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The map label for this gene is oprM [H]

Identifier: 238894498

GI number: 238894498

Start: 2394059

End: 2395438

Strand: Reverse

Name: oprM [H]

Synonym: KP1_2489

Alternate gene names: 238894498

Gene position: 2395438-2394059 (Counterclockwise)

Preceding gene: 238894499

Following gene: 238894497

Centisome position: 45.64

GC content: 63.77

Gene sequence:

>1380_bases
ATGATCCGCCCGGTCGCCCTTGCTATCGTTCTGGCGCTGGTCGGCTGTCAATCCGCCGATGTGCAGCGCGCACAGCCGAC
CCTGACCATTCCTGCCGCCTGGCGGGCCGACGTTGGCCCGGCCAGCCCGGTGGAGGGCGTATGGTGGCGCAATTTTCATG
ACAGTACCCTCAATCAGTATGTCGACCAGGCGCTGCGCTATAACAGCGATGTGCTGATCGCCCGCGAGCGGGTGAATGAG
TATCAGGCGCGCGCTTACGCCGCCGACAGCAGCCTGTTTCCGTCACTGGACGCCAGCCTCACCGGCACCCGCGCCCGGAC
GCAGTCCGCCGCCACCGGCCTGCCGATCCACAGCACACTGTATAAGGGCGGCCTGACCGCCAGCTATGATGTCGATATCT
GGGGAGCGAACCGCAGCGCGGCCAACGCCGCCGGGGCCAGCCTTGAGGCGCAGAAAGCCGCCGCCGCCGCCGCCAATTTG
AGCGTCGCCAGCTCGGTGGCCGTTGGCTACGTTACGCTGCTGTCGCTGGATGAGCAGCTGCGGGTGACCCAGCAGACGCT
AACATCAAGGGAAGACGCGTGGCGCCTGGCCAAACGTCAGTTTGAAACCGGGTATACTTCACGCCTTGAGCTGATGCAGG
CGGATTCGGAGCTGCGGTCAACGCGGGCGCAGATCCCGCCGCTACAGCACCAGATTGCGCAGCAGGAAAATGCGCTCAGC
GTGCTGCTCGGCGATAACCCTGGCGCGGTAAAACGCGGCGAGTTCGCCCAGCTTACCCCGCTGCGTCTGCCGTCGCAGCT
GCCGTCCACCTTGCTCAACCGCCGTCCGGACATCGCTCAGGCCGAGCGCCAGCTGGTGGCCGCCGACGCCACGCTGGCCT
CGTCGCAGGCGCAGCTGCTGCCCTCTATTAACCTGACCGCTACCGGCAGCCTGCAGGACCGGACGCTGCCGGATCTGCTG
GACAACCCGCTGCGGCTGTGGAGTCTTGGCGGCAGTATTCTGGCGCCGCTGCTAAACCGCCAGGCGTTAAATGCCCAGGT
GGACGTCTCCATGGCCCAGCGCAACCAGGCGCTCTATAGCTATGAGAAAACGGTGCGCAGCGCGTTCAAAGAGGTCAATG
ACAGCCTGGACGCCATCAGTCGCTATGGCGAACAGTTAACCGAGCTTCAGGAACAAGAGACCGTTGCCCAGGAAACCTTA
CGTATTGCACAAAACCGCTACCGTAACGGCTACTCTTCTTATCTGGATGTGCTTGATGCCCAGCGAACGCTGTTCTCCAC
CCAGCTTAGCGTGGTGCAGGTGAAGAACAACCTGCTGCTGGCGCAGATCGCTCTGTACCGGGCGCTGGGAGGCGGCTGGT
CTGACTCATCAGGGAGCTAA

Upstream 100 bases:

>100_bases
CGGTGCGTATTACGGTCAACGACGGGCAAAACAACAGCGAACGCCTGCGGCCGGGGATGTCGGTGCAGGTGACTATTGAT
ACCCGTGCGGAGAAACAACC

Downstream 100 bases:

>100_bases
ATGGGGAAGCGTCATATGCAACAACAGTGGTCGGCAGTAGACAATTATTTGATTAAGGCCTTAATTCCCGGCGATCCTGT
GCTCGATCGGGTACTGGAAA

Product: putative outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MIRPVALAIVLALVGCQSADVQRAQPTLTIPAAWRADVGPASPVEGVWWRNFHDSTLNQYVDQALRYNSDVLIARERVNE
YQARAYAADSSLFPSLDASLTGTRARTQSAATGLPIHSTLYKGGLTASYDVDIWGANRSAANAAGASLEAQKAAAAAANL
SVASSVAVGYVTLLSLDEQLRVTQQTLTSREDAWRLAKRQFETGYTSRLELMQADSELRSTRAQIPPLQHQIAQQENALS
VLLGDNPGAVKRGEFAQLTPLRLPSQLPSTLLNRRPDIAQAERQLVAADATLASSQAQLLPSINLTATGSLQDRTLPDLL
DNPLRLWSLGGSILAPLLNRQALNAQVDVSMAQRNQALYSYEKTVRSAFKEVNDSLDAISRYGEQLTELQEQETVAQETL
RIAQNRYRNGYSSYLDVLDAQRTLFSTQLSVVQVKNNLLLAQIALYRALGGGWSDSSGS

Sequences:

>Translated_459_residues
MIRPVALAIVLALVGCQSADVQRAQPTLTIPAAWRADVGPASPVEGVWWRNFHDSTLNQYVDQALRYNSDVLIARERVNE
YQARAYAADSSLFPSLDASLTGTRARTQSAATGLPIHSTLYKGGLTASYDVDIWGANRSAANAAGASLEAQKAAAAAANL
SVASSVAVGYVTLLSLDEQLRVTQQTLTSREDAWRLAKRQFETGYTSRLELMQADSELRSTRAQIPPLQHQIAQQENALS
VLLGDNPGAVKRGEFAQLTPLRLPSQLPSTLLNRRPDIAQAERQLVAADATLASSQAQLLPSINLTATGSLQDRTLPDLL
DNPLRLWSLGGSILAPLLNRQALNAQVDVSMAQRNQALYSYEKTVRSAFKEVNDSLDAISRYGEQLTELQEQETVAQETL
RIAQNRYRNGYSSYLDVLDAQRTLFSTQLSVVQVKNNLLLAQIALYRALGGGWSDSSGS
>Mature_459_residues
MIRPVALAIVLALVGCQSADVQRAQPTLTIPAAWRADVGPASPVEGVWWRNFHDSTLNQYVDQALRYNSDVLIARERVNE
YQARAYAADSSLFPSLDASLTGTRARTQSAATGLPIHSTLYKGGLTASYDVDIWGANRSAANAAGASLEAQKAAAAAANL
SVASSVAVGYVTLLSLDEQLRVTQQTLTSREDAWRLAKRQFETGYTSRLELMQADSELRSTRAQIPPLQHQIAQQENALS
VLLGDNPGAVKRGEFAQLTPLRLPSQLPSTLLNRRPDIAQAERQLVAADATLASSQAQLLPSINLTATGSLQDRTLPDLL
DNPLRLWSLGGSILAPLLNRQALNAQVDVSMAQRNQALYSYEKTVRSAFKEVNDSLDAISRYGEQLTELQEQETVAQETL
RIAQNRYRNGYSSYLDVLDAQRTLFSTQLSVVQVKNNLLLAQIALYRALGGGWSDSSGS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=455, Percent_Identity=30.3296703296703, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI2367348, Length=428, Percent_Identity=24.7663551401869, Blast_Score=97, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49922; Mature: 49922

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPVALAIVLALVGCQSADVQRAQPTLTIPAAWRADVGPASPVEGVWWRNFHDSTLNQY
CCCHHHHHHHHHHHCCCCCCCHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VDQALRYNSDVLIARERVNEYQARAYAADSSLFPSLDASLTGTRARTQSAATGLPIHSTL
HHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHH
YKGGLTASYDVDIWGANRSAANAAGASLEAQKAAAAAANLSVASSVAVGYVTLLSLDEQL
HCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVTQQTLTSREDAWRLAKRQFETGYTSRLELMQADSELRSTRAQIPPLQHQIAQQENALS
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEE
VLLGDNPGAVKRGEFAQLTPLRLPSQLPSTLLNRRPDIAQAERQLVAADATLASSQAQLL
EEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHC
PSINLTATGSLQDRTLPDLLDNPLRLWSLGGSILAPLLNRQALNAQVDVSMAQRNQALYS
CCCCEEECCCCCCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YEKTVRSAFKEVNDSLDAISRYGEQLTELQEQETVAQETLRIAQNRYRNGYSSYLDVLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QRTLFSTQLSVVQVKNNLLLAQIALYRALGGGWSDSSGS
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIRPVALAIVLALVGCQSADVQRAQPTLTIPAAWRADVGPASPVEGVWWRNFHDSTLNQY
CCCHHHHHHHHHHHCCCCCCCHHCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
VDQALRYNSDVLIARERVNEYQARAYAADSSLFPSLDASLTGTRARTQSAATGLPIHSTL
HHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHH
YKGGLTASYDVDIWGANRSAANAAGASLEAQKAAAAAANLSVASSVAVGYVTLLSLDEQL
HCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVTQQTLTSREDAWRLAKRQFETGYTSRLELMQADSELRSTRAQIPPLQHQIAQQENALS
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEE
VLLGDNPGAVKRGEFAQLTPLRLPSQLPSTLLNRRPDIAQAERQLVAADATLASSQAQLL
EEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHC
PSINLTATGSLQDRTLPDLLDNPLRLWSLGGSILAPLLNRQALNAQVDVSMAQRNQALYS
CCCCEEECCCCCCCCCHHHHCCCHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YEKTVRSAFKEVNDSLDAISRYGEQLTELQEQETVAQETLRIAQNRYRNGYSSYLDVLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
QRTLFSTQLSVVQVKNNLLLAQIALYRALGGGWSDSSGS
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]