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The map label for this gene is bioD [H]

Identifier: 238894597

GI number: 238894597

Start: 2492599

End: 2493354

Strand: Direct

Name: bioD [H]

Synonym: KP1_2598

Alternate gene names: 238894597

Gene position: 2492599-2493354 (Clockwise)

Preceding gene: 238894596

Following gene: 238894604

Centisome position: 47.49

GC content: 58.47

Gene sequence:

>756_bases
TTGCCCACCGTTGATTATTTCTCTCCCGTATATTTCATCGACAAAACTGTGGGGTTAGTGATGTTTAAGCGTTTCTTTAT
TACAGGTACAGACACCTCTGTCGGGAAGACAGTGGTTTCCCGTGCGCTGTTGCAGGCACTTGCTGCCAGAGGCAAAAGCG
TGGCCGGGTATAAGCCTGTGGCGAAGGGCAGCAAAGAGACGCCGGACGGTTTACGTAACAAAGATGCGCTGGTGTTGCAG
AGCGTCTCCACGCTGGCCTTACCCTATGAGGCTGTCAACCCCATCGCCTTAAGCGAAGACGAGAGCAGCGTGGCGCATAG
CTGCCCGATCAACTACGGTCTGCTGTCCGATGGTTTGCAGCGTCTGGCCGCGCAGGTCGAACACGTGGTGGTTGAAGGCA
CCGGCGGCTGGCGCAGCCTGATGAACGACCTGCGGCCGCTCTCCGAGTGGGTGGTTCAGGAGCAGCTCCCAGTGCTGATG
GTGGTGGGGATCCAGGAGGGGTGCATCAACCATGCGCTACTGACAGCCCAGGCGATCGCCAATGACGGTTTACCGCTGAT
TGGCTGGGTGGCCAATCGGATCAACCCCGGTCTGGCGCACTACGCCGAAATCATCGATGTCCTCAGCAAAAAGCTGCCGG
CGCCGCTGATCGGCGAGCTGCCTTATCTACCGCGCGCCGAACAGCGTGAGCTGAGCCGCTACGTCGACTTAGACATGCTC
GGCAACGTGATGGCGATAGATCGAATCCCGGCGTAA

Upstream 100 bases:

>100_bases
GGATCCTTACTCATACGACTGTTACAGGGTTAACATTTTTTCATAGTCACACCAAAAATTGCGCTATCTCAAGCTGACCT
CAGACGGCTTCACGTAGACT

Downstream 100 bases:

>100_bases
CGTCCGCGACAGCACAGACGACACGACGCAGGTTATCAGCAGACCGGGCAGCAGCGTATACTGCCCGGTCATCTCGCAAA
TCATCAGGGTTGACATGATC

Product: putative dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MPTVDYFSPVYFIDKTVGLVMFKRFFITGTDTSVGKTVVSRALLQALAARGKSVAGYKPVAKGSKETPDGLRNKDALVLQ
SVSTLALPYEAVNPIALSEDESSVAHSCPINYGLLSDGLQRLAAQVEHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLM
VVGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAHYAEIIDVLSKKLPAPLIGELPYLPRAEQRELSRYVDLDML
GNVMAIDRIPA

Sequences:

>Translated_251_residues
MPTVDYFSPVYFIDKTVGLVMFKRFFITGTDTSVGKTVVSRALLQALAARGKSVAGYKPVAKGSKETPDGLRNKDALVLQ
SVSTLALPYEAVNPIALSEDESSVAHSCPINYGLLSDGLQRLAAQVEHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLM
VVGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAHYAEIIDVLSKKLPAPLIGELPYLPRAEQRELSRYVDLDML
GNVMAIDRIPA
>Mature_250_residues
PTVDYFSPVYFIDKTVGLVMFKRFFITGTDTSVGKTVVSRALLQALAARGKSVAGYKPVAKGSKETPDGLRNKDALVLQS
VSTLALPYEAVNPIALSEDESSVAHSCPINYGLLSDGLQRLAAQVEHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMV
VGIQEGCINHALLTAQAIANDGLPLIGWVANRINPGLAHYAEIIDVLSKKLPAPLIGELPYLPRAEQRELSRYVDLDMLG
NVMAIDRIPA

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI87081944, Length=229, Percent_Identity=87.7729257641921, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1786995, Length=225, Percent_Identity=50.2222222222222, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 27202; Mature: 27071

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTVDYFSPVYFIDKTVGLVMFKRFFITGTDTSVGKTVVSRALLQALAARGKSVAGYKPV
CCCCCHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AKGSKETPDGLRNKDALVLQSVSTLALPYEAVNPIALSEDESSVAHSCPINYGLLSDGLQ
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCEECCCCHHHHHHCCCCCHHHHHHHH
RLAAQVEHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
HHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHH
NDGLPLIGWVANRINPGLAHYAEIIDVLSKKLPAPLIGELPYLPRAEQRELSRYVDLDML
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHCHHHH
GNVMAIDRIPA
CCCHHHCCCCC
>Mature Secondary Structure 
PTVDYFSPVYFIDKTVGLVMFKRFFITGTDTSVGKTVVSRALLQALAARGKSVAGYKPV
CCCCHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AKGSKETPDGLRNKDALVLQSVSTLALPYEAVNPIALSEDESSVAHSCPINYGLLSDGLQ
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCCEECCCCHHHHHHCCCCCHHHHHHHH
RLAAQVEHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
HHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHH
NDGLPLIGWVANRINPGLAHYAEIIDVLSKKLPAPLIGELPYLPRAEQRELSRYVDLDML
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHCHHHH
GNVMAIDRIPA
CCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]