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The map label for this gene is emrB [H]

Identifier: 238894812

GI number: 238894812

Start: 2693091

End: 2694653

Strand: Direct

Name: emrB [H]

Synonym: KP1_2825

Alternate gene names: 238894812

Gene position: 2693091-2694653 (Clockwise)

Preceding gene: 238894811

Following gene: 238894813

Centisome position: 51.31

GC content: 62.25

Gene sequence:

>1563_bases
ATGAGCACCGTTCAGGCGCTGTCCGCCCCGCAGGGTCTGTCGATGCCGACGGCGAAGAAGATCTTCGCCTTCGCCAGCAT
GTGCGTGGGGATATTTATTGCCCTTATCGACATCCAGATCGTCTCCGCCTCGCTGCGGGATATTGGCGGCGGCCTGTCGG
CGGGCGACGATGAAACCGTCTGGGTACAAACCAGCTACCTGATCGCCGAGATCATTATTATCCCGCTTTCCGGCTGGCTG
GCCCGGGTCATGTCGACCCGCTGGCTGTTTGCCGCTTCCGCGGCGGGCTTCACGCTGATGAGCCTGCTGTGCGGCTGGGC
GTGGAACATCCAGAGTATGATCGCCTTCCGCGCCCTGCAGGGACTGGCGGGCGGGTCGATGATCCCCCTGGTGTTCACCA
CCGCTTTCGCCTTTTTTCAGGGCAAACAGCGGGTCATCGCCGCGGCGACGATCGGCGGCCTGGCCTCCCTCGCCCCTACG
CTGGGTCCAACGGTGGGCGGCTGGATCACCGAAAACTACAACTGGCACTGGCTGTTCTTTATCAACGTTGTTCCGGGGAT
TTACATCGCGGTGGCAGTGCCGCTGCTGGTAAAAGTAGACAGCGCCGCCCCCACTCTGCTGCGCGGCGCCGACTATCTCA
GTATTCTGCTGCTGGCCTTGTCCCTCGGCTGCCTGGAATATACCCTCGAAGAAGGTCCGCGCTGGGGCTGGTTTGATGAC
GCGACCCTGACGACCACCGCCTGGGTCGCCCTGCTCTGCGGCGTGGCCTTTGTAATCCGCACCCTGCGCCATCCGCAGCC
GGTGATGGATCTCCGCGCCCTGCAGGATCGAACCTTCAGCCTCGGCTGCTATTTCTCGTTTATGGCCGGCGTCGGCATCT
TCGCCACCATCTATTTAACCCCGCTGTATCTGGGGAGCGTGCGCGGTTTCAGTGCGCTGGAGATCGGCCTGGCGGTATTT
TCCACCGGTCTGTTCCAGGTCATGTCTATTCCGTTTTATTCGTGGCTCGCCAACCGCGTCGATCTGCGCTGGCTGCTGAT
GGCCGGGCTGATCGGCTTTGCGGTGTCAATGTACAGCTTTGTTCCGATCACTCACGACTGGGGGGCGGACCAGTTGCTGC
TCCCGCAGGCCTTTCGCGGACTGGCGCAGCAGTTTGCCGTCGCGCCAACGGTGACCCTGACGCTGGGCAGCCTGCCGCCC
GCGCGCCTGAAGCTGGCCTCCGGGCTGTTTAATCTGATGCGCAACCTCGGGGGCGCCATCGGCATCGCCCTGTGCGGCAC
GGTGCTTAACGACAGAACCAATCTGCACTATAGCCGCCTGGCGGACCATCTGAATAACGCCAACCTGGCAATGAGCGATT
TTGTTCAGCGCAGCGCGGCAAACTTCACCGTCCAGGGGATCTCGCCGGATGCCGCGCAGACGGCGGCGCTGAAAAATCTT
TCCGCGCTGGCGCTGCGGGAGGCGCGAACCCAGGCCTTTTCCGATGCGTTCTACCTCATCATGATGGGTTTTTTACTTGC
CGCGCTGCTGGTTCCCTTGATGAAAAAGCCGCCGGCACACTGA

Upstream 100 bases:

>100_bases
GGGTGCCGGTCCGCATTGCGCTGGAAGGTGACGGGGCAAAGCTTGACGTCCTGCGCCCGGGACTGTCGGTGATCGTCACC
GTGAACGAAAAGAGCCCTCG

Downstream 100 bases:

>100_bases
TACTACAGGAGAGTTAACTATGGGCGTTTCCGTTAAACGCATTGTCGTCACCGGGATGGGGATTGTCAGCCCGCTGGGCT
GCGGCGTACAGCACGTCTGG

Product: multidrug resistance MFS membrane translocase

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSTVQALSAPQGLSMPTAKKIFAFASMCVGIFIALIDIQIVSASLRDIGGGLSAGDDETVWVQTSYLIAEIIIIPLSGWL
ARVMSTRWLFAASAAGFTLMSLLCGWAWNIQSMIAFRALQGLAGGSMIPLVFTTAFAFFQGKQRVIAAATIGGLASLAPT
LGPTVGGWITENYNWHWLFFINVVPGIYIAVAVPLLVKVDSAAPTLLRGADYLSILLLALSLGCLEYTLEEGPRWGWFDD
ATLTTTAWVALLCGVAFVIRTLRHPQPVMDLRALQDRTFSLGCYFSFMAGVGIFATIYLTPLYLGSVRGFSALEIGLAVF
STGLFQVMSIPFYSWLANRVDLRWLLMAGLIGFAVSMYSFVPITHDWGADQLLLPQAFRGLAQQFAVAPTVTLTLGSLPP
ARLKLASGLFNLMRNLGGAIGIALCGTVLNDRTNLHYSRLADHLNNANLAMSDFVQRSAANFTVQGISPDAAQTAALKNL
SALALREARTQAFSDAFYLIMMGFLLAALLVPLMKKPPAH

Sequences:

>Translated_520_residues
MSTVQALSAPQGLSMPTAKKIFAFASMCVGIFIALIDIQIVSASLRDIGGGLSAGDDETVWVQTSYLIAEIIIIPLSGWL
ARVMSTRWLFAASAAGFTLMSLLCGWAWNIQSMIAFRALQGLAGGSMIPLVFTTAFAFFQGKQRVIAAATIGGLASLAPT
LGPTVGGWITENYNWHWLFFINVVPGIYIAVAVPLLVKVDSAAPTLLRGADYLSILLLALSLGCLEYTLEEGPRWGWFDD
ATLTTTAWVALLCGVAFVIRTLRHPQPVMDLRALQDRTFSLGCYFSFMAGVGIFATIYLTPLYLGSVRGFSALEIGLAVF
STGLFQVMSIPFYSWLANRVDLRWLLMAGLIGFAVSMYSFVPITHDWGADQLLLPQAFRGLAQQFAVAPTVTLTLGSLPP
ARLKLASGLFNLMRNLGGAIGIALCGTVLNDRTNLHYSRLADHLNNANLAMSDFVQRSAANFTVQGISPDAAQTAALKNL
SALALREARTQAFSDAFYLIMMGFLLAALLVPLMKKPPAH
>Mature_519_residues
STVQALSAPQGLSMPTAKKIFAFASMCVGIFIALIDIQIVSASLRDIGGGLSAGDDETVWVQTSYLIAEIIIIPLSGWLA
RVMSTRWLFAASAAGFTLMSLLCGWAWNIQSMIAFRALQGLAGGSMIPLVFTTAFAFFQGKQRVIAAATIGGLASLAPTL
GPTVGGWITENYNWHWLFFINVVPGIYIAVAVPLLVKVDSAAPTLLRGADYLSILLLALSLGCLEYTLEEGPRWGWFDDA
TLTTTAWVALLCGVAFVIRTLRHPQPVMDLRALQDRTFSLGCYFSFMAGVGIFATIYLTPLYLGSVRGFSALEIGLAVFS
TGLFQVMSIPFYSWLANRVDLRWLLMAGLIGFAVSMYSFVPITHDWGADQLLLPQAFRGLAQQFAVAPTVTLTLGSLPPA
RLKLASGLFNLMRNLGGAIGIALCGTVLNDRTNLHYSRLADHLNNANLAMSDFVQRSAANFTVQGISPDAAQTAALKNLS
ALALREARTQAFSDAFYLIMMGFLLAALLVPLMKKPPAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=435, Percent_Identity=31.264367816092, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1788710, Length=501, Percent_Identity=27.3453093812375, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1790195, Length=441, Percent_Identity=24.2630385487528, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1788392, Length=411, Percent_Identity=26.7639902676399, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87081983, Length=432, Percent_Identity=26.1574074074074, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56058; Mature: 55926

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVQALSAPQGLSMPTAKKIFAFASMCVGIFIALIDIQIVSASLRDIGGGLSAGDDETV
CCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
WVQTSYLIAEIIIIPLSGWLARVMSTRWLFAASAAGFTLMSLLCGWAWNIQSMIAFRALQ
EEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAGGSMIPLVFTTAFAFFQGKQRVIAAATIGGLASLAPTLGPTVGGWITENYNWHWLFF
HCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEE
INVVPGIYIAVAVPLLVKVDSAAPTLLRGADYLSILLLALSLGCLEYTLEEGPRWGWFDD
EHHHHHHHHHHHHHHHHEECCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ATLTTTAWVALLCGVAFVIRTLRHPQPVMDLRALQDRTFSLGCYFSFMAGVGIFATIYLT
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHH
PLYLGSVRGFSALEIGLAVFSTGLFQVMSIPFYSWLANRVDLRWLLMAGLIGFAVSMYSF
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPITHDWGADQLLLPQAFRGLAQQFAVAPTVTLTLGSLPPARLKLASGLFNLMRNLGGAI
CCEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCHHH
GIALCGTVLNDRTNLHYSRLADHLNNANLAMSDFVQRSAANFTVQGISPDAAQTAALKNL
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
SALALREARTQAFSDAFYLIMMGFLLAALLVPLMKKPPAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
STVQALSAPQGLSMPTAKKIFAFASMCVGIFIALIDIQIVSASLRDIGGGLSAGDDETV
CCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
WVQTSYLIAEIIIIPLSGWLARVMSTRWLFAASAAGFTLMSLLCGWAWNIQSMIAFRALQ
EEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GLAGGSMIPLVFTTAFAFFQGKQRVIAAATIGGLASLAPTLGPTVGGWITENYNWHWLFF
HCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEE
INVVPGIYIAVAVPLLVKVDSAAPTLLRGADYLSILLLALSLGCLEYTLEEGPRWGWFDD
EHHHHHHHHHHHHHHHHEECCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
ATLTTTAWVALLCGVAFVIRTLRHPQPVMDLRALQDRTFSLGCYFSFMAGVGIFATIYLT
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHH
PLYLGSVRGFSALEIGLAVFSTGLFQVMSIPFYSWLANRVDLRWLLMAGLIGFAVSMYSF
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VPITHDWGADQLLLPQAFRGLAQQFAVAPTVTLTLGSLPPARLKLASGLFNLMRNLGGAI
CCEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCHHH
GIALCGTVLNDRTNLHYSRLADHLNNANLAMSDFVQRSAANFTVQGISPDAAQTAALKNL
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
SALALREARTQAFSDAFYLIMMGFLLAALLVPLMKKPPAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]