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The map label for this gene is glpK2 [H]

Identifier: 238893628

GI number: 238893628

Start: 1483333

End: 1484946

Strand: Reverse

Name: glpK2 [H]

Synonym: KP1_1542

Alternate gene names: 238893628

Gene position: 1484946-1483333 (Counterclockwise)

Preceding gene: 238893629

Following gene: 238893627

Centisome position: 28.29

GC content: 64.56

Gene sequence:

>1614_bases
ATGTCACAACATGGCCTGCACAGACGCAGGAGACGGTGGGAGATATTCCTCGTCTTCGCGGTTCCAGGACACCGAGTGAC
CGAGGAGAGCACGATGCAGGCGACAGCAAAAGAGTTCATCATTGCCCTGGATGAAGGCACCACCAACGCCAAAGCGGTGG
TGCTGGATAGCCGGGGCAAGGTTATCGTCAAATTCTCGCAGCCGCTGGCGATCCAAACGCCGCGCGACGGCTGGGTGGAG
CAATCGGGCGAAGCGCTGGTTACCGCCTCGCTGGCGGTGATCGCCAGCGCGGTGGCCCACGTTGGCGCGGAAAACGTCGC
CGCGCTGGCCATCAGCAACCAGCGGGAAACCGCCATCGGCTGGTATCGCGACAGCGGCGAACCGATTAACGCGGCGATCA
CCTGGCAATGCACCCGCAGCGCCGCGTTCTGCGATACGCTGCGTCACGATCGTCAGGAACAGCGTATTAAACAGGCCACC
GGCCTGCCGATCGCCCCGCTATTCTCGGCCTCCAAAATGCGCTGGCTGCTGGATGCGACTGCGGATGGCCGCCTGCGCGC
CGAACGCGGCGAAATTTGCCTCGGCACCATCGACAGCTGGCTGCTGTGGAATCTGACTGCTGGAGAAGCCTTCTGCTGCG
ACTACTCCAATGCCGCCCGCACCCAGCTGCTGAACCTGCACCGCGGCGAGTGGGACGACGAGATGCTGGCGCTGTTCGGC
ATTCCGCGCGCGGCGTTGCCGGACATTAAATCATCGAGCGGCCTCTTCGGCCATACCAAAGGACTGGCCGCCATTCCGGA
CGGTATTCCGATCATGGCGATGATCGGCGACTCCCACGCGGCACTGTTCGGCCACGCGCTCGGCGAGGCAGGCTGCGTCA
AAGCCACCTACGGCACCGGCTCTTCGGTCATGGCCCCGGTCAAATCGGCGCAGTGCGATATCGACGCGCTGGCGACCACT
GTCGCCTGGCACGATGGCGACCAGCTGGTGTGGGGCCTGGAAGGGAATATTCCCCACACCGGCGATGCTGTCGCCTGGAT
GGCGGACAGCACCGGACTGAGCGAACTCTCTGCCGCCGAGCTGGCCCATGAGCTGAATACCCTCCCCGCCTCCGTCGATT
CAACCCTCGGGGTCTACTTCGTGCCGGCGCTGACCGGTCTTGGCGCGCCCTGGTGGGACGACAGCGCCCGCGGCGTGATT
TGCGGCCTGAGTCGCGGGGTGAAACGCGCGCATCTGATCCGTGCGGCGCTGGAGTCCATCACCTATCAGATAGCCGACGT
GGTGGTGGCGATGCGTCAGCATGAGGAGTTCACCCTGACGGCGTTGATGGTGGACGGCGGGCCGACCAACAATGACTGGC
TGATGCAATACCAGGCCGATCTGCTCGGCTGCCCGGTGATGCGCAGCGACGTGCCGGAGCTGTCCGCCATCGGCGCGGCG
TTGCTGGCGCGCAAAGCGCTCCATCCGGGGTCGACCGCCGATCTGCAGGCCTTCTTAACCGAACACAGCACCTTTCAGCC
CGACATGGCCCGCCACCAGCGTTTACAAACGCGCTGGCAGGAGTGGCGCCATGCGGTCGACCGAACATTATGGAAACCGG
ACTCGCCAGCCTGA

Upstream 100 bases:

>100_bases
ATGATTTTTCCGGTAGCCCGTCACCGGGTTTGTGGCTTAAGGTATCAAATAAATAATCACTATCGAATATATATTCAGTG
ATTAAAAAGATGATAACGAA

Downstream 100 bases:

>100_bases
CCGCCAGACTGCGCGACGACACGCATTCACTTATCACAGGAGAAACCCGGCCCATAACGATATCGCTATCCCCGAATACA
TCAGGGGAAATTTCAGACCG

Product: putative carbohydrate kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase 2; Glycerokinase 2; GK 2 [H]

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MSQHGLHRRRRRWEIFLVFAVPGHRVTEESTMQATAKEFIIALDEGTTNAKAVVLDSRGKVIVKFSQPLAIQTPRDGWVE
QSGEALVTASLAVIASAVAHVGAENVAALAISNQRETAIGWYRDSGEPINAAITWQCTRSAAFCDTLRHDRQEQRIKQAT
GLPIAPLFSASKMRWLLDATADGRLRAERGEICLGTIDSWLLWNLTAGEAFCCDYSNAARTQLLNLHRGEWDDEMLALFG
IPRAALPDIKSSSGLFGHTKGLAAIPDGIPIMAMIGDSHAALFGHALGEAGCVKATYGTGSSVMAPVKSAQCDIDALATT
VAWHDGDQLVWGLEGNIPHTGDAVAWMADSTGLSELSAAELAHELNTLPASVDSTLGVYFVPALTGLGAPWWDDSARGVI
CGLSRGVKRAHLIRAALESITYQIADVVVAMRQHEEFTLTALMVDGGPTNNDWLMQYQADLLGCPVMRSDVPELSAIGAA
LLARKALHPGSTADLQAFLTEHSTFQPDMARHQRLQTRWQEWRHAVDRTLWKPDSPA

Sequences:

>Translated_537_residues
MSQHGLHRRRRRWEIFLVFAVPGHRVTEESTMQATAKEFIIALDEGTTNAKAVVLDSRGKVIVKFSQPLAIQTPRDGWVE
QSGEALVTASLAVIASAVAHVGAENVAALAISNQRETAIGWYRDSGEPINAAITWQCTRSAAFCDTLRHDRQEQRIKQAT
GLPIAPLFSASKMRWLLDATADGRLRAERGEICLGTIDSWLLWNLTAGEAFCCDYSNAARTQLLNLHRGEWDDEMLALFG
IPRAALPDIKSSSGLFGHTKGLAAIPDGIPIMAMIGDSHAALFGHALGEAGCVKATYGTGSSVMAPVKSAQCDIDALATT
VAWHDGDQLVWGLEGNIPHTGDAVAWMADSTGLSELSAAELAHELNTLPASVDSTLGVYFVPALTGLGAPWWDDSARGVI
CGLSRGVKRAHLIRAALESITYQIADVVVAMRQHEEFTLTALMVDGGPTNNDWLMQYQADLLGCPVMRSDVPELSAIGAA
LLARKALHPGSTADLQAFLTEHSTFQPDMARHQRLQTRWQEWRHAVDRTLWKPDSPA
>Mature_536_residues
SQHGLHRRRRRWEIFLVFAVPGHRVTEESTMQATAKEFIIALDEGTTNAKAVVLDSRGKVIVKFSQPLAIQTPRDGWVEQ
SGEALVTASLAVIASAVAHVGAENVAALAISNQRETAIGWYRDSGEPINAAITWQCTRSAAFCDTLRHDRQEQRIKQATG
LPIAPLFSASKMRWLLDATADGRLRAERGEICLGTIDSWLLWNLTAGEAFCCDYSNAARTQLLNLHRGEWDDEMLALFGI
PRAALPDIKSSSGLFGHTKGLAAIPDGIPIMAMIGDSHAALFGHALGEAGCVKATYGTGSSVMAPVKSAQCDIDALATTV
AWHDGDQLVWGLEGNIPHTGDAVAWMADSTGLSELSAAELAHELNTLPASVDSTLGVYFVPALTGLGAPWWDDSARGVIC
GLSRGVKRAHLIRAALESITYQIADVVVAMRQHEEFTLTALMVDGGPTNNDWLMQYQADLLGCPVMRSDVPELSAIGAAL
LARKALHPGSTADLQAFLTEHSTFQPDMARHQRLQTRWQEWRHAVDRTLWKPDSPA

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=522, Percent_Identity=34.6743295019157, Blast_Score=299, Evalue=4e-81,
Organism=Homo sapiens, GI4504007, Length=522, Percent_Identity=34.6743295019157, Blast_Score=299, Evalue=5e-81,
Organism=Homo sapiens, GI42794763, Length=526, Percent_Identity=34.2205323193916, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI41393575, Length=511, Percent_Identity=34.0508806262231, Blast_Score=282, Evalue=5e-76,
Organism=Homo sapiens, GI88196792, Length=513, Percent_Identity=27.8752436647173, Blast_Score=206, Evalue=6e-53,
Organism=Escherichia coli, GI1790361, Length=500, Percent_Identity=40.6, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1790005, Length=506, Percent_Identity=25.098814229249, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789168, Length=462, Percent_Identity=24.4588744588745, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1789987, Length=515, Percent_Identity=25.0485436893204, Blast_Score=90, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17535599, Length=508, Percent_Identity=31.8897637795276, Blast_Score=258, Evalue=5e-69,
Organism=Caenorhabditis elegans, GI71986140, Length=531, Percent_Identity=25.2354048964218, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71986147, Length=264, Percent_Identity=30.6818181818182, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321755, Length=498, Percent_Identity=31.1244979919679, Blast_Score=209, Evalue=6e-55,
Organism=Drosophila melanogaster, GI17864214, Length=513, Percent_Identity=35.2826510721248, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24654573, Length=441, Percent_Identity=36.281179138322, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24655555, Length=526, Percent_Identity=33.2699619771863, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24655560, Length=526, Percent_Identity=33.2699619771863, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24655570, Length=526, Percent_Identity=33.2699619771863, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24655574, Length=526, Percent_Identity=33.2699619771863, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24655565, Length=526, Percent_Identity=33.2699619771863, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI19922058, Length=494, Percent_Identity=27.3279352226721, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24652871, Length=492, Percent_Identity=27.4390243902439, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24656367, Length=516, Percent_Identity=28.2945736434109, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21357867, Length=535, Percent_Identity=27.8504672897196, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24656372, Length=352, Percent_Identity=28.9772727272727, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI45552909, Length=252, Percent_Identity=29.7619047619048, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 58220; Mature: 58088

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHGLHRRRRRWEIFLVFAVPGHRVTEESTMQATAKEFIIALDEGTTNAKAVVLDSRGK
CCCCCHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHEEEEEECCCCCCEEEEEECCCC
VIVKFSQPLAIQTPRDGWVEQSGEALVTASLAVIASAVAHVGAENVAALAISNQRETAIG
EEEEECCCEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEE
WYRDSGEPINAAITWQCTRSAAFCDTLRHDRQEQRIKQATGLPIAPLFSASKMRWLLDAT
EECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEECC
ADGRLRAERGEICLGTIDSWLLWNLTAGEAFCCDYSNAARTQLLNLHRGEWDDEMLALFG
CCCCEEECCCCEEEEECCCEEEEEECCCCEEEECCCCHHHHHHHHHHCCCCCHHHHHHHC
IPRAALPDIKSSSGLFGHTKGLAAIPDGIPIMAMIGDSHAALFGHALGEAGCVKATYGTG
CCHHHCCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCHHHHHHHHCCCCCCEEEECCCC
SSVMAPVKSAQCDIDALATTVAWHDGDQLVWGLEGNIPHTGDAVAWMADSTGLSELSAAE
CCCCCCCHHCCCCHHHHHHEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHH
LAHELNTLPASVDSTLGVYFVPALTGLGAPWWDDSARGVICGLSRGVKRAHLIRAALESI
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECHHCHHHHHHHHHHHHHH
TYQIADVVVAMRQHEEFTLTALMVDGGPTNNDWLMQYQADLLGCPVMRSDVPELSAIGAA
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEHHHHCCCCHHCCCCHHHHHHHH
LLARKALHPGSTADLQAFLTEHSTFQPDMARHQRLQTRWQEWRHAVDRTLWKPDSPA
HHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SQHGLHRRRRRWEIFLVFAVPGHRVTEESTMQATAKEFIIALDEGTTNAKAVVLDSRGK
CCCCHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHEEEEEECCCCCCEEEEEECCCC
VIVKFSQPLAIQTPRDGWVEQSGEALVTASLAVIASAVAHVGAENVAALAISNQRETAIG
EEEEECCCEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEE
WYRDSGEPINAAITWQCTRSAAFCDTLRHDRQEQRIKQATGLPIAPLFSASKMRWLLDAT
EECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEECC
ADGRLRAERGEICLGTIDSWLLWNLTAGEAFCCDYSNAARTQLLNLHRGEWDDEMLALFG
CCCCEEECCCCEEEEECCCEEEEEECCCCEEEECCCCHHHHHHHHHHCCCCCHHHHHHHC
IPRAALPDIKSSSGLFGHTKGLAAIPDGIPIMAMIGDSHAALFGHALGEAGCVKATYGTG
CCHHHCCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCHHHHHHHHCCCCCCEEEECCCC
SSVMAPVKSAQCDIDALATTVAWHDGDQLVWGLEGNIPHTGDAVAWMADSTGLSELSAAE
CCCCCCCHHCCCCHHHHHHEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCHHHHHHH
LAHELNTLPASVDSTLGVYFVPALTGLGAPWWDDSARGVICGLSRGVKRAHLIRAALESI
HHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECHHCHHHHHHHHHHHHHH
TYQIADVVVAMRQHEEFTLTALMVDGGPTNNDWLMQYQADLLGCPVMRSDVPELSAIGAA
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEEEHHHHCCCCHHCCCCHHHHHHHH
LLARKALHPGSTADLQAFLTEHSTFQPDMARHQRLQTRWQEWRHAVDRTLWKPDSPA
HHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]