATCTACCGATGGTCGGCAACGTGCCGGACTACGCGGCAACATTGACGCAATACGCCAGCCTCCACGAACAACCGG
ATATCGCCGATAGCGCGCCGGTCTGTCGTAACCTGTTTATGCTTGGCGCGCTCGGGTCGCGCGGACTTTGCACCGCGCCG
TTAAGCGCTGAGCTGCTGGCGGCGCAAATGAGCGCGGAACCGCTGCCGCTGGATAGCGACACCCTGGCGGCGCTGAATCC
GAATCGGCTGTGGGTGAGAAAACTGCTGAAGGGAAAAGCGGTGAAATAA

Upstream 100 bases:

>100_bases
ATTAAATACCTCTGTAAGTCGCACTATTTTAAGTTTCGTGTCGCACAATAGCGTACACTTGTACGCCGAACAAGTCCGAT
CAGACATTTTGTGTATAAAT

Downstream 100 bases:

>100_bases
AACCATGAGGGCAGGCAATTTCCCGGGTACGGCTGACGCCTGACCCGGGCTACAACATGGCTCAGGCCTGATTTTTCGCC
TGTTGATACAGGTTATGCCA

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 682; Mature: 682

Protein sequence:

>682_residues
MRPQAHIVKIVLRLVIRVTYVKQNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLPERFPSHPRPLM
IIAESGFGTGLNFLTLWQAFDVFVRDNPDVTLQRLHFISFEKYPLKAEDLRLAHQRWPELAPWAQQLQAQWPSAFGGCHR
LLLDGGRVTLDLWFGDINELTRELDDSLNQQVDAWFLDGFAPAKNPDMWTQDLFSAMARLARPGGTLATFTSAGFVRRGL
QEAGFTMRKSKGFGRKREMLTGEMAQTLSFPARAPWFARSSSDAREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
QGASGNRQGALYPLLSQHDPALARFFPAAFTFARRMYDALPVMFDHQWCGVTQLGWDEKSTHKIAQMLALNLPPDIACAV
TAEQVAGLTGVDTGCGGITYPAGGWLCPQQLTAELLALAATRGLHVHYGYPVETLSAEGDGWLLNQQRYHQAVVLANGHR
ITGFAQTAQLPVYPVGGQVSHIPTTPRLAALRQVLCYDGYLTPQNPQNQQHCIGASYHRGKTDTTFSEEDQQHNRQRLID
CFPGAEWPQDVDISANDARCGVRCATRDHLPMVGNVPDYAATLTQYASLHEQPDIADSAPVCRNLFMLGALGSRGLCTAP
LSAELLAAQMSAEPLPLDSDTLAALNPNRLWVRKLLKGKAVK

Sequences:

>Translated_682_residues
MRPQAHIVKIVLRLVIRVTYVKQNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLPERFPSHPRPLM
IIAESGFGTGLNFLTLWQAFDVFVRDNPDVTLQRLHFISFEKYPLKAEDLRLAHQRWPELAPWAQQLQAQWPSAFGGCHR
LLLDGGRVTLDLWFGDINELTRELDDSLNQQVDAWFLDGFAPAKNPDMWTQDLFSAMARLARPGGTLATFTSAGFVRRGL
QEAGFTMRKSKGFGRKREMLTGEMAQTLSFPARAPWFARSSSDAREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
QGASGNRQGALYPLLSQHDPALARFFPAAFTFARRMYDALPVMFDHQWCGVTQLGWDEKSTHKIAQMLALNLPPDIACAV
TAEQVAGLTGVDTGCGGITYPAGGWLCPQQLTAELLALAATRGLHVHYGYPVETLSAEGDGWLLNQQRYHQAVVLANGHR
ITGFAQTAQLPVYPVGGQVSHIPTTPRLAALRQVLCYDGYLTPQNPQNQQHCIGASYHRGKTDTTFSEEDQQHNRQRLID
CFPGAEWPQDVDISANDARCGVRCATRDHLPMVGNVPDYAATLTQYASLHEQPDIADSAPVCRNLFMLGALGSRGLCTAP
LSAELLAAQMSAEPLPLDSDTLAALNPNRLWVRKLLKGKAVK
>Mature_682_residues
MRPQAHIVKIVLRLVIRVTYVKQNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNRLPERFPSHPRPLM
IIAESGFGTGLNFLTLWQAFDVFVRDNPDVTLQRLHFISFEKYPLKAEDLRLAHQRWPELAPWAQQLQAQWPSAFGGCHR
LLLDGGRVTLDLWFGDINELTRELDDSLNQQVDAWFLDGFAPAKNPDMWTQDLFSAMARLARPGGTLATFTSAGFVRRGL
QEAGFTMRKSKGFGRKREMLTGEMAQTLSFPARAPWFARSSSDAREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
QGASGNRQGALYPLLSQHDPALARFFPAAFTFARRMYDALPVMFDHQWCGVTQLGWDEKSTHKIAQMLALNLPPDIACAV
TAEQVAGLTGVDTGCGGITYPAGGWLCPQQLTAELLALAATRGLHVHYGYPVETLSAEGDGWLLNQQRYHQAVVLANGHR
ITGFAQTAQLPVYPVGGQVSHIPTTPRLAALRQVLCYDGYLTPQNPQNQQHCIGASYHRGKTDTTFSEEDQQHNRQRLID
CFPGAEWPQDVDISANDARCGVRCATRDHLPMVGNVPDYAATLTQYASLHEQPDIADSAPVCRNLFMLGALGSRGLCTAP
LSAELLAAQMSAEPLPLDSDTLAALNPNRLWVRKLLKGKAVK

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=666, Percent_Identity=76.4264264264264, Blast_Score=1037, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 75262; Mature: 75262

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPQAHIVKIVLRLVIRVTYVKQNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRY
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCEEE
VFLGGNRLPERFPSHPRPLMIIAESGFGTGLNFLTLWQAFDVFVRDNPDVTLQRLHFISF
EEECCCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHEEECCCCCCEEEHHEEHCC
EKYPLKAEDLRLAHQRWPELAPWAQQLQAQWPSAFGGCHRLLLDGGRVTLDLWFGDINEL
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHEECCCEEEEEEECCCHHHH
TRELDDSLNQQVDAWFLDGFAPAKNPDMWTQDLFSAMARLARPGGTLATFTSAGFVRRGL
HHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
QEAGFTMRKSKGFGRKREMLTGEMAQTLSFPARAPWFARSSSDAREAAIIGGGIASALLS
HHHCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEECCHHHHHHHH
LALLRRGWQVTLYCADEAPAQGASGNRQGALYPLLSQHDPALARFFPAAFTFARRMYDAL
HHHHHCCCEEEEEECCCCCCCCCCCCCCCCEECHHCCCCHHHHHHHHHHHHHHHHHHHHC
PVMFDHQWCGVTQLGWDEKSTHKIAQMLALNLPPDIACAVTAEQVAGLTGVDTGCGGITY
CCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCCCCCC
PAGGWLCPQQLTAELLALAATRGLHVHYGYPVETLSAEGDGWLLNQQRYHQAVVLANGHR
CCCCCCCHHHHHHHHHHHHHHCCEEEEECCCHHEECCCCCCEEECHHHHCEEEEEECCCE
ITGFAQTAQLPVYPVGGQVSHIPTTPRLAALRQVLCYDGYLTPQNPQNQQHCIGASYHRG
EEEEHHHCCCCEEECCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCC
KTDTTFSEEDQQHNRQRLIDCFPGAEWPQDVDISANDARCGVRCATRDHLPMVGNVPDYA
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHH
ATLTQYASLHEQPDIADSAPVCRNLFMLGALGSRGLCTAPLSAELLAAQMSAEPLPLDSD
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHCCCCCCCCCC
TLAALNPNRLWVRKLLKGKAVK
CEEEECCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MRPQAHIVKIVLRLVIRVTYVKQNAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRY
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCEEE
VFLGGNRLPERFPSHPRPLMIIAESGFGTGLNFLTLWQAFDVFVRDNPDVTLQRLHFISF
EEECCCCCHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHEEECCCCCCEEEHHEEHCC
EKYPLKAEDLRLAHQRWPELAPWAQQLQAQWPSAFGGCHRLLLDGGRVTLDLWFGDINEL
CCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHEECCCEEEEEEECCCHHHH
TRELDDSLNQQVDAWFLDGFAPAKNPDMWTQDLFSAMARLARPGGTLATFTSAGFVRRGL
HHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHH
QEAGFTMRKSKGFGRKREMLTGEMAQTLSFPARAPWFARSSSDAREAAIIGGGIASALLS
HHHCCEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHEEECCHHHHHHHH
LALLRRGWQVTLYCADEAPAQGASGNRQGALYPLLSQHDPALARFFPAAFTFARRMYDAL
HHHHHCCCEEEEEECCCCCCCCCCCCCCCCEECHHCCCCHHHHHHHHHHHHHHHHHHHHC
PVMFDHQWCGVTQLGWDEKSTHKIAQMLALNLPPDIACAVTAEQVAGLTGVDTGCGGITY
CCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCCCCCCCC
PAGGWLCPQQLTAELLALAATRGLHVHYGYPVETLSAEGDGWLLNQQRYHQAVVLANGHR
CCCCCCCHHHHHHHHHHHHHHCCEEEEECCCHHEECCCCCCEEECHHHHCEEEEEECCCE
ITGFAQTAQLPVYPVGGQVSHIPTTPRLAALRQVLCYDGYLTPQNPQNQQHCIGASYHRG
EEEEHHHCCCCEEECCCCEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCC
KTDTTFSEEDQQHNRQRLIDCFPGAEWPQDVDISANDARCGVRCATRDHLPMVGNVPDYA
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHH
ATLTQYASLHEQPDIADSAPVCRNLFMLGALGSRGLCTAPLSAELLAAQMSAEPLPLDSD
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHCCCCCCCCCC
TLAALNPNRLWVRKLLKGKAVK
CEEEECCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA