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The map label for this gene is yoaD [H]

Identifier: 238893669

GI number: 238893669

Start: 1531402

End: 1532481

Strand: Direct

Name: yoaD [H]

Synonym: KP1_1587

Alternate gene names: 238893669

Gene position: 1531402-1532481 (Clockwise)

Preceding gene: 238893668

Following gene: 238893675

Centisome position: 29.18

GC content: 64.07

Gene sequence:

>1080_bases
ATGGCAGGCCAGACAGGCCGCCCTTCAACCTCTTTCTCAGGACCTACAACCATGAAAATCGTTTTTACCGCCGAGCATGC
TGGCGACCTTAGCGCCTTCCACCAGCTGGGCGAGCTGCTGGTGGAGGGGTGGGCGCTGGGCAAACCGAAACTCAGCGCCG
GTCAGCTGATTGAGCTGGCCCACGACGCCGAGGTGCTGGTGACCAGCTATGATGAGGTGACGGACGCGGTGATAGCCGCC
TGTCCGCGCCTGCAGGTGATCGCCTGCACCCGCGCGAATCCGGTGAATATTGATACCCAGGCCGCCCAGGCGCGCGGCAT
CCGGGTGCTCTACACCCCGGGGCGCAATGCCGATGCCGCCGCGGAGCTGACGCTTGGCCTGATGCTCAGCCTGGCGCGCC
ATATTCCGCAATCCCATGCGGCGCTGAAGCGGGGGGAATTCACGCAGGCTGACAATGCCGCCGCCGCCACCCAGCAGGGT
CTGCGGCGGGATGTGGTCTGGGACGTGAGCCCGGAAAGCCCCTATGAAGTGTTTAAAGGCAGTGAACTGCGCAATAAAAC
GCTGGGGCTGGTGGGGTATGGCAACATTGGCCGCCGGGTGGCGCGTATCGCCCGGGCGTTCGGCATGGCGGTGTTGGTGG
TGGATCCGTTCGTCGCCGCAGAGGATATCAATGAGCCAGGGCTACAGAAAACGACCCTGGAGGCGCTGTTCCGCGAGGCG
GATATCGTCAGCCTGCACCTGAGCAGCGGGCCGCACAGCGACGGGCTGGTGAATGCCGCGCTGTTAAACAGCATGAAGCC
CGGCGCGCTGCTGATCAATACCTCTCGCGCGGCGGTGGTCGACGAGGCAGCGCTGATTGATGCCCTGCGCCACGGCCCTC
TGGGCGGCGCGGCGCTGGATGTCTATCACCGCGAACCGCTGTGGCGCGATCACCCTTTTGTCATCGAGTTCGATAACGTG
ATTATCACGCCGCATATCGCCGGAGCCACCCGGGAGAGTATCGCCAAACACACGGCGATGATTGCCGCCGATCTTCAGCG
CTATGTCGCCGGGGAGCCGCTGCTGTACCAGTGGCGTTAA

Upstream 100 bases:

>100_bases
CGCTGGCGCAGACCCGCTATCAGCCGCGCCCTCAGGCGCATGCCCGCTACGCGGCGCTGTATCCCGTTTACCGCCTGCTG
CGCGAGCAGATGCTGCCGGT

Downstream 100 bases:

>100_bases
GCCGCTTGCGCCGCCGCTTTCTGCAGCGGCGCGGGTCCATCGTCAGGGGCGATGGGCCGCCATAAAAGCCTGGGTCTGTT
CCCAGTCCACTACCCCGGCA

Product: putative D-3-phosphoglycerate dehydrogenase

Products: 3-phosphohydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MAGQTGRPSTSFSGPTTMKIVFTAEHAGDLSAFHQLGELLVEGWALGKPKLSAGQLIELAHDAEVLVTSYDEVTDAVIAA
CPRLQVIACTRANPVNIDTQAAQARGIRVLYTPGRNADAAAELTLGLMLSLARHIPQSHAALKRGEFTQADNAAAATQQG
LRRDVVWDVSPESPYEVFKGSELRNKTLGLVGYGNIGRRVARIARAFGMAVLVVDPFVAAEDINEPGLQKTTLEALFREA
DIVSLHLSSGPHSDGLVNAALLNSMKPGALLINTSRAAVVDEAALIDALRHGPLGGAALDVYHREPLWRDHPFVIEFDNV
IITPHIAGATRESIAKHTAMIAADLQRYVAGEPLLYQWR

Sequences:

>Translated_359_residues
MAGQTGRPSTSFSGPTTMKIVFTAEHAGDLSAFHQLGELLVEGWALGKPKLSAGQLIELAHDAEVLVTSYDEVTDAVIAA
CPRLQVIACTRANPVNIDTQAAQARGIRVLYTPGRNADAAAELTLGLMLSLARHIPQSHAALKRGEFTQADNAAAATQQG
LRRDVVWDVSPESPYEVFKGSELRNKTLGLVGYGNIGRRVARIARAFGMAVLVVDPFVAAEDINEPGLQKTTLEALFREA
DIVSLHLSSGPHSDGLVNAALLNSMKPGALLINTSRAAVVDEAALIDALRHGPLGGAALDVYHREPLWRDHPFVIEFDNV
IITPHIAGATRESIAKHTAMIAADLQRYVAGEPLLYQWR
>Mature_358_residues
AGQTGRPSTSFSGPTTMKIVFTAEHAGDLSAFHQLGELLVEGWALGKPKLSAGQLIELAHDAEVLVTSYDEVTDAVIAAC
PRLQVIACTRANPVNIDTQAAQARGIRVLYTPGRNADAAAELTLGLMLSLARHIPQSHAALKRGEFTQADNAAAATQQGL
RRDVVWDVSPESPYEVFKGSELRNKTLGLVGYGNIGRRVARIARAFGMAVLVVDPFVAAEDINEPGLQKTTLEALFREAD
IVSLHLSSGPHSDGLVNAALLNSMKPGALLINTSRAAVVDEAALIDALRHGPLGGAALDVYHREPLWRDHPFVIEFDNVI
ITPHIAGATRESIAKHTAMIAADLQRYVAGEPLLYQWR

Specific function: Serine biosynthesis; first step. [C]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=308, Percent_Identity=35.3896103896104, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI61743967, Length=258, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI4557497, Length=258, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=7e-29,
Organism=Homo sapiens, GI145580578, Length=258, Percent_Identity=32.1705426356589, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI4557499, Length=258, Percent_Identity=32.1705426356589, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI145580575, Length=258, Percent_Identity=32.1705426356589, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI6912396, Length=317, Percent_Identity=28.7066246056782, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1789279, Length=310, Percent_Identity=34.1935483870968, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1787645, Length=284, Percent_Identity=31.3380281690141, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87082289, Length=273, Percent_Identity=31.1355311355311, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788660, Length=339, Percent_Identity=28.023598820059, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081824, Length=169, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=299, Percent_Identity=32.7759197324415, Blast_Score=147, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI25147481, Length=261, Percent_Identity=30.2681992337165, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322116, Length=316, Percent_Identity=30.379746835443, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320925, Length=316, Percent_Identity=30.6962025316456, Blast_Score=132, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6324055, Length=301, Percent_Identity=25.9136212624585, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324964, Length=314, Percent_Identity=26.1146496815287, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324980, Length=188, Percent_Identity=28.7234042553192, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921140, Length=270, Percent_Identity=32.5925925925926, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24646446, Length=271, Percent_Identity=32.8413284132841, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24646448, Length=271, Percent_Identity=32.8413284132841, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24646452, Length=271, Percent_Identity=32.8413284132841, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24646450, Length=271, Percent_Identity=32.8413284132841, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI62472511, Length=272, Percent_Identity=32.7205882352941, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI28574286, Length=314, Percent_Identity=27.0700636942675, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24585514, Length=284, Percent_Identity=26.7605633802817, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28574282, Length=284, Percent_Identity=26.7605633802817, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45552429, Length=284, Percent_Identity=26.7605633802817, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI28574284, Length=284, Percent_Identity=26.7605633802817, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI45551003, Length=284, Percent_Identity=26.7605633802817, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28571528, Length=318, Percent_Identity=27.3584905660377, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24585516, Length=277, Percent_Identity=22.7436823104693, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.95

Molecular weight: Translated: 38507; Mature: 38376

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGQTGRPSTSFSGPTTMKIVFTAEHAGDLSAFHQLGELLVEGWALGKPKLSAGQLIELA
CCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEHH
HDAEVLVTSYDEVTDAVIAACPRLQVIACTRANPVNIDTQAAQARGIRVLYTPGRNADAA
CCCEEEEECHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCEEEEECCCCCCCHH
AELTLGLMLSLARHIPQSHAALKRGEFTQADNAAAATQQGLRRDVVWDVSPESPYEVFKG
HHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCHHHHCC
SELRNKTLGLVGYGNIGRRVARIARAFGMAVLVVDPFVAAEDINEPGLQKTTLEALFREA
CCCCCCEEEEEEECHHHHHHHHHHHHHCEEEEEECCCHHHHCCCCCCCHHHHHHHHHHCC
DIVSLHLSSGPHSDGLVNAALLNSMKPGALLINTSRAAVVDEAALIDALRHGPLGGAALD
CEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEE
VYHREPLWRDHPFVIEFDNVIITPHIAGATRESIAKHTAMIAADLQRYVAGEPLLYQWR
EECCCCCCCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
>Mature Secondary Structure 
AGQTGRPSTSFSGPTTMKIVFTAEHAGDLSAFHQLGELLVEGWALGKPKLSAGQLIELA
CCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHEEHH
HDAEVLVTSYDEVTDAVIAACPRLQVIACTRANPVNIDTQAAQARGIRVLYTPGRNADAA
CCCEEEEECHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHCCEEEEECCCCCCCHH
AELTLGLMLSLARHIPQSHAALKRGEFTQADNAAAATQQGLRRDVVWDVSPESPYEVFKG
HHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCHHHHCC
SELRNKTLGLVGYGNIGRRVARIARAFGMAVLVVDPFVAAEDINEPGLQKTTLEALFREA
CCCCCCEEEEEEECHHHHHHHHHHHHHCEEEEEECCCHHHHCCCCCCCHHHHHHHHHHCC
DIVSLHLSSGPHSDGLVNAALLNSMKPGALLINTSRAAVVDEAALIDALRHGPLGGAALD
CEEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEE
VYHREPLWRDHPFVIEFDNVIITPHIAGATRESIAKHTAMIAADLQRYVAGEPLLYQWR
EECCCCCCCCCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-phosphoglycerate; NAD+

Specific reaction: 3-phosphoglycerate + NAD+ = 3-phosphohydroxypyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]