Click here to switch to the map view.

The map label for this gene is yafC [C]

Identifier: 238028800

GI number: 238028800

Start: 3715265

End: 3716233

Strand: Reverse

Name: yafC [C]

Synonym: bglu_1g32640

Alternate gene names: 238028800

Gene position: 3716233-3715265 (Counterclockwise)

Preceding gene: 238028801

Following gene: 238028798

Centisome position: 95.13

GC content: 69.35

Gene sequence:

>969_bases
ATGGATACCCTCGTCAGCATGAATGTATTTCGCTATGTCGTTGAAGTCGGCAGCTTCGTCGGCGCAGCGGAACGCATGCA
GATGTCGGCGGCGATGGCGAGCAAGCACGTGATGCATCTCGAGCAGCAGCTCGGCGCGCGGCTGCTGCATCGGACCACAC
GCCGCGTCGCACCGACCGAGGCGGGGCGCGAATACTACGAGCGGCTCGTGCAGGCATTGACCGAACTCGACGAGGCGGGC
CAGGCGGTGGGCGCGGCCAGCATCGTGCCGCAGGGCCGCCTGCGCGTGACCTCGCTGTCCGCGTTCGGGCTGCGCCACGT
GATGAGCGCCGTGACCGACTACGCGGCGCGCTATGCCGACGTGACCGTCGAGATCACGCTGTCCGATCGCGTGGTGGAGC
TGATCGACGAAGGCTACGACGTGGCCGTGCGCGCCGCGCCGTTCGGGCTGAAATCGTCGTCGCTGGTGGCGCGGCAGATC
GCGACCGCGCACATCCTGCTGGTCGCCTCGCCGGCCTACCTCGAGGAACACGGCATGCCCGCCGCGCTGGCCGATCTCGA
GCGGCACAATTACCTGCGCCGCGACACCGGGCCGAGCTCGATCGATTCGGCTGCGTTCGAATCGGCCGCGGCCTCGCGCG
TGACGCTGTCGGGCAACCTGATCGTCAACCACCTCGAGGCGCTGCGCCTGGCGGTGCTCAACGGCAGCGGCATCGCGATG
CTCGGCACCGAAGTGGTGGGCGACGACATCGAGGCCGGCCGTCTGGTGCCGCTGCTGCTCGAGGCGATGCCGCCGCGCGA
GCTGCCGATCTACGCGGTCTACGCGAGCCGCCGGCACGTGTCCGCGAAGGTGCGCTCGTTCGTCGATTTCCTGGCCGACC
GCTTCTCCGGCCAATCGCTGTGCCCGTCGATCGACGAGTATCTGAGGGAGGCCGCCGCGCCGAAGGCGAGGCGGGCCGTC
GCGCGCTGA

Upstream 100 bases:

>100_bases
CGCGTCCATAAAACGACGTTTTGCGTTTGTTGACGTTTTCGCGAGCCAGCCTTTGTTACAGTCTTTTCAATGTGCAATTT
CTGGAACGCGAGCGTTTTTG

Downstream 100 bases:

>100_bases
GCGCGCCGGCGCCCGCGCCCGGCGCGGCCGCCGTGCCCGGCGCGCGGCCGGGCCGGCGCCGCATTCAGGCAAGCCGCGTT
CGGGCAAGCCGCGGCGTCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTEAGREYYERLVQALTELDEAG
QAVGAASIVPQGRLRVTSLSAFGLRHVMSAVTDYAARYADVTVEITLSDRVVELIDEGYDVAVRAAPFGLKSSSLVARQI
ATAHILLVASPAYLEEHGMPAALADLERHNYLRRDTGPSSIDSAAFESAAASRVTLSGNLIVNHLEALRLAVLNGSGIAM
LGTEVVGDDIEAGRLVPLLLEAMPPRELPIYAVYASRRHVSAKVRSFVDFLADRFSGQSLCPSIDEYLREAAAPKARRAV
AR

Sequences:

>Translated_322_residues
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTEAGREYYERLVQALTELDEAG
QAVGAASIVPQGRLRVTSLSAFGLRHVMSAVTDYAARYADVTVEITLSDRVVELIDEGYDVAVRAAPFGLKSSSLVARQI
ATAHILLVASPAYLEEHGMPAALADLERHNYLRRDTGPSSIDSAAFESAAASRVTLSGNLIVNHLEALRLAVLNGSGIAM
LGTEVVGDDIEAGRLVPLLLEAMPPRELPIYAVYASRRHVSAKVRSFVDFLADRFSGQSLCPSIDEYLREAAAPKARRAV
AR
>Mature_322_residues
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTEAGREYYERLVQALTELDEAG
QAVGAASIVPQGRLRVTSLSAFGLRHVMSAVTDYAARYADVTVEITLSDRVVELIDEGYDVAVRAAPFGLKSSSLVARQI
ATAHILLVASPAYLEEHGMPAALADLERHNYLRRDTGPSSIDSAAFESAAASRVTLSGNLIVNHLEALRLAVLNGSGIAM
LGTEVVGDDIEAGRLVPLLLEAMPPRELPIYAVYASRRHVSAKVRSFVDFLADRFSGQSLCPSIDEYLREAAAPKARRAV
AR

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=34.0350877192982, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI87081978, Length=307, Percent_Identity=34.5276872964169, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=30.976430976431, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI145693193, Length=298, Percent_Identity=29.5302013422819, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=29.3103448275862, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789440, Length=289, Percent_Identity=29.0657439446367, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789173, Length=260, Percent_Identity=26.5384615384615, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788481, Length=299, Percent_Identity=23.4113712374582, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788706, Length=260, Percent_Identity=25.3846153846154, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI157672245, Length=262, Percent_Identity=27.4809160305344, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34901; Mature: 34901

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGREYYERLVQALTELDEAGQAVGAASIVPQGRLRVTSLSAFGLRHVMSAVTDYAARYAD
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
VTVEITLSDRVVELIDEGYDVAVRAAPFGLKSSSLVARQIATAHILLVASPAYLEEHGMP
EEEEEEEHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHEEEEECCHHHHHCCCC
AALADLERHNYLRRDTGPSSIDSAAFESAAASRVTLSGNLIVNHLEALRLAVLNGSGIAM
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHCCCCCEEE
LGTEVVGDDIEAGRLVPLLLEAMPPRELPIYAVYASRRHVSAKVRSFVDFLADRFSGQSL
ECHHHHCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
CPSIDEYLREAAAPKARRAVAR
CHHHHHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGREYYERLVQALTELDEAGQAVGAASIVPQGRLRVTSLSAFGLRHVMSAVTDYAARYAD
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
VTVEITLSDRVVELIDEGYDVAVRAAPFGLKSSSLVARQIATAHILLVASPAYLEEHGMP
EEEEEEEHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHEEEEECCHHHHHCCCC
AALADLERHNYLRRDTGPSSIDSAAFESAAASRVTLSGNLIVNHLEALRLAVLNGSGIAM
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHCCCCCEEE
LGTEVVGDDIEAGRLVPLLLEAMPPRELPIYAVYASRRHVSAKVRSFVDFLADRFSGQSL
ECHHHHCCCCCCCCHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
CPSIDEYLREAAAPKARRAVAR
CHHHHHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]