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The map label for this gene is mhpE [H]

Identifier: 238895166

GI number: 238895166

Start: 3047458

End: 3048474

Strand: Reverse

Name: mhpE [H]

Synonym: KP1_3212

Alternate gene names: 238895166

Gene position: 3048474-3047458 (Counterclockwise)

Preceding gene: 238895167

Following gene: 238895165

Centisome position: 58.08

GC content: 63.23

Gene sequence:

>1017_bases
ATGAACGGTAAAAAACTTTATATCTCGGACGTGACGCTGCGCGATGGTATGCACGCTATTCGTCACCAGTATTCCCTGGC
GCAGGTGCAGCAGATCGCCAGCGCGCTGGATAAGGCTGGGGTCGACTCCATCGAAGTGGCCCATGGCGATGGCCTGCAGG
GATCCAGCTTCAACTATGGCTTTGGCGCCCACAGCGACATCGCGTGGATTGAAGCGGCAGCGGATGTCGTGAGCCAGGCG
AAAATCGCCACCTTACTGCTGCCGGGCATCGGCACGCTGCACGATCTGAAAGCGGCGTATCAGGCCGGGGCGCGGGTGGT
GCGGGTCGCCACCCACTGCAGCGAAGCGGACGTCGCCGCGCAGCATATTGCCTTTGCCCGCGAGCTGGGGATGGACACCG
TCGGTTTTCTGATGATGAGCCATATGATCTCCCCGCAGGAGCTGGCGCAGCAGGCGCTGAAGATGGAATCTTATGGCGCG
ACCTGTATCTATGTGGTGGATTCCGGCGGGGCGATGAACATGAACGACATTCGCGACCGCTTCCGCGCCCTGAAGGCGGT
GCTGAAGCCGGAGACCGCCACCGGGATGCATGCCCATCATAATCTGAGCCTCGGGGTGGCGAACTCGATCGTCGCGGTGG
AAGAGGGGTGCGATCGCATTGACGCCAGCCTGGCCGGGATGGGGGCGGGGGCGGGCAATGCGCCGCTGGAGGTGTTTATT
GCCGCCGCCGACAAGCTCGGCTGGCAGCATGGCACCGATCTCTACGCCCTGATGAACGCGGCCGATGAGCTGGTGCGGCC
GCTGCAGGATCGCCCGGTGCGTGTCGACCGCGAAACGCTGGCGCTGGGCTACGCCGGAGTCTACTCCAGCTTCCTGCGCC
ACAGCGAAGCGGCGGCCAAGCGCTATGGCCTCAGCGCGGTGGATATTCTCGTCGAGCTGGGCAAGCGGCGGATGGTCGGC
GGCCAGGAGGATATGATCGTCGACGTCGCGCTGGATCTGCTTAACCGCAATAAATAA

Upstream 100 bases:

>100_bases
TGCCAGCGTACGCGGGCAACCTCGATATTATGACCTCGGCGGCGCTGGCGACGGCGGAGCAAATGGCCGGGGCAATGCAC
AGCGCGGCGGGAGCGACAGC

Downstream 100 bases:

>100_bases
CTAAGAACGTGCGGCTGACAAAGCATCAGCCGCCAGTCACCTGCTCCGTTTGTTCGGCTATCGCCAAGGTACCCTATGAC
CAAGATAACGACTGCAACCC

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MNGKKLYISDVTLRDGMHAIRHQYSLAQVQQIASALDKAGVDSIEVAHGDGLQGSSFNYGFGAHSDIAWIEAAADVVSQA
KIATLLLPGIGTLHDLKAAYQAGARVVRVATHCSEADVAAQHIAFARELGMDTVGFLMMSHMISPQELAQQALKMESYGA
TCIYVVDSGGAMNMNDIRDRFRALKAVLKPETATGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLEVFI
AAADKLGWQHGTDLYALMNAADELVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEAAAKRYGLSAVDILVELGKRRMVG
GQEDMIVDVALDLLNRNK

Sequences:

>Translated_338_residues
MNGKKLYISDVTLRDGMHAIRHQYSLAQVQQIASALDKAGVDSIEVAHGDGLQGSSFNYGFGAHSDIAWIEAAADVVSQA
KIATLLLPGIGTLHDLKAAYQAGARVVRVATHCSEADVAAQHIAFARELGMDTVGFLMMSHMISPQELAQQALKMESYGA
TCIYVVDSGGAMNMNDIRDRFRALKAVLKPETATGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLEVFI
AAADKLGWQHGTDLYALMNAADELVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEAAAKRYGLSAVDILVELGKRRMVG
GQEDMIVDVALDLLNRNK
>Mature_338_residues
MNGKKLYISDVTLRDGMHAIRHQYSLAQVQQIASALDKAGVDSIEVAHGDGLQGSSFNYGFGAHSDIAWIEAAADVVSQA
KIATLLLPGIGTLHDLKAAYQAGARVVRVATHCSEADVAAQHIAFARELGMDTVGFLMMSHMISPQELAQQALKMESYGA
TCIYVVDSGGAMNMNDIRDRFRALKAVLKPETATGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLEVFI
AAADKLGWQHGTDLYALMNAADELVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEAAAKRYGLSAVDILVELGKRRMVG
GQEDMIVDVALDLLNRNK

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1786548, Length=335, Percent_Identity=89.5522388059701, Blast_Score=592, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- ProDom:   PD005364 [H]

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 36142; Mature: 36142

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGKKLYISDVTLRDGMHAIRHQYSLAQVQQIASALDKAGVDSIEVAHGDGLQGSSFNYG
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
FGAHSDIAWIEAAADVVSQAKIATLLLPGIGTLHDLKAAYQAGARVVRVATHCSEADVAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHEEHHHHHCCHHHHHH
QHIAFARELGMDTVGFLMMSHMISPQELAQQALKMESYGATCIYVVDSGGAMNMNDIRDR
HHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHH
FRALKAVLKPETATGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLEVFI
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
AAADKLGWQHGTDLYALMNAADELVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEAAAK
EEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
RYGLSAVDILVELGKRRMVGGQEDMIVDVALDLLNRNK
HHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNGKKLYISDVTLRDGMHAIRHQYSLAQVQQIASALDKAGVDSIEVAHGDGLQGSSFNYG
CCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
FGAHSDIAWIEAAADVVSQAKIATLLLPGIGTLHDLKAAYQAGARVVRVATHCSEADVAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHEEHHHHHCCHHHHHH
QHIAFARELGMDTVGFLMMSHMISPQELAQQALKMESYGATCIYVVDSGGAMNMNDIRDR
HHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHH
FRALKAVLKPETATGMHAHHNLSLGVANSIVAVEEGCDRIDASLAGMGAGAGNAPLEVFI
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
AAADKLGWQHGTDLYALMNAADELVRPLQDRPVRVDRETLALGYAGVYSSFLRHSEAAAK
EEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHH
RYGLSAVDILVELGKRRMVGGQEDMIVDVALDLLNRNK
HHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA