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The map label for this gene is bmr3 [H]

Identifier: 238028631

GI number: 238028631

Start: 3518693

End: 3520285

Strand: Reverse

Name: bmr3 [H]

Synonym: bglu_1g30950

Alternate gene names: 238028631

Gene position: 3520285-3518693 (Counterclockwise)

Preceding gene: 238028633

Following gene: 238028630

Centisome position: 90.11

GC content: 69.68

Gene sequence:

>1593_bases
ATGGTCGTCCATACCGCGGCCCACCATTCGAGTGGGCAAGTCCTGCCGTTTCGGGAATCGCTGCTCGCGATGCTCGGCAT
CTCGTTCGTCACCATGCTGGTGGCCTTCGACCAGACCGTGGTCGGCACCGCGCTGCCGACCATCGTGGCCGAGTTGCACG
GCTTCGATCTCTACGCCTGGGTCGCCACCTCGTACCTGCTGTCGTCGGTGGTCACGGTGCCGATCTTCGGCCGGCTCGGC
GACCATTACGGCCGCAAGCCGTTCGTGATCGTCTCGATCGTGATGTTTACGGCGGCCTCGGTGCTGTGCGGGATGGCCGG
CAGCATGCGCTTTCTGGTGGTTGCGCGCGTGCTGCAGGGCGTCGGCGGCGGGATGCTGGTGGGCACCGCGTTCGCCTGCG
TGCCCGACCTGTTTCCCGAGTCGGTGGTGCGGCTGCGCTGGCAGGTGCTGATCAGCACCGCGTTCGGCATCGCGAACGCG
ATCGGGCCGTCGCTCGGCGGATTCCTGACGCAGTTCTGGGGCTGGCGCTCGGTGTTCTACGTGAACCTGCCGTTCGGGCT
GCTGTCGCTGTACTTCGTGATCCGCTATCTGCCGCATCTGCGCCATGTCCAGCAGGCCGGCAGGATGCGGCTCGACTGGC
CCGGCGCGCTGCTGATCGCGCTCGCGCTCGGCGCGTTCCAGCTGTTCGTCGAATGGCTGCCGCGCGGCTTCCTCGGCTGG
CCGGCCGCACTGTTGGCGGTGGCGGCGTTGGCCTGCCTGCTGCTCTGGCGCTGGGAGCGTCGCATCGAGCACGCGCTGCT
GCCGGCCGACCTGTTCCGCAACCGCTCGCTGGCGATGCTGTTCTGGCTCGCGATCCTGGCCGGCTTCTCGATGTTCTCGC
TGCTGTTTTTCGCGCCGCTGCTGTTCCAGGGCGGCTTCGGGATGTCGCCGAAGGCGGCCGGCGTGGTGATCACGCCGCTG
GTGGTGTTCATCACGATCGGCAGCATCGTGAACGGCCGGATCATCACGCGCATTCCGAGCCCGAACGCGATGCTGCCCGC
CGGCTTCGCGCTGCTCGCGCTGGCCTGCACCGGCATCGCGCTGTCCTCGCGTGTCACGCCGCAGTGGCTGCTGCTCGTGA
TCATGGTGGCGGGCGGCATCGGGCTCGGCTTCGTGCTGCCGAACCTGACCGTGTTCGCGCAGCAGACGGCCGGGCGCGGG
CATCTGGGCATCGCCACCGCGCTGTTGCAATCGCTGCGCATGGTTGGCGGTATGATCGGCACCGCGCTGACCGGCACGCT
CGTCGGCCAGGTCTATGCGGGCGGGGTGCGTGATGCGCTCGGCGCCGAGCATGCGCAGCGCTGGTTCGCGCGGCTGGCCG
ACCCGCAGATCCTGATCGATCGCGCCGCGCAGACGCGCGTCGTCGCCGAGCTGGTGGGGCAGGGCCATCCCGGCGCGCAA
CTGCTCGAGGCCGCGCGCGCGTCGCTGGTCGCGGCGATCCACCTCGGCATCGGGATCGGCGCGGCCGTGGCGGTGCTGGC
GATCTGGCAGAGTTGCCGCGTGCCGCCGATCCGGCTGCGCGGCAAGCTCGAGCCGACCGTCGTCGCCGAGTGA

Upstream 100 bases:

>100_bases
CCCGTGAGATGAGTGAAGCCAGCGCTTCACCAAGCTTGCGGCATTCTGCGCATGCTATCATTTCGAATCGTTGACATGTC
AAACATTGTGCAAGCAAAAG

Downstream 100 bases:

>100_bases
GCGGCGCGGCCGGCGCCCGCGCGCCAAGCGCCGCCCGCGCCGGTCCGCCCGCGAGGCCGGTCCGCGTCATTCACCCGTCA
TCGTTCTGAATCATGGAAGC

Product: EmrB/QacA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MVVHTAAHHSSGQVLPFRESLLAMLGISFVTMLVAFDQTVVGTALPTIVAELHGFDLYAWVATSYLLSSVVTVPIFGRLG
DHYGRKPFVIVSIVMFTAASVLCGMAGSMRFLVVARVLQGVGGGMLVGTAFACVPDLFPESVVRLRWQVLISTAFGIANA
IGPSLGGFLTQFWGWRSVFYVNLPFGLLSLYFVIRYLPHLRHVQQAGRMRLDWPGALLIALALGAFQLFVEWLPRGFLGW
PAALLAVAALACLLLWRWERRIEHALLPADLFRNRSLAMLFWLAILAGFSMFSLLFFAPLLFQGGFGMSPKAAGVVITPL
VVFITIGSIVNGRIITRIPSPNAMLPAGFALLALACTGIALSSRVTPQWLLLVIMVAGGIGLGFVLPNLTVFAQQTAGRG
HLGIATALLQSLRMVGGMIGTALTGTLVGQVYAGGVRDALGAEHAQRWFARLADPQILIDRAAQTRVVAELVGQGHPGAQ
LLEAARASLVAAIHLGIGIGAAVAVLAIWQSCRVPPIRLRGKLEPTVVAE

Sequences:

>Translated_530_residues
MVVHTAAHHSSGQVLPFRESLLAMLGISFVTMLVAFDQTVVGTALPTIVAELHGFDLYAWVATSYLLSSVVTVPIFGRLG
DHYGRKPFVIVSIVMFTAASVLCGMAGSMRFLVVARVLQGVGGGMLVGTAFACVPDLFPESVVRLRWQVLISTAFGIANA
IGPSLGGFLTQFWGWRSVFYVNLPFGLLSLYFVIRYLPHLRHVQQAGRMRLDWPGALLIALALGAFQLFVEWLPRGFLGW
PAALLAVAALACLLLWRWERRIEHALLPADLFRNRSLAMLFWLAILAGFSMFSLLFFAPLLFQGGFGMSPKAAGVVITPL
VVFITIGSIVNGRIITRIPSPNAMLPAGFALLALACTGIALSSRVTPQWLLLVIMVAGGIGLGFVLPNLTVFAQQTAGRG
HLGIATALLQSLRMVGGMIGTALTGTLVGQVYAGGVRDALGAEHAQRWFARLADPQILIDRAAQTRVVAELVGQGHPGAQ
LLEAARASLVAAIHLGIGIGAAVAVLAIWQSCRVPPIRLRGKLEPTVVAE
>Mature_530_residues
MVVHTAAHHSSGQVLPFRESLLAMLGISFVTMLVAFDQTVVGTALPTIVAELHGFDLYAWVATSYLLSSVVTVPIFGRLG
DHYGRKPFVIVSIVMFTAASVLCGMAGSMRFLVVARVLQGVGGGMLVGTAFACVPDLFPESVVRLRWQVLISTAFGIANA
IGPSLGGFLTQFWGWRSVFYVNLPFGLLSLYFVIRYLPHLRHVQQAGRMRLDWPGALLIALALGAFQLFVEWLPRGFLGW
PAALLAVAALACLLLWRWERRIEHALLPADLFRNRSLAMLFWLAILAGFSMFSLLFFAPLLFQGGFGMSPKAAGVVITPL
VVFITIGSIVNGRIITRIPSPNAMLPAGFALLALACTGIALSSRVTPQWLLLVIMVAGGIGLGFVLPNLTVFAQQTAGRG
HLGIATALLQSLRMVGGMIGTALTGTLVGQVYAGGVRDALGAEHAQRWFARLADPQILIDRAAQTRVVAELVGQGHPGAQ
LLEAARASLVAAIHLGIGIGAAVAVLAIWQSCRVPPIRLRGKLEPTVVAE

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=307, Percent_Identity=28.6644951140065, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI87081983, Length=436, Percent_Identity=26.3761467889908, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789042, Length=421, Percent_Identity=23.9904988123515, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788392, Length=321, Percent_Identity=28.0373831775701, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788710, Length=434, Percent_Identity=24.6543778801843, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6319770, Length=431, Percent_Identity=23.4338747099768, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322958, Length=185, Percent_Identity=30.2702702702703, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6323735, Length=313, Percent_Identity=24.2811501597444, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=154, Percent_Identity=32.4675324675325, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=183, Percent_Identity=31.6939890710383, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324954, Length=401, Percent_Identity=22.4438902743142, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56664; Mature: 56664

Theoretical pI: Translated: 11.00; Mature: 11.00

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVHTAAHHSSGQVLPFRESLLAMLGISFVTMLVAFDQTVVGTALPTIVAELHGFDLYAW
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VATSYLLSSVVTVPIFGRLGDHYGRKPFVIVSIVMFTAASVLCGMAGSMRFLVVARVLQG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
VGGGMLVGTAFACVPDLFPESVVRLRWQVLISTAFGIANAIGPSLGGFLTQFWGWRSVFY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEE
VNLPFGLLSLYFVIRYLPHLRHVQQAGRMRLDWPGALLIALALGAFQLFVEWLPRGFLGW
EECCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCH
PAALLAVAALACLLLWRWERRIEHALLPADLFRNRSLAMLFWLAILAGFSMFSLLFFAPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LFQGGFGMSPKAAGVVITPLVVFITIGSIVNGRIITRIPSPNAMLPAGFALLALACTGIA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
LSSRVTPQWLLLVIMVAGGIGLGFVLPNLTVFAQQTAGRGHLGIATALLQSLRMVGGMIG
HCCCCCHHHHHHHHHHHCCCHHHHHHHCHHHEEHHCCCCCCHHHHHHHHHHHHHHHHHHH
TALTGTLVGQVYAGGVRDALGAEHAQRWFARLADPQILIDRAAQTRVVAELVGQGHPGAQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHH
LLEAARASLVAAIHLGIGIGAAVAVLAIWQSCRVPPIRLRGKLEPTVVAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MVVHTAAHHSSGQVLPFRESLLAMLGISFVTMLVAFDQTVVGTALPTIVAELHGFDLYAW
CEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VATSYLLSSVVTVPIFGRLGDHYGRKPFVIVSIVMFTAASVLCGMAGSMRFLVVARVLQG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
VGGGMLVGTAFACVPDLFPESVVRLRWQVLISTAFGIANAIGPSLGGFLTQFWGWRSVFY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEE
VNLPFGLLSLYFVIRYLPHLRHVQQAGRMRLDWPGALLIALALGAFQLFVEWLPRGFLGW
EECCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCH
PAALLAVAALACLLLWRWERRIEHALLPADLFRNRSLAMLFWLAILAGFSMFSLLFFAPL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LFQGGFGMSPKAAGVVITPLVVFITIGSIVNGRIITRIPSPNAMLPAGFALLALACTGIA
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHH
LSSRVTPQWLLLVIMVAGGIGLGFVLPNLTVFAQQTAGRGHLGIATALLQSLRMVGGMIG
HCCCCCHHHHHHHHHHHCCCHHHHHHHCHHHEEHHCCCCCCHHHHHHHHHHHHHHHHHHH
TALTGTLVGQVYAGGVRDALGAEHAQRWFARLADPQILIDRAAQTRVVAELVGQGHPGAQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHH
LLEAARASLVAAIHLGIGIGAAVAVLAIWQSCRVPPIRLRGKLEPTVVAE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]