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The map label for this gene is gcvA [H]

Identifier: 238028742

GI number: 238028742

Start: 3641909

End: 3642910

Strand: Direct

Name: gcvA [H]

Synonym: bglu_1g32060

Alternate gene names: 238028742

Gene position: 3641909-3642910 (Clockwise)

Preceding gene: 238028735

Following gene: 238028753

Centisome position: 93.23

GC content: 73.65

Gene sequence:

>1002_bases
GTGGACGCTCTGAAACTTCCCCCGCTGAACGCGCTGCGCGCGTTCGAGGCCGCGGCCCGCCACGAGAGCTTCTCGCGCGC
GGCCGACGAGCTGTTCGTCACGCACGGCGCGGTCAGCCACCAGGTCCGCGCGCTCGAGGAGGAGCTCGGCGTGCCGCTCT
TCACGCGCAACGGCCGGCGGCTCGCGCTCACCGAGGCCGGCGAGCGCTACGCGCGGCAGGTGCGCGCCGCGCTGACGATG
CTGATGGACGCGACGCGCGAGGTCTGCGCGCCGGACCGGCCGAACCGGCTGATCGTGTCGTCGCTGCAGTCGTTCGCCGC
CCGCTGGATCACGCCGCGGATCGGCTCGTTCATCGACCGGCACCCCGCCATCGACCTCGAACTGAAGTCCACCGATTCGC
TGACCGATTTCTCGCGCGACGACGTCGACGTGGCGATCCGCTTCGGCCGCGGCCACTATCCGGGGCTGTACGTGGAAAAG
CTGTTCGACGAGGCATTTCTGCCGGCCTGCGCGCCGACGCTGAACGGCGGCGTGCTGCCGCGGGAGCCGGCCGACCTGGC
GCGCTACCCGCTGCTGCGCTCCGACGACGAGCTCTGGCGCCCGTGGTTCGACGCGGCCGGCCTCGCCACGCTGACCGAGC
CCAAGCGCGGGCTGCTGTTCCAGGATTCGTCGCAGCTGGTGCAGGCCGCGATCGACGGCCAGGGCATCGCGCTGGTGCGC
CGCTCGCTGGCCGCGGCCGAACTCGAGGCGGGCCGCATCGTGCGTCTGTTCGACATCCTCGGGCCGAGCCCGTGGCACTG
CTATTTCGTCTGTCCGCACTCGCGGATCCAGCTGCCGCGCGTGCAGGCGTTCCGCAGCTGGCTGCTGGAGGAGCTGGCGC
GCTTCGAGCAGAGCTGGGCGCGCGTGATGGCGCGGCCGGCGGCCAGCGCGGCGGCCGCGCGCACGGCGGCGGGCGCAGCC
GGCACGCCCGGCGCAATCGGGCCGGCCGGCGAGGCGGCCTGA

Upstream 100 bases:

>100_bases
ATGCGCTCCGGGAACGGCGGTGGAATGACTACAATCGTAGACACATCAACGGCTTGCGCAAAACGAGACGTTTTCACGTT
CTTGTGAGAAAAATTAGCAA

Downstream 100 bases:

>100_bases
GCCGGGCGCGAGCGCCGGCAGCAAGGCAGCGGCGCGGGCAAGGCTGCCCGGCAAGGGCGCAAGCAAGACGCGGCGGCGCG
CGGGCGGCGCCCGCCGGCGC

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MDALKLPPLNALRAFEAAARHESFSRAADELFVTHGAVSHQVRALEEELGVPLFTRNGRRLALTEAGERYARQVRAALTM
LMDATREVCAPDRPNRLIVSSLQSFAARWITPRIGSFIDRHPAIDLELKSTDSLTDFSRDDVDVAIRFGRGHYPGLYVEK
LFDEAFLPACAPTLNGGVLPREPADLARYPLLRSDDELWRPWFDAAGLATLTEPKRGLLFQDSSQLVQAAIDGQGIALVR
RSLAAAELEAGRIVRLFDILGPSPWHCYFVCPHSRIQLPRVQAFRSWLLEELARFEQSWARVMARPAASAAAARTAAGAA
GTPGAIGPAGEAA

Sequences:

>Translated_333_residues
MDALKLPPLNALRAFEAAARHESFSRAADELFVTHGAVSHQVRALEEELGVPLFTRNGRRLALTEAGERYARQVRAALTM
LMDATREVCAPDRPNRLIVSSLQSFAARWITPRIGSFIDRHPAIDLELKSTDSLTDFSRDDVDVAIRFGRGHYPGLYVEK
LFDEAFLPACAPTLNGGVLPREPADLARYPLLRSDDELWRPWFDAAGLATLTEPKRGLLFQDSSQLVQAAIDGQGIALVR
RSLAAAELEAGRIVRLFDILGPSPWHCYFVCPHSRIQLPRVQAFRSWLLEELARFEQSWARVMARPAASAAAARTAAGAA
GTPGAIGPAGEAA
>Mature_333_residues
MDALKLPPLNALRAFEAAARHESFSRAADELFVTHGAVSHQVRALEEELGVPLFTRNGRRLALTEAGERYARQVRAALTM
LMDATREVCAPDRPNRLIVSSLQSFAARWITPRIGSFIDRHPAIDLELKSTDSLTDFSRDDVDVAIRFGRGHYPGLYVEK
LFDEAFLPACAPTLNGGVLPREPADLARYPLLRSDDELWRPWFDAAGLATLTEPKRGLLFQDSSQLVQAAIDGQGIALVR
RSLAAAELEAGRIVRLFDILGPSPWHCYFVCPHSRIQLPRVQAFRSWLLEELARFEQSWARVMARPAASAAAARTAAGAA
GTPGAIGPAGEAA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=303, Percent_Identity=44.2244224422442, Blast_Score=239, Evalue=2e-64,
Organism=Escherichia coli, GI1786448, Length=301, Percent_Identity=36.2126245847176, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=30.3754266211604, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=28.3783783783784, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786401, Length=299, Percent_Identity=29.4314381270903, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=37.5, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081978, Length=262, Percent_Identity=27.8625954198473, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=25.1748251748252, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789440, Length=254, Percent_Identity=26.3779527559055, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787128, Length=139, Percent_Identity=30.2158273381295, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789639, Length=252, Percent_Identity=25.3968253968254, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788887, Length=165, Percent_Identity=30.9090909090909, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790262, Length=213, Percent_Identity=26.7605633802817, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788748, Length=167, Percent_Identity=28.7425149700599, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI48994962, Length=120, Percent_Identity=31.6666666666667, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36530; Mature: 36530

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALKLPPLNALRAFEAAARHESFSRAADELFVTHGAVSHQVRALEEELGVPLFTRNGRR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCE
LALTEAGERYARQVRAALTMLMDATREVCAPDRPNRLIVSSLQSFAARWITPRIGSFIDR
EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHC
HPAIDLELKSTDSLTDFSRDDVDVAIRFGRGHYPGLYVEKLFDEAFLPACAPTLNGGVLP
CCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
REPADLARYPLLRSDDELWRPWFDAAGLATLTEPKRGLLFQDSSQLVQAAIDGQGIALVR
CCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCHHHHHHHHHCCCHHHHHH
RSLAAAELEAGRIVRLFDILGPSPWHCYFVCPHSRIQLPRVQAFRSWLLEELARFEQSWA
HHHHHHHHCCCCEEHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RVMARPAASAAAARTAAGAAGTPGAIGPAGEAA
HHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDALKLPPLNALRAFEAAARHESFSRAADELFVTHGAVSHQVRALEEELGVPLFTRNGRR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCE
LALTEAGERYARQVRAALTMLMDATREVCAPDRPNRLIVSSLQSFAARWITPRIGSFIDR
EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHC
HPAIDLELKSTDSLTDFSRDDVDVAIRFGRGHYPGLYVEKLFDEAFLPACAPTLNGGVLP
CCCEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
REPADLARYPLLRSDDELWRPWFDAAGLATLTEPKRGLLFQDSSQLVQAAIDGQGIALVR
CCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEECCHHHHHHHHHCCCHHHHHH
RSLAAAELEAGRIVRLFDILGPSPWHCYFVCPHSRIQLPRVQAFRSWLLEELARFEQSWA
HHHHHHHHCCCCEEHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RVMARPAASAAAARTAAGAAGTPGAIGPAGEAA
HHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]