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The map label for this gene is queF [H]

Identifier: 238028624

GI number: 238028624

Start: 3512024

End: 3512848

Strand: Direct

Name: queF [H]

Synonym: bglu_1g30880

Alternate gene names: 238028624

Gene position: 3512024-3512848 (Clockwise)

Preceding gene: 238028623

Following gene: 238028628

Centisome position: 89.9

GC content: 67.52

Gene sequence:

>825_bases
ATGACTCCCGAACATTCCCCGCTCGGCAAGGCCGCCGTCTACACGACCGACTACGACGCCGCGCTGCTGTTTCCGATCCC
GCGCGCCGCTGCGCGCGAGCAGATCGGCATCGGCGCCACGCTGCCGTTCTTCGGCACCGACATCTGGAACGCCTACGAAC
TGTCGTGGCTCACCCCGCGCGGCAAGCCGCAGATCGCGGTGGCGACCTTCCTGGTGCCGGCCGATTCGCCGAACATCGTC
GAATCGAAGTCGTTCAAGCTCTATCTCGGCTCGTTCGCGCAGACCGCGTTCGATTCGGCCGACGCGGTGCGCGACACGCT
GAAGCGCGACGTGTCGGCCGCCTGCGGCGCGAGCGTGGCAGTGCAGCTGGCCTCGCCGTCCGGGTTCGCGAAGCTGAAGC
TCGAGGAACTCGCCGGCCTGTCGCTCGACCGGCTCGATCTCGACACCGACGTCTATCATCCGGACCCGACGCTGCTGTCG
GCCTCGCACGACGAGTCGCCGGTCGAGGAAACGCTGGTATCGGACCTGCTCAAGTCGAACTGCCCGGTCACGGGCCAGCC
CGACTGGGGCAGCGTGCAGATCCATTACGTCGGCGCGCCGATCGACCACGCGGGGCTGCTGCGCTACCTGATTTCGTTTC
GCAACCACACGGGCTTTCACGAGCAGTGCGTCGAGCGGATCTTCGTCGATGTCCTGCGCGAATGCCGGCCGCTGAAGCTG
GCCGTCTACGCGCGCTACACGCGCCGCGGCGGGCTCGACATCAACCCGTTCCGCACCAACTTCAACCAGCCGCTGCCCGA
CAACGCGCGCACCGCGCGGCAGTAA

Upstream 100 bases:

>100_bases
CGTTCGACCGGTTCCTGGCCGCGCTCGGCTATCCCTATCACGAAGAGACCGCGAACCCGGCCTACCGGCTGTTCCTGGCG
TAATTGCAGAGAGACGCTCG

Downstream 100 bases:

>100_bases
ACGCGGCGGCGGGGCGCGCTCGAAACGACCAACGCGGCAAGCCGGGCGCCGGTGCCCGCCTTGCCGCATTGGTGATCGGC
AAGGGCCCGCCGCGCCGCCT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTPEHSPLGKAAVYTTDYDAALLFPIPRAAAREQIGIGATLPFFGTDIWNAYELSWLTPRGKPQIAVATFLVPADSPNIV
ESKSFKLYLGSFAQTAFDSADAVRDTLKRDVSAACGASVAVQLASPSGFAKLKLEELAGLSLDRLDLDTDVYHPDPTLLS
ASHDESPVEETLVSDLLKSNCPVTGQPDWGSVQIHYVGAPIDHAGLLRYLISFRNHTGFHEQCVERIFVDVLRECRPLKL
AVYARYTRRGGLDINPFRTNFNQPLPDNARTARQ

Sequences:

>Translated_274_residues
MTPEHSPLGKAAVYTTDYDAALLFPIPRAAAREQIGIGATLPFFGTDIWNAYELSWLTPRGKPQIAVATFLVPADSPNIV
ESKSFKLYLGSFAQTAFDSADAVRDTLKRDVSAACGASVAVQLASPSGFAKLKLEELAGLSLDRLDLDTDVYHPDPTLLS
ASHDESPVEETLVSDLLKSNCPVTGQPDWGSVQIHYVGAPIDHAGLLRYLISFRNHTGFHEQCVERIFVDVLRECRPLKL
AVYARYTRRGGLDINPFRTNFNQPLPDNARTARQ
>Mature_273_residues
TPEHSPLGKAAVYTTDYDAALLFPIPRAAAREQIGIGATLPFFGTDIWNAYELSWLTPRGKPQIAVATFLVPADSPNIVE
SKSFKLYLGSFAQTAFDSADAVRDTLKRDVSAACGASVAVQLASPSGFAKLKLEELAGLSLDRLDLDTDVYHPDPTLLSA
SHDESPVEETLVSDLLKSNCPVTGQPDWGSVQIHYVGAPIDHAGLLRYLISFRNHTGFHEQCVERIFVDVLRECRPLKLA
VYARYTRRGGLDINPFRTNFNQPLPDNARTARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=269, Percent_Identity=52.0446096654275, Blast_Score=268, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 30086; Mature: 29955

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEHSPLGKAAVYTTDYDAALLFPIPRAAAREQIGIGATLPFFGTDIWNAYELSWLTPR
CCCCCCCCCCEEEEECCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCC
GKPQIAVATFLVPADSPNIVESKSFKLYLGSFAQTAFDSADAVRDTLKRDVSAACGASVA
CCCCEEEEEEEEECCCCCCCCCCCEEEEECHHHHHHHCHHHHHHHHHHHHHHHHCCCEEE
VQLASPSGFAKLKLEELAGLSLDRLDLDTDVYHPDPTLLSASHDESPVEETLVSDLLKSN
EEEECCCCCEEEEHHHHCCCCCEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCC
CPVTGQPDWGSVQIHYVGAPIDHAGLLRYLISFRNHTGFHEQCVERIFVDVLRECRPLKL
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEE
AVYARYTRRGGLDINPFRTNFNQPLPDNARTARQ
EEEEHHHHCCCCCCCHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPEHSPLGKAAVYTTDYDAALLFPIPRAAAREQIGIGATLPFFGTDIWNAYELSWLTPR
CCCCCCCCCEEEEECCCCCEEEECCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCC
GKPQIAVATFLVPADSPNIVESKSFKLYLGSFAQTAFDSADAVRDTLKRDVSAACGASVA
CCCCEEEEEEEEECCCCCCCCCCCEEEEECHHHHHHHCHHHHHHHHHHHHHHHHCCCEEE
VQLASPSGFAKLKLEELAGLSLDRLDLDTDVYHPDPTLLSASHDESPVEETLVSDLLKSN
EEEECCCCCEEEEHHHHCCCCCEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCC
CPVTGQPDWGSVQIHYVGAPIDHAGLLRYLISFRNHTGFHEQCVERIFVDVLRECRPLKL
CCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEE
AVYARYTRRGGLDINPFRTNFNQPLPDNARTARQ
EEEEHHHHCCCCCCCHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA