Click here to switch to the map view.

The map label for this gene is ynfJ [H]

Identifier: 238894598

GI number: 238894598

Start: 2493307

End: 2494602

Strand: Reverse

Name: ynfJ [H]

Synonym: KP1_2599

Alternate gene names: 238894598

Gene position: 2494602-2493307 (Counterclockwise)

Preceding gene: 238894599

Following gene: 238894594

Centisome position: 47.53

GC content: 63.04

Gene sequence:

>1296_bases
ATGCATCGTCTTCATCCCTATCCTGATGTGCAAGTGATGTTTCGCCGCCTGCTGATCGCCACCCTGATTGGCCTTCTCGC
CGCCCTGGCGGTGGCGCTGTTTCGCCACGCTATGGTGGTGCTGGAAACGCTGTTGCTCAGTAATGACAGCGGGAGCCTGG
TCAACGCCGCACAGTCGCTTCCGGCGTGGCGTCGGCTGGTTACCCCCGCGCTGGGCGGCCTGACGGCGGGAACCTTACTC
TGGCTCTGGCAACGACGCAGCGTCGCGCGTCCGCATGCCGCCACCGATTATATGGAAGCCCTGGAGACCGGCGACGGCTG
TTTCGATACGCCGGCAAGCCTGGTGAAATCGCTGGCGTCATTGCTGGTCGTGGTGACCGGCAGCGCGATTGGCCGGGAAG
GCGCCATGATCCTGCTGGCCGCGCTGGCGGCCTCGCTGTTTGCCCGTCGCTTCACGCCGCAGAGTGAATGGAAGCTATGG
GTCGCCTGCGGCGCGGCTGCCGGGATGGCCAGCGCCTATCATGCCCCGCTGGCCGGCAGCCTGTTTATTGCTGAAATTCT
CTTTGGCACGCTGATGCTGGCCTCGCTTGGGCCGGTGGTGATCTCGGCGGTGATCGCCCTGCTGTTGACGCAGTTTCTTA
ATGGCGGCGCCGCGCCGCTGTATCACGTCGTGTTACAGCAAAACCTCAGCGCCCTGCACTATGGCTTAATGCTGGCGACT
GGCCTGCTGGCCGGGCTGTGCGGGCCGCTGTTTATCTGGCTGATGGACTACAGCCACCGTGGGTTCGTTAAACTAAAGCT
GGCGCCGCCGTGGCAGCTGGCGCTGGGCGGGCTAATTGTCGGCGGCTTATCGCTCATCACCCCGGCAGTATGGGGCAACG
GCTACAGCGTAGTGCAAAGCTATTTGCTGCTGCCGCCGTCCGGTGCGCTGCTGGTGGGCATATTTATCTGCAAACTGCTG
GCGGTGCTGGCCAGCAGCGGCTCCGGCGCGCCTGGCGGGGTCTTCACCCCGACGCTGTTTGTCGGCCTGGCGATGGGCAT
GCTGTTTGCCTGCTTCAGCAGGCTGTGGCTACCGGGAAGTGAAGAAATGGCCATCATGATGGGTCTGACCGGCATGGCGG
CGTTTCTGGCCGCCACGACCCATGCGCCGATCATGTCAACCCTGATGATTTGCGAGATGACCGGGCAGTATACGCTGCTG
CCCGGTCTGCTGATAACCTGCGTCGTGTCGTCTGTGCTGTCGCGGACGTTACGCCGGGATTCGATCTATCGCCATCACGT
TGCCGAGCATGTCTAA

Upstream 100 bases:

>100_bases
TTTTGCCGCTTCCCAGCCTTTCACCCGCACTTATCGCTTTTGCATCTAAGGTTATTCCCTATCACAATAAGCCAATGTTT
TCTGAACCTCGCCGTGACTA

Downstream 100 bases:

>100_bases
GTCGACGTAGCGGCTCAGCTCACGCTGTTCGGCGCGCGGTAGATAAGGCAGCTCGCCGATCAGCGGCGCCGGCAGCTTTT
TGCTGAGGACATCGATGATT

Product: putative voltage-gated ClC-type chloride channel ClcB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MHRLHPYPDVQVMFRRLLIATLIGLLAALAVALFRHAMVVLETLLLSNDSGSLVNAAQSLPAWRRLVTPALGGLTAGTLL
WLWQRRSVARPHAATDYMEALETGDGCFDTPASLVKSLASLLVVVTGSAIGREGAMILLAALAASLFARRFTPQSEWKLW
VACGAAAGMASAYHAPLAGSLFIAEILFGTLMLASLGPVVISAVIALLLTQFLNGGAAPLYHVVLQQNLSALHYGLMLAT
GLLAGLCGPLFIWLMDYSHRGFVKLKLAPPWQLALGGLIVGGLSLITPAVWGNGYSVVQSYLLLPPSGALLVGIFICKLL
AVLASSGSGAPGGVFTPTLFVGLAMGMLFACFSRLWLPGSEEMAIMMGLTGMAAFLAATTHAPIMSTLMICEMTGQYTLL
PGLLITCVVSSVLSRTLRRDSIYRHHVAEHV

Sequences:

>Translated_431_residues
MHRLHPYPDVQVMFRRLLIATLIGLLAALAVALFRHAMVVLETLLLSNDSGSLVNAAQSLPAWRRLVTPALGGLTAGTLL
WLWQRRSVARPHAATDYMEALETGDGCFDTPASLVKSLASLLVVVTGSAIGREGAMILLAALAASLFARRFTPQSEWKLW
VACGAAAGMASAYHAPLAGSLFIAEILFGTLMLASLGPVVISAVIALLLTQFLNGGAAPLYHVVLQQNLSALHYGLMLAT
GLLAGLCGPLFIWLMDYSHRGFVKLKLAPPWQLALGGLIVGGLSLITPAVWGNGYSVVQSYLLLPPSGALLVGIFICKLL
AVLASSGSGAPGGVFTPTLFVGLAMGMLFACFSRLWLPGSEEMAIMMGLTGMAAFLAATTHAPIMSTLMICEMTGQYTLL
PGLLITCVVSSVLSRTLRRDSIYRHHVAEHV
>Mature_431_residues
MHRLHPYPDVQVMFRRLLIATLIGLLAALAVALFRHAMVVLETLLLSNDSGSLVNAAQSLPAWRRLVTPALGGLTAGTLL
WLWQRRSVARPHAATDYMEALETGDGCFDTPASLVKSLASLLVVVTGSAIGREGAMILLAALAASLFARRFTPQSEWKLW
VACGAAAGMASAYHAPLAGSLFIAEILFGTLMLASLGPVVISAVIALLLTQFLNGGAAPLYHVVLQQNLSALHYGLMLAT
GLLAGLCGPLFIWLMDYSHRGFVKLKLAPPWQLALGGLIVGGLSLITPAVWGNGYSVVQSYLLLPPSGALLVGIFICKLL
AVLASSGSGAPGGVFTPTLFVGLAMGMLFACFSRLWLPGSEEMAIMMGLTGMAAFLAATTHAPIMSTLMICEMTGQYTLL
PGLLITCVVSSVLSRTLRRDSIYRHHVAEHV

Specific function: Probably acts as an electrical shunt for an outwardly- directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response [H]

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081943, Length=418, Percent_Identity=72.2488038277512, Blast_Score=530, Evalue=1e-152,
Organism=Escherichia coli, GI1786350, Length=252, Percent_Identity=30.1587301587302, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 45629; Mature: 45629

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRLHPYPDVQVMFRRLLIATLIGLLAALAVALFRHAMVVLETLLLSNDSGSLVNAAQSL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
PAWRRLVTPALGGLTAGTLLWLWQRRSVARPHAATDYMEALETGDGCFDTPASLVKSLAS
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LLVVVTGSAIGREGAMILLAALAASLFARRFTPQSEWKLWVACGAAAGMASAYHAPLAGS
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCHHH
LFIAEILFGTLMLASLGPVVISAVIALLLTQFLNGGAAPLYHVVLQQNLSALHYGLMLAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLLAGLCGPLFIWLMDYSHRGFVKLKLAPPWQLALGGLIVGGLSLITPAVWGNGYSVVQS
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YLLLPPSGALLVGIFICKLLAVLASSGSGAPGGVFTPTLFVGLAMGMLFACFSRLWLPGS
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EEMAIMMGLTGMAAFLAATTHAPIMSTLMICEMTGQYTLLPGLLITCVVSSVLSRTLRRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SIYRHHVAEHV
HHHHHHHHHCC
>Mature Secondary Structure
MHRLHPYPDVQVMFRRLLIATLIGLLAALAVALFRHAMVVLETLLLSNDSGSLVNAAQSL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
PAWRRLVTPALGGLTAGTLLWLWQRRSVARPHAATDYMEALETGDGCFDTPASLVKSLAS
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LLVVVTGSAIGREGAMILLAALAASLFARRFTPQSEWKLWVACGAAAGMASAYHAPLAGS
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCCHHH
LFIAEILFGTLMLASLGPVVISAVIALLLTQFLNGGAAPLYHVVLQQNLSALHYGLMLAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
GLLAGLCGPLFIWLMDYSHRGFVKLKLAPPWQLALGGLIVGGLSLITPAVWGNGYSVVQS
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
YLLLPPSGALLVGIFICKLLAVLASSGSGAPGGVFTPTLFVGLAMGMLFACFSRLWLPGS
HHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
EEMAIMMGLTGMAAFLAATTHAPIMSTLMICEMTGQYTLLPGLLITCVVSSVLSRTLRRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
SIYRHHVAEHV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA