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The map label for this gene is tdcE [H]

Identifier: 238895346

GI number: 238895346

Start: 3231057

End: 3233351

Strand: Reverse

Name: tdcE [H]

Synonym: KP1_3408

Alternate gene names: 238895346

Gene position: 3233351-3231057 (Counterclockwise)

Preceding gene: 238895347

Following gene: 238895345

Centisome position: 61.61

GC content: 62.05

Gene sequence:

>2295_bases
ATGAAAGTAGATATCGATACCCAGGATGTACGATACGCTGACGCCTGGCTGGGCTTTCGCGGTACGGCGTGGCAAACGCA
GATCGACGTTCGCGACTTTATTCAGCATAACTACACCCCTTACGAGGGCGATGAGTCTTTCCTCGCCGACGCGACGCCAG
CGACCACCGCCCTCTGGGAGCAGGTGATGGCCGGGATCCGCGTCGAGAACGCCACCCATGCGCCGGTGGATTTCGACACC
AATGTCGCCACCTCCATCACCGCCCACGCGGCGGGCTATATCAACCAGCCGCTGGAAAAGATCGTCGGCCTGCAAACGGA
CCAGCCGCTGAAGCGCGCCCTGCATCCTTTTGGCGGCATCAAGATGATCAAAAGCGCCTTCGAGGCCTATGGTCGCGAAA
TGGATCCTGACTTTGAGTATCAGTTTACGGCGCTGCGTAAGACCCACAATCAGGGCGTTTTTGACGTCTATTCCCCGGAT
ATGCTGCGCTGCCGCAAATCCGGGGTGCTGACCGGGCTACCGGACGGCTATGGCCGCGGACGGATCATCGGCGATTACCG
CCGCGTGGCGCTGTACGGCATTCGCTACCTGGTCCGCGAACGTGAGCTGCAGTTTGCCGACCTGCAGCCGGCCCTTGAGC
GCGGCGAGGCGCTGGAGGCCACCCTGCGCCTGCGCGAGGAGCTGGCCGAACAGCGGCGCGCGCTGCTGCAGATGCAGGAG
ATGGCGGCCCGCTACGGCTGCGATATCTCTCACCCGGCGCGCACTGCCCGGGAGGCGGTGCAGTGGCTCTACTTCGCCTA
CCTGGCGGCGGTGAAGTCGCAGAACGGCGGCGCCATGTCGCTGGGGCGCACGGCCACCTTCCTCGACATCTATATCGAAC
GCGATCTGCGCGCCGGTTTATTAAATGAGGAGCAGGCCCAGGAGCTCATTGATCACTTCATCATGAAGATCCGCATGGTG
CGTTTCCTGCGCACCCCGGAATTTGATTCGCTGTTCTCCGGGGATCCCATCTGGGCGACGGAGGTGCTGGGCGGCATGGG
GCTGGATGGTCGCACCCTGGTCAGCAAAACCACCTTCCGCTATCTGCATACTCTGCATACCATGGGCCCGGCGCCGGAGC
CGAACCTGACCGTGCTCTGGTCGCAAGCGCTGCCAGTGGCGTTTAAAAAATATGCCGCCCGGGTCTCTATCGCCACCTCG
TCGCTGCAGTATGAGAACGACGATCTGATGCGCAGCGATTTTCACAGCGACGACTACGCCATCGCCTGCTGCGTCAGCCC
GATGGTGATCGGCAAGCAGATGCAGTTCTTCGGCGCCCGCGCCAATCTCGCCAAAACGCTGCTCTACGCGATTAACGGCG
GGGTGGATGAGAAACTGAAGATTCAGGTGGGGCCGAAAACCGCACCGTTGCGCGATGAGGTGCTGGACTACGGCACCGTG
ATGGCCAGCCTCGACCACTTTATGGACTGGCTGGCGGTGCAGTACATCAGCGCGCTGAACATCATCCATTGCATGCATGA
TAAGTACAGCTACGAGGCGGCGCTGATGGCCCTGCACGACCGCGACGTCTACCGCACGATGGCCTGCGGCATCGCCGGCC
TGTCGGTGGCGGCGGATTCGTTGTCGGCGATAAAGTACGCCCGGGTGAAGCCGGTGCGCGATCACCACGGGCTGGCGGTG
GACTTTGTCATTGAAGGCGACTATCCGCAGTACGGCAACAACGACGATCGGGTCGATGCCATCGCCTGCGACCTGGTGGA
GCGCTTTATGCGCAAAATTCAGGCGCTCCCCACCTGGCGTCAGGCGGTGCCGACCCAGTCGATCCTGACCATCACCTCCA
ACGTGGTATACGGCCAGAAAACCGGCAACACCCCGGACGGACGTCGGGCGGGGACGCCGTTCGCTCCGGGGGCCAATCCG
ATGCATGGCCGCGACCGGAAAGGGGCGGTGGCGTCGCTGACCTCGGTGGCGAAACTGCCGTTTACCTACGCCAAAGATGG
CATCTCGTATACCTTCTCCATCGTGCCGGCGGCGCTCGGCAAAGCCCCGTCAGCGCAGGAAAACAACCTGGTGGGCCTGC
TGGACGGCTACTTCCACCACGAGGAGACGGTGGAGGGGGGACAGCATCTCAACGTCAATGTGCTGAACCGGGAAAAGCTG
CTCGATGCGATAGAACACCCGGAGCAGTATCCGAATCTGACGATCCGCGTCTCCGGCTATGCGGTCCGCTTTAACGCGCT
GACCCGCGAACAGCAGCAGGATGTGATCTCCCGAACGTTCACCAGTCAACTCTGA

Upstream 100 bases:

>100_bases
AATGAAGAGCGCATGATTGCCCTCGATGCGATCCGCTTAGGCCGGATCCACACCGCGGCGGCGCTGGCCTGACTTTTTAT
TCTGTAGAGAGACGATGTTC

Downstream 100 bases:

>100_bases
GCGGCCACCGGGTCACAGGAGAGCCTTTAACGGGCTCTCCTGTTATCATCTTCACATTACCGCAACAAAATAATCACTAC
TGTTGATCATATTCGCATTT

Product: formate C-acetyltransferase

Products: CoA; pyruvate

Alternate protein names: Keto-acid formate-lyase [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MKVDIDTQDVRYADAWLGFRGTAWQTQIDVRDFIQHNYTPYEGDESFLADATPATTALWEQVMAGIRVENATHAPVDFDT
NVATSITAHAAGYINQPLEKIVGLQTDQPLKRALHPFGGIKMIKSAFEAYGREMDPDFEYQFTALRKTHNQGVFDVYSPD
MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQPALERGEALEATLRLREELAEQRRALLQMQE
MAARYGCDISHPARTAREAVQWLYFAYLAAVKSQNGGAMSLGRTATFLDIYIERDLRAGLLNEEQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVLGGMGLDGRTLVSKTTFRYLHTLHTMGPAPEPNLTVLWSQALPVAFKKYAARVSIATS
SLQYENDDLMRSDFHSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLRDEVLDYGTV
MASLDHFMDWLAVQYISALNIIHCMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYARVKPVRDHHGLAV
DFVIEGDYPQYGNNDDRVDAIACDLVERFMRKIQALPTWRQAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKAPSAQENNLVGLLDGYFHHEETVEGGQHLNVNVLNREKL
LDAIEHPEQYPNLTIRVSGYAVRFNALTREQQQDVISRTFTSQL

Sequences:

>Translated_764_residues
MKVDIDTQDVRYADAWLGFRGTAWQTQIDVRDFIQHNYTPYEGDESFLADATPATTALWEQVMAGIRVENATHAPVDFDT
NVATSITAHAAGYINQPLEKIVGLQTDQPLKRALHPFGGIKMIKSAFEAYGREMDPDFEYQFTALRKTHNQGVFDVYSPD
MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQPALERGEALEATLRLREELAEQRRALLQMQE
MAARYGCDISHPARTAREAVQWLYFAYLAAVKSQNGGAMSLGRTATFLDIYIERDLRAGLLNEEQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVLGGMGLDGRTLVSKTTFRYLHTLHTMGPAPEPNLTVLWSQALPVAFKKYAARVSIATS
SLQYENDDLMRSDFHSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLRDEVLDYGTV
MASLDHFMDWLAVQYISALNIIHCMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYARVKPVRDHHGLAV
DFVIEGDYPQYGNNDDRVDAIACDLVERFMRKIQALPTWRQAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKAPSAQENNLVGLLDGYFHHEETVEGGQHLNVNVLNREKL
LDAIEHPEQYPNLTIRVSGYAVRFNALTREQQQDVISRTFTSQL
>Mature_764_residues
MKVDIDTQDVRYADAWLGFRGTAWQTQIDVRDFIQHNYTPYEGDESFLADATPATTALWEQVMAGIRVENATHAPVDFDT
NVATSITAHAAGYINQPLEKIVGLQTDQPLKRALHPFGGIKMIKSAFEAYGREMDPDFEYQFTALRKTHNQGVFDVYSPD
MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQPALERGEALEATLRLREELAEQRRALLQMQE
MAARYGCDISHPARTAREAVQWLYFAYLAAVKSQNGGAMSLGRTATFLDIYIERDLRAGLLNEEQAQELIDHFIMKIRMV
RFLRTPEFDSLFSGDPIWATEVLGGMGLDGRTLVSKTTFRYLHTLHTMGPAPEPNLTVLWSQALPVAFKKYAARVSIATS
SLQYENDDLMRSDFHSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLRDEVLDYGTV
MASLDHFMDWLAVQYISALNIIHCMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADSLSAIKYARVKPVRDHHGLAV
DFVIEGDYPQYGNNDDRVDAIACDLVERFMRKIQALPTWRQAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANP
MHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKAPSAQENNLVGLLDGYFHHEETVEGGQHLNVNVLNREKL
LDAIEHPEQYPNLTIRVSGYAVRFNALTREQQQDVISRTFTSQL

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI48994926, Length=764, Percent_Identity=88.0890052356021, Blast_Score=1422, Evalue=0.0,
Organism=Escherichia coli, GI1787131, Length=754, Percent_Identity=75.1989389920424, Blast_Score=1230, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=625, Percent_Identity=27.36, Blast_Score=172, Evalue=6e-44,
Organism=Escherichia coli, GI1790388, Length=642, Percent_Identity=26.791277258567, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=76.5625, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: 2.3.1.54

Molecular weight: Translated: 85443; Mature: 85443

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVDIDTQDVRYADAWLGFRGTAWQTQIDVRDFIQHNYTPYEGDESFLADATPATTALWE
CCCCCCCCCCCHHHHHCCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
QVMAGIRVENATHAPVDFDTNVATSITAHAAGYINQPLEKIVGLQTDQPLKRALHPFGGI
HHHHCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHH
KMIKSAFEAYGREMDPDFEYQFTALRKTHNQGVFDVYSPDMLRCRKSGVLTGLPDGYGRG
HHHHHHHHHHCCCCCCCCCEEHHHHHHHCCCCEEECCCCHHHHHHHCCCCCCCCCCCCCC
RIIGDYRRVALYGIRYLVRERELQFADLQPALERGEALEATLRLREELAEQRRALLQMQE
CEEHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
MAARYGCDISHPARTAREAVQWLYFAYLAAVKSQNGGAMSLGRTATFLDIYIERDLRAGL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEEECHHHCCC
LNEEQAQELIDHFIMKIRMVRFLRTPEFDSLFSGDPIWATEVLGGMGLDGRTLVSKTTFR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHH
YLHTLHTMGPAPEPNLTVLWSQALPVAFKKYAARVSIATSSLQYENDDLMRSDFHSDDYA
HHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHEEEHHHCCCCCCHHHHHCCCCCCCE
IACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLRDEVLDYGTV
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHH
MASLDHFMDWLAVQYISALNIIHCMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSAIKYARVKPVRDHHGLAVDFVIEGDYPQYGNNDDRVDAIACDLVERFMRKIQALPTWR
HHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
QAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLP
HHCCCCHHHEEHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
FTYAKDGISYTFSIVPAALGKAPSAQENNLVGLLDGYFHHEETVEGGQHLNVNVLNREKL
CHHHCCCCEEEHHHHHHHHCCCCCCCCCCEEEEEHHHHCCHHHHCCCCEEEEEECCHHHH
LDAIEHPEQYPNLTIRVSGYAVRFNALTREQQQDVISRTFTSQL
HHHHHCHHHCCCEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVDIDTQDVRYADAWLGFRGTAWQTQIDVRDFIQHNYTPYEGDESFLADATPATTALWE
CCCCCCCCCCCHHHHHCCCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHH
QVMAGIRVENATHAPVDFDTNVATSITAHAAGYINQPLEKIVGLQTDQPLKRALHPFGGI
HHHHCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHH
KMIKSAFEAYGREMDPDFEYQFTALRKTHNQGVFDVYSPDMLRCRKSGVLTGLPDGYGRG
HHHHHHHHHHCCCCCCCCCEEHHHHHHHCCCCEEECCCCHHHHHHHCCCCCCCCCCCCCC
RIIGDYRRVALYGIRYLVRERELQFADLQPALERGEALEATLRLREELAEQRRALLQMQE
CEEHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
MAARYGCDISHPARTAREAVQWLYFAYLAAVKSQNGGAMSLGRTATFLDIYIERDLRAGL
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEEEECHHHCCC
LNEEQAQELIDHFIMKIRMVRFLRTPEFDSLFSGDPIWATEVLGGMGLDGRTLVSKTTFR
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHCCCCCCCCHHHHHHHHH
YLHTLHTMGPAPEPNLTVLWSQALPVAFKKYAARVSIATSSLQYENDDLMRSDFHSDDYA
HHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHEEEHHHCCCCCCHHHHHCCCCCCCE
IACCVSPMVIGKQMQFFGARANLAKTLLYAINGGVDEKLKIQVGPKTAPLRDEVLDYGTV
EEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHH
MASLDHFMDWLAVQYISALNIIHCMHDKYSYEAALMALHDRDVYRTMACGIAGLSVAADS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSAIKYARVKPVRDHHGLAVDFVIEGDYPQYGNNDDRVDAIACDLVERFMRKIQALPTWR
HHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
QAVPTQSILTITSNVVYGQKTGNTPDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLP
HHCCCCHHHEEHHCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
FTYAKDGISYTFSIVPAALGKAPSAQENNLVGLLDGYFHHEETVEGGQHLNVNVLNREKL
CHHHCCCCEEEHHHHHHHHCCCCCCCCCCEEEEEHHHHCCHHHHCCCCEEEEEECCHHHH
LDAIEHPEQYPNLTIRVSGYAVRFNALTREQQQDVISRTFTSQL
HHHHHCHHHCCCEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; formate

Specific reaction: acetyl-CoA + formate = CoA + pyruvate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 9484901 [H]