Click here to switch to the map view.

The map label for this gene is leuO [H]

Identifier: 238893057

GI number: 238893057

Start: 881354

End: 882322

Strand: Direct

Name: leuO [H]

Synonym: KP1_0897

Alternate gene names: 238893057

Gene position: 881354-882322 (Clockwise)

Preceding gene: 238893048

Following gene: 238893058

Centisome position: 16.79

GC content: 51.08

Gene sequence:

>969_bases
GTGACGGCGGAATTAGATATGTTAATTGAAACTCCAGAATATAAATCAGCCCCCGGACAGGAAGTCGTTAAACCTCAACT
ACGGATGGTTGATCTCAATCTGCTTACCGTTTTTGATGCGGTTATGCAAGAGCAGAATATTACTCGTGCCGCCCATACGC
TCGGCATGTCACAACCCGCGGTGAGCAATGCGGTTTCACGACTGAAAGTGATGTTTAATGACGAGCTTTTTGTGCGCTAT
GGCCGCGGTATTCAGCCGACGGCGCGCGCCTATCAGCTGTTTGGCTCGGTACGCCAGGCGCTGCAGCTGGTCCAAAACGA
ATTGCCAGGCTCCGGTTTTGAACCGCTGAGCAGTGAGCGGGTGTTCCATCTGTGCGTCTGCAGCCCGCTGGATAACATCC
TGACGTCGGTTATTTTTAATAAAGTTGCAGAGATAGCACCCAATATTCATTTAGTCTTTAAGTCTGCGCTGAATCAAAAT
ACGGAACATCAGCTACGTTATCAGGAAACAGAATTCGTAATCGGATATGAAGAGTTCCGTCGCCCTGAGTTTGCCTGCGT
TCCGTTATTTAAAGACGAAATGGTCCTGGTCGCCAGTCGCAACCATCCGCGTATTTCCGGGCCGATGATGGAGGCGGATA
TTTATCGCGAGGAGCATGCCGCCGTTGCTCTTGACCGGTATGCCTCATTTAGTTTGCCGTGGTATGACACGGCCGATAAA
CAAGCGCGTATCGCCTATCAGGGTAATGCGATGGTCAGCGTATTAAACGTAGTATCGCAAACCCAGATGGTGGCCATTGC
CCCGCGCTGGCTGGCAGATGAATTTGCTGACAAGCTTGCCCTGCAAATATTACCTTTGCCGTTGAAGGTCAACAGTCGGA
CCTGCTATCTCTCCTGGCACGAAGCCGCTGGTCGTGATAAAGGCCACCAGTGGATGGAAGAGCTGCTGGTGGACATTTGC
AAGCGCTGA

Upstream 100 bases:

>100_bases
TATATTCCTAAAAATTTCTTTTGCTATTTTGTAAATGTCATTTGCATGGTAAATGATATTTTCGGCGCTAATTATAGCGG
TTTAGTGAAGGGAATTCATC

Downstream 100 bases:

>100_bases
CCCGACGACGATAGATAGCAAAGGAGCTTCGGCTCCTTTTTTTATATGACATAAATCAAATAAAGCAGAATTTTATTCTG
TTTACCTTGCGCGAGGAGTT

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTAELDMLIETPEYKSAPGQEVVKPQLRMVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVSRLKVMFNDELFVRY
GRGIQPTARAYQLFGSVRQALQLVQNELPGSGFEPLSSERVFHLCVCSPLDNILTSVIFNKVAEIAPNIHLVFKSALNQN
TEHQLRYQETEFVIGYEEFRRPEFACVPLFKDEMVLVASRNHPRISGPMMEADIYREEHAAVALDRYASFSLPWYDTADK
QARIAYQGNAMVSVLNVVSQTQMVAIAPRWLADEFADKLALQILPLPLKVNSRTCYLSWHEAAGRDKGHQWMEELLVDIC
KR

Sequences:

>Translated_322_residues
MTAELDMLIETPEYKSAPGQEVVKPQLRMVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVSRLKVMFNDELFVRY
GRGIQPTARAYQLFGSVRQALQLVQNELPGSGFEPLSSERVFHLCVCSPLDNILTSVIFNKVAEIAPNIHLVFKSALNQN
TEHQLRYQETEFVIGYEEFRRPEFACVPLFKDEMVLVASRNHPRISGPMMEADIYREEHAAVALDRYASFSLPWYDTADK
QARIAYQGNAMVSVLNVVSQTQMVAIAPRWLADEFADKLALQILPLPLKVNSRTCYLSWHEAAGRDKGHQWMEELLVDIC
KR
>Mature_321_residues
TAELDMLIETPEYKSAPGQEVVKPQLRMVDLNLLTVFDAVMQEQNITRAAHTLGMSQPAVSNAVSRLKVMFNDELFVRYG
RGIQPTARAYQLFGSVRQALQLVQNELPGSGFEPLSSERVFHLCVCSPLDNILTSVIFNKVAEIAPNIHLVFKSALNQNT
EHQLRYQETEFVIGYEEFRRPEFACVPLFKDEMVLVASRNHPRISGPMMEADIYREEHAAVALDRYASFSLPWYDTADKQ
ARIAYQGNAMVSVLNVVSQTQMVAIAPRWLADEFADKLALQILPLPLKVNSRTCYLSWHEAAGRDKGHQWMEELLVDICK
R

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=302, Percent_Identity=83.7748344370861, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1786820, Length=282, Percent_Identity=24.822695035461, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081766, Length=297, Percent_Identity=26.9360269360269, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36532; Mature: 36401

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAELDMLIETPEYKSAPGQEVVKPQLRMVDLNLLTVFDAVMQEQNITRAAHTLGMSQPA
CCCCEEEEEECCCCCCCCCHHHHCCCHHEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCH
VSNAVSRLKVMFNDELFVRYGRGIQPTARAYQLFGSVRQALQLVQNELPGSGFEPLSSER
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VFHLCVCSPLDNILTSVIFNKVAEIAPNIHLVFKSALNQNTEHQLRYQETEFVIGYEEFR
EEEEEECCCHHHHHHHHHHHHHHHHCCCHHEEEHHHHCCCCHHHHEEECCEEEEEHHHHC
RPEFACVPLFKDEMVLVASRNHPRISGPMMEADIYREEHAAVALDRYASFSLPWYDTADK
CCCEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
QARIAYQGNAMVSVLNVVSQTQMVAIAPRWLADEFADKLALQILPLPLKVNSRTCYLSWH
CEEEEECCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHEECCCEEECCEEEEEEEH
EAAGRDKGHQWMEELLVDICKR
HHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAELDMLIETPEYKSAPGQEVVKPQLRMVDLNLLTVFDAVMQEQNITRAAHTLGMSQPA
CCCEEEEEECCCCCCCCCHHHHCCCHHEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCH
VSNAVSRLKVMFNDELFVRYGRGIQPTARAYQLFGSVRQALQLVQNELPGSGFEPLSSER
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
VFHLCVCSPLDNILTSVIFNKVAEIAPNIHLVFKSALNQNTEHQLRYQETEFVIGYEEFR
EEEEEECCCHHHHHHHHHHHHHHHHCCCHHEEEHHHHCCCCHHHHEEECCEEEEEHHHHC
RPEFACVPLFKDEMVLVASRNHPRISGPMMEADIYREEHAAVALDRYASFSLPWYDTADK
CCCEEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
QARIAYQGNAMVSVLNVVSQTQMVAIAPRWLADEFADKLALQILPLPLKVNSRTCYLSWH
CEEEEECCCHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHEECCCEEECCEEEEEEEH
EAAGRDKGHQWMEELLVDICKR
HHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3519576 [H]