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The map label for this gene is livJ [C]

Identifier: 238895345

GI number: 238895345

Start: 3229511

End: 3230626

Strand: Reverse

Name: livJ [C]

Synonym: KP1_3407

Alternate gene names: 238895345

Gene position: 3230626-3229511 (Counterclockwise)

Preceding gene: 238895346

Following gene: 238895344

Centisome position: 61.55

GC content: 61.11

Gene sequence:

>1116_bases
ATGTCTTTGAAACTGAAAACAATGACGCTTCATGTTGTGATAGCAGGTTGTATGAGCATGGCCTTTTACGCCCAGGCAGA
CGTGAAGATCGGCGTCGCCGGGCCCTTTACCGGCCCCAACGCCACCTACGGCGCCCAGTACTGGAAGGGGGCCTCGCAGG
CGGTGGCCGATATCAATGCCGCCGGCGGCATCAAAGGCGAAAAGATTGTGCTGGTGCAGGGGGATGACGCGTGCGAACCG
AAGCAGGCGGTGGCGGTGGCCAACCGGCTGGTGGATGAAGCGAAAGTCTCCGCCGTGGTGGGGCACTTTTGCTCTTCCTC
AACGATGCCGGCCTCCGAGGTATATGACGAAGCCGGGATCCTGACCATTACCCCCGGCTCGACCAACCCGCAGATCACCG
AGCGCGGCATCAAAGATCTCTTCCGCATGTGTGGTCGTGACGATCAGCAGGGGGCGATCGCCGCCAACTATATGCTGGAC
GTGCTGAAGGCGAAGAAAATCGCCGTCATCCACGATAAAGACACCTATGGTCAGGGGCTGGCCGATGCCACCCGCGCGGC
GCTGGCGAAGCGGGGTACGAAAGAAGTGTTGTACGAAGGCCTTTCCCGCGGTGAAAAAGACTTTAACGCCCTGGTGACCA
AGATCGGCGCCCTCAAGCCGGACGTGGTCTACTTTGGCGGCTGCCATCCTGAAGCGGGCCCGCTGGTGCGCCAGATGCGC
GAGCAGGGGGTGCAGGCCAAATTCTTCTCCGGCGACTGTATCGTCACCGAGGAGCTGGTCACGGCCGCCGGCGGGCCGCA
GTTCACTAACGGCGTGCTGATGACCTTTGGTCAGGATCCGCGCACCCTCCCCGACGGCAAAGCGGTGATCGAGAAGTTCC
GCGCCAGCGGCTTTGAGCCGGAAGGTTATACCCTCTACGCCTACGCCTCCATTCAGGCTATCGCCGCGGCCTGGAACGCC
GTCGGTACCGATAACGCCAAAGCCAGCGACTGGCTGAAGAGCCACGACGTCGAGACGGTGATGGGCAAGAAAGCCTGGGA
TGGCAAAGGCGACCTCAAGGTGTCCGACTACGTGGTCTATCAGTGGGACGACAAGGGCAAATATCACCAGCTCTGA

Upstream 100 bases:

>100_bases
TGGCCGTCCTGGCGCTGCGCCAGCGTCGGCACCACACGGTAAGTGATATTTTCTGCGTCATTCCATGACGCCAGGCTTTA
CATCGCTGAGGAATATCCGA

Downstream 100 bases:

>100_bases
TCGGCCACGACCTGAGGACGCTCTGTCCTTTTGACTTGCGAATTGTCACATGACGGCGGTTGACCGGGAACGGAACCCGG
CGGCCGTCTGCCTGGCGGCT

Product: ABC-type high-affinity branched-chain amino acid transport system periplasmic binding component

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSLKLKTMTLHVVIAGCMSMAFYAQADVKIGVAGPFTGPNATYGAQYWKGASQAVADINAAGGIKGEKIVLVQGDDACEP
KQAVAVANRLVDEAKVSAVVGHFCSSSTMPASEVYDEAGILTITPGSTNPQITERGIKDLFRMCGRDDQQGAIAANYMLD
VLKAKKIAVIHDKDTYGQGLADATRAALAKRGTKEVLYEGLSRGEKDFNALVTKIGALKPDVVYFGGCHPEAGPLVRQMR
EQGVQAKFFSGDCIVTEELVTAAGGPQFTNGVLMTFGQDPRTLPDGKAVIEKFRASGFEPEGYTLYAYASIQAIAAAWNA
VGTDNAKASDWLKSHDVETVMGKKAWDGKGDLKVSDYVVYQWDDKGKYHQL

Sequences:

>Translated_371_residues
MSLKLKTMTLHVVIAGCMSMAFYAQADVKIGVAGPFTGPNATYGAQYWKGASQAVADINAAGGIKGEKIVLVQGDDACEP
KQAVAVANRLVDEAKVSAVVGHFCSSSTMPASEVYDEAGILTITPGSTNPQITERGIKDLFRMCGRDDQQGAIAANYMLD
VLKAKKIAVIHDKDTYGQGLADATRAALAKRGTKEVLYEGLSRGEKDFNALVTKIGALKPDVVYFGGCHPEAGPLVRQMR
EQGVQAKFFSGDCIVTEELVTAAGGPQFTNGVLMTFGQDPRTLPDGKAVIEKFRASGFEPEGYTLYAYASIQAIAAAWNA
VGTDNAKASDWLKSHDVETVMGKKAWDGKGDLKVSDYVVYQWDDKGKYHQL
>Mature_370_residues
SLKLKTMTLHVVIAGCMSMAFYAQADVKIGVAGPFTGPNATYGAQYWKGASQAVADINAAGGIKGEKIVLVQGDDACEPK
QAVAVANRLVDEAKVSAVVGHFCSSSTMPASEVYDEAGILTITPGSTNPQITERGIKDLFRMCGRDDQQGAIAANYMLDV
LKAKKIAVIHDKDTYGQGLADATRAALAKRGTKEVLYEGLSRGEKDFNALVTKIGALKPDVVYFGGCHPEAGPLVRQMRE
QGVQAKFFSGDCIVTEELVTAAGGPQFTNGVLMTFGQDPRTLPDGKAVIEKFRASGFEPEGYTLYAYASIQAIAAAWNAV
GTDNAKASDWLKSHDVETVMGKKAWDGKGDLKVSDYVVYQWDDKGKYHQL

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=358, Percent_Identity=42.1787709497207, Blast_Score=292, Evalue=3e-80,
Organism=Escherichia coli, GI1789867, Length=359, Percent_Identity=40.9470752089136, Blast_Score=279, Evalue=2e-76,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39696; Mature: 39564

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKLKTMTLHVVIAGCMSMAFYAQADVKIGVAGPFTGPNATYGAQYWKGASQAVADINA
CCEEEEHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHH
AGGIKGEKIVLVQGDDACEPKQAVAVANRLVDEAKVSAVVGHFCSSSTMPASEVYDEAGI
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCE
LTITPGSTNPQITERGIKDLFRMCGRDDQQGAIAANYMLDVLKAKKIAVIHDKDTYGQGL
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCH
ADATRAALAKRGTKEVLYEGLSRGEKDFNALVTKIGALKPDVVYFGGCHPEAGPLVRQMR
HHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHH
EQGVQAKFFSGDCIVTEELVTAAGGPQFTNGVLMTFGQDPRTLPDGKAVIEKFRASGFEP
HCCCCEEEECCCEEEHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCC
EGYTLYAYASIQAIAAAWNAVGTDNAKASDWLKSHDVETVMGKKAWDGKGDLKVSDYVVY
CCEEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCEEEEEEEEE
QWDDKGKYHQL
EECCCCCCCCC
>Mature Secondary Structure 
SLKLKTMTLHVVIAGCMSMAFYAQADVKIGVAGPFTGPNATYGAQYWKGASQAVADINA
CEEEEHHHHHHHHHHHHHHHHHEECCEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHH
AGGIKGEKIVLVQGDDACEPKQAVAVANRLVDEAKVSAVVGHFCSSSTMPASEVYDEAGI
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCE
LTITPGSTNPQITERGIKDLFRMCGRDDQQGAIAANYMLDVLKAKKIAVIHDKDTYGQGL
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCH
ADATRAALAKRGTKEVLYEGLSRGEKDFNALVTKIGALKPDVVYFGGCHPEAGPLVRQMR
HHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHH
EQGVQAKFFSGDCIVTEELVTAAGGPQFTNGVLMTFGQDPRTLPDGKAVIEKFRASGFEP
HCCCCEEEECCCEEEHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCCC
EGYTLYAYASIQAIAAAWNAVGTDNAKASDWLKSHDVETVMGKKAWDGKGDLKVSDYVVY
CCEEEEEEHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCEEEEEEEEE
QWDDKGKYHQL
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA