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The map label for this gene is gcvA [H]

Identifier: 238893620

GI number: 238893620

Start: 1475293

End: 1476186

Strand: Reverse

Name: gcvA [H]

Synonym: KP1_1534

Alternate gene names: 238893620

Gene position: 1476186-1475293 (Counterclockwise)

Preceding gene: 238893622

Following gene: 238893618

Centisome position: 28.13

GC content: 66.89

Gene sequence:

>894_bases
ATGGCAGATTTTCCCCCTGTCGCCAGCCTGCGCAGCTTTGAGGCGGTGGCGCGCCTCGGCAGCGTAACGCAGGCCGCCCA
CGAGATGAGCGTGACCCATTCGGCGGTCAGCCAGCACATCAAACAGCTGGAAGCCCTGGTCGGCGTCACGCTCTTTATCC
GCCACGGCCGCGGAGTGCGCATCACGGAAGAAGGACGGCTCTATGCCCTGCAAATCCGCGAGGCGTTACAGCACATCGCC
GACGCCACGCGCATGGTGCAGATCAAACCCCGCACCCTGGAAGTGACCCTGGCGACGCTCCCCTCCTTTGGCTGCCACTG
GCTGCTGCCGCGGCTGGCGCGCTTTCAGGCGCGGCATCCGCAGATCGCGGTGCGCCTGCTGACCAGCCTGGCGGTGGTTA
ACCTGCAACAGGAGGGTATCGATCTGGCCATTCGCATGGGGCAAGGCGATTGGGAGGGAATGGAAAGCCGGCATCTGTTT
GCCGATGAACAGCTGGTGGTCGCCGCCCCCGGCTATCGCGGCGGTAACCTGCCGACCACCCCGCAGGCGATCGCCGCCAG
CGATATTATTTTTTCCATGGAGTCCTGGAACGCCTGGTGCAGCCAGGCGGGGCTGGAAAAGCCGATCGTCCCCTCAGGCC
TGCGCCTGAACGACTCCAATCTGGTGCTGGAGGCGGTCCGCCTGGGGGCCGGCGTGGCGCTGGAGCGGCGCTCCCTGGTG
GCGGGGGCGATAGCCCGCGGCGAGCTGGTGCAGCTCACCGCCGTCACCGTCCCCTACCCCTGGCACTACTGGCTAACCGT
CTCCCCGCAGGCGGAGAACCGGCCGGAAGTCGCCCGGTTCATCGCCTGGCTGGAGGAGGAGATCGCCCTCTGGCGCCAGC
AGATCAGCGCTTAG

Upstream 100 bases:

>100_bases
GGCGGCATTTAGCCCCTTTTTCAGCCTGTTCGCGGCGGCTATAAAACCCGCTAACCCGGTAGTAACGTTAACGTATCGAC
TCACTGACTGAGAGATCACC

Downstream 100 bases:

>100_bases
CCTTCGGCCGACGGCGGCAGGCCCGTCCGGGCGGCGTGCTCCTGGGAGAGACGCCAGAAATCGTGCCCGGCGGGATACTG
ATAGAGGCGCAGGGAAAACT

Product: putative LysR family regulatory protein

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MADFPPVASLRSFEAVARLGSVTQAAHEMSVTHSAVSQHIKQLEALVGVTLFIRHGRGVRITEEGRLYALQIREALQHIA
DATRMVQIKPRTLEVTLATLPSFGCHWLLPRLARFQARHPQIAVRLLTSLAVVNLQQEGIDLAIRMGQGDWEGMESRHLF
ADEQLVVAAPGYRGGNLPTTPQAIAASDIIFSMESWNAWCSQAGLEKPIVPSGLRLNDSNLVLEAVRLGAGVALERRSLV
AGAIARGELVQLTAVTVPYPWHYWLTVSPQAENRPEVARFIAWLEEEIALWRQQISA

Sequences:

>Translated_297_residues
MADFPPVASLRSFEAVARLGSVTQAAHEMSVTHSAVSQHIKQLEALVGVTLFIRHGRGVRITEEGRLYALQIREALQHIA
DATRMVQIKPRTLEVTLATLPSFGCHWLLPRLARFQARHPQIAVRLLTSLAVVNLQQEGIDLAIRMGQGDWEGMESRHLF
ADEQLVVAAPGYRGGNLPTTPQAIAASDIIFSMESWNAWCSQAGLEKPIVPSGLRLNDSNLVLEAVRLGAGVALERRSLV
AGAIARGELVQLTAVTVPYPWHYWLTVSPQAENRPEVARFIAWLEEEIALWRQQISA
>Mature_296_residues
ADFPPVASLRSFEAVARLGSVTQAAHEMSVTHSAVSQHIKQLEALVGVTLFIRHGRGVRITEEGRLYALQIREALQHIAD
ATRMVQIKPRTLEVTLATLPSFGCHWLLPRLARFQARHPQIAVRLLTSLAVVNLQQEGIDLAIRMGQGDWEGMESRHLFA
DEQLVVAAPGYRGGNLPTTPQAIAASDIIFSMESWNAWCSQAGLEKPIVPSGLRLNDSNLVLEAVRLGAGVALERRSLVA
GAIARGELVQLTAVTVPYPWHYWLTVSPQAENRPEVARFIAWLEEEIALWRQQISA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=284, Percent_Identity=35.5633802816901, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=30.7167235494881, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1786448, Length=294, Percent_Identity=30.6122448979592, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1786401, Length=266, Percent_Identity=30.0751879699248, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1789639, Length=248, Percent_Identity=27.0161290322581, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=25.3378378378378, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787128, Length=242, Percent_Identity=30.5785123966942, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=37.0689655172414, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789440, Length=261, Percent_Identity=26.0536398467433, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87081978, Length=261, Percent_Identity=27.9693486590038, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=237, Percent_Identity=23.2067510548523, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32794; Mature: 32663

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADFPPVASLRSFEAVARLGSVTQAAHEMSVTHSAVSQHIKQLEALVGVTLFIRHGRGVR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEE
ITEEGRLYALQIREALQHIADATRMVQIKPRTLEVTLATLPSFGCHWLLPRLARFQARHP
EECCCCEEEHHHHHHHHHHHHHHEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCH
QIAVRLLTSLAVVNLQQEGIDLAIRMGQGDWEGMESRHLFADEQLVVAAPGYRGGNLPTT
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCC
PQAIAASDIIFSMESWNAWCSQAGLEKPIVPSGLRLNDSNLVLEAVRLGAGVALERRSLV
CHHHHHHHHEEEHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCHHHHHHH
AGAIARGELVQLTAVTVPYPWHYWLTVSPQAENRPEVARFIAWLEEEIALWRQQISA
HHHHCCCCEEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADFPPVASLRSFEAVARLGSVTQAAHEMSVTHSAVSQHIKQLEALVGVTLFIRHGRGVR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEE
ITEEGRLYALQIREALQHIADATRMVQIKPRTLEVTLATLPSFGCHWLLPRLARFQARHP
EECCCCEEEHHHHHHHHHHHHHHEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCH
QIAVRLLTSLAVVNLQQEGIDLAIRMGQGDWEGMESRHLFADEQLVVAAPGYRGGNLPTT
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEECCCEEEEECCCCCCCCCCCC
PQAIAASDIIFSMESWNAWCSQAGLEKPIVPSGLRLNDSNLVLEAVRLGAGVALERRSLV
CHHHHHHHHEEEHHHHHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHCCCCCHHHHHHH
AGAIARGELVQLTAVTVPYPWHYWLTVSPQAENRPEVARFIAWLEEEIALWRQQISA
HHHHCCCCEEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]