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The map label for this gene is est [H]

Identifier: 238028633

GI number: 238028633

Start: 3521291

End: 3522235

Strand: Reverse

Name: est [H]

Synonym: bglu_1g30970

Alternate gene names: 238028633

Gene position: 3522235-3521291 (Counterclockwise)

Preceding gene: 238028634

Following gene: 238028631

Centisome position: 90.16

GC content: 73.97

Gene sequence:

>945_bases
ATGAGTTGGCAATCCACCTTCGCCTGCTGGCTGTTGCGCCGGCAGATCCGTCCGAAGACGCTCTCGCCCGTGATCGACGT
GACGCAGGCGCGCGTGCTGGCGGCGCGCCGGCGGGTGCTGCCCACGCGCCCGCCCGCCGGCTGGCGGCTGCGCGAGTCCG
GCGGCGCGCGGGCCGACGGCTCGCCGCGGGGCGAATGGCTCGAGCCGGCCGACGGCCGTGCGACGCCGACGCTGCTGTAT
TTCCACGGCGGCGGCTACTATTTCTGCTCGCCGGCCACGCATCGCCCGCTGGTGTTCGCGCTGAGCCGCAAGCTCGGCGC
GCGCTCGTTCTCGGTCGATTACCGGCTCGCGCCCGAGCATCCGTTCCCGGCCGCGCACGACGACGCACTCGCCGCCTATG
CCGCGCTGCTCGAGGCGGGCGTCGAACCGGCCTCGATCCTGATCGGCGGCGACTCGGCGGGCGGCGGCCTCGCGCTCGCG
CTGCTGGTCGCACTGCGTGATCGCGGCGCGCCCCTGCCGGCCGGCGCGCTGCTGTTTTCGCCATGGACGGATCTGGCCGC
GACGGGCGACACGCTGCGCAGCAACGACGGCCTTGATTCGATGTTCTCCGGGGCGGCGCTGGGCCGTGCCGCGCGCCTGT
ATATCGGCGGCGCCAGTCCCACCGATCCCTACCTGTCCCCGCTCTATGCCGATCCGGCCGGGCTGCCGCCGCTGCTGATC
CAGGTCGGCAGCACCGAAGTGCTGCTCGACGATTCGCGCCGCTTCGCCGAGCGCGCACAGGCGGCCGGCGTCGCTACCGA
ACTGCAGATCTGGCCCGGCATGCCGCACGTCTGGCAGATGGCCGTGCCGTTCATGCCCGAGGCGAGCCGCGCGCTCGATC
AGGCCGCCGCGTTCGGACGTCGTCTGGTCGAGCGCGCGGCGCAGCCGGCGCCGGTGGCGGCCTAG

Upstream 100 bases:

>100_bases
GAACCGGCCAACTGAGGCGCCGCGGCCGCCTTCCAGGCCGGGCGGGCGTCTCACGCGTCCGGCACATGAACCATTCGTCC
CGCGCTCCGCAGGAGGATCG

Downstream 100 bases:

>100_bases
ATCGAGAGCGTGCGGTAGGCGGCCTCGACTGCCGCCTGGCCATAGCGGCGTTCGAGCCGCCGGATCGCGAAGTGGGCGTG
CGCGGCCTGCTGGAAGTGGT

Product: Esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSWQSTFACWLLRRQIRPKTLSPVIDVTQARVLAARRRVLPTRPPAGWRLRESGGARADGSPRGEWLEPADGRATPTLLY
FHGGGYYFCSPATHRPLVFALSRKLGARSFSVDYRLAPEHPFPAAHDDALAAYAALLEAGVEPASILIGGDSAGGGLALA
LLVALRDRGAPLPAGALLFSPWTDLAATGDTLRSNDGLDSMFSGAALGRAARLYIGGASPTDPYLSPLYADPAGLPPLLI
QVGSTEVLLDDSRRFAERAQAAGVATELQIWPGMPHVWQMAVPFMPEASRALDQAAAFGRRLVERAAQPAPVAA

Sequences:

>Translated_314_residues
MSWQSTFACWLLRRQIRPKTLSPVIDVTQARVLAARRRVLPTRPPAGWRLRESGGARADGSPRGEWLEPADGRATPTLLY
FHGGGYYFCSPATHRPLVFALSRKLGARSFSVDYRLAPEHPFPAAHDDALAAYAALLEAGVEPASILIGGDSAGGGLALA
LLVALRDRGAPLPAGALLFSPWTDLAATGDTLRSNDGLDSMFSGAALGRAARLYIGGASPTDPYLSPLYADPAGLPPLLI
QVGSTEVLLDDSRRFAERAQAAGVATELQIWPGMPHVWQMAVPFMPEASRALDQAAAFGRRLVERAAQPAPVAA
>Mature_313_residues
SWQSTFACWLLRRQIRPKTLSPVIDVTQARVLAARRRVLPTRPPAGWRLRESGGARADGSPRGEWLEPADGRATPTLLYF
HGGGYYFCSPATHRPLVFALSRKLGARSFSVDYRLAPEHPFPAAHDDALAAYAALLEAGVEPASILIGGDSAGGGLALAL
LVALRDRGAPLPAGALLFSPWTDLAATGDTLRSNDGLDSMFSGAALGRAARLYIGGASPTDPYLSPLYADPAGLPPLLIQ
VGSTEVLLDDSRRFAERAQAAGVATELQIWPGMPHVWQMAVPFMPEASRALDQAAAFGRRLVERAAQPAPVAA

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=153, Percent_Identity=30.718954248366, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI206597554, Length=94, Percent_Identity=45.7446808510638, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1786682, Length=232, Percent_Identity=29.3103448275862, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17567059, Length=259, Percent_Identity=26.6409266409266, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33394; Mature: 33263

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQSTFACWLLRRQIRPKTLSPVIDVTQARVLAARRRVLPTRPPAGWRLRESGGARADG
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCC
SPRGEWLEPADGRATPTLLYFHGGGYYFCSPATHRPLVFALSRKLGARSFSVDYRLAPEH
CCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCHHHHHHHHHCCCEEEEEEEECCCC
PFPAAHDDALAAYAALLEAGVEPASILIGGDSAGGGLALALLVALRDRGAPLPAGALLFS
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEC
PWTDLAATGDTLRSNDGLDSMFSGAALGRAARLYIGGASPTDPYLSPLYADPAGLPPLLI
CCHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
QVGSTEVLLDDSRRFAERAQAAGVATELQIWPGMPHVWQMAVPFMPEASRALDQAAAFGR
EECCEEEEECCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
RLVERAAQPAPVAA
HHHHHCCCCCCCCC
>Mature Secondary Structure 
SWQSTFACWLLRRQIRPKTLSPVIDVTQARVLAARRRVLPTRPPAGWRLRESGGARADG
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCC
SPRGEWLEPADGRATPTLLYFHGGGYYFCSPATHRPLVFALSRKLGARSFSVDYRLAPEH
CCCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCCCHHHHHHHHHCCCEEEEEEEECCCC
PFPAAHDDALAAYAALLEAGVEPASILIGGDSAGGGLALALLVALRDRGAPLPAGALLFS
CCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEC
PWTDLAATGDTLRSNDGLDSMFSGAALGRAARLYIGGASPTDPYLSPLYADPAGLPPLLI
CCHHHHCCCCCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEE
QVGSTEVLLDDSRRFAERAQAAGVATELQIWPGMPHVWQMAVPFMPEASRALDQAAAFGR
EECCEEEEECCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
RLVERAAQPAPVAA
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]